##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548193_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556625
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.342846620256008	30.0	25.0	31.0	16.0	33.0
2	27.406172917134516	30.0	25.0	31.0	16.0	33.0
3	27.275512238940042	30.0	25.0	31.0	16.0	34.0
4	31.889933078823265	33.0	30.0	35.0	19.0	37.0
5	33.04730653492028	35.0	32.0	35.0	28.0	37.0
6	31.803190657983382	35.0	32.0	35.0	25.0	37.0
7	33.54566539411633	35.0	33.0	35.0	30.0	37.0
8	32.969557601616884	35.0	33.0	36.0	27.0	37.0
9	35.63171614641814	37.0	34.0	39.0	30.0	39.0
10	35.159306534920276	37.0	34.0	39.0	30.0	39.0
11	35.537609701324946	37.0	35.0	39.0	30.0	39.0
12	35.497067145744445	37.0	34.0	39.0	30.0	39.0
13	35.3318589714799	37.0	34.0	39.0	30.0	39.0
14	35.857884572198515	38.0	34.0	40.0	29.0	41.0
15	36.37750370536717	38.0	35.0	40.0	31.0	41.0
16	36.068085335728725	37.0	34.0	40.0	31.0	41.0
17	35.63255692791377	37.0	34.0	39.0	30.0	40.0
18	35.66383651470918	37.0	34.0	39.0	30.0	40.0
19	35.18710981360881	37.0	34.0	39.0	29.0	40.0
20	35.14862789130923	36.0	34.0	39.0	29.0	40.0
21	35.50177588142825	37.0	34.0	39.0	30.0	40.0
22	36.01709768695262	37.0	34.0	40.0	31.0	40.0
23	36.08819222995733	37.0	34.0	40.0	31.0	41.0
24	36.07149696833595	38.0	35.0	40.0	31.0	41.0
25	35.33737435436784	37.0	34.0	40.0	27.0	41.0
26	35.49047383786212	37.0	34.0	40.0	29.0	41.0
27	35.70406647204132	37.0	34.0	40.0	30.0	41.0
28	35.47950595104424	37.0	34.0	40.0	29.0	41.0
29	35.50373411183472	37.0	34.0	40.0	29.0	41.0
30	34.97195778127105	37.0	34.0	40.0	26.0	41.0
31	35.06657624073658	37.0	34.0	40.0	27.0	41.0
32	34.65893914215136	37.0	34.0	40.0	25.0	41.0
33	34.587178082191784	37.0	34.0	40.0	24.0	41.0
34	34.28788861441725	38.0	34.0	40.0	22.0	41.0
35	34.2232382663373	38.0	34.0	40.0	21.0	41.0
36	33.889325847743095	37.0	33.0	40.0	18.0	41.0
37	34.04908690770267	38.0	34.0	40.0	18.0	41.0
38	33.89925892656636	37.0	33.0	40.0	18.0	41.0
39	33.75206108241635	37.0	33.0	40.0	18.0	41.0
40	33.58941118347182	37.0	33.0	40.0	17.0	41.0
41	33.09507118796317	37.0	32.0	40.0	15.0	41.0
42	33.34974354367842	37.0	33.0	40.0	15.0	41.0
43	33.30901414776555	37.0	33.0	40.0	15.0	41.0
44	33.405623175387376	37.0	33.0	40.0	15.0	41.0
45	33.52082281607905	37.0	33.0	40.0	17.0	41.0
46	33.25443161913317	37.0	33.0	40.0	15.0	41.0
47	33.09100381765102	36.0	32.0	40.0	15.0	40.0
48	33.11934426229508	37.0	32.0	40.0	15.0	40.0
49	33.21880440152706	37.0	33.0	40.0	15.0	40.0
50	33.03021064450932	36.0	32.0	40.0	14.0	40.0
51	30.82026678643611	34.0	28.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	18.0
11	13.0
12	18.0
13	29.0
14	38.0
15	67.0
16	154.0
17	429.0
18	977.0
19	2010.0
20	3647.0
21	5582.0
22	6924.0
23	7613.0
24	8175.0
25	9678.0
26	10962.0
27	11163.0
28	10984.0
29	12038.0
30	15107.0
31	21412.0
32	30099.0
33	44177.0
34	57292.0
35	61070.0
36	81227.0
37	90624.0
38	56700.0
39	8391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.042802604985404	22.997529755221198	23.415764653042892	14.543902986750506
2	28.936357511789808	36.010599595778125	22.235796092521895	12.817246799910173
3	22.557197395014597	35.57619582304065	29.045587244554234	12.821019537390525
4	20.667594879856278	24.909050078598696	40.02335504154503	14.399999999999999
5	27.271861666292384	27.04549741747137	28.92773411183472	16.754906804401525
6	17.371300247024475	40.904738378621154	29.136492252414104	12.587469121940265
7	63.799505951044246	7.72512912643162	23.98526835841006	4.49009656411408
8	58.57749831574219	13.850078598697507	16.807725129126432	10.764697956433865
9	54.0879407141253	8.54201661801033	19.86867280485066	17.5013698630137
10	38.022546597799234	22.35724230855603	23.42995733213564	16.190253761509098
11	30.14686728048507	23.149337525263867	28.623220300920728	18.08057489333034
12	23.805254884347633	17.996137435436786	35.16586570851112	23.032741971704468
13	21.333213563889515	28.230136986301368	37.982304064675496	12.454345385133617
14	14.340175162811589	37.66701100381765	29.125713002470242	18.867100830900515
15	8.927195149337527	23.99407141253088	49.62982259151134	17.448910846620254
16	9.011991915562541	26.564563215809567	37.39914664271278	27.02429822591511
17	9.959667639793397	27.28210195373905	32.589086009431846	30.16914439703571
18	14.130159443072085	24.997260273972604	35.294677745340216	25.577902537615095
19	15.674825960026947	29.512867729620478	31.481877386031886	23.33042892432068
20	19.30437907028969	27.220300920727595	34.38832247922748	19.08699752975522
21	14.921356388951267	34.04105097686953	33.0222322030092	18.015360431169995
22	13.9695486189086	25.044688973725577	32.29894453177633	28.686817875589487
23	13.815225690545699	33.20925218953514	30.298675050527734	22.67684706939142
24	17.385313271951492	25.705097686952616	31.16281158769369	25.746777453402203
25	12.725802829553109	34.107702672355714	28.149112957556703	25.017381540534473
26	12.597529755221199	30.55989220750056	31.401751628115875	25.440826409162366
27	18.35526611273299	31.746148663822144	26.48533572872221	23.41324949472266
28	11.06615764653043	29.405614192679092	37.769413878284304	21.758814282506176
29	13.848461711205928	27.81441724679991	32.10940938693016	26.227711655064002
30	16.293734560970133	28.460992589265665	32.3469121940265	22.898360655737704
31	20.219896698854704	26.742600494048958	28.76299124185942	24.27451156523692
32	19.436424882101953	30.54659779923647	29.3824388052998	20.63453851336178
33	19.532719514933753	23.7366269930384	28.604356613518977	28.12629687850887
34	22.067100830900515	24.541298001347407	30.959443072086234	22.432158095665844
35	18.089737255782616	22.066741522569057	27.59883224792275	32.24468897372558
36	23.98347181675275	23.636559622726253	31.263956883000226	21.11601167752077
37	21.130743319110714	20.0609027621828	34.7294857399506	24.078868178755894
38	20.12971030765776	21.195598472939594	33.043611048731194	25.631080170671456
39	28.272176061082416	18.970222322030093	33.221827981136315	19.53577363575118
40	24.337390523242757	18.316640467100832	34.11704468897373	23.228924320682687
41	25.818100157197392	20.207680215585	25.723781720188637	28.250437907028967
42	25.653177633056366	19.125443521221648	29.84109589041096	25.380282955311028
43	27.674107343364025	18.373231529306086	28.980732090725354	24.971929036604536
44	21.36591062205255	20.70154951717943	31.075858971479903	26.85668088928812
45	19.453492027846394	18.935728722209745	30.68061980687177	30.930159443072085
46	28.43458342690321	20.373860318886145	29.705636649449808	21.485919604760838
47	18.749966314843924	19.01621378845722	38.20291938019313	24.030900516505728
48	20.541477655513138	19.492836290141476	31.049809117448913	28.915876936896474
49	22.641994161239616	13.990388502133394	37.3447114304963	26.0229059061307
50	21.039119694587917	16.310262744217383	34.833505501908824	27.817112059285876
51	18.01625870199865	16.94048955760162	28.047428699752974	36.995823040646755
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1126.0
1	1049.0
2	972.0
3	3814.0
4	6656.0
5	4877.0
6	3098.0
7	3286.5
8	3475.0
9	3818.0
10	4161.0
11	4374.5
12	4588.0
13	4705.0
14	4822.0
15	4842.5
16	4863.0
17	4923.5
18	4984.0
19	5043.0
20	5102.0
21	4683.0
22	4264.0
23	3986.5
24	3709.0
25	3933.0
26	4464.5
27	4772.0
28	5183.5
29	5595.0
30	5660.0
31	5725.0
32	5809.0
33	5893.0
34	6104.0
35	6315.0
36	6810.0
37	7305.0
38	8439.0
39	9573.0
40	13934.5
41	18296.0
42	23632.0
43	28968.0
44	36722.5
45	44477.0
46	78991.0
47	113505.0
48	97394.5
49	81284.0
50	72144.0
51	63004.0
52	50161.0
53	37318.0
54	30195.0
55	23072.0
56	18767.5
57	14463.0
58	12240.5
59	10018.0
60	8850.5
61	7683.0
62	6286.0
63	4889.0
64	4094.5
65	3300.0
66	2575.5
67	1851.0
68	1508.0
69	1165.0
70	987.0
71	809.0
72	708.0
73	607.0
74	463.0
75	271.5
76	224.0
77	160.5
78	97.0
79	70.5
80	44.0
81	40.5
82	37.0
83	32.5
84	28.0
85	16.5
86	5.0
87	4.0
88	3.0
89	1.5
90	0.0
91	2.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	556625.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.60197839192584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9918463627671	25.100865114734074
2	9.58523745536019	6.249954088022623
3	3.9166352595199516	3.8307017429977304
4	2.059257362684124	2.6854345616656805
5	1.3080139098487171	2.1321920612613154
6	0.8662117314923395	1.694412969176755
7	0.6413877179385382	1.4637355964755194
8	0.42838314771788927	1.1172910500291042
9	0.3751970025995889	1.1008948114320427
>10	3.178975360086721	23.601692929178085
>50	0.5564791509303921	11.896464316009931
>100	0.07735060197932443	4.416203839694897
>500	0.006121270660234308	1.416371020314603
>1k	0.0066777498111647	5.638270599377127
>5k	5.564791509303916E-4	0.9127401822982921
>10k+	0.001669437452791175	6.742775117332206
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	13719	2.4646754996631484	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC	12798	2.299214013024927	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	10649	1.913137210869077	No Hit
GAACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	5031	0.9038401077924995	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3982	0.7153828879407141	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC	3927	0.7055019088255109	No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	3816	0.6855602964293734	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTTTGCGT	3245	0.5829777677969908	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT	2991	0.5373456097013249	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	2795	0.5021333932180553	No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	2508	0.45057264765326743	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTA	2355	0.42308556029642935	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT	1501	0.2696609027621828	No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1470	0.26409162362452276	No Hit
ACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1298	0.2331911071187963	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC	1190	0.2137884572198518	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	964	0.17318661576465305	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGT	935	0.167976644958455	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	784	0.14084886593307883	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATG	707	0.12701549517179428	No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	691	0.12414102852009881	No Hit
ACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	678	0.12180552436559622	No Hit
AGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	654	0.117493824388053	No Hit
AAAACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	645	0.1158769368964743	No Hit
AGCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	615	0.11048731192454524	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	578	0.10384010779249944	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.796541657309679E-4	0.0	0.0	0.5513586346283404	0.0
2	1.796541657309679E-4	0.0	0.0	2.3527509544127554	0.0
3	1.796541657309679E-4	0.0	0.0	3.2201212665618684	0.0
4	1.796541657309679E-4	0.0	0.0	5.581675275095441	0.0
5	1.796541657309679E-4	0.0	0.0	10.973096788681788	0.0
6	1.796541657309679E-4	0.0	0.0	13.344531776330564	0.0
7	1.796541657309679E-4	0.0	0.0	15.3316865034808	0.0
8	1.796541657309679E-4	0.0	0.0	19.25766898719964	0.0
9	1.796541657309679E-4	0.0	0.0	20.390388502133394	0.0
10	1.796541657309679E-4	0.0	0.0	24.808084437457893	0.0
11	1.796541657309679E-4	0.0	0.0	28.141387828430272	0.0
12	1.796541657309679E-4	0.0	0.0	33.75288569503705	0.0
13	1.796541657309679E-4	0.0	0.0	35.15239164608129	0.0
14	1.796541657309679E-4	0.0	0.0	35.61751628115877	0.0
15	1.796541657309679E-4	0.0	0.0	36.67567931731417	0.0
16	1.796541657309679E-4	0.0	0.0	38.05290815180777	0.0
17	1.796541657309679E-4	0.0	0.0	39.29216258701999	0.0
18	0.0023355041545025826	0.0	0.0	41.575207725129125	0.0
19	0.0023355041545025826	0.0	0.0	43.15490680440153	0.0
20	0.0023355041545025826	0.0	0.0	44.24343139456546	0.0
21	0.0023355041545025826	0.0	0.0	44.97677969907927	0.0
22	0.0023355041545025826	0.0	0.0	45.63790702896923	0.0
23	0.0023355041545025826	0.0	0.0	46.246934650797215	0.0
24	0.0023355041545025826	0.0	0.0	46.6755894902313	0.0
25	0.0023355041545025826	0.0	0.0	47.01585448012576	0.0
26	0.0023355041545025826	0.0	0.0	47.38396586570851	0.0
27	0.0025151583202335506	0.0	0.0	47.749921401302494	0.0
28	0.0025151583202335506	0.0	0.0	48.03629014147766	0.0
29	0.0025151583202335506	0.0	0.0	48.35805075230182	0.0
30	0.0025151583202335506	0.0	0.0	48.71825735459241	0.0
31	0.0025151583202335506	0.0	0.0	49.05852234448686	0.0
32	0.0025151583202335506	0.0	0.0	49.332135638895124	0.0
33	0.0025151583202335506	0.0	0.0	49.61958230406468	0.0
34	0.0025151583202335506	0.0	0.0	49.915113406692114	0.0
35	0.0025151583202335506	0.0	0.0	50.21926790927465	0.0
36	0.0025151583202335506	0.0	0.0	50.49431843700876	0.0
37	0.0025151583202335506	0.0	0.0	50.77601616887492	0.0
38	0.002694812485964518	0.0	0.0	51.027711655064	0.0
39	0.002694812485964518	0.0	0.0	51.30761284527285	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAT	20	7.030146E-4	45.000004	21
TCGCCAA	20	7.030146E-4	45.000004	40
CAGGTCT	40	6.8048394E-9	45.000004	5
ACTATTA	30	2.163477E-6	45.000004	17
TCGTTTG	20	7.030146E-4	45.000004	12
CGAACTC	20	7.030146E-4	45.000004	13
GTACAAT	20	7.030146E-4	45.000004	24
CATGCTT	20	7.030146E-4	45.000004	10
CGACGGT	20	7.030146E-4	45.000004	28
TGGACAG	20	7.030146E-4	45.000004	1
ACCGCAA	20	7.030146E-4	45.000004	29
CGCATTG	20	7.030146E-4	45.000004	28
CGTTGGT	20	7.030146E-4	45.000004	14
CGACATG	20	7.030146E-4	45.000004	18
CACGACG	20	7.030146E-4	45.000004	26
TAACGAT	20	7.030146E-4	45.000004	13
GCGTTGC	20	7.030146E-4	45.000004	20
GCCGATT	20	7.030146E-4	45.000004	9
ACGATAT	20	7.030146E-4	45.000004	15
AAGCCGT	20	7.030146E-4	45.000004	12
>>END_MODULE