##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548192_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	877689
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.654766095963378	28.0	25.0	31.0	16.0	33.0
2	27.514692561943924	30.0	25.0	31.0	16.0	33.0
3	27.44236056279616	30.0	25.0	31.0	16.0	33.0
4	30.645033719233123	33.0	28.0	35.0	19.0	37.0
5	31.87023649607093	33.0	32.0	35.0	26.0	37.0
6	31.076088455022223	35.0	30.0	35.0	17.0	37.0
7	31.931859690619344	33.0	31.0	35.0	26.0	35.0
8	32.91436374387739	35.0	32.0	35.0	28.0	37.0
9	34.104257886335596	35.0	32.0	37.0	27.0	39.0
10	33.601494378988455	35.0	32.0	37.0	27.0	39.0
11	33.773828770783275	35.0	32.0	39.0	26.0	39.0
12	33.29183799728605	35.0	31.0	37.0	25.0	39.0
13	33.29496552879209	35.0	32.0	38.0	24.0	39.0
14	34.02158053706951	36.0	32.0	39.0	24.0	40.0
15	34.93746304214819	37.0	33.0	39.0	27.0	40.0
16	35.07561334367868	36.0	33.0	39.0	27.0	41.0
17	34.595824944826695	36.0	32.0	39.0	27.0	40.0
18	34.43505159572468	36.0	32.0	39.0	27.0	40.0
19	33.80850734143871	36.0	32.0	38.0	25.0	40.0
20	33.65625865198265	35.0	32.0	38.0	25.0	40.0
21	34.21278607798434	35.0	32.0	39.0	27.0	40.0
22	34.54587900725656	36.0	33.0	39.0	27.0	40.0
23	34.88896408636772	36.0	33.0	39.0	28.0	40.0
24	34.61042009185486	36.0	33.0	39.0	27.0	40.0
25	33.837976777651306	35.0	32.0	39.0	24.0	40.0
26	33.74845873652285	35.0	32.0	39.0	24.0	40.0
27	34.19127618097071	35.0	33.0	39.0	25.0	40.0
28	33.998344516109924	35.0	33.0	39.0	24.0	40.0
29	34.06968755447544	36.0	33.0	39.0	24.0	40.0
30	33.50326596322843	35.0	32.0	39.0	23.0	40.0
31	33.4949874044223	35.0	32.0	39.0	23.0	40.0
32	32.97657028856463	35.0	31.0	39.0	20.0	40.0
33	32.73910120783102	35.0	31.0	39.0	18.0	40.0
34	32.66428427381453	35.0	31.0	39.0	16.0	40.0
35	32.604615074360055	35.0	32.0	39.0	15.0	40.0
36	32.36414834867476	35.0	31.0	39.0	12.0	40.0
37	32.27094221301623	35.0	31.0	39.0	11.0	40.0
38	32.07441929886326	35.0	31.0	39.0	10.0	40.0
39	32.02439702445855	35.0	31.0	39.0	10.0	40.0
40	31.718084651852763	35.0	30.0	39.0	10.0	40.0
41	31.647955027350235	35.0	30.0	39.0	10.0	40.0
42	31.885722619287698	35.0	31.0	39.0	10.0	40.0
43	31.9125510288952	35.0	31.0	39.0	10.0	40.0
44	31.79681299412434	35.0	30.0	39.0	10.0	40.0
45	31.831717157216282	35.0	30.0	39.0	10.0	40.0
46	31.491064602609807	35.0	30.0	39.0	10.0	40.0
47	31.51463445480119	35.0	30.0	39.0	10.0	40.0
48	31.421933053735433	35.0	30.0	39.0	10.0	40.0
49	31.565103356655946	35.0	30.0	39.0	10.0	40.0
50	31.441804557195088	35.0	30.0	39.0	10.0	40.0
51	29.298921371921033	33.0	25.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	37.0
10	56.0
11	44.0
12	44.0
13	62.0
14	115.0
15	293.0
16	687.0
17	1527.0
18	3003.0
19	5118.0
20	7489.0
21	9984.0
22	13210.0
23	16679.0
24	20688.0
25	24413.0
26	26990.0
27	27617.0
28	29017.0
29	33474.0
30	40982.0
31	52530.0
32	65511.0
33	79951.0
34	89331.0
35	86840.0
36	96101.0
37	90715.0
38	50283.0
39	4883.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.438900339414076	19.35104575766587	21.564016411280075	20.646037491639976
2	31.346296922941953	32.35838662669806	24.458549668504446	11.836766781855532
3	22.763985876546247	31.862994750988104	34.25917380757877	11.113845564886878
4	20.867983989773144	22.430382515902558	42.902668257207274	13.79896523711702
5	27.272758346065633	24.858691404358492	32.876451681632105	14.99209856794377
6	17.526139669062733	40.11113275887017	32.60801946931088	9.754708102756215
7	65.51329685116254	5.199791725770746	26.40810127505301	2.878810148013704
8	62.07699993961414	13.472539817634722	18.469298350554695	5.981161892196439
9	58.4103252974573	5.830311192233239	19.93587705895824	15.823486451351219
10	41.23658835874666	20.305142254260904	25.24903468084937	13.209234706143064
11	34.75331239197483	20.668140992994104	30.23462752751829	14.343919087512774
12	28.55806555625056	18.54928112349591	34.20311750517552	18.689535815078006
13	20.02224022404291	28.167722279759687	38.946483321541	12.8635541746564
14	15.816080639041846	35.298949855814534	31.540671012169458	17.344298492974165
15	10.908989402852264	22.631250932847514	52.576140295708385	13.883619368591837
16	11.43035858943202	22.424913608351023	41.92008786711467	24.224639935102296
17	12.781976303679322	21.618819422369427	38.011300130228356	27.58790414372289
18	15.591627558280893	23.305521659722295	38.964485142231474	22.13836563976534
19	18.499035535366172	26.28778530891922	33.46299201653433	21.75018713918028
20	22.83690464390006	22.884415778254027	37.9437363348521	16.334943242993816
21	18.363224331169697	29.907404559018058	34.58172541754539	17.14764569226685
22	16.02765899994189	21.668837139351183	35.56373612976806	26.739767730938862
23	15.59026033139301	31.67944454128968	33.19057205912345	19.539723068193858
24	18.93780143080294	22.553888678108077	35.75013472881625	22.758175162272742
25	13.27315256315164	31.006996783598744	34.118463373700706	21.601387279548906
26	14.918610122720008	26.167697213933412	34.84730924051686	24.066383422829727
27	18.142758995498408	31.215840690723024	30.097563032007923	20.54383728177065
28	13.349603333299154	26.210195183031804	40.84954921390151	19.590652269767535
29	17.231388339149746	23.214145329382045	34.519288722998695	25.035177608469517
30	16.564865231306307	31.093359948683418	34.25427457789718	18.087500242113094
31	27.225702954007623	23.738818647607523	26.28379756382956	22.751680834555295
32	26.18535722790191	28.967436073597824	27.065851343699194	17.78135535480107
33	20.119199397508687	28.970626269669552	25.190016053522374	25.720158279299387
34	21.85443818938143	24.02263216241744	28.65673376332619	25.46619588487494
35	18.87616228527417	25.807888671271943	29.889402738327586	25.4265463051263
36	24.317269556756436	22.604248201811803	27.143327534012617	25.935154707419144
37	21.285216061725738	28.057318708563056	31.22484160106826	19.432623628642947
38	23.301192107910662	28.393998329704484	24.99894609594059	23.305863466444265
39	25.41446913428333	23.44589028687838	27.42611562865662	23.713524950181668
40	20.445396945843004	25.583663461658972	33.33891617645886	20.632023416039168
41	16.10194499418359	23.8569698378355	27.677457504879293	32.363627663101624
42	21.187915081537994	21.333866551819607	34.29563319125567	23.182585175386723
43	23.005643228979743	22.08663888917373	26.98700792649788	27.92070995534865
44	20.342740993677715	25.281848126158586	30.39960623865629	23.975804641507416
45	19.01345465193252	25.14068194998456	28.452219408013548	27.39364399006938
46	26.09318334854373	26.585498963755956	27.259313948334775	20.06200373936554
47	17.466209557143817	23.55891437627679	36.74467835417784	22.230197712401544
48	20.869579087809008	23.643226701029636	30.362918983831406	25.124275227329957
49	19.020404721945926	19.09400710274368	37.4016308738061	24.48395730150429
50	20.123415013746325	23.457853522147367	31.841802734225904	24.5769287298804
51	18.356046390008306	24.066953100699678	26.34999413231794	31.227006376974074
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	98.0
1	322.0
2	546.0
3	7348.0
4	14150.0
5	12166.0
6	10182.0
7	10584.5
8	10987.0
9	11392.0
10	11797.0
11	11949.5
12	12102.0
13	11549.0
14	10996.0
15	10461.5
16	9927.0
17	9927.0
18	9927.0
19	9239.5
20	8552.0
21	8464.5
22	8377.0
23	8090.0
24	7803.0
25	7466.5
26	6824.0
27	6518.0
28	7228.5
29	7939.0
30	8427.0
31	8915.0
32	10625.0
33	12335.0
34	14023.5
35	15712.0
36	16532.5
37	17353.0
38	19134.0
39	20915.0
40	28271.0
41	35627.0
42	42104.5
43	48582.0
44	53210.5
45	57839.0
46	63433.0
47	69027.0
48	117289.0
49	165551.0
50	123085.0
51	80619.0
52	73854.0
53	67089.0
54	53959.5
55	40830.0
56	33730.5
57	26631.0
58	24976.0
59	23321.0
60	21186.0
61	19051.0
62	15781.5
63	12512.0
64	9800.5
65	7089.0
66	5683.0
67	4277.0
68	3374.5
69	2472.0
70	2280.5
71	2089.0
72	1654.5
73	1220.0
74	961.5
75	584.0
76	465.0
77	370.5
78	276.0
79	183.0
80	90.0
81	60.0
82	30.0
83	21.0
84	12.0
85	10.0
86	8.0
87	10.0
88	12.0
89	7.5
90	3.0
91	3.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	877689.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.63587978178974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.88063330934722	26.220017516131072
2	8.118387361531077	5.136646531827966
3	2.7379552834174072	2.5985287258232743
4	1.3406574597557903	1.696515129015752
5	0.7978872537486121	1.2620932619506728
6	0.5298140682217031	1.0056680501377648
7	0.3518651695247822	0.7792094941739554
8	0.25440282819495813	0.6438605831138404
9	0.20515282826258616	0.5841171190628398
>10	1.6413409023227004	11.78764765945564
>50	0.7438559761091971	17.248000485349678
>100	0.3888505272264985	16.872201434270924
>500	0.0033109316414744435	0.6939887506039615
>1k	0.0033109316414744435	2.189038136862303
>5k	0.0014715251739886414	3.0137244167180253
>10k+	0.001103643880491481	8.268742705502309
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC	27669	3.15248339673848	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	21879	2.492796423334461	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG	21500	2.4496148407921257	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT	7981	0.9093198160168351	TruSeq Adapter, Index 8 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	6649	0.7575576314617136	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6002	0.6838413150899691	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTC	5263	0.5996429259111143	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT	4289	0.48866967684453144	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTA	3402	0.3876088227151075	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGGACTCT	2895	0.32984348670200947	No Hit
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	2303	0.2623936268997333	TruSeq Adapter, Index 8 (95% over 24bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1295	0.1475465683174792	No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	1248	0.1421915963399336	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGT	1157	0.13182345910681348	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT	1126	0.12829145631311317	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTAT	1094	0.12464551794542258	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2787114798066287E-4	0.0	0.0	0.31446218421331473	0.0
2	2.2787114798066287E-4	0.0	0.0	1.5801724756719067	0.0
3	2.2787114798066287E-4	0.0	0.0	1.8392619709259201	0.0
4	2.2787114798066287E-4	0.0	0.0	3.24511301839262	0.0
5	2.2787114798066287E-4	0.0	0.0	6.940271554047048	0.0
6	2.2787114798066287E-4	0.0	0.0	7.6508877290247455	0.0
7	2.2787114798066287E-4	0.0	0.0	8.684511256265033	0.0
8	2.2787114798066287E-4	0.0	0.0	10.59486902536092	0.0
9	2.2787114798066287E-4	0.0	0.0	11.09664129321434	0.0
10	2.2787114798066287E-4	0.0	0.0	14.752036313546142	0.0
11	2.2787114798066287E-4	0.0	0.0	15.869174616521342	0.0
12	2.2787114798066287E-4	0.0	0.0	20.4843629121477	0.0
13	2.2787114798066287E-4	0.0	0.0	21.479818022101224	0.0
14	2.2787114798066287E-4	0.0	0.0	21.81843454800049	0.0
15	2.2787114798066287E-4	0.0	0.0	22.532810596919866	0.0
16	2.2787114798066287E-4	0.0	0.0	23.218816687915652	0.0
17	2.2787114798066287E-4	0.0	0.0	23.748503171396703	0.0
18	0.0043295518116325945	0.0	0.0	24.763441264502575	0.0
19	0.0043295518116325945	0.0	0.0	25.509947145287224	0.0
20	0.004443487385622925	0.0	0.0	25.984944553252916	0.0
21	0.004443487385622925	0.0	0.0	26.32606766177997	0.0
22	0.004443487385622925	0.0	0.0	26.688382787069223	0.0
23	0.004443487385622925	0.0	0.0	27.01309917294167	0.0
24	0.004443487385622925	0.0	0.0	27.282101063132842	0.0
25	0.004443487385622925	0.0	0.0	27.490261356813175	0.0
26	0.004443487385622925	0.0	0.0	27.69397816310789	0.0
27	0.004443487385622925	0.0	0.0	27.90646800859986	0.0
28	0.004443487385622925	0.0	0.0	28.127844828863072	0.0
29	0.004443487385622925	0.0	0.0	28.332017377453745	0.0
30	0.004443487385622925	0.0	0.0	28.603411914698714	0.0
31	0.004443487385622925	0.0	0.0	28.832308482845292	0.0
32	0.004443487385622925	0.0	0.0	29.026340765350824	0.0
33	0.004443487385622925	0.0	0.0	29.23472893017914	0.0
34	0.004443487385622925	0.0	0.0	29.443572837303417	0.0
35	0.004443487385622925	0.0	0.0	29.6782801197235	0.0
36	0.004443487385622925	0.0	0.0	29.889744545049556	0.0
37	0.004443487385622925	0.0	0.0	30.106791813501136	0.0
38	0.004443487385622925	0.0	0.0	30.305039712244316	0.0
39	0.004443487385622925	0.0	0.0	30.511377036740804	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGAG	135	0.0	45.000004	14
TCACGGA	20	7.032374E-4	45.0	17
CTTCGGA	20	7.032374E-4	45.0	12
GTGCACG	40	6.8121153E-9	45.0	1
CGGAATA	20	7.032374E-4	45.0	31
CTATGCG	25	3.8900493E-5	45.0	1
TCGTTAG	35	1.2114833E-7	45.0	1
GCGAATG	35	1.2114833E-7	45.0	1
CTATCGG	20	7.032374E-4	45.0	27
ACGCAAT	20	7.032374E-4	45.0	23
TAGGTTC	20	7.032374E-4	45.0	36
ATAATAG	20	7.032374E-4	45.0	1
CATGACG	20	7.032374E-4	45.0	1
GACGTAC	20	7.032374E-4	45.0	21
AGCTTAG	110	0.0	45.0	1
TAGCGAG	35	1.2114833E-7	45.0	1
CACGCTA	25	3.8900493E-5	45.0	22
CACGCAT	20	7.032374E-4	45.0	43
CAATTGT	25	3.8900493E-5	45.0	13
TCGATGT	25	3.8900493E-5	45.0	16
>>END_MODULE