SRR3548191_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548191_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	730823
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC	16198	2.216405340280752	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	14972	2.048649262543735	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG	13344	1.8258867058097514	No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	7652	1.0470387494646447	TruSeq Adapter, Index 13 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT	5989	0.8194870714249551	TruSeq Adapter, Index 8 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5313	0.7269886142061758	No Hit
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	4494	0.6149231756526546	TruSeq Adapter, Index 8 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTC	4390	0.6006926437728424	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGGACTCT	3867	0.5291294882618637	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT	3306	0.4523667153332612	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTA	2871	0.3928447791052006	No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	2202	0.3013041461475624	TruSeq Adapter, Index 13 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	1978	0.2706537697910438	TruSeq Adapter, Index 13 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	1448	0.1981327900189239	TruSeq Adapter, Index 8 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT	1407	0.1925226764893825	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC	1277	0.17473451163961726	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGT	1039	0.14216848676081623	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	940	0.12862211506753346	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG	844	0.11548623948616833	No Hit
AGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG	812	0.11110761429237996	TruSeq Adapter, Index 8 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATG	790	0.10809730947165047	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCATG	20	7.031597E-4	45.000004	21
AACGTAG	20	7.031597E-4	45.000004	1
CGGAACC	20	7.031597E-4	45.000004	38
TCGTTAG	20	7.031597E-4	45.000004	1
CGAAATG	20	7.031597E-4	45.000004	13
GAACTGC	20	7.031597E-4	45.000004	25
CGTTGGT	20	7.031597E-4	45.000004	27
GAGTAAG	30	2.1644119E-6	45.000004	1
CAACCGT	30	2.1644119E-6	45.000004	45
CGCGCCT	20	7.031597E-4	45.000004	17
CGTCTCG	20	7.031597E-4	45.000004	42
TTCGTAG	20	7.031597E-4	45.000004	1
CGACACT	20	7.031597E-4	45.000004	16
TATGCAA	20	7.031597E-4	45.000004	16
ACGGGTC	20	7.031597E-4	45.000004	5
GCCGCAA	40	6.8084773E-9	45.000004	32
AGTATAT	30	2.1644119E-6	45.000004	40
TATTGCA	20	7.031597E-4	45.000004	19
TAGCACG	40	6.8084773E-9	45.000004	1
GTTGTGC	30	2.1644119E-6	45.000004	44

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content