##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548191_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	730823
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.14889788635552	30.0	25.0	31.0	16.0	33.0
2	27.58100114528415	30.0	25.0	31.0	16.0	33.0
3	27.43185696126148	30.0	25.0	31.0	16.0	34.0
4	31.983421430360018	35.0	32.0	35.0	19.0	37.0
5	33.06318219322599	35.0	32.0	35.0	28.0	37.0
6	31.71952989985263	35.0	31.0	35.0	23.0	37.0
7	33.6134276014849	35.0	33.0	35.0	30.0	37.0
8	33.1147281899995	35.0	33.0	36.0	27.0	37.0
9	35.8010024315053	37.0	35.0	39.0	30.0	39.0
10	35.15173851944999	37.0	34.0	39.0	30.0	39.0
11	35.50666577269735	37.0	35.0	39.0	30.0	39.0
12	35.514080700798964	37.0	34.0	39.0	30.0	39.0
13	35.35552794589114	37.0	34.0	39.0	30.0	39.0
14	35.93831474926213	38.0	34.0	40.0	29.0	41.0
15	36.41927115047009	38.0	35.0	40.0	31.0	41.0
16	36.11737315328062	37.0	34.0	40.0	31.0	41.0
17	35.69823609820709	37.0	34.0	39.0	30.0	41.0
18	35.77660254261292	37.0	34.0	39.0	30.0	40.0
19	35.302155241419605	37.0	34.0	39.0	29.0	40.0
20	35.35520228564235	37.0	34.0	39.0	29.0	40.0
21	35.66395146293973	37.0	34.0	39.0	30.0	40.0
22	36.149907706790835	38.0	34.0	40.0	31.0	41.0
23	36.28099964013174	38.0	35.0	40.0	31.0	41.0
24	36.23879927150623	38.0	35.0	40.0	31.0	41.0
25	35.539720287949336	37.0	34.0	40.0	29.0	41.0
26	35.71178931150224	37.0	34.0	40.0	30.0	41.0
27	35.798646183822896	38.0	34.0	40.0	30.0	41.0
28	35.637514145011856	38.0	34.0	40.0	29.0	41.0
29	35.71734058725574	38.0	34.0	40.0	30.0	41.0
30	34.97668382084308	37.0	34.0	40.0	26.0	41.0
31	35.19641691627111	37.0	34.0	40.0	27.0	41.0
32	34.81757826450454	37.0	34.0	40.0	25.0	41.0
33	34.49338075019533	37.0	34.0	40.0	24.0	41.0
34	34.57991606722832	38.0	34.0	40.0	24.0	41.0
35	34.52810324798207	38.0	34.0	40.0	23.0	41.0
36	34.2649930284077	38.0	34.0	40.0	21.0	41.0
37	34.3221819783997	38.0	34.0	40.0	21.0	41.0
38	34.113625323778805	37.0	33.0	40.0	21.0	41.0
39	34.138450760307215	37.0	33.0	40.0	21.0	41.0
40	33.82029574876543	37.0	33.0	40.0	18.0	41.0
41	33.71756362347654	37.0	33.0	40.0	18.0	41.0
42	34.06452725215271	37.0	33.0	40.0	21.0	41.0
43	34.072815168652326	37.0	33.0	40.0	20.0	41.0
44	34.07061764613319	37.0	34.0	40.0	20.0	41.0
45	34.19721738368935	38.0	34.0	40.0	20.0	41.0
46	33.82619457789369	37.0	33.0	40.0	20.0	41.0
47	33.761872573796936	37.0	33.0	40.0	20.0	41.0
48	33.84600922521596	37.0	33.0	40.0	20.0	41.0
49	33.99470596847664	37.0	34.0	40.0	20.0	41.0
50	33.82042847584162	37.0	33.0	40.0	20.0	41.0
51	31.808347575267884	35.0	30.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	10.0
10	22.0
11	12.0
12	22.0
13	24.0
14	42.0
15	68.0
16	187.0
17	389.0
18	1008.0
19	2120.0
20	3759.0
21	5630.0
22	7226.0
23	8130.0
24	8940.0
25	11122.0
26	13027.0
27	13548.0
28	13842.0
29	15736.0
30	19982.0
31	27526.0
32	39749.0
33	58381.0
34	76292.0
35	83020.0
36	109280.0
37	121970.0
38	76894.0
39	12860.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.42808860695408	21.731800996958224	23.098889881681337	13.741220514406361
2	28.912198986621934	33.123478598785205	24.648512704170503	13.31580971042236
3	23.8093218193735	33.32133772472952	30.359334613168986	12.510005842727992
4	21.87219066723406	24.45872666842724	39.12246877834989	14.54661388598881
5	27.435918136128723	26.86450754833934	29.474441827911818	16.225132487620122
6	18.28897010630481	40.82479615447242	28.545352294604847	12.340881444617917
7	66.30565814157463	6.513889135946734	22.6656796515709	4.51477307090773
8	60.79693715167694	12.99918037609654	15.564917907619218	10.6389645646073
9	56.12987002324776	7.7267683146261135	19.617472356507662	16.52588930561846
10	39.31731759947347	21.983982441712975	22.922650217631354	15.776049741182202
11	30.46975806727484	22.739705783753386	29.075439607127855	17.715096541843923
12	24.58050718162948	18.680309732999646	33.78684031564415	22.952342769726734
13	21.8427717792133	26.389289882775994	38.03916953899918	13.728768799011526
14	15.807247445687944	34.99055174782403	29.127983109453314	20.07421769703471
15	10.360648200727125	24.640850110081374	47.66639801976675	17.332103669424743
16	10.41141288656761	25.64779707261539	37.36965038046148	26.57113966035552
17	11.616082143008635	26.68046845816292	33.56654073558167	28.13690866324678
18	14.267202865810189	24.862654842554218	36.048947556385066	24.821194735250533
19	16.500849042791483	29.484704230709763	30.691289135673074	23.323157590825687
20	20.943237965964396	26.06581894658488	34.19199997810687	18.79894310934385
21	17.04188291829896	33.61196897196722	31.27446727867076	18.071680831063063
22	14.09055270564829	24.76071497476133	32.005971350108034	29.14276096948235
23	14.960530798839116	33.10377478541316	29.638366608604272	22.297327807143454
24	18.50283858061391	25.12373036973385	30.8756018899241	25.49782915972814
25	12.989328469410513	32.83079487098791	28.16974835220019	26.01012830740138
26	14.453294436546196	27.820142496883648	32.07671351339517	25.64984955317498
27	19.805069079654032	31.379827947396294	26.242742770821387	22.572360202128287
28	14.539909116160821	27.754736783051438	34.78119873074602	22.92415537004172
29	18.02857873931171	24.693667276481445	28.983762142132914	28.293991842073936
30	19.013085247727563	32.790292587945366	27.43564447205411	20.76097769227296
31	25.749600108370974	26.81456385472269	20.945290446523988	26.490545590382347
32	25.48811408507942	29.766988723671805	21.797890870976964	22.947006320271804
33	20.051640410879244	30.60946357736415	20.781365665831537	28.557530345925073
34	22.335640777589102	23.395678570597806	25.243868898488415	29.024811753324677
35	19.635397353394733	25.193651540797156	25.870559629349376	29.30039147645873
36	22.476851440088776	25.079123125572128	24.54315203544497	27.90087339889412
37	22.74914719432749	26.234943344694955	28.167559039603297	22.84835042137426
38	24.75702050975407	26.959058486117705	24.214755145910843	24.069165858217378
39	25.905725462936992	22.31374765162016	24.552730278056384	27.227796607386466
40	20.975393494731282	23.842845668513444	31.78704009041861	23.394720746336663
41	16.65670073328289	22.06320819131308	25.991655982365085	35.28843509303894
42	22.797312071459164	19.417560750003762	32.04633680111327	25.738790377423808
43	23.787018197292642	20.435317443484948	26.844803734967293	28.93286062425512
44	21.779555377977978	23.445895928289065	27.779913877915718	26.994634815817236
45	21.3267781665328	20.689688200836592	27.77197761975198	30.21155601287863
46	27.542236629115397	23.590801055796	26.64995491384371	22.2170074012449
47	16.766987355351432	21.426665553766096	35.72068749888824	26.085659591994233
48	19.613504227425793	20.98538223345461	30.402163040845732	28.998950498273864
49	20.90957728478715	15.967751425447748	36.369134523680835	26.753536766084263
50	20.109520362659634	18.44126416382626	33.066556471265955	28.38265900224815
51	17.85753869267935	18.966015026894336	26.756683902942303	36.41976237748402
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1153.0
1	1161.0
2	1169.0
3	5012.5
4	8856.0
5	6392.0
6	3928.0
7	4194.5
8	4461.0
9	4687.0
10	4913.0
11	5195.5
12	5478.0
13	5428.5
14	5379.0
15	5291.0
16	5203.0
17	5420.0
18	5637.0
19	5397.5
20	5158.0
21	5244.0
22	5330.0
23	5162.0
24	4994.0
25	4880.5
26	5003.5
27	5240.0
28	6011.5
29	6783.0
30	7017.0
31	7251.0
32	8125.0
33	8999.0
34	9717.5
35	10436.0
36	11023.5
37	11611.0
38	12772.0
39	13933.0
40	18515.5
41	23098.0
42	27135.5
43	31173.0
44	35607.0
45	40041.0
46	47623.0
47	55205.0
48	96421.0
49	137637.0
50	120501.0
51	103365.0
52	92980.5
53	82596.0
54	64805.5
55	47015.0
56	37156.5
57	27298.0
58	23003.0
59	18708.0
60	16208.0
61	13708.0
62	11056.0
63	8404.0
64	6553.0
65	4702.0
66	3716.0
67	2730.0
68	2196.0
69	1662.0
70	1462.5
71	1263.0
72	984.5
73	706.0
74	530.0
75	299.5
76	245.0
77	197.5
78	150.0
79	103.0
80	56.0
81	36.0
82	16.0
83	12.0
84	8.0
85	5.0
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	730823.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.79309664138793
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.73467937839177	22.563706218249553
2	9.933163156937843	5.918793797786463
3	4.093440095636334	3.658687690950767
4	2.2074967478850276	2.6307265578115273
5	1.3880464591894464	2.0677101150683748
6	0.8987362053164973	1.6065680772065198
7	0.6489475873727467	1.3533910730054244
8	0.5163989559837662	1.230809920090894
9	0.3620939955041897	0.9709111261190349
>10	3.2887084332040635	22.355069993977324
>50	0.8027188313546436	16.09029595768086
>100	0.11208298861816965	5.69108052414039
>500	0.005580895698830024	1.14977202028868
>1k	0.005115821057260855	3.9183421260235702
>5k	0.001395223924707506	2.626268844607332
>10k+	0.001395223924707506	6.167865956993288
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC	16198	2.216405340280752	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	14972	2.048649262543735	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG	13344	1.8258867058097514	No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	7652	1.0470387494646447	TruSeq Adapter, Index 13 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT	5989	0.8194870714249551	TruSeq Adapter, Index 8 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5313	0.7269886142061758	No Hit
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	4494	0.6149231756526546	TruSeq Adapter, Index 8 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTC	4390	0.6006926437728424	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGGACTCT	3867	0.5291294882618637	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT	3306	0.4523667153332612	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTA	2871	0.3928447791052006	No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	2202	0.3013041461475624	TruSeq Adapter, Index 13 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	1978	0.2706537697910438	TruSeq Adapter, Index 13 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	1448	0.1981327900189239	TruSeq Adapter, Index 8 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT	1407	0.1925226764893825	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC	1277	0.17473451163961726	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGT	1039	0.14216848676081623	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	940	0.12862211506753346	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG	844	0.11548623948616833	No Hit
AGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG	812	0.11110761429237996	TruSeq Adapter, Index 8 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATG	790	0.10809730947165047	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3683203730588666E-4	0.0	0.0	0.6818340418952332	0.0
2	1.3683203730588666E-4	0.0	0.0	2.7291149840659092	0.0
3	1.3683203730588666E-4	0.0	0.0	3.4214850928336955	0.0
4	1.3683203730588666E-4	0.0	0.0	5.45918779239296	0.0
5	1.3683203730588666E-4	0.0	0.0	10.091225919271835	0.0
6	1.3683203730588666E-4	0.0	0.0	12.019326156949083	0.0
7	1.3683203730588666E-4	0.0	0.0	13.831940155140163	0.0
8	1.3683203730588666E-4	0.0	0.0	17.788712177914487	0.0
9	1.3683203730588666E-4	0.0	0.0	18.90964022752431	0.0
10	1.3683203730588666E-4	0.0	0.0	23.3280835441687	0.0
11	1.3683203730588666E-4	0.0	0.0	26.250542196947826	0.0
12	2.736640746117733E-4	0.0	0.0	31.47342106091352	0.0
13	2.736640746117733E-4	0.0	0.0	32.69396283368203	0.0
14	2.736640746117733E-4	0.0	0.0	33.1256679113821	0.0
15	2.736640746117733E-4	0.0	0.0	34.103195985895354	0.0
16	2.736640746117733E-4	0.0	0.0	35.3084125704856	0.0
17	2.736640746117733E-4	0.0	0.0	36.45219157032551	0.0
18	0.005883777604153126	0.0	0.0	38.44679217813342	0.0
19	0.005883777604153126	0.0	0.0	39.90720051229915	0.0
20	0.005883777604153126	0.0	0.0	40.83095359615119	0.0
21	0.005883777604153126	0.0	0.0	41.56067885110348	0.0
22	0.005883777604153126	0.0	0.0	42.262216706370765	0.0
23	0.005883777604153126	0.0	0.0	42.96348089756343	0.0
24	0.005883777604153126	0.0	0.0	43.49438920231027	0.0
25	0.005883777604153126	0.0	0.0	43.89859104051186	0.0
26	0.005883777604153126	0.0	0.0	44.270090021797344	0.0
27	0.005883777604153126	0.0	0.0	44.652672398104606	0.0
28	0.005883777604153126	0.0	0.0	45.04852748203053	0.0
29	0.005883777604153126	0.0	0.0	45.428099553517065	0.0
30	0.005883777604153126	0.0	0.0	45.90824317242342	0.0
31	0.006020609641459012	0.0	0.0	46.30574024079702	0.0
32	0.006020609641459012	0.0	0.0	46.636736939039956	0.0
33	0.006020609641459012	0.0	0.0	46.994142220482935	0.0
34	0.006020609641459012	0.0	0.0	47.31679216445021	0.0
35	0.006020609641459012	0.0	0.0	47.688427977773	0.0
36	0.006020609641459012	0.0	0.0	48.02462429343357	0.0
37	0.006020609641459012	0.0	0.0	48.36177843335527	0.0
38	0.006020609641459012	0.0	0.0	48.68429154528525	0.0
39	0.006020609641459012	0.0	0.0	49.01775122019969	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCATG	20	7.031597E-4	45.000004	21
AACGTAG	20	7.031597E-4	45.000004	1
CGGAACC	20	7.031597E-4	45.000004	38
TCGTTAG	20	7.031597E-4	45.000004	1
CGAAATG	20	7.031597E-4	45.000004	13
GAACTGC	20	7.031597E-4	45.000004	25
CGTTGGT	20	7.031597E-4	45.000004	27
GAGTAAG	30	2.1644119E-6	45.000004	1
CAACCGT	30	2.1644119E-6	45.000004	45
CGCGCCT	20	7.031597E-4	45.000004	17
CGTCTCG	20	7.031597E-4	45.000004	42
TTCGTAG	20	7.031597E-4	45.000004	1
CGACACT	20	7.031597E-4	45.000004	16
TATGCAA	20	7.031597E-4	45.000004	16
ACGGGTC	20	7.031597E-4	45.000004	5
GCCGCAA	40	6.8084773E-9	45.000004	32
AGTATAT	30	2.1644119E-6	45.000004	40
TATTGCA	20	7.031597E-4	45.000004	19
TAGCACG	40	6.8084773E-9	45.000004	1
GTTGTGC	30	2.1644119E-6	45.000004	44
>>END_MODULE