SRR3548188_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548188_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	792979
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5522	0.6963614421062853	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC	2035	0.2566272246806031	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG	1936	0.24414265699343865	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	1663	0.20971551579550027	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1532	0.1931955322902624	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	1076	0.13569085688271693	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	895	0.11286553616173946	TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	812	0.10239867638361166	Illumina PCR Primer Index 8 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTATA	30	2.1646465E-6	45.000004	28
GTCGATA	30	2.1646465E-6	45.000004	9
CGCGAAG	30	2.1646465E-6	45.000004	19
GCGTTCC	30	2.1646465E-6	45.000004	40
AGAGTAT	30	2.1646465E-6	45.000004	20
TCCACGT	30	2.1646465E-6	45.000004	36
CGTGTCA	30	2.1646465E-6	45.000004	31
TTACCGA	30	2.1646465E-6	45.000004	14
GCGTCCA	30	2.1646465E-6	45.000004	45
AGTACGG	60	0.0	45.000004	2
ATCGATA	30	2.1646465E-6	45.000004	10
ACTATCA	20	7.031963E-4	45.0	28
ATCTCGA	25	3.8897077E-5	45.0	30
ATTAGTA	20	7.031963E-4	45.0	21
CTCCGTA	20	7.031963E-4	45.0	40
ATAGGTA	25	3.8897077E-5	45.0	24
GTCGAAG	50	2.1827873E-11	45.0	1
CAACGTT	20	7.031963E-4	45.0	27
CTCCGAC	20	7.031963E-4	45.0	29
TCCGATC	25	3.8897077E-5	45.0	15