##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548188_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	792979
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.54858451484844	28.0	25.0	31.0	16.0	33.0
2	27.370859757950715	30.0	25.0	31.0	16.0	33.0
3	27.277587426653167	30.0	25.0	31.0	16.0	33.0
4	30.292025387809765	33.0	28.0	35.0	19.0	37.0
5	31.71842003382183	33.0	30.0	35.0	25.0	37.0
6	30.69845103085958	33.0	28.0	35.0	17.0	37.0
7	31.63876470877539	33.0	30.0	35.0	25.0	35.0
8	33.440793514077924	35.0	33.0	35.0	30.0	37.0
9	34.47205159279123	37.0	33.0	39.0	28.0	39.0
10	33.79208528851331	35.0	32.0	39.0	27.0	39.0
11	33.776349688957716	37.0	32.0	39.0	25.0	39.0
12	33.111889469960744	35.0	31.0	38.0	23.0	39.0
13	33.402006862729024	35.0	32.0	39.0	23.0	39.0
14	33.988076607325034	37.0	32.0	39.0	22.0	40.0
15	35.332424944418456	37.0	33.0	40.0	27.0	41.0
16	35.28502015816308	37.0	33.0	40.0	27.0	41.0
17	34.947045255927335	36.0	33.0	39.0	27.0	40.0
18	34.72453368878621	36.0	33.0	39.0	27.0	40.0
19	34.089564793014695	36.0	32.0	39.0	25.0	40.0
20	34.446572986169876	35.0	33.0	39.0	27.0	40.0
21	34.3358752249429	35.0	33.0	39.0	27.0	40.0
22	34.665330355532745	35.0	33.0	39.0	27.0	40.0
23	35.14725358426894	36.0	33.0	39.0	29.0	40.0
24	34.7060855331604	35.0	33.0	39.0	27.0	40.0
25	33.764599062522464	35.0	32.0	39.0	24.0	40.0
26	33.89267307204857	35.0	32.0	39.0	24.0	40.0
27	34.40982043660677	35.0	33.0	39.0	25.0	40.0
28	34.37326083036247	35.0	33.0	39.0	25.0	40.0
29	34.49645072568126	36.0	33.0	39.0	25.0	40.0
30	33.92781271635188	35.0	33.0	39.0	23.0	40.0
31	33.78739916189458	35.0	33.0	39.0	23.0	40.0
32	33.23121419356629	35.0	32.0	39.0	21.0	40.0
33	33.19759791873429	35.0	32.0	39.0	18.0	40.0
34	32.86519062926004	35.0	32.0	39.0	16.0	40.0
35	32.73093486712763	35.0	32.0	39.0	15.0	40.0
36	32.41561504150803	35.0	31.0	39.0	12.0	40.0
37	32.405985530512154	35.0	31.0	39.0	12.0	40.0
38	32.367422088100696	35.0	31.0	39.0	12.0	40.0
39	32.13743869635892	35.0	31.0	39.0	10.0	40.0
40	32.04943636590629	35.0	31.0	39.0	10.0	40.0
41	31.85884746002101	35.0	31.0	39.0	10.0	40.0
42	31.87854028921321	35.0	31.0	39.0	10.0	40.0
43	31.799905167728276	35.0	31.0	39.0	10.0	40.0
44	31.67434194348148	35.0	30.0	39.0	10.0	40.0
45	31.67170126825553	35.0	30.0	39.0	10.0	40.0
46	31.44356660138541	35.0	30.0	38.0	10.0	40.0
47	31.587620857551084	35.0	30.0	39.0	10.0	40.0
48	31.416961861537317	35.0	30.0	38.0	10.0	40.0
49	31.408488749386805	35.0	30.0	38.0	10.0	40.0
50	31.290480580191907	35.0	30.0	38.0	10.0	40.0
51	29.530959836263005	34.0	26.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	16.0
9	58.0
10	61.0
11	39.0
12	45.0
13	67.0
14	111.0
15	265.0
16	623.0
17	1422.0
18	2576.0
19	4109.0
20	6301.0
21	8818.0
22	11465.0
23	14968.0
24	19000.0
25	23194.0
26	25155.0
27	25384.0
28	25478.0
29	28715.0
30	34888.0
31	44376.0
32	57031.0
33	71048.0
34	79475.0
35	75065.0
36	85979.0
37	86574.0
38	54374.0
39	6297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.193432612969577	23.353581872912148	25.055014067207328	23.397971446910955
2	36.202598051146374	21.79628968736877	28.853727526201826	13.147384735283026
3	27.40022119122953	21.177357786271767	38.13215734590701	13.290263676591687
4	24.75349284155066	26.689105260038414	34.242899244494495	14.314502653916433
5	20.122979297055785	30.330563608872367	35.56361517770332	13.98284191636853
6	19.36104234790581	36.45506375326459	33.72409609838344	10.459797800446166
7	76.59711039006078	5.334945818237305	16.264239027767445	1.8037047639344799
8	77.09964576615522	4.448919832681572	15.74972351096309	2.701710890200119
9	72.53962589173231	5.560676890560784	17.359349995397103	4.540347222309796
10	41.071201128907575	26.58531940946734	22.093018856741477	10.25046060488361
11	35.47370106900687	22.499965320645313	29.55172835598421	12.47460525436361
12	32.3427228211592	21.23587131563383	32.31434880368837	14.1070570595186
13	20.501047316511535	31.56212207385063	32.7877535218461	15.149077087791731
14	15.676455492516194	34.50015700289667	34.16433474278638	15.659052761800755
15	13.665052920695251	22.633638469618994	49.68618336677264	14.015125242913115
16	15.817568939404447	20.564100688668933	46.50375356724453	17.11457680468209
17	16.90107808655715	20.143282482890466	34.33861426342942	28.61702516712296
18	19.77681628391168	23.362661558502808	38.67731680157986	18.183205356005644
19	24.82562589929872	23.249165488619497	33.887782652504036	18.037425959577742
20	26.95191171519044	22.946761515752623	33.53354880772378	16.56777796133315
21	20.583647234037723	25.80724079704507	35.71342998994929	17.895681978967918
22	19.66344632077268	24.352599501373934	34.093336645737146	21.890617532116234
23	18.10564970825205	28.01171279441196	33.49508625070777	20.38755124662822
24	19.467602546851808	22.95949829692842	40.3237664553538	17.249132700865974
25	16.45730845331339	25.012516094373243	38.222449774836406	20.307725677476956
26	15.978102824917178	29.69864271311094	34.68011132703388	19.643143134937997
27	18.001989964425288	30.258556657868617	33.549816577740394	18.1896367999657
28	15.241261117885847	28.018270345116324	39.7091221835635	17.03134635343433
29	16.909653345170554	25.275700869758218	37.572117294405025	20.24252849066621
30	19.132536927207404	25.793495161914752	36.19704935439652	18.87691855648132
31	24.863331815848845	26.52340099800877	30.54015301792355	18.073114168218833
32	26.59200306691602	25.558432190511983	31.22415599908699	16.62540874348501
33	23.89836300835205	27.490639726903233	29.4962413884857	19.114755876259018
34	21.97864003964796	26.535759458951624	31.861121164621004	19.62447933677941
35	20.92716200555122	26.68116053514658	30.56335665887747	21.828320800424727
36	26.711300047037817	26.625169140670813	29.174038656761404	17.48949215552997
37	23.046259737016996	29.600909986266977	30.13963799797977	17.21319227873626
38	22.549525271161027	29.636850408396693	26.882048578840045	20.931575741602238
39	23.325712282418575	26.9490112600712	29.575688637404017	20.149587820106206
40	23.625215800166206	23.630386176683118	30.638137958256145	22.10626006489453
41	18.026076352589413	25.64279760245858	31.1875850432357	25.14354100171631
42	23.601633838979343	25.884544231310038	29.03633009196965	21.477491837740974
43	22.836796434710124	26.07168663987319	29.55261110319441	21.53890582222228
44	21.122627459239148	28.3317717114829	28.79193522148758	21.75366560779037
45	18.96922869331975	30.594000597745968	27.57071750954313	22.866053199391157
46	22.515476450196033	29.051589008031737	28.61790791433317	19.81502662743906
47	21.30005964849006	26.63777981510229	30.412659099421298	21.649501436986352
48	21.520494237552317	23.975035908895443	32.6163744563223	21.88809539722994
49	21.32288496921104	24.244021594518898	32.02556435920749	22.40752907706257
50	19.13795951721294	28.559898811948365	29.78855682180739	22.51358484903131
51	19.02585062151709	27.359362606071535	27.28975168320977	26.325035089201602
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	72.0
1	334.0
2	596.0
3	7357.0
4	14118.0
5	11849.5
6	9581.0
7	10080.5
8	10580.0
9	11193.0
10	11806.0
11	11727.0
12	11648.0
13	11068.0
14	10488.0
15	9984.5
16	9481.0
17	8904.0
18	8327.0
19	8405.0
20	8483.0
21	7640.5
22	6798.0
23	6830.5
24	6863.0
25	6809.5
26	7041.0
27	7326.0
28	7900.0
29	8474.0
30	10033.5
31	11593.0
32	14164.5
33	16736.0
34	17269.5
35	17803.0
36	18840.0
37	19877.0
38	22798.5
39	25720.0
40	32359.5
41	38999.0
42	47964.0
43	56929.0
44	59907.0
45	62885.0
46	62814.5
47	62744.0
48	64958.0
49	67172.0
50	65180.5
51	63189.0
52	58297.0
53	53405.0
54	47684.5
55	41964.0
56	37413.5
57	32863.0
58	30801.5
59	28740.0
60	24594.5
61	20449.0
62	18511.0
63	16573.0
64	13396.5
65	10220.0
66	8053.0
67	5886.0
68	4526.5
69	3167.0
70	2435.0
71	1703.0
72	1556.0
73	1409.0
74	1039.0
75	587.5
76	506.0
77	371.0
78	236.0
79	159.0
80	82.0
81	51.0
82	20.0
83	23.0
84	26.0
85	17.5
86	9.0
87	6.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	792979.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.269179627408086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.02479660754389	27.11411254885264
2	7.783842903647491	5.023564496986532
3	2.4650870588620744	2.3863901129885807
4	1.145529246534988	1.478611560995479
5	0.6659358068757011	1.074460108619747
6	0.4168615838299676	0.8071068797025035
7	0.29301938263034594	0.6618846564687598
8	0.19972924029262934	0.5156078985478887
9	0.1633306746328131	0.47434921905527916
>10	1.430032671606015	10.68274258196753
>50	0.7926926323090934	18.97012588838085
>100	0.6135822228723995	28.402517532275546
>500	0.0031771247786273114	0.6446146840080945
>1k	0.0019857029866420695	1.0562453728816532
>5k	3.9714059732841393E-4	0.7076664582689262
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5522	0.6963614421062853	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC	2035	0.2566272246806031	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG	1936	0.24414265699343865	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	1663	0.20971551579550027	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1532	0.1931955322902624	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	1076	0.13569085688271693	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	895	0.11286553616173946	TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	812	0.10239867638361166	Illumina PCR Primer Index 8 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12320628919555247	0.0
2	0.0	0.0	0.0	0.5466727366046263	0.0
3	0.0	0.0	0.0	0.7135119593330971	0.0
4	0.0	0.0	0.0	1.0338230898926706	0.0
5	0.0	0.0	0.0	1.8464549502571947	0.0
6	0.0	0.0	0.0	2.3150676121309646	0.0
7	0.0	0.0	0.0	2.6970449406604713	0.0
8	0.0	0.0	0.0	3.6400711746464913	0.0
9	0.0	0.0	0.0	4.004519665716242	0.0
10	0.0	0.0	0.0	4.946915365980688	0.0
11	0.0	0.0	0.0	5.7677441647256735	0.0
12	0.0	0.0	0.0	6.765752939232943	0.0
13	0.0	0.0	0.0	7.155927206142912	0.0
14	0.0	0.0	0.0	7.319361546774883	0.0
15	0.0	0.0	0.0	7.524663326519366	0.0
16	0.0	0.0	0.0	7.901722492020596	0.0
17	0.0	0.0	0.0	8.278655550777511	0.0
18	6.305337215739635E-4	0.0	0.0	9.019910993859863	0.0
19	6.305337215739635E-4	0.0	0.0	9.287635612040168	0.0
20	6.305337215739635E-4	0.0	0.0	9.619674669821016	0.0
21	6.305337215739635E-4	0.0	0.0	9.881093950785582	0.0
22	6.305337215739635E-4	0.0	0.0	10.134064079881057	0.0
23	6.305337215739635E-4	0.0	0.0	10.38779084944242	0.0
24	6.305337215739635E-4	0.0	0.0	10.58514790429507	0.0
25	6.305337215739635E-4	0.0	0.0	10.733701649097895	0.0
26	6.305337215739635E-4	0.0	0.0	10.88225539390072	0.0
27	6.305337215739635E-4	0.0	0.0	11.045689734532692	0.0
28	6.305337215739635E-4	0.0	0.0	11.20534087283522	0.0
29	6.305337215739635E-4	0.0	0.0	11.374450016961356	0.0
30	6.305337215739635E-4	0.0	0.0	11.583282785546654	0.0
31	6.305337215739635E-4	0.0	0.0	11.77130794132001	0.0
32	6.305337215739635E-4	0.0	0.0	11.936381669628073	0.0
33	6.305337215739635E-4	0.0	0.0	12.097924409095322	0.0
34	6.305337215739635E-4	0.0	0.0	12.273969424158773	0.0
35	6.305337215739635E-4	0.0	0.0	12.463255647375277	0.0
36	6.305337215739635E-4	0.0	0.0	12.649263095239597	0.0
37	6.305337215739635E-4	0.0	0.0	12.839936492643563	0.0
38	6.305337215739635E-4	0.0	0.0	13.021277990968235	0.0
39	6.305337215739635E-4	0.0	0.0	13.251548906087047	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTATA	30	2.1646465E-6	45.000004	28
GTCGATA	30	2.1646465E-6	45.000004	9
CGCGAAG	30	2.1646465E-6	45.000004	19
GCGTTCC	30	2.1646465E-6	45.000004	40
AGAGTAT	30	2.1646465E-6	45.000004	20
TCCACGT	30	2.1646465E-6	45.000004	36
CGTGTCA	30	2.1646465E-6	45.000004	31
TTACCGA	30	2.1646465E-6	45.000004	14
GCGTCCA	30	2.1646465E-6	45.000004	45
AGTACGG	60	0.0	45.000004	2
ATCGATA	30	2.1646465E-6	45.000004	10
ACTATCA	20	7.031963E-4	45.0	28
ATCTCGA	25	3.8897077E-5	45.0	30
ATTAGTA	20	7.031963E-4	45.0	21
CTCCGTA	20	7.031963E-4	45.0	40
ATAGGTA	25	3.8897077E-5	45.0	24
GTCGAAG	50	2.1827873E-11	45.0	1
CAACGTT	20	7.031963E-4	45.0	27
CTCCGAC	20	7.031963E-4	45.0	29
TCCGATC	25	3.8897077E-5	45.0	15
>>END_MODULE