##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548186_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	662688
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.647481167608287	28.0	25.0	31.0	16.0	33.0
2	27.423636764208798	30.0	25.0	31.0	16.0	33.0
3	27.30918924139263	30.0	25.0	31.0	16.0	33.0
4	30.242035467671062	33.0	28.0	35.0	19.0	37.0
5	31.766884265295282	33.0	32.0	35.0	25.0	37.0
6	30.73192663817664	33.0	28.0	35.0	17.0	37.0
7	31.730736032642813	33.0	30.0	35.0	25.0	35.0
8	33.338872893427975	35.0	33.0	35.0	29.0	37.0
9	34.400259247187215	37.0	33.0	38.0	28.0	39.0
10	33.81232344632768	35.0	32.0	39.0	27.0	39.0
11	33.929627517021586	37.0	32.0	39.0	26.0	39.0
12	33.358821949393985	35.0	31.0	38.0	25.0	39.0
13	33.598622579554785	35.0	32.0	39.0	25.0	39.0
14	34.25746052440968	37.0	32.0	39.0	24.0	40.0
15	35.45205586942875	37.0	33.0	40.0	27.0	41.0
16	35.40723538075233	37.0	34.0	40.0	29.0	41.0
17	35.0321071152639	36.0	33.0	39.0	27.0	40.0
18	34.84042867835241	36.0	33.0	39.0	27.0	40.0
19	34.27963536385147	36.0	32.0	39.0	26.0	40.0
20	34.39532178038534	35.0	32.0	39.0	27.0	40.0
21	34.41102449418128	35.0	33.0	39.0	27.0	40.0
22	34.738659821816604	36.0	33.0	39.0	27.0	40.0
23	35.17226809599691	36.0	33.0	39.0	29.0	40.0
24	34.80699363803178	36.0	33.0	39.0	27.0	40.0
25	33.8812714278816	35.0	32.0	39.0	24.0	40.0
26	34.00439573373895	35.0	33.0	39.0	24.0	40.0
27	34.5352624462794	36.0	33.0	39.0	26.0	40.0
28	34.464212419720894	36.0	33.0	39.0	25.0	40.0
29	34.59633190883191	36.0	33.0	39.0	25.0	40.0
30	33.999447703896855	36.0	33.0	39.0	23.0	40.0
31	33.93400514269158	36.0	33.0	39.0	23.0	40.0
32	33.40524952919021	36.0	32.0	39.0	21.0	40.0
33	33.385979525810036	36.0	33.0	39.0	19.0	40.0
34	33.06331033608576	36.0	32.0	39.0	17.0	40.0
35	32.980005673861605	36.0	32.0	40.0	15.0	41.0
36	32.64665423245932	36.0	32.0	40.0	12.0	41.0
37	32.61608932106814	36.0	32.0	40.0	12.0	41.0
38	32.55095610604085	36.0	31.0	40.0	11.0	40.0
39	32.34979356801391	36.0	31.0	39.0	10.0	40.0
40	32.31159157854073	36.0	31.0	39.0	10.0	40.0
41	32.165293471437536	36.0	31.0	39.0	10.0	40.0
42	32.21630088367377	36.0	31.0	39.0	10.0	40.0
43	32.16763393935004	36.0	31.0	39.0	10.0	40.0
44	32.071525363368586	35.0	31.0	39.0	10.0	40.0
45	32.08966210343329	35.0	31.0	39.0	10.0	40.0
46	31.843983895890677	35.0	31.0	39.0	10.0	40.0
47	32.000202206770005	35.0	31.0	39.0	10.0	40.0
48	31.833959570718044	35.0	31.0	39.0	10.0	40.0
49	31.897888599159785	35.0	31.0	39.0	10.0	40.0
50	31.823662115505336	35.0	31.0	39.0	10.0	40.0
51	30.089524783910377	34.0	27.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	20.0
9	34.0
10	56.0
11	51.0
12	36.0
13	48.0
14	95.0
15	251.0
16	558.0
17	1237.0
18	2155.0
19	3471.0
20	5246.0
21	7058.0
22	9535.0
23	12202.0
24	15189.0
25	18790.0
26	20035.0
27	19758.0
28	20318.0
29	22570.0
30	27727.0
31	35594.0
32	44680.0
33	56346.0
34	64667.0
35	64356.0
36	75553.0
37	78850.0
38	50535.0
39	5666.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.507551064754455	20.887808440774542	26.08044811434642	23.524192380124585
2	35.49000434593655	24.451325510647546	28.13194142643295	11.926728716982954
3	25.670753054227628	23.642649331208652	38.34036530011106	12.346232314452653
4	23.807130957554687	25.59862257955478	37.184919600173835	13.409326862716695
5	21.381856922111158	29.888122313969774	35.28749577478391	13.442524989135157
6	18.61962190352021	37.84842942681926	33.29304287990729	10.238905789753247
7	74.86766019605002	4.3681189337969	18.987970206190546	1.7762506639625284
8	74.21094089526294	6.3532159930465015	16.21773745714424	3.2181056545463327
9	69.80992563619682	5.562346081413878	17.932420686657974	6.695307595731324
10	36.97622410546139	27.104761214930704	23.891333478197886	12.027681201410015
11	31.992732628325847	22.072981553913756	32.697136510695834	13.237149307064561
12	29.45458496305954	19.60590805929789	34.93287942440485	16.006627553237724
13	19.13540006760346	30.020311217345114	37.04850548070887	13.795783234342556
14	14.922557825100197	34.497078564875174	34.198597228258244	16.381766381766383
15	13.117937853107344	22.401643005456563	50.177609976338786	14.3028091650973
16	14.804855376889275	21.469077454246946	44.9982495533343	18.727817615529478
17	14.58182432758704	21.912725143657347	37.0537870008209	26.451663527934716
18	18.137796368728573	23.640084021439954	39.19566975711044	19.02644985272104
19	21.72983364720653	25.055229610314356	35.04001883239171	18.1749179100874
20	24.47863851465546	23.552712588729538	35.62551306195374	16.343135834661258
21	18.897278960838282	27.826820464532332	36.8894562750495	16.386444299579892
22	18.50599377082428	22.953637307450865	35.189410401274806	23.350958520450046
23	16.978578154425612	28.88719880245304	34.89470157902361	19.239521464097738
24	18.11878289632527	23.13471799700613	39.254370080641266	19.49212902602733
25	15.143476266357622	27.201186682118884	37.94349678883577	19.711840262687723
26	15.362131198995604	29.86714713409629	35.33231324544884	19.43840842145927
27	17.43278888406007	29.949991549567823	35.06174851513835	17.555471051233763
28	14.394707615046599	26.819559128881167	41.97480564005988	16.81092761601236
29	16.73774083731711	23.957578830460186	38.69649065623642	20.608189675986285
30	16.961073687768604	27.013919069003816	37.29568062195181	18.729326621275774
31	22.844536191993818	27.532866145154284	31.102117436863196	18.5204802259887
32	24.187400405620746	27.7347711140084	30.925412863972184	17.152415616398667
33	21.212546477376986	27.152898498237484	31.448283355063015	20.186271669322515
34	18.472041141532667	28.392697619392536	32.12024361388768	21.015017625187117
35	17.989913805591772	26.665187841035298	33.85016780143899	21.49473055193394
36	24.071508764305374	26.17883528900478	31.54999034236322	18.19966560432662
37	20.378820802549615	28.988151286880097	32.959703510550966	17.673324400019315
38	20.84359457240813	30.56189337968999	28.980606258148633	19.613905789753247
39	22.470000965763678	27.54493819112463	30.772701482447246	19.212359360664447
40	23.485712733594088	25.716928630064224	30.871843159978752	19.925515476362936
41	16.597252402337148	27.582814235356608	31.809841131875032	24.010092230431212
42	20.932927712588732	26.973477715003142	30.768023564633733	21.325571007774396
43	22.06709643150321	25.10638490511372	31.848471678980157	20.97804698440292
44	20.653459848375103	28.210409725240233	30.432269786083342	20.70386064030132
45	19.179764836544496	29.885255203051813	28.164083248829012	22.77089671157468
46	23.25408035153798	28.587962962962965	29.385321599304646	18.77263508619441
47	19.330363610024627	26.66745134965474	33.556062581486316	20.446122458834324
48	20.716385387995555	26.049815297696654	31.297986382732145	21.935812931575644
49	19.379557197353808	24.599208073784347	34.30271862475252	21.718516104109327
50	19.12453522623014	27.09102926263943	31.003881162779468	22.78055434835096
51	17.499034236322373	28.028574532811824	28.784888212854316	25.687503018011494
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	62.0
1	232.5
2	403.0
3	6173.5
4	11944.0
5	10032.5
6	8121.0
7	8504.5
8	8888.0
9	9266.5
10	9645.0
11	9581.5
12	9518.0
13	9189.5
14	8861.0
15	8579.5
16	8298.0
17	7804.0
18	7310.0
19	7108.0
20	6906.0
21	6669.5
22	6433.0
23	6322.5
24	6212.0
25	6211.0
26	6503.5
27	6797.0
28	8408.5
29	10020.0
30	10937.0
31	11854.0
32	14034.5
33	16215.0
34	16428.0
35	16641.0
36	17984.5
37	19328.0
38	23015.0
39	26702.0
40	32476.5
41	38251.0
42	44865.5
43	51480.0
44	67003.5
45	82527.0
46	72321.0
47	62115.0
48	57101.5
49	52088.0
50	46102.0
51	40116.0
52	37226.5
53	34337.0
54	29272.0
55	24207.0
56	21851.5
57	19496.0
58	18264.0
59	17032.0
60	15261.0
61	13490.0
62	11418.5
63	9347.0
64	7389.5
65	5432.0
66	4052.5
67	2673.0
68	2043.0
69	1413.0
70	1124.5
71	836.0
72	769.0
73	702.0
74	532.0
75	248.0
76	134.0
77	135.0
78	136.0
79	124.5
80	113.0
81	64.5
82	16.0
83	11.0
84	6.0
85	5.0
86	4.0
87	4.0
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	662688.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.13847231482475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.45283437381288	27.654994414902532
2	7.759392211456154	5.142688079584131
3	2.532059688144609	2.517257696251918
4	1.1744582040661973	1.5567900272146593
5	0.6680250375396769	1.1068664606059175
6	0.4303784629822666	0.8557250868260803
7	0.299222229913931	0.6941037307388049
8	0.21445897489545035	0.5685474241790863
9	0.18106022194556254	0.5400053237033144
>10	1.9110146878048528	16.328289000174045
>50	1.0646064404478979	25.224638874649575
>100	0.3059695675302166	12.891190530553601
>500	0.003259949730154515	0.8092971847843683
>1k	0.0018628284172311515	1.4570127234075558
>5k	0.0013971213129233635	2.6525934424244326
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGC	6310	0.9521826259114395	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCG	5610	0.8465522236708677	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5268	0.7949442271476169	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC	4849	0.7317168863779033	No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	1850	0.27916606306436814	No Hit
GAACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT	1569	0.23676300159351008	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTC	1173	0.17700637404027234	No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT	1000	0.1509005746293882	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCT	929	0.14018663383070162	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAGGAATT	763	0.11513713844222319	No Hit
GTGCACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	704	0.10623400453908928	No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	678	0.10231058959872519	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17730817518953113	0.0
2	0.0	0.0	0.0	0.7664240185426626	0.0
3	0.0	0.0	0.0	0.9586713506205031	0.0
4	0.0	0.0	0.0	1.4987445072190835	0.0
5	0.0	0.0	0.0	2.995074605244097	0.0
6	0.0	0.0	0.0	3.575589115843353	0.0
7	0.0	0.0	0.0	4.1213964942778505	0.0
8	0.0	0.0	0.0	5.406616688396349	0.0
9	0.0	0.0	0.0	5.861431020329325	0.0
10	0.0	0.0	0.0	7.576265150417693	0.0
11	0.0	0.0	0.0	8.517130233231928	0.0
12	0.0	0.0	0.0	10.411837848278527	0.0
13	0.0	0.0	0.0	10.91192235260032	0.0
14	0.0	0.0	0.0	11.07700758124487	0.0
15	0.0	0.0	0.0	11.360700661548119	0.0
16	0.0	0.0	0.0	11.82698343715293	0.0
17	0.0	0.0	0.0	12.250561350137621	0.0
18	0.002565309768699599	0.0	0.0	13.141780143898789	0.0
19	0.002565309768699599	0.0	0.0	13.5505698005698	0.0
20	0.002565309768699599	0.0	0.0	13.917861799217732	0.0
21	0.002565309768699599	0.0	0.0	14.217399439857067	0.0
22	0.002565309768699599	0.0	0.0	14.494000193152736	0.0
23	0.002565309768699599	0.0	0.0	14.78403109759042	0.0
24	0.002565309768699599	0.0	0.0	14.999969819885074	0.0
25	0.002565309768699599	0.0	0.0	15.168827562895359	0.0
26	0.002565309768699599	0.0	0.0	15.344777632913226	0.0
27	0.002565309768699599	0.0	0.0	15.50880655753537	0.0
28	0.002565309768699599	0.0	0.0	15.689887247090637	0.0
29	0.002565309768699599	0.0	0.0	15.873231445265343	0.0
30	0.002565309768699599	0.0	0.0	16.106825534791636	0.0
31	0.002565309768699599	0.0	0.0	16.30299628180984	0.0
32	0.002565309768699599	0.0	0.0	16.49343280699213	0.0
33	0.002565309768699599	0.0	0.0	16.687189144816262	0.0
34	0.002565309768699599	0.0	0.0	16.877022067700032	0.0
35	0.002565309768699599	0.0	0.0	17.083906755516924	0.0
36	0.002565309768699599	0.0	0.0	17.26830725771404	0.0
37	0.002565309768699599	0.0	0.0	17.456782075426144	0.0
38	0.002565309768699599	0.0	0.0	17.65416002704138	0.0
39	0.002565309768699599	0.0	0.0	17.856970399343282	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAG	30	2.1641044E-6	45.000004	1
TCTTGCG	30	2.1641044E-6	45.000004	1
TAGCGCA	30	2.1641044E-6	45.000004	13
TTCACGG	30	2.1641044E-6	45.000004	2
TAAACGG	60	0.0	45.000004	2
ATGCGCC	30	2.1641044E-6	45.000004	37
TCGACCG	30	2.1641044E-6	45.000004	19
TCACGAC	20	7.031122E-4	45.0	25
AACCGTC	20	7.031122E-4	45.0	33
CGGGTCC	45	3.8380676E-10	45.0	6
CTATGCG	20	7.031122E-4	45.0	1
GGCAGTC	35	1.210883E-7	45.0	27
AACCGGA	25	3.889011E-5	45.0	26
AACCGAT	25	3.889011E-5	45.0	35
ACCGGTC	20	7.031122E-4	45.0	17
CTCGTCC	20	7.031122E-4	45.0	37
ATTAGCG	40	6.8066583E-9	45.0	1
ACTTGGC	40	6.8066583E-9	45.0	28
GCTATCG	35	1.210883E-7	45.0	1
CCGTGTC	20	7.031122E-4	45.0	31
>>END_MODULE