FastQCFastQC Report
Sat 18 Jun 2016
SRR3548184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241619
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGC66982.772132986230387No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG58192.408337092695525No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTC52502.1728423675290434No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20240.8376824670245303No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC18570.7685653860002732No Hit
GAACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT16780.6944818081359496No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTC14730.6096374871181488No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCT11630.48133631874976723No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAATCACGT9060.3749705114250121No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTA8950.3704178893216179No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT8200.339377284071203No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC4850.2007292472860164No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAATCACGTCGT4460.18458813255580064No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTAT2840.11754042521490446No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT2780.11505717679487126No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA351.2066084E-745.0000045
CTCCGCC302.158362E-645.00000445
GTTGTAG351.2066084E-745.0000041
CGGGATA351.2066084E-745.0000046
TAGATAA302.158362E-645.00000428
AGTAGAG351.2066084E-745.0000041
TCACGGA207.022218E-445.018
CGGGTCC253.8816324E-545.06
GATACAA207.022218E-445.09
CGAACCG207.022218E-445.029
CGTATGG207.022218E-445.045
TGCCAGG207.022218E-445.02
CACGGAT207.022218E-445.019
GGCACGG207.022218E-445.02
GGACTAC207.022218E-445.08
CCAATTC207.022218E-445.023
GTAGGGT453.8198777E-1045.04
AGGGTAC253.8816324E-545.06
CTAAGCG207.022218E-445.01
TGAGTAG207.022218E-445.01