##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548184_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	241619
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.779694477669388	28.0	25.0	31.0	16.0	33.0
2	27.499406089752874	30.0	25.0	31.0	16.0	33.0
3	27.399951990530546	30.0	25.0	31.0	16.0	33.0
4	30.264242464375734	33.0	28.0	35.0	19.0	37.0
5	31.87618109502978	33.0	32.0	35.0	26.0	37.0
6	31.14437606314073	35.0	30.0	35.0	17.0	36.0
7	32.041114316341016	35.0	31.0	35.0	26.0	35.0
8	32.88088685078574	35.0	32.0	35.0	28.0	37.0
9	34.17398466180226	35.0	32.0	37.0	27.0	39.0
10	33.95886912866952	35.0	32.0	39.0	27.0	39.0
11	34.24592850727799	37.0	32.0	39.0	27.0	39.0
12	33.7258162644494	35.0	32.0	38.0	25.0	39.0
13	33.85984959792069	35.0	32.0	39.0	25.0	39.0
14	34.64436985501968	37.0	33.0	39.0	25.0	40.0
15	35.350721590603385	37.0	33.0	40.0	27.0	41.0
16	35.35677243925353	37.0	33.0	40.0	27.0	41.0
17	34.78716491666632	36.0	33.0	39.0	27.0	40.0
18	34.647163509492216	36.0	33.0	39.0	27.0	40.0
19	34.013587507604946	36.0	32.0	38.0	26.0	40.0
20	33.78041048096383	35.0	32.0	38.0	27.0	40.0
21	34.31229746005074	35.0	33.0	39.0	27.0	40.0
22	34.61416941548471	36.0	33.0	39.0	27.0	40.0
23	34.929670265997295	36.0	33.0	39.0	28.0	40.0
24	34.63886532102194	36.0	33.0	39.0	27.0	40.0
25	33.810139103298994	35.0	32.0	39.0	24.0	40.0
26	33.772199206188255	35.0	32.0	39.0	24.0	40.0
27	34.26375409218646	35.0	33.0	39.0	25.0	40.0
28	34.080275143924936	35.0	33.0	39.0	24.0	40.0
29	34.05554198966141	36.0	33.0	39.0	24.0	40.0
30	33.476994772762076	35.0	32.0	39.0	22.0	40.0
31	33.336927145630106	35.0	32.0	39.0	21.0	40.0
32	32.82298163637793	35.0	31.0	39.0	18.0	40.0
33	32.44594589001693	35.0	31.0	39.0	15.0	40.0
34	32.10071227842181	35.0	31.0	39.0	12.0	40.0
35	31.943758562033615	36.0	31.0	39.0	10.0	40.0
36	31.509794345643346	35.0	30.0	39.0	9.0	40.0
37	31.43202728262264	35.0	30.0	39.0	9.0	40.0
38	31.29338752333219	35.0	30.0	39.0	8.0	40.0
39	31.03287820908124	35.0	29.0	39.0	8.0	40.0
40	30.898352364673308	35.0	27.0	39.0	8.0	40.0
41	30.44666189331137	35.0	26.0	39.0	8.0	40.0
42	30.673912233723343	35.0	27.0	39.0	8.0	40.0
43	30.491865292050708	35.0	27.0	39.0	8.0	40.0
44	30.412744858641084	35.0	27.0	39.0	7.0	40.0
45	30.543620327871565	35.0	27.0	39.0	8.0	40.0
46	30.233367409019987	35.0	26.0	38.0	7.0	40.0
47	30.214440917311965	35.0	26.0	38.0	8.0	40.0
48	30.152032745769166	35.0	26.0	38.0	7.0	40.0
49	30.222184513635103	35.0	26.0	38.0	7.0	40.0
50	30.03468270293313	35.0	26.0	38.0	7.0	40.0
51	27.85213911157649	32.0	21.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	11.0
10	14.0
11	11.0
12	15.0
13	13.0
14	43.0
15	95.0
16	273.0
17	595.0
18	1153.0
19	1814.0
20	2709.0
21	3626.0
22	4492.0
23	5639.0
24	6727.0
25	8060.0
26	8349.0
27	8100.0
28	8091.0
29	8898.0
30	10538.0
31	12970.0
32	16330.0
33	19880.0
34	22952.0
35	23541.0
36	26549.0
37	25137.0
38	13634.0
39	1355.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.253850069737894	19.791903782401217	20.979310401913757	23.974935745947132
2	34.33794527748232	32.40018376038309	22.018135990960978	11.243734971173625
3	21.75532553317413	31.55091280073173	36.49257715659778	10.201184509496356
4	19.916066203402877	22.657986333856194	44.61817986168306	12.807767601057865
5	24.875527172945837	25.81005632835166	35.1421038908364	14.172312607866102
6	15.958595971343314	39.815577417338865	34.706707667857245	9.519118943460573
7	61.87551475670373	5.994147811223455	28.967506694423868	3.1628307376489433
8	58.40103634234063	14.062635802647971	21.790918760527937	5.745409094483463
9	54.56855628075606	7.1546525728523	22.905069551649497	15.371721594742136
10	34.234890468050935	25.041077067614715	27.97793219904064	12.746100265293705
11	28.34338359152219	21.562459905884886	35.7285643927009	14.36559210989202
12	24.303138412128185	18.061493508374753	38.75233321882799	18.88303486066907
13	18.784118798604414	25.78356834520464	43.18907039595396	12.243242460236985
14	15.151540234832526	32.93449604542689	35.103199665589216	16.81076405415137
15	11.07901282597809	22.189066257206594	52.3336327027262	14.398288214089124
16	11.708516300456504	24.094131670108727	40.356511698169435	23.84084033126534
17	12.424105720162736	22.748211026450736	40.1367442129965	24.690939040390035
18	13.786581353287614	23.339638025155306	41.64200663027328	21.2317739912838
19	16.751579966807245	25.00093121815751	37.47304640777423	20.774442407261017
20	18.871032493305577	24.281616925821233	41.20909365571416	15.63825692515903
21	16.75985746154069	29.84202401301222	38.01108356544808	15.387034959999008
22	13.55067275338446	22.81691423273832	38.49655863156457	25.13585438231265
23	14.366419859365365	29.75345481936437	37.60838344666603	18.271741874604235
24	17.534630968591046	22.984119626353888	38.04336579490851	21.437883610146553
25	12.977456243093465	31.15773180089314	36.1469917514765	19.717820204536896
26	14.79519408655776	26.04720655246483	37.646459922439874	21.511139438537533
27	16.197401694403172	30.94417243677029	33.94145327975035	18.916972589076188
28	12.23289559182018	27.40099081611959	42.57860515936247	17.78750843269776
29	18.341686705101836	22.746555527504047	38.11413837487946	20.79761939251466
30	14.868863789685413	30.793522032621606	36.16644386410009	18.17117031359289
31	20.849353734598687	28.98902818073082	30.237274386534175	19.924343698136322
32	22.16920026984633	27.848803281198915	31.821173003778675	18.16082344517608
33	18.306507352484697	27.057060909944997	28.60909117246574	26.027340565104566
34	21.41429275015624	27.83762866330876	31.36301367028255	19.385064916252446
35	17.345076339195177	26.295117519731477	30.181401297083426	26.178404843989917
36	26.439973677566748	26.07079741245515	28.091333876888818	19.397895033089284
37	18.300713106171287	29.416974658449874	34.9426162677604	17.33969596761844
38	19.14998406582264	29.87016749510593	26.43128230809663	24.5485661309748
39	21.919633803632994	26.52150700069117	32.06867009630865	19.490189099367186
40	20.424304380036336	22.657572459119525	30.414412773829874	26.503710387014266
41	20.324146693761666	28.47623738199397	26.551719856468242	24.647896067776127
42	21.84223922787529	22.876098320082445	31.353080676602417	23.928581775439845
43	25.361002239062323	22.79870374432474	29.09415236384556	22.746141652767374
44	20.118450949635584	25.202488214916873	31.396123649216328	23.282937186231216
45	16.572372205828184	24.682247670919917	31.886565212172886	26.858814911079016
46	24.474068678373804	25.123438140212485	30.179331923400067	20.22316125801365
47	16.642730911062458	23.233686092567226	38.33266423584238	21.790918760527937
48	19.395411784669253	24.340801013165354	30.88416059995282	25.379626602212575
49	19.27497423629764	20.15156092856936	37.81366531605544	22.759799519077557
50	20.915159817729567	20.792239020937924	33.768453639821374	24.52414752151114
51	17.71963297588352	22.364549145555607	29.703375976227036	30.212441902333843
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	43.0
1	105.0
2	167.0
3	2734.0
4	5301.0
5	4497.5
6	3694.0
7	3749.5
8	3805.0
9	3966.5
10	4128.0
11	4089.5
12	4051.0
13	4033.0
14	4015.0
15	3914.0
16	3813.0
17	3776.0
18	3739.0
19	3481.5
20	3224.0
21	3107.0
22	2990.0
23	2825.5
24	2661.0
25	2610.5
26	2485.0
27	2410.0
28	2472.0
29	2534.0
30	2946.0
31	3358.0
32	4423.0
33	5488.0
34	4933.0
35	4378.0
36	4620.5
37	4863.0
38	5677.0
39	6491.0
40	7956.5
41	9422.0
42	11465.5
43	13509.0
44	15607.5
45	17706.0
46	30600.5
47	43495.0
48	33357.5
49	23220.0
50	21042.0
51	18864.0
52	15108.5
53	11353.0
54	9837.5
55	8322.0
56	7437.5
57	6553.0
58	5822.0
59	5091.0
60	4290.5
61	3490.0
62	3128.5
63	2767.0
64	2241.0
65	1715.0
66	1299.0
67	883.0
68	707.0
69	531.0
70	477.0
71	423.0
72	321.5
73	220.0
74	197.0
75	126.0
76	78.0
77	67.0
78	56.0
79	40.0
80	24.0
81	15.5
82	7.0
83	4.5
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	241619.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.05247113484016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.58075399112202	35.98357998630958
2	7.037134013440968	6.059320179713384
3	2.130308690314733	2.751451601942226
4	1.0854323213501529	1.8692217473500004
5	0.6586158739324552	1.417752045071228
6	0.507701134445583	1.3114673061506439
7	0.39676624350645323	1.195723707208838
8	0.3328039124812965	1.146242466853031
9	0.3587886009165282	1.3902062296021667
>10	3.818749982077322	30.935638333854403
>50	0.05996466865320246	1.7086069128977082
>100	0.0219870451728409	1.9323479339772367
>500	0.002998233432660123	1.1277408004797012
>1k	0.004997055721100205	3.526072437974495
>5k	0.002998233432660123	7.6446283106153565
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGC	6698	2.772132986230387	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG	5819	2.408337092695525	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTC	5250	2.1728423675290434	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2024	0.8376824670245303	No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	1857	0.7685653860002732	No Hit
GAACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT	1678	0.6944818081359496	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTC	1473	0.6096374871181488	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCT	1163	0.48133631874976723	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAATCACGT	906	0.3749705114250121	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTA	895	0.3704178893216179	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	820	0.339377284071203	No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	485	0.2007292472860164	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAATCACGTCGT	446	0.18458813255580064	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTAT	284	0.11754042521490446	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT	278	0.11505717679487126	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.40518336720208264	0.0
2	0.0	0.0	0.0	1.7970441066306873	0.0
3	0.0	0.0	0.0	2.204710722252803	0.0
4	0.0	0.0	0.0	3.4513014291094657	0.0
5	0.0	0.0	0.0	7.029662402377297	0.0
6	0.0	0.0	0.0	7.9194930862225235	0.0
7	0.0	0.0	0.0	9.00301714683034	0.0
8	0.0	0.0	0.0	11.03390047968082	0.0
9	0.0	0.0	0.0	11.61373898575857	0.0
10	0.0	0.0	0.0	15.278185904254219	0.0
11	0.0	0.0	0.0	16.619140051072144	0.0
12	0.0	0.0	0.0	20.987587896647202	0.0
13	0.0	0.0	0.0	21.994131256233988	0.0
14	0.0	0.0	0.0	22.34302765924865	0.0
15	0.0	0.0	0.0	23.11283466945894	0.0
16	0.0	0.0	0.0	24.000182104884136	0.0
17	0.0	0.0	0.0	24.714943775117025	0.0
18	0.0012416242100165963	0.0	0.0	26.23510568291401	0.0
19	0.0012416242100165963	0.0	0.0	27.113761748869088	0.0
20	0.0012416242100165963	0.0	0.0	27.67870076442664	0.0
21	0.0012416242100165963	0.0	0.0	28.03835791059478	0.0
22	0.0012416242100165963	0.0	0.0	28.43029728622335	0.0
23	0.0012416242100165963	0.0	0.0	28.797404177651593	0.0
24	0.0012416242100165963	0.0	0.0	29.073044752275276	0.0
25	0.0012416242100165963	0.0	0.0	29.291156738501524	0.0
26	0.0012416242100165963	0.0	0.0	29.505957726834396	0.0
27	0.0012416242100165963	0.0	0.0	29.774976305671327	0.0
28	0.0012416242100165963	0.0	0.0	29.968255807697243	0.0
29	0.0012416242100165963	0.0	0.0	30.201681159180364	0.0
30	0.0012416242100165963	0.0	0.0	30.467802614860588	0.0
31	0.0012416242100165963	0.0	0.0	30.69791696845033	0.0
32	0.0012416242100165963	0.0	0.0	30.932170069406794	0.0
33	0.0012416242100165963	0.0	0.0	31.14572943352965	0.0
34	0.0024832484200331927	0.0	0.0	31.35721942396914	0.0
35	0.0024832484200331927	0.0	0.0	31.600577769132393	0.0
36	0.0024832484200331927	0.0	0.0	31.814137133255247	0.0
37	0.0024832484200331927	0.0	0.0	32.04963185842173	0.0
38	0.0024832484200331927	0.0	0.0	32.24580848360435	0.0
39	0.0024832484200331927	0.0	0.0	32.475508962457425	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGTA	35	1.2066084E-7	45.000004	5
CTCCGCC	30	2.158362E-6	45.000004	45
GTTGTAG	35	1.2066084E-7	45.000004	1
CGGGATA	35	1.2066084E-7	45.000004	6
TAGATAA	30	2.158362E-6	45.000004	28
AGTAGAG	35	1.2066084E-7	45.000004	1
TCACGGA	20	7.022218E-4	45.0	18
CGGGTCC	25	3.8816324E-5	45.0	6
GATACAA	20	7.022218E-4	45.0	9
CGAACCG	20	7.022218E-4	45.0	29
CGTATGG	20	7.022218E-4	45.0	45
TGCCAGG	20	7.022218E-4	45.0	2
CACGGAT	20	7.022218E-4	45.0	19
GGCACGG	20	7.022218E-4	45.0	2
GGACTAC	20	7.022218E-4	45.0	8
CCAATTC	20	7.022218E-4	45.0	23
GTAGGGT	45	3.8198777E-10	45.0	4
AGGGTAC	25	3.8816324E-5	45.0	6
CTAAGCG	20	7.022218E-4	45.0	1
TGAGTAG	20	7.022218E-4	45.0	1
>>END_MODULE