SRR3548180_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548180_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	509218
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGC	19285	3.7871795576747087	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC	16920	3.3227419297825294	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCG	14840	2.914272472693424	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT	6302	1.2375839031613178	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	4416	0.8672120781276389	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTC	4203	0.8253832346853409	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3258	0.6398045630751465	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCT	3028	0.5946372673393321	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTA	2516	0.4940909394404754	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTGAAGGT	2049	0.40238169114210415	No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	1592	0.31263623831050746	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGT	1095	0.21503560361181262	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	892	0.1751705556362894	Illumina Single End Adapter 1 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	777	0.1525869077683821	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTAT	770	0.15121225094163993	No Hit
GCGATTGGGTCATTATTGGGGTATCTAACTTTAAGTTTGGAGCTATTTTTG	745	0.14630276227470357	No Hit
TTGCTAGAATGTTCCTCCCCAGAAGATCAATTTGTCAATCAATAAAGACCC	740	0.1453208645413163	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT	731	0.14355344862121922	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATG	682	0.13393085083402392	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTGC	45	3.8380676E-10	45.000004	10
TCACGAG	35	1.2101373E-7	45.0	10
GTGCACG	25	3.887734E-5	45.0	1
GTGCACA	25	3.887734E-5	45.0	10
AGCGTAT	20	7.0295826E-4	45.0	22
AGACTCC	20	7.0295826E-4	45.0	15
CGTGGAG	25	3.887734E-5	45.0	21
GACAGGT	20	7.0295826E-4	45.0	44
GTCGCTA	25	3.887734E-5	45.0	22
AGCCGGT	25	3.887734E-5	45.0	45
ATTAGTG	20	7.0295826E-4	45.0	11
GTCGATG	20	7.0295826E-4	45.0	1
CTGTACG	20	7.0295826E-4	45.0	1
CACGGTC	25	3.887734E-5	45.0	32
CCTAGTC	20	7.0295826E-4	45.0	40
CGAAACT	20	7.0295826E-4	45.0	14
TTAGCGT	20	7.0295826E-4	45.0	20
TAGACAA	25	3.887734E-5	45.0	1
ATGGATG	20	7.0295826E-4	45.0	12
GACGTTG	25	3.887734E-5	45.0	23

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content