FastQCFastQC Report
Sat 18 Jun 2016
SRR3548178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778112
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42290.5434950238526073No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC39290.5049401628557328No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA14670.18853327027471622No Hit
TTTTGTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA10210.13121504359269617No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGC9220.1184919394637276No Hit
TTTTTTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA8470.10885322421450896No Hit
TTTTCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA8220.10564031913143608No Hit
CTTTTTGGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAG8070.10371257608159236No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATC207.031879E-445.00000422
CGGAATT207.031879E-445.00000428
AGCGTCC207.031879E-445.00000416
AGGTAAT207.031879E-445.00000432
AGCCGCG207.031879E-445.00000428
TCGTTAA551.8189894E-1245.00000419
ACGCCCG207.031879E-445.00000440
ATCTAAC207.031879E-445.00000435
TACCAGT207.031879E-445.00000435
ACGTTGT207.031879E-445.00000415
ACGTTCG406.8102963E-945.00000439
TAGGTTA207.031879E-445.00000443
TAGGTCC207.031879E-445.00000420
CGGTTAT207.031879E-445.00000432
GGCGTAG207.031879E-445.00000413
GGCCGGA406.8102963E-945.0000048
TCTTACC207.031879E-445.00000441
ATAGCGC207.031879E-445.00000444
GAATCGA207.031879E-445.00000444
GAATAGA406.8102963E-945.00000443