##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548178_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	778112
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.728181804161867	30.0	25.0	31.0	16.0	33.0
2	28.501251747820366	31.0	28.0	33.0	16.0	34.0
3	26.54284344670176	31.0	23.0	33.0	10.0	34.0
4	30.286280124198058	33.0	28.0	35.0	19.0	37.0
5	32.403895583155126	35.0	32.0	35.0	27.0	37.0
6	33.664287403355814	35.0	33.0	37.0	28.0	37.0
7	33.39284961547952	35.0	32.0	35.0	28.0	37.0
8	33.57332877529199	35.0	33.0	37.0	28.0	37.0
9	35.32316041906564	37.0	35.0	39.0	30.0	39.0
10	35.6830443432308	37.0	35.0	39.0	30.0	39.0
11	35.70751511350551	37.0	35.0	39.0	30.0	39.0
12	34.33893963850962	35.0	33.0	39.0	26.0	39.0
13	34.114576050748475	35.0	33.0	39.0	25.0	39.0
14	34.859062705625924	37.0	33.0	40.0	25.0	41.0
15	35.75682935104458	37.0	33.0	40.0	30.0	41.0
16	35.47933459450567	37.0	34.0	40.0	29.0	41.0
17	35.49636684693206	36.0	34.0	40.0	30.0	41.0
18	35.235516223885504	36.0	34.0	39.0	29.0	40.0
19	34.54836707312058	36.0	33.0	39.0	27.0	40.0
20	34.83493764393815	35.0	34.0	39.0	29.0	40.0
21	34.84025564443165	35.0	34.0	39.0	29.0	40.0
22	35.00118877488074	35.0	33.0	39.0	29.0	41.0
23	35.289534154466196	35.0	34.0	39.0	30.0	41.0
24	35.16627426385919	35.0	34.0	40.0	29.0	41.0
25	34.45504631723968	35.0	33.0	39.0	27.0	41.0
26	34.45576343765422	35.0	33.0	39.0	26.0	40.0
27	34.72442270521467	35.0	33.0	39.0	28.0	41.0
28	34.64162228573779	35.0	33.0	39.0	27.0	41.0
29	34.75280936420464	35.0	34.0	40.0	27.0	41.0
30	34.362832085869385	35.0	33.0	39.0	25.0	41.0
31	34.134915025086364	35.0	33.0	39.0	24.0	41.0
32	33.71275214879092	35.0	33.0	39.0	23.0	41.0
33	33.52917446331634	35.0	33.0	40.0	21.0	41.0
34	33.25265128927455	35.0	33.0	40.0	18.0	41.0
35	33.02422144884027	35.0	33.0	40.0	17.0	41.0
36	32.72906599564073	35.0	32.0	40.0	15.0	41.0
37	32.56874974296759	35.0	32.0	40.0	15.0	41.0
38	32.52579962781707	35.0	32.0	40.0	15.0	41.0
39	32.366980588912654	35.0	31.0	39.0	13.0	41.0
40	32.22722435844711	35.0	31.0	39.0	12.0	41.0
41	32.21213912650107	35.0	31.0	39.0	12.0	41.0
42	32.219441417173876	35.0	31.0	39.0	12.0	41.0
43	32.1805575546965	35.0	31.0	39.0	12.0	41.0
44	32.03033110914624	35.0	31.0	39.0	10.0	40.0
45	31.987631600592202	35.0	31.0	39.0	10.0	40.0
46	31.869295165734496	35.0	31.0	39.0	10.0	40.0
47	31.945013057246257	35.0	31.0	39.0	11.0	40.0
48	31.86335129133081	35.0	31.0	39.0	10.0	40.0
49	31.869852926056918	35.0	31.0	39.0	10.0	40.0
50	31.741942034051654	35.0	31.0	38.0	10.0	40.0
51	30.299037927701924	34.0	28.0	37.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	37.0
9	106.0
10	161.0
11	131.0
12	128.0
13	163.0
14	179.0
15	321.0
16	554.0
17	990.0
18	1839.0
19	2964.0
20	4372.0
21	6268.0
22	8807.0
23	11942.0
24	16482.0
25	21083.0
26	24151.0
27	24457.0
28	24397.0
29	25685.0
30	29669.0
31	36938.0
32	48384.0
33	65972.0
34	91179.0
35	74847.0
36	65046.0
37	76469.0
38	80046.0
39	34342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.729725283763777	3.8232285326533972	49.40445591380161	30.042590269781215
2	25.983020439216975	4.64251932883698	52.47972014311565	16.8947400888304
3	21.631975859516366	3.354401422931403	54.305035778911005	20.708586938641226
4	17.42564052475736	3.5502601167955254	57.37824374897187	21.64585560947524
5	15.586445139003125	4.885800501727258	58.993178359927626	20.534575999341996
6	18.27963069583813	6.586069871689422	60.05343703734167	15.08086239513078
7	78.46338316334923	2.387959573943083	16.85734187366343	2.2913153890442506
8	81.47220451554531	2.7048805313373907	14.598412567856556	1.2245023852607335
9	78.38961486264188	3.4146755222898504	14.755973433130448	3.4397361819378185
10	49.030859310741896	19.778900723803257	20.795335375884193	10.394904589570652
11	42.07800419476887	19.365592613916764	26.45364677578549	12.10275641552887
12	38.13872553051489	17.074791289685802	31.349728573778584	13.436754606020726
13	20.883240458957065	32.82213871524922	33.51882505346274	12.775795772330975
14	15.295870003290016	35.10651422931404	33.949996915611116	15.647618851784834
15	14.133826492844218	19.29490870208916	51.99187263530186	14.579392169764763
16	16.266295854581344	16.78357357295608	50.60801015792072	16.342120414541867
17	16.199724461260075	15.823943082743872	35.92310618522783	32.05322627076822
18	20.893778787629543	19.936718621483795	40.74182122882053	18.427681362066128
19	26.03545505017272	20.54138735811811	34.43141861325876	18.991738978450403
20	27.325500699128145	19.932606102977463	34.34685495147228	18.39503824642211
21	23.511011268300706	22.518866178647805	37.384977998025995	16.585144555025497
22	23.07778314689916	19.161123334430005	34.51444008060536	23.246653438065472
23	18.73971870373417	25.22888735811811	32.452526114492514	23.578867823655205
24	19.089668325382465	19.319326780720512	43.42858097548939	18.162423918407633
25	17.253300296101333	19.901376665569995	42.10460704885672	20.74071598947195
26	16.656985112683007	28.098140113505508	34.60260219608488	20.6422725777266
27	16.441848988320448	29.660383081098864	34.948567815430174	18.949200115150518
28	14.463599070570817	26.559158578713603	43.08505716400724	15.89218518670834
29	16.944989924329658	20.413128187201842	41.648503043263695	20.993378845204806
30	16.758769945714754	26.452490129955585	37.44717984043428	19.341560083895377
31	23.895403232439545	26.28516203322915	32.19318041618687	17.626254318144433
32	26.10266902451061	23.899387234742555	33.24071598947195	16.75722775127488
33	25.866456242803093	24.45200690903109	30.90352031584142	18.778016532324397
34	19.639589159401215	27.998283023523605	31.443288369797664	20.918839447277513
35	18.462252220759993	25.71724893074519	34.43437448593519	21.38612436255963
36	28.463511679552557	22.810854992597466	29.754328425727916	18.97130490212206
37	20.150441067609805	29.835550666228	32.86994674288534	17.144061523276854
38	21.65240993584471	29.512203898667543	27.789701225530518	21.04568493995723
39	20.903417502878764	28.46364019575588	29.806891552886988	20.826050748478366
40	22.21929490870209	24.716621771672973	30.569249670998524	22.494833648626418
41	17.473191519986838	25.082764434939957	31.17867350715578	26.26537053791742
42	21.45346685310084	25.08854766408949	28.43883656851456	25.019148914295116
43	21.65151032242145	25.882906316828425	29.73106699292647	22.734516367823655
44	19.400163472610625	29.136294003948016	30.25759787794045	21.205944645500903
45	18.858210643197896	33.22786436913966	26.313564114163512	21.600360873498932
46	22.687350921204143	29.48033188024346	28.552573408455338	19.279743790097058
47	20.587910223721007	28.43138262872183	30.370178072051324	20.61052907550584
48	23.094233220924494	24.493132094094424	30.72256950156276	21.690065183418326
49	21.97871257608159	22.397803915117617	33.788966112847504	21.83451739595328
50	20.224980465537097	29.181660223721007	30.443817856555356	20.149541454186544
51	19.596150682678072	30.091554943247246	26.405710231946045	23.90658414212864
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	57.0
1	392.0
2	727.0
3	7250.5
4	13774.0
5	12675.0
6	11576.0
7	11095.0
8	10614.0
9	10851.0
10	11088.0
11	10864.5
12	10641.0
13	10165.0
14	9689.0
15	8989.5
16	8290.0
17	7637.0
18	6984.0
19	6406.5
20	5829.0
21	5469.0
22	5109.0
23	5369.0
24	5629.0
25	6103.0
26	6782.5
27	6988.0
28	8413.0
29	9838.0
30	10565.0
31	11292.0
32	13181.0
33	15070.0
34	14732.0
35	14394.0
36	16414.5
37	18435.0
38	21393.0
39	24351.0
40	32272.0
41	40193.0
42	50538.0
43	60883.0
44	65475.5
45	70068.0
46	68354.0
47	66640.0
48	63178.0
49	59716.0
50	54073.5
51	48431.0
52	45265.5
53	42100.0
54	39518.5
55	36937.0
56	36180.5
57	35424.0
58	33109.5
59	30795.0
60	28016.0
61	25237.0
62	22450.5
63	19664.0
64	15946.5
65	12229.0
66	10563.0
67	8897.0
68	7601.0
69	6305.0
70	4919.5
71	3534.0
72	2608.5
73	1683.0
74	1450.5
75	894.0
76	570.0
77	392.5
78	215.0
79	218.5
80	222.0
81	152.0
82	82.0
83	80.5
84	79.0
85	46.0
86	13.0
87	9.5
88	6.0
89	5.0
90	4.0
91	8.0
92	12.0
93	7.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	778112.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.442226064717033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.98546475618168	22.773058839247458
2	7.61444324649753	4.179145458546862
3	2.6263000815988518	2.1621456165906143
4	1.2006547750970602	1.3179455905558204
5	0.801869667952107	1.100254435119065
6	0.5423446702289317	0.8929887027250052
7	0.3942647937067909	0.7573652518782545
8	0.31026482913072084	0.6811486064748835
9	0.25733795640281326	0.6355733737174468
>10	1.712134012050934	9.913197230210596
>50	0.6704223906721485	13.44592807583879
>100	0.8735082894857961	39.07799477566138
>500	0.009079134299907069	1.6672179755230092
>1k	0.0019113966947172777	1.3960360679108037
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4229	0.5434950238526073	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	3929	0.5049401628557328	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1467	0.18853327027471622	No Hit
TTTTGTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1021	0.13121504359269617	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGC	922	0.1184919394637276	No Hit
TTTTTTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA	847	0.10885322421450896	No Hit
TTTTCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	822	0.10564031913143608	No Hit
CTTTTTGGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAG	807	0.10371257608159236	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.570324066458299E-4	0.0	0.0	0.024803627241322585	0.0
2	2.570324066458299E-4	0.0	0.0	0.0893187613094259	0.0
3	2.570324066458299E-4	0.0	0.0	0.11142354828096726	0.0
4	2.570324066458299E-4	0.0	0.0	0.17593868234907056	0.0
5	2.570324066458299E-4	0.0	0.0	0.3518773646981411	0.0
6	2.570324066458299E-4	0.0	0.0	0.39827171409771345	0.0
7	2.570324066458299E-4	0.0	0.0	0.4522485194933377	0.0
8	2.570324066458299E-4	0.0	0.0	0.539511021549597	0.0
9	2.570324066458299E-4	0.0	0.0	0.584748725119263	0.0
10	2.570324066458299E-4	0.0	0.0	0.7723823819707188	0.0
11	2.570324066458299E-4	0.0	0.0	0.8789223145254154	0.0
12	2.570324066458299E-4	0.0	0.0	1.160629832209245	0.0
13	2.570324066458299E-4	0.0	0.0	1.223859804244119	0.0
14	2.570324066458299E-4	0.0	0.0	1.245193493995723	0.0
15	2.570324066458299E-4	0.0	0.0	1.285933130449087	0.0
16	2.570324066458299E-4	0.0	0.0	1.3397814196413884	0.0
17	2.570324066458299E-4	0.0	0.0	1.4201040467182102	0.0
18	3.855486099687449E-4	0.0	0.0	1.5351260486922191	0.0
19	3.855486099687449E-4	0.0	0.0	1.592958340187531	0.0
20	3.855486099687449E-4	0.0	0.0	1.6640278006251028	0.0
21	3.855486099687449E-4	0.0	0.0	1.736253906892581	0.0
22	3.855486099687449E-4	0.0	0.0	1.8002549761473927	0.0
23	3.855486099687449E-4	0.0	0.0	1.8681115315018917	0.0
24	3.855486099687449E-4	0.0	0.0	1.9139918160881724	0.0
25	3.855486099687449E-4	0.0	0.0	1.9592295196578384	0.0
26	3.855486099687449E-4	0.0	0.0	2.002282447771015	0.0
27	3.855486099687449E-4	0.0	0.0	2.0444357624609313	0.0
28	3.855486099687449E-4	0.0	0.0	2.085689463727587	0.0
29	3.855486099687449E-4	0.0	0.0	2.135553750616878	0.0
30	3.855486099687449E-4	0.0	0.0	2.2009684981082414	0.0
31	3.855486099687449E-4	0.0	0.0	2.2553308521138344	0.0
32	3.855486099687449E-4	0.0	0.0	2.3094361737127818	0.0
33	3.855486099687449E-4	0.0	0.0	2.363027430498437	0.0
34	5.140648132916598E-4	0.0	0.0	2.4209882381970718	0.0
35	5.140648132916598E-4	0.0	0.0	2.485503372265175	0.0
36	5.140648132916598E-4	0.0	0.0	2.543335663760487	0.0
37	5.140648132916598E-4	0.0	0.0	2.603609763118934	0.0
38	5.140648132916598E-4	0.0	0.0	2.6652975407139334	0.0
39	5.140648132916598E-4	0.0	0.0	2.7389373252179636	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGATC	20	7.031879E-4	45.000004	22
CGGAATT	20	7.031879E-4	45.000004	28
AGCGTCC	20	7.031879E-4	45.000004	16
AGGTAAT	20	7.031879E-4	45.000004	32
AGCCGCG	20	7.031879E-4	45.000004	28
TCGTTAA	55	1.8189894E-12	45.000004	19
ACGCCCG	20	7.031879E-4	45.000004	40
ATCTAAC	20	7.031879E-4	45.000004	35
TACCAGT	20	7.031879E-4	45.000004	35
ACGTTGT	20	7.031879E-4	45.000004	15
ACGTTCG	40	6.8102963E-9	45.000004	39
TAGGTTA	20	7.031879E-4	45.000004	43
TAGGTCC	20	7.031879E-4	45.000004	20
CGGTTAT	20	7.031879E-4	45.000004	32
GGCGTAG	20	7.031879E-4	45.000004	13
GGCCGGA	40	6.8102963E-9	45.000004	8
TCTTACC	20	7.031879E-4	45.000004	41
ATAGCGC	20	7.031879E-4	45.000004	44
GAATCGA	20	7.031879E-4	45.000004	44
GAATAGA	40	6.8102963E-9	45.000004	43
>>END_MODULE