FastQCFastQC Report
Sat 18 Jun 2016
SRR3548176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682638
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45270.6631626132737996No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGC25200.36915612667328807No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCG18310.2682241539439645No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTC17530.25679789288026744No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14630.2143156402075478No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA10220.14971331803972238No Hit
TGTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA8800.1289116632827355No Hit
TTGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA8450.12378449485671761No Hit
TTTTCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA7580.11103981905490172No Hit
TTTCCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA7280.1066451032611721No Hit
TTTTTTGGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAG6940.10166442536161187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTCC351.2109558E-745.00000435
GTTGAAT253.889134E-545.00000425
TGTCACG351.2109558E-745.00000428
AACCGTC253.889134E-545.00000411
AACGTAG253.889134E-545.00000420
CTATGCG253.889134E-545.00000423
GATACGA502.1827873E-1145.00000419
CTCACAT253.889134E-545.00000438
GTACATT351.2109558E-745.00000433
AGTCATC453.8380676E-1045.00000412
ATTAGGT253.889134E-545.00000420
AGTCAAT253.889134E-545.00000420
CACGGGT351.2109558E-745.00000423
CAACGAG253.889134E-545.00000435
TAGACCG253.889134E-545.00000425
TCCGAAC351.2109558E-745.00000443
CGCATAC453.8380676E-1045.00000437
TAGAACG253.889134E-545.00000417
TAGCGGA253.889134E-545.00000429
TTCACTC253.889134E-545.00000412