##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548176_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	682638
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.770737931377976	30.0	25.0	31.0	16.0	33.0
2	28.568487250929483	31.0	28.0	33.0	16.0	34.0
3	26.46456980127095	30.0	22.0	33.0	10.0	34.0
4	30.379866049062606	33.0	28.0	35.0	19.0	37.0
5	32.545057556127844	35.0	32.0	35.0	28.0	37.0
6	33.74007160456933	35.0	33.0	37.0	30.0	37.0
7	33.52046472654614	35.0	32.0	35.0	29.0	37.0
8	33.65774685851066	35.0	35.0	37.0	29.0	37.0
9	35.42531619980136	37.0	35.0	39.0	30.0	39.0
10	35.80398395635754	37.0	35.0	39.0	30.0	39.0
11	35.774493948476355	37.0	35.0	39.0	30.0	39.0
12	34.39028152549375	35.0	33.0	39.0	27.0	39.0
13	34.10292424388915	35.0	33.0	39.0	25.0	39.0
14	34.88471488548836	37.0	33.0	40.0	25.0	41.0
15	35.83784524154823	37.0	34.0	40.0	30.0	41.0
16	35.63146645806416	37.0	34.0	40.0	30.0	41.0
17	35.60855533972618	36.0	34.0	40.0	30.0	41.0
18	35.3393350502023	36.0	34.0	39.0	29.0	40.0
19	34.58146924138416	36.0	33.0	39.0	27.0	40.0
20	34.829786797687795	35.0	34.0	39.0	29.0	40.0
21	34.88051207228428	35.0	34.0	39.0	29.0	40.0
22	35.07242198647013	35.0	33.0	39.0	30.0	40.0
23	35.366749287323586	35.0	34.0	39.0	30.0	41.0
24	35.20298606289131	35.0	34.0	39.0	30.0	41.0
25	34.45569247536762	35.0	33.0	39.0	27.0	40.0
26	34.43022949205875	35.0	33.0	39.0	27.0	40.0
27	34.74508158057419	35.0	34.0	39.0	29.0	40.0
28	34.64170907567408	35.0	33.0	39.0	27.0	41.0
29	34.68661135184388	35.0	34.0	39.0	27.0	41.0
30	34.30555872951696	35.0	33.0	39.0	25.0	41.0
31	34.01429161576121	35.0	33.0	39.0	24.0	41.0
32	33.51721263685877	35.0	33.0	39.0	22.0	41.0
33	33.28960151646993	35.0	33.0	39.0	20.0	41.0
34	33.025225668656006	35.0	33.0	40.0	18.0	41.0
35	32.8182199057187	35.0	33.0	40.0	15.0	41.0
36	32.50586548067936	35.0	32.0	40.0	15.0	41.0
37	32.39284364480149	35.0	32.0	40.0	14.0	41.0
38	32.24337057122516	35.0	31.0	39.0	12.0	41.0
39	32.16173872535663	35.0	31.0	39.0	11.0	41.0
40	32.029481218449604	35.0	31.0	39.0	10.0	41.0
41	31.986371986323643	35.0	31.0	39.0	10.0	41.0
42	31.984283031416357	35.0	31.0	39.0	10.0	41.0
43	31.95303660212294	35.0	31.0	39.0	10.0	41.0
44	31.803620659851926	35.0	31.0	39.0	10.0	40.0
45	31.7453496582376	35.0	31.0	39.0	10.0	40.0
46	31.62716549620736	35.0	31.0	39.0	10.0	40.0
47	31.725844444639765	35.0	31.0	39.0	10.0	40.0
48	31.580846656646713	35.0	31.0	38.0	10.0	40.0
49	31.601050630055756	35.0	31.0	38.0	10.0	40.0
50	31.43737090522356	35.0	31.0	38.0	10.0	40.0
51	29.997108277007726	34.0	27.0	37.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	39.0
9	75.0
10	100.0
11	80.0
12	77.0
13	84.0
14	119.0
15	208.0
16	409.0
17	803.0
18	1501.0
19	2509.0
20	3919.0
21	5642.0
22	7928.0
23	11011.0
24	15273.0
25	19701.0
26	22014.0
27	22056.0
28	21245.0
29	22203.0
30	25425.0
31	32115.0
32	42479.0
33	58813.0
34	82449.0
35	66780.0
36	54962.0
37	64810.0
38	68606.0
39	29200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.258286236629075	3.9480661785602327	47.79150882312441	30.002138761686282
2	29.28594657783481	5.404914464181601	48.256323263574544	17.052815694409045
3	20.244844265921323	5.020669813283175	53.816224704748336	20.918261216047156
4	16.613051133983163	4.688282808750758	57.61238020737199	21.086285849894086
5	15.30210741271362	4.951233303742247	59.13295187200244	20.613707411541697
6	18.352333154614893	7.644901104245588	60.14769174877461	13.855073992364913
7	75.85528493872302	1.8676077218086304	19.745604551753637	2.5315027877147185
8	78.75550438153165	3.221180186277353	16.284033411559275	1.7392820206317259
9	75.44613689832678	3.4248020180534926	16.91980229638549	4.20925878723423
10	48.42595929321251	20.411989956609506	21.39713874703723	9.764912003140756
11	44.51129295468462	16.276415904183477	28.528444065522287	10.68384707560962
12	38.74234953225575	18.366396245154824	29.656567609772676	13.234686612816748
13	20.71962006217058	35.2973318215511	32.30204002707145	11.681008089206872
14	14.317837565444643	37.099165296980246	34.99644028020708	13.586556857368034
15	13.098743404264045	19.98907180672626	54.65605489293007	12.25612989607962
16	14.11333679050976	16.711639258289164	52.89040457753597	16.284619373665105
17	15.008540397692482	14.436494891875343	39.31629941491684	31.23866529551534
18	19.89839417084897	18.734233957089998	42.74403124349948	18.623340628561554
19	24.087437265432044	19.83467079183989	36.38136171733774	19.696530225390322
20	28.138046812512634	19.459508553581838	35.86937732736824	16.533067306537287
21	21.70989602102432	24.048763766447223	36.85833487148386	17.383005341044594
22	23.033291437042767	20.00709014148055	35.87186766631802	21.087750755158662
23	18.262094990316978	25.55937407527855	33.835356367503714	22.343174566900757
24	19.73725459174555	19.730662518054956	44.56710584526499	15.964977044934503
25	15.966295459672622	21.117781313082485	43.49655307791245	19.419370149332444
26	14.938517926045721	29.630199315010298	35.78719614202549	19.644086616918486
27	15.426184888623254	31.38076110617926	36.28379902671694	16.909254978480543
28	12.965290534661122	26.83940829546553	45.497613669324004	14.697687500549339
29	14.098541247337534	22.15639914566725	43.88372753934003	19.861332067655184
30	16.1974575104228	26.059346242078522	38.802703629156305	18.940492618342372
31	24.171815808671653	26.069307597877646	32.51327936622339	17.245597227227314
32	25.841661319762448	25.85806825872571	32.78912102754315	15.511149393968694
33	25.718609277538025	26.044843679959218	31.581013655846878	16.655533386655886
34	18.869298222483952	27.330005068572216	33.24119079219147	20.559505916752364
35	18.27938087243898	26.374007892909567	34.49075498287526	20.8558562517762
36	27.826168481684288	24.646884585973826	30.347856404126343	17.17909052821554
37	20.848092253873943	28.456810198084494	32.9048485434447	17.790249004596873
38	18.97931260785365	30.87888456253534	28.6809407035647	21.460862126046308
39	21.03911590037472	27.664442940475038	31.836493133989023	19.459948025161214
40	21.678840029415298	26.037665644162793	29.713405934038246	22.570088392383663
41	16.037343365004585	24.731409619739892	32.21224133435291	27.019005680902612
42	19.611858701097802	26.43421549928366	29.32520603892546	24.628719760693077
43	22.57213925975407	27.52322607296986	27.477374538188613	22.42726012908745
44	19.61434904004758	29.691725336122516	29.06811516499228	21.625810458837627
45	16.595179289755333	35.142637825611814	26.671969623724433	21.590213260908417
46	23.304445401515885	31.03958467005939	28.517896747617332	17.138073180807396
47	20.64227306420094	28.48142060652938	30.7268273960723	20.14947893319739
48	22.954626024335006	25.379776689841467	31.28114754818806	20.384449737635467
49	22.320908006879193	23.74186611351844	32.80171921281851	21.13550666678386
50	20.572690064133553	29.235114365154008	29.98836865219926	20.20382691851318
51	17.24925949038876	31.272944078706434	27.419803761290755	24.057992669614055
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18.0
1	331.5
2	645.0
3	6857.5
4	13070.0
5	11705.5
6	10341.0
7	10183.5
8	10026.0
9	10357.0
10	10688.0
11	10746.5
12	10805.0
13	10415.0
14	10025.0
15	9408.0
16	8791.0
17	7922.5
18	7054.0
19	6310.0
20	5566.0
21	5083.5
22	4601.0
23	4274.5
24	3948.0
25	4385.5
26	5538.5
27	6254.0
28	7326.5
29	8399.0
30	9687.0
31	10975.0
32	12045.0
33	13115.0
34	14080.5
35	15046.0
36	17007.0
37	18968.0
38	22886.5
39	26805.0
40	34310.0
41	41815.0
42	51589.0
43	61363.0
44	60299.5
45	59236.0
46	60111.0
47	60986.0
48	54516.0
49	48046.0
50	43955.5
51	39865.0
52	40308.0
53	40751.0
54	34610.0
55	28469.0
56	26938.0
57	25407.0
58	24359.0
59	23311.0
60	19896.5
61	16482.0
62	14615.0
63	12748.0
64	10809.5
65	8871.0
66	7415.0
67	5959.0
68	4751.5
69	3544.0
70	2713.5
71	1883.0
72	1914.0
73	1945.0
74	1457.0
75	655.5
76	342.0
77	351.5
78	361.0
79	244.5
80	128.0
81	84.0
82	40.0
83	48.5
84	57.0
85	39.5
86	22.0
87	22.5
88	23.0
89	25.0
90	27.0
91	22.0
92	17.0
93	11.5
94	6.0
95	4.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	682638.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.312703765938956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.57138166482714	23.661317723864734
2	7.493480601036517	4.243213928659142
3	2.461478250622183	2.0907331360850256
4	1.2313074148003498	1.3944656832018572
5	0.7614196576105946	1.0778924603745716
6	0.5310289259128445	0.9020918802309067
7	0.38064444004562636	0.7543951289814473
8	0.3145035765641059	0.7123557277270267
9	0.2466895054783198	0.628600220169628
>10	1.6125723137191836	9.644838451325997
>50	0.5664771441919559	11.980358589969185
>100	0.8174878306562908	39.450554668296725
>500	0.008384490570833753	1.5131975684734684
>1k	0.003144183964062658	1.94598483264026
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4527	0.6631626132737996	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGC	2520	0.36915612667328807	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCG	1831	0.2682241539439645	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTC	1753	0.25679789288026744	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1463	0.2143156402075478	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1022	0.14971331803972238	No Hit
TGTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	880	0.1289116632827355	No Hit
TTGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	845	0.12378449485671761	No Hit
TTTTCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	758	0.11103981905490172	No Hit
TTTCCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	728	0.1066451032611721	No Hit
TTTTTTGGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAG	694	0.10166442536161187	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03017704845027672	0.0
2	0.0	0.0	0.0	0.1451721117195351	0.0
3	0.0	0.0	0.0	0.17183338753482813	0.0
4	0.0	0.0	0.0	0.30733712450815803	0.0
5	0.0	0.0	0.0	0.7415350449286444	0.0
6	0.0	0.0	0.0	0.839097735549442	0.0
7	0.0	0.0	0.0	0.9466217819693601	0.0
8	0.0	0.0	0.0	1.0917938936888951	0.0
9	0.0	0.0	0.0	1.1448234642665658	0.0
10	0.0	0.0	0.0	1.5599776162475574	0.0
11	0.0	0.0	0.0	1.6614955510827114	0.0
12	0.0	0.0	0.0	2.2783671579958926	0.0
13	0.0	0.0	0.0	2.409329688649035	0.0
14	0.0	0.0	0.0	2.476568840293098	0.0
15	0.0	0.0	0.0	2.572813116175777	0.0
16	0.0	0.0	0.0	2.6541153583597747	0.0
17	0.0	0.0	0.0	2.7406912594962485	0.0
18	0.0	0.0	0.0	2.8766344680489513	0.0
19	0.0	0.0	0.0	2.9771269691989017	0.0
20	0.0	0.0	0.0	3.056671325065408	0.0
21	0.0	0.0	0.0	3.1313814935588113	0.0
22	0.0	0.0	0.0	3.193786457829772	0.0
23	0.0	0.0	0.0	3.252382668412834	0.0
24	0.0	0.0	0.0	3.3061446916227926	0.0
25	0.0	0.0	0.0	3.35126377377175	0.0
26	0.0	0.0	0.0	3.3985802138175725	0.0
27	0.0	0.0	0.0	3.446336125442768	0.0
28	0.0	0.0	0.0	3.4874999633773682	0.0
29	0.0	0.0	0.0	3.533790969737987	0.0
30	0.0	0.0	0.0	3.599858197170389	0.0
31	0.0	0.0	0.0	3.661091237229688	0.0
32	0.0	0.0	0.0	3.7161716751777663	0.0
33	0.0	0.0	0.0	3.7746213952343703	0.0
34	0.0	0.0	0.0	3.8383447742434496	0.0
35	0.0	0.0	0.0	3.9096856606283272	0.0
36	0.0	0.0	0.0	3.965645041735151	0.0
37	0.0	0.0	0.0	4.035228041802537	0.0
38	0.0	0.0	0.0	4.0954356481766325	0.0
39	0.0	0.0	0.0	4.152566953495118	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTCC	35	1.2109558E-7	45.000004	35
GTTGAAT	25	3.889134E-5	45.000004	25
TGTCACG	35	1.2109558E-7	45.000004	28
AACCGTC	25	3.889134E-5	45.000004	11
AACGTAG	25	3.889134E-5	45.000004	20
CTATGCG	25	3.889134E-5	45.000004	23
GATACGA	50	2.1827873E-11	45.000004	19
CTCACAT	25	3.889134E-5	45.000004	38
GTACATT	35	1.2109558E-7	45.000004	33
AGTCATC	45	3.8380676E-10	45.000004	12
ATTAGGT	25	3.889134E-5	45.000004	20
AGTCAAT	25	3.889134E-5	45.000004	20
CACGGGT	35	1.2109558E-7	45.000004	23
CAACGAG	25	3.889134E-5	45.000004	35
TAGACCG	25	3.889134E-5	45.000004	25
TCCGAAC	35	1.2109558E-7	45.000004	43
CGCATAC	45	3.8380676E-10	45.000004	37
TAGAACG	25	3.889134E-5	45.000004	17
TAGCGGA	25	3.889134E-5	45.000004	29
TTCACTC	25	3.889134E-5	45.000004	12
>>END_MODULE