FastQCFastQC Report
Sat 18 Jun 2016
SRR3548173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863822
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC139851.6189677966062452No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG104181.2060355026845808No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC81310.9412818844623081No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53320.6172567959602788No Hit
GAACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT20570.2381277624325382No Hit
GATTGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC19630.22724589093586411No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTC19570.2265513033935232No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTA17450.20200921023081145No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCT17260.19980968301339858No Hit
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC17160.1986520371094971TruSeq Adapter, Index 16 (95% over 21bp)
GATTGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG16190.18742287184165257No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTGTCTTT14890.1723734750909331No Hit
GATTCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC13580.15720831374982344No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA13300.15396690521889927No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13190.15269349472460764No Hit
TTTTTTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA8900.10303048544723335No Hit
TTTTTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC8840.10233589790489245No Hit
CTTTTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC8730.1010624874106008No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAGA302.164872E-645.00000425
CGAACTT302.164872E-645.00000422
GATCGCT302.164872E-645.0000049
GCGAACT302.164872E-645.00000421
CGCATAC302.164872E-645.00000420
ATGTCGC302.164872E-645.00000427
TCTAGCG302.164872E-645.0000041
CGTAGTC302.164872E-645.00000430
AGGTTAC302.164872E-645.00000434
GTACTAC302.164872E-645.00000442
CGTCCGT302.164872E-645.00000444
CTTAGGT302.164872E-645.00000412
GTAGTCA302.164872E-645.00000431
ATCGAGC302.164872E-645.00000441
TGATCCG207.032312E-445.04
TCACGAC207.032312E-445.025
GTGCACG253.8899983E-545.01
AGCCGTC253.8899983E-545.015
AATCCCG253.8899983E-545.031
ACCGGTG253.8899983E-545.017
Produced by FastQC (version 0.11.5)