SRR3548169_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548169_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	662292
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC	9754	1.4727642792001112	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG	7362	1.1115942816763602	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC	5747	0.8677441370271722	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3954	0.5970176296859996	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT	1610	0.24309519064098614	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTC	1398	0.21108514069322895	No Hit
GATTGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC	1348	0.20353560061121076	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT	1259	0.19009741926521836	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTA	1200	0.1811889619684369	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	1192	0.17998103555531397	Illumina Single End Adapter 1 (95% over 21bp)
TTGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1185	0.1789240999438314	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1166	0.17605527471266452	No Hit
GATTGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG	1065	0.16080520374698773	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCAGGT	1017	0.15355764526825028	No Hit
GATTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC	976	0.14736702240099533	No Hit
TTTTGCGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC	959	0.14480017877310916	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTT	767	0.11580994485815924	No Hit
TCTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	693	0.1046366255367723	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACTCG	20	7.031118E-4	45.000004	22
TACCCGT	20	7.031118E-4	45.000004	35
CATATAG	20	7.031118E-4	45.000004	43
CGAGTTA	20	7.031118E-4	45.000004	14
GGTAAGC	20	7.031118E-4	45.000004	12
GGTAACG	20	7.031118E-4	45.000004	17
CATGCTT	20	7.031118E-4	45.000004	42
CACGGCA	40	6.8066583E-9	45.000004	38
CGAAACG	20	7.031118E-4	45.000004	22
ACGTTGT	20	7.031118E-4	45.000004	36
GCGAATG	20	7.031118E-4	45.000004	4
CCGATAG	20	7.031118E-4	45.000004	12
CCTAGAA	20	7.031118E-4	45.000004	36
GACAATC	20	7.031118E-4	45.000004	9
TCTTATC	20	7.031118E-4	45.000004	13
ATTACGG	20	7.031118E-4	45.000004	27
TTCACGC	20	7.031118E-4	45.000004	26
GCTGCGA	20	7.031118E-4	45.000004	29
TTCGTAG	40	6.8066583E-9	45.000004	1
TTGTACG	40	6.8066583E-9	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content