##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548169_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 662292 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.74924806580783 30.0 25.0 31.0 16.0 33.0 2 28.434091005175965 31.0 28.0 33.0 16.0 34.0 3 26.363096640152683 30.0 22.0 31.0 10.0 34.0 4 30.458124211073063 33.0 28.0 35.0 19.0 37.0 5 32.52702886340164 35.0 32.0 35.0 28.0 37.0 6 33.71168608408376 35.0 33.0 37.0 30.0 37.0 7 33.53738683239417 35.0 32.0 35.0 29.0 37.0 8 33.599075634312356 35.0 33.0 37.0 28.0 37.0 9 35.41133669136876 37.0 35.0 39.0 30.0 39.0 10 35.6910803693839 37.0 35.0 39.0 30.0 39.0 11 35.69291339771581 37.0 35.0 39.0 30.0 39.0 12 34.566547685914976 35.0 33.0 39.0 27.0 39.0 13 34.31057750961811 37.0 33.0 39.0 26.0 39.0 14 35.16687503397293 37.0 33.0 40.0 26.0 41.0 15 35.987398307695095 38.0 34.0 40.0 30.0 41.0 16 35.75017363942189 37.0 34.0 40.0 30.0 41.0 17 35.60354647194893 37.0 34.0 40.0 30.0 41.0 18 35.42711523014018 37.0 34.0 39.0 29.0 40.0 19 34.73424712966486 36.0 33.0 39.0 27.0 40.0 20 34.8185377446806 35.0 34.0 39.0 29.0 40.0 21 35.04208566614122 35.0 34.0 39.0 29.0 40.0 22 35.25799647285487 35.0 34.0 39.0 30.0 41.0 23 35.46198655577902 35.0 34.0 39.0 30.0 41.0 24 35.345293314731265 35.0 34.0 40.0 30.0 41.0 25 34.58662342290108 35.0 33.0 39.0 27.0 40.0 26 34.5810201542522 35.0 33.0 39.0 27.0 40.0 27 34.843821154415274 35.0 34.0 39.0 29.0 41.0 28 34.75210481177486 35.0 34.0 39.0 27.0 41.0 29 34.78029177462509 36.0 34.0 40.0 27.0 41.0 30 34.39211858213598 35.0 33.0 39.0 25.0 41.0 31 34.20742210384543 35.0 33.0 39.0 24.0 41.0 32 33.75261063096036 35.0 33.0 39.0 23.0 41.0 33 33.48131186848097 35.0 33.0 40.0 20.0 41.0 34 33.23313281754876 35.0 33.0 40.0 18.0 41.0 35 33.01510813961213 35.0 33.0 40.0 15.0 41.0 36 32.715331002035356 35.0 32.0 40.0 15.0 41.0 37 32.60971444619594 35.0 32.0 40.0 13.0 41.0 38 32.53455877467945 35.0 32.0 40.0 12.0 41.0 39 32.386838433802616 35.0 31.0 40.0 12.0 41.0 40 32.28691272127702 35.0 31.0 40.0 10.0 41.0 41 32.095056259172694 35.0 31.0 39.0 10.0 41.0 42 32.13367517650825 35.0 31.0 39.0 10.0 41.0 43 32.1454071617957 35.0 31.0 39.0 10.0 41.0 44 31.984490224855502 35.0 31.0 39.0 10.0 40.0 45 31.970922191420097 35.0 31.0 39.0 10.0 40.0 46 31.849987618754266 35.0 31.0 39.0 10.0 40.0 47 31.88261522108073 35.0 31.0 39.0 10.0 40.0 48 31.761325820031043 35.0 31.0 39.0 10.0 40.0 49 31.77337186618591 35.0 31.0 39.0 10.0 40.0 50 31.619593774347265 35.0 31.0 39.0 10.0 40.0 51 29.92069963097848 34.0 27.0 37.0 8.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 33.0 9 85.0 10 91.0 11 100.0 12 105.0 13 82.0 14 92.0 15 164.0 16 403.0 17 734.0 18 1400.0 19 2433.0 20 3719.0 21 5400.0 22 7417.0 23 10228.0 24 14554.0 25 18438.0 26 21026.0 27 20676.0 28 19967.0 29 20574.0 30 24209.0 31 30847.0 32 40485.0 33 55196.0 34 76346.0 35 65392.0 36 57664.0 37 66451.0 38 68899.0 39 29079.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.551080188194934 4.576078225314514 43.37663749524379 29.496204091246764 2 25.824560767758026 10.558182795504099 47.610721554842875 16.006534881894996 3 19.916592681173864 9.325795872515446 52.149354061350586 18.60825738496011 4 15.91382652968781 4.370126771877057 59.020341480796986 20.695705217638142 5 20.03647937767631 4.753794398845222 54.8019604645685 20.407765758909967 6 18.27335978692178 11.12666316368007 57.06652050757069 13.533456541827471 7 72.54398362051784 2.264862024605461 22.69754126578609 2.493613089090613 8 73.36763844346602 7.296630489270593 17.208874635357212 2.1268564319061682 9 70.04870963260919 3.412392117072228 18.00399219679537 8.534906053523219 10 46.153509328211726 21.16679651875608 22.80127194651302 9.87842220651918 11 41.268503922741026 15.780199670236088 30.95734207872056 11.99395432830232 12 35.2016935128312 17.325288543421934 32.58804273643649 14.884975207310372 13 19.403827918803188 32.753075682629415 35.508959794169336 12.334136604398061 14 15.112971317787322 34.96614786227223 34.91737783334239 15.003502986598056 15 12.534199416571543 20.236693180651436 54.85314634632458 12.375961056452441 16 14.311210161076987 16.475361320988323 49.75146914049996 19.461959377434727 17 14.505988295193056 16.935128311983235 38.849480289660754 29.709403103162956 18 17.380853158425587 19.56312321453377 42.75108260404777 20.304941022992878 19 21.7650220748552 21.377730668647665 35.883265991435806 20.97398126506133 20 25.561987763705435 19.557385564071435 37.39241905383124 17.488207618391886 21 21.107457133711414 25.71328054694908 36.439365113877265 16.739897205462242 22 21.4085327921823 19.07330905401243 35.685921013691846 23.832237140113424 23 17.77614707712006 27.32797617969113 34.36520447174358 20.530672271445223 24 18.34689230732064 19.44263255482476 43.444583355981955 18.76589178187265 25 14.413128952184234 26.117331932138693 41.560973105518414 17.908566010158662 26 15.736865310165305 26.950046203185302 36.34877063289304 20.96431785375635 27 17.25205800462636 29.641759224028075 36.42517197852307 16.6810107928225 28 13.496463795425583 25.24822887789676 45.65508869199688 15.600218634680777 29 15.304427654267302 20.712767178223505 43.33058530074348 20.652219866765716 30 16.0589286900642 26.03670284406274 40.41163112343196 17.492737342441096 31 25.361169997523753 22.502008177661818 33.23730318349006 18.89951864132437 32 25.282201808265842 25.0729285571923 33.889734437378074 15.755135197163789 33 22.570407010804903 25.097389067058035 31.336178000036234 20.996025922100824 34 21.212093759248187 26.519873409311906 32.77074160642134 19.49729122501857 35 20.252698205625315 24.198691815694588 34.56602223792527 20.98258774075483 36 28.547679875342 22.422436025197346 30.083105337222854 18.946778762237805 37 18.797448859415482 26.98522705996751 37.16200709052804 17.055316990088965 38 22.291376009373508 27.986749047248043 27.26773084983663 22.45414409354182 39 21.90544352038074 28.669831433869046 31.630006100028385 17.794718945721826 40 22.550929197393295 24.168040682961596 29.691586188569392 23.589443931075717 41 17.469786740591765 27.415701835444185 30.187137999553066 24.927373424410984 42 22.713697281561608 23.94668816775682 31.647823014621952 21.691791536059625 43 24.44344790515362 25.11324310123027 28.718450471997247 21.724858521618863 44 20.163311651054215 28.13577696846708 31.667753800438476 20.03315758004022 45 17.914907623827556 32.0018964444686 26.710574791783685 23.372621139920156 46 24.242781129773576 30.273957710496273 27.21292118884118 18.270339970888973 47 19.988917275159597 25.65680998713558 34.108973081359885 20.245299656344937 48 22.87284158649055 26.428976946724404 30.13383824657402 20.564343220211025 49 20.300562289745308 23.783165129580304 34.38015256110597 21.536120019568408 50 20.736925706485962 28.89526070071811 29.455436574803862 20.912377017992064 51 18.954177311518183 28.689762219685576 27.31559493395662 25.04046553483962 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9.0 1 209.0 2 409.0 3 5782.5 4 11156.0 5 10269.0 6 9382.0 7 9451.5 8 9521.0 9 9809.0 10 10097.0 11 10009.0 12 9921.0 13 9369.5 14 8818.0 15 8161.0 16 7504.0 17 6888.0 18 6272.0 19 5748.5 20 5225.0 21 4881.0 22 4537.0 23 4725.0 24 4913.0 25 5053.0 26 5993.0 27 6793.0 28 6597.0 29 6401.0 30 8443.5 31 10486.0 32 11388.0 33 12290.0 34 13994.0 35 15698.0 36 17441.5 37 19185.0 38 22758.5 39 26332.0 40 32782.5 41 39233.0 42 44334.0 43 49435.0 44 54292.0 45 59149.0 46 73290.5 47 87432.0 48 68362.5 49 49293.0 50 45076.5 51 40860.0 52 36296.5 53 31733.0 54 28386.0 55 25039.0 56 23614.0 57 22189.0 58 21168.5 59 20148.0 60 18136.0 61 16124.0 62 14062.5 63 12001.0 64 9313.5 65 6626.0 66 5744.0 67 4862.0 68 3896.0 69 2930.0 70 2652.5 71 2375.0 72 1839.0 73 1303.0 74 942.5 75 455.0 76 328.0 77 310.0 78 292.0 79 209.0 80 126.0 81 84.5 82 43.0 83 33.0 84 23.0 85 15.5 86 8.0 87 6.5 88 5.0 89 4.5 90 4.0 91 3.0 92 2.0 93 2.0 94 2.0 95 2.5 96 3.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 662292.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.117187495542556 #Duplication Level Percentage of deduplicated Percentage of total 1 83.65015814416937 23.520071805713965 2 7.344182018147458 4.129954856112883 3 2.392639261344855 2.018228601612892 4 1.2330450297876046 1.3867903317193975 5 0.7943875839658937 1.1167972321250046 6 0.5261157203337457 0.8875736611785814 7 0.38593360470102506 0.7595957266946529 8 0.30724269053704 0.6911040269171931 9 0.2124631940633145 0.5376480717042051 >10 1.6630260430777675 10.239123006587091 >50 0.7456743121714927 15.608591550238007 >100 0.7332102793372307 32.28103817577777 >500 0.00433531576843891 0.8418503711031551 >1k 0.0059610591816035025 2.497971942781018 >5k 0.0016257434131645915 3.483660639734196 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC 9754 1.4727642792001112 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG 7362 1.1115942816763602 No Hit GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC 5747 0.8677441370271722 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3954 0.5970176296859996 No Hit GAACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT 1610 0.24309519064098614 No Hit GAATGATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTC 1398 0.21108514069322895 No Hit GATTGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC 1348 0.20353560061121076 No Hit GAATGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT 1259 0.19009741926521836 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTA 1200 0.1811889619684369 No Hit GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC 1192 0.17998103555531397 Illumina Single End Adapter 1 (95% over 21bp) TTGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA 1185 0.1789240999438314 No Hit TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA 1166 0.17605527471266452 No Hit GATTGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG 1065 0.16080520374698773 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCAGGT 1017 0.15355764526825028 No Hit GATTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC 976 0.14736702240099533 No Hit TTTTGCGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC 959 0.14480017877310916 No Hit GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTT 767 0.11580994485815924 No Hit TCTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA 693 0.1046366255367723 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.05601758740857507 0.0 2 0.0 0.0 0.0 0.34350407373182823 0.0 3 0.0 0.0 0.0 0.3895562682321393 0.0 4 0.0 0.0 0.0 0.7612956218707156 0.0 5 0.0 0.0 0.0 2.2372307079052742 0.0 6 0.0 0.0 0.0 2.320879612014036 0.0 7 0.0 0.0 0.0 2.6212003164767204 0.0 8 0.0 0.0 0.0 2.9696870866626806 0.0 9 0.0 0.0 0.0 3.0432196070615376 0.0 10 0.0 0.0 0.0 4.611259082096718 0.0 11 0.0 0.0 0.0 4.746093867961564 0.0 12 0.0 0.0 0.0 6.829917921400228 0.0 13 0.0 0.0 0.0 7.063047719132951 0.0 14 0.0 0.0 0.0 7.176139829561583 0.0 15 0.0 0.0 0.0 7.558750520918266 0.0 16 0.0 0.0 0.0 7.723934457912824 0.0 17 0.0 0.0 0.0 7.893195146551672 0.0 18 0.0 0.0 0.0 8.013383824657401 0.0 19 0.0 0.0 0.0 8.328652618482481 0.0 20 0.0 0.0 0.0 8.445670489753764 0.0 21 0.0 0.0 0.0 8.517995083739498 0.0 22 0.0 0.0 0.0 8.606777675104032 0.0 23 0.0 0.0 0.0 8.660379409686362 0.0 24 0.0 0.0 0.0 8.768790805264143 0.0 25 0.0 0.0 0.0 8.812578137739848 0.0 26 0.0 0.0 0.0 8.861046185066407 0.0 27 0.0 0.0 0.0 8.926425202176683 0.0 28 0.0 0.0 0.0 8.96190804056217 0.0 29 0.0 0.0 0.0 9.004789428228033 0.0 30 0.0 0.0 0.0 9.064732776479257 0.0 31 0.0 0.0 0.0 9.117428566251744 0.0 32 0.0 0.0 0.0 9.176012997288206 0.0 33 0.0 0.0 0.0 9.221914200986875 0.0 34 0.0 0.0 0.0 9.284877365270908 0.0 35 0.0 0.0 0.0 9.346028639935255 0.0 36 0.0 0.0 0.0 9.396610558484777 0.0 37 0.0 0.0 0.0 9.453534090703194 0.0 38 0.0 0.0 0.0 9.499133312798584 0.0 39 0.0 0.0 0.0 9.552584056579272 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACTCG 20 7.031118E-4 45.000004 22 TACCCGT 20 7.031118E-4 45.000004 35 CATATAG 20 7.031118E-4 45.000004 43 CGAGTTA 20 7.031118E-4 45.000004 14 GGTAAGC 20 7.031118E-4 45.000004 12 GGTAACG 20 7.031118E-4 45.000004 17 CATGCTT 20 7.031118E-4 45.000004 42 CACGGCA 40 6.8066583E-9 45.000004 38 CGAAACG 20 7.031118E-4 45.000004 22 ACGTTGT 20 7.031118E-4 45.000004 36 GCGAATG 20 7.031118E-4 45.000004 4 CCGATAG 20 7.031118E-4 45.000004 12 CCTAGAA 20 7.031118E-4 45.000004 36 GACAATC 20 7.031118E-4 45.000004 9 TCTTATC 20 7.031118E-4 45.000004 13 ATTACGG 20 7.031118E-4 45.000004 27 TTCACGC 20 7.031118E-4 45.000004 26 GCTGCGA 20 7.031118E-4 45.000004 29 TTCGTAG 40 6.8066583E-9 45.000004 1 TTGTACG 40 6.8066583E-9 45.000004 1 >>END_MODULE