##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548167_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	247775
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.679164564625165	30.0	25.0	31.0	16.0	33.0
2	28.534949046513976	31.0	28.0	33.0	16.0	34.0
3	26.643551609322973	31.0	23.0	33.0	10.0	34.0
4	30.48082332761578	33.0	28.0	35.0	19.0	37.0
5	32.459210977701545	35.0	32.0	35.0	28.0	37.0
6	33.655453536474624	35.0	33.0	37.0	28.0	37.0
7	33.28090404600948	35.0	32.0	35.0	28.0	37.0
8	33.51452325698719	35.0	33.0	37.0	28.0	37.0
9	35.198123297346385	37.0	35.0	39.0	29.0	39.0
10	35.568870951468064	37.0	35.0	39.0	30.0	39.0
11	35.64860861668853	37.0	35.0	39.0	30.0	39.0
12	34.70295631116941	37.0	33.0	39.0	27.0	39.0
13	34.62828372515387	37.0	33.0	39.0	27.0	39.0
14	35.475055998385635	38.0	33.0	40.0	27.0	41.0
15	36.18847341337907	38.0	34.0	40.0	30.0	41.0
16	35.895546362627385	38.0	34.0	40.0	30.0	41.0
17	35.81462213701948	37.0	34.0	40.0	30.0	41.0
18	35.54691958430027	37.0	34.0	39.0	29.0	41.0
19	35.00067803450711	37.0	34.0	39.0	27.0	40.0
20	35.29465846029664	36.0	34.0	39.0	29.0	40.0
21	35.401969528806376	37.0	34.0	39.0	30.0	40.0
22	35.64399959640803	37.0	34.0	40.0	30.0	41.0
23	35.83283220663909	37.0	34.0	40.0	30.0	41.0
24	35.717800423771564	37.0	34.0	40.0	30.0	41.0
25	34.99273938048633	36.0	34.0	40.0	27.0	41.0
26	34.990378367470484	36.0	34.0	40.0	27.0	41.0
27	35.30905054989406	36.0	34.0	40.0	29.0	41.0
28	35.268186863081425	37.0	34.0	40.0	29.0	41.0
29	35.288209060639694	37.0	34.0	40.0	29.0	41.0
30	34.907371607305016	36.0	34.0	40.0	27.0	41.0
31	34.78249218040561	36.0	34.0	40.0	26.0	41.0
32	34.446933710019174	36.0	33.0	40.0	24.0	41.0
33	34.35650085763293	37.0	33.0	40.0	23.0	41.0
34	34.14791645646252	37.0	34.0	40.0	22.0	41.0
35	33.9828231258198	37.0	33.0	40.0	20.0	41.0
36	33.71656139642821	37.0	33.0	40.0	18.0	41.0
37	33.609996973060234	37.0	33.0	40.0	18.0	41.0
38	33.53079204923822	36.0	33.0	40.0	18.0	41.0
39	33.39342548683281	36.0	33.0	40.0	18.0	41.0
40	33.32008475431339	36.0	33.0	40.0	18.0	41.0
41	33.167405912622336	36.0	32.0	40.0	17.0	41.0
42	33.16263141963475	36.0	33.0	40.0	17.0	41.0
43	33.15020078700434	36.0	33.0	40.0	17.0	41.0
44	32.99092725254767	36.0	32.0	40.0	15.0	41.0
45	32.975566542225806	35.0	32.0	40.0	17.0	41.0
46	32.82915144788619	35.0	32.0	39.0	16.0	41.0
47	32.91765916658259	35.0	32.0	40.0	17.0	41.0
48	32.80711129048532	35.0	32.0	39.0	16.0	41.0
49	32.7745050953486	35.0	32.0	39.0	15.0	40.0
50	32.64899202905862	35.0	32.0	39.0	15.0	40.0
51	31.087284835031785	34.0	29.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	27.0
9	37.0
10	46.0
11	30.0
12	34.0
13	33.0
14	49.0
15	63.0
16	128.0
17	251.0
18	436.0
19	770.0
20	1224.0
21	1749.0
22	2343.0
23	3222.0
24	4331.0
25	5655.0
26	6490.0
27	6652.0
28	6696.0
29	7227.0
30	8471.0
31	10723.0
32	14186.0
33	19179.0
34	25824.0
35	24155.0
36	24130.0
37	29273.0
38	31141.0
39	13200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.544445565533245	4.1331853496115425	48.355968116234486	26.966400968620725
2	25.069518716577537	5.824235697709615	51.80910099889012	17.29714458682272
3	20.46574513167188	5.46544243769549	53.83916860054485	20.22964383008778
4	16.673594995459588	4.727676319241247	56.32690949450105	22.271819190798105
5	17.245888406820704	5.6091211784885475	54.38240339017254	22.762587024518215
6	19.87892240944405	8.311572999697306	57.253960246191106	14.55554434466754
7	78.60478256482696	1.649076783372011	16.90243164161033	2.843709010190697
8	81.11229946524064	3.443850267379679	13.270911108868933	2.172939158510746
9	77.14095449500556	4.406013520330945	13.864594894561598	4.588437090101907
10	42.37433155080214	29.220058520835433	18.969629704368884	9.435980223993543
11	35.629502572898794	21.339521743517302	29.857733831096763	13.173241852487136
12	33.37342346887297	19.817172838260518	31.749369387549187	15.060034305317323
13	22.46352537584502	29.136111391383313	33.038038543033	15.362324689738674
14	15.698920391484208	32.32408435072142	34.11805065079205	17.858944607002318
15	13.825850065583694	22.992230854605992	47.736050852588036	15.44586822722228
16	16.58682272222783	20.06578549086873	45.89244274038947	17.454949046513974
17	17.634951064473817	19.145999394612048	36.07305014630209	27.145999394612048
18	19.4890525678539	21.385531227928563	39.07093128846736	20.054484915750177
19	23.678740793058218	22.79366360609424	34.095449500554935	19.432146100292606
20	25.968721622439713	21.985268893149026	33.991726364645345	18.054283119765916
21	22.079305821814145	24.104126727878114	36.20906063969327	17.607506810614467
22	22.46231459993946	21.69306830794067	34.386035717889214	21.458581374230654
23	19.997578448188882	26.509534860256277	32.53597013419433	20.956916557360508
24	19.698920391484208	21.913025930783977	39.5786499848653	18.80940369286651
25	17.598224195338513	23.60528705478761	38.55756230451014	20.238926445363738
26	17.495711835334475	28.947230350116033	33.58450206840884	19.972555746140653
27	17.8585410150338	29.37584502068409	34.22177378670164	18.543840177580467
28	15.782060336999294	27.15487841791948	39.61214811825245	17.450913126828777
29	16.92261124003632	24.123095550398546	39.17384724043991	19.780445969125214
30	18.832812027040664	25.687821612349914	35.27232368075875	20.20704267985067
31	22.72505297144587	26.388457269700332	31.088285743113715	19.798204015740087
32	24.58480476238523	27.123398244374936	31.32357986076077	16.96821713247906
33	24.16305115528201	25.64181212793865	29.95298153566744	20.242155181111894
34	20.46534153970336	27.149631722328728	32.753909797195035	19.631116940772877
35	21.51024114620119	25.24911714256886	31.18635859146403	22.054283119765916
36	24.351125012612247	25.05539299767935	29.129250327918477	21.46423166178993
37	21.913429522752498	29.04167087074967	30.807789324992434	18.237110281505398
38	21.286651195641205	28.488749873877513	27.94995459590354	22.274644334577744
39	20.920593280193724	28.86812632428615	28.89072747452326	21.32055292099687
40	21.706386842901825	25.611542730299668	29.890424780546866	22.79164564625164
41	18.596710725456564	26.13500151346988	29.62526485722934	25.643022903844216
42	20.575522147109275	28.730501463020886	28.988396730905052	21.705579658964787
43	22.945010594289172	27.014832004843104	28.135203309454145	21.904954091413583
44	20.453637372616285	28.999293714055092	29.104227625870244	21.442841287458382
45	19.704167087074968	30.59832509333064	26.375542326707695	23.321965492886694
46	21.15871254162042	31.18474422358995	28.430632630410656	19.225910604378974
47	20.44395116537181	27.245283018867923	31.58187872061346	20.728887095146806
48	22.422358995055998	25.267278781152253	29.63414388053678	22.67621834325497
49	20.7002320653819	26.082130965593787	31.875290081727375	21.34234688729694
50	20.984360811219858	29.040863686812635	28.927050751690043	21.047724750277467
51	20.102915951972555	29.174856220361217	27.339319947533042	23.382907880133185
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	74.5
2	142.0
3	1816.5
4	3491.0
5	3226.0
6	2961.0
7	2959.5
8	2958.0
9	2949.5
10	2941.0
11	2885.0
12	2829.0
13	2637.5
14	2446.0
15	2334.5
16	2223.0
17	2042.5
18	1862.0
19	1794.0
20	1726.0
21	1698.5
22	1671.0
23	1761.0
24	1851.0
25	2075.5
26	2517.5
27	2735.0
28	3099.0
29	3463.0
30	4003.5
31	4544.0
32	5368.5
33	6193.0
34	6700.5
35	7208.0
36	7572.0
37	7936.0
38	9058.5
39	10181.0
40	11946.5
41	13712.0
42	16211.0
43	18710.0
44	19105.0
45	19500.0
46	21559.0
47	23618.0
48	20627.0
49	17636.0
50	16883.5
51	16131.0
52	14805.5
53	13480.0
54	12332.5
55	11185.0
56	10273.5
57	9362.0
58	9150.0
59	8938.0
60	8393.5
61	7849.0
62	7248.5
63	6648.0
64	5172.0
65	3696.0
66	2954.5
67	2213.0
68	1836.0
69	1459.0
70	1118.5
71	778.0
72	654.5
73	531.0
74	422.5
75	233.5
76	153.0
77	104.5
78	56.0
79	71.5
80	87.0
81	57.5
82	28.0
83	19.0
84	10.0
85	9.5
86	9.0
87	5.0
88	1.0
89	1.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	247775.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.018767026536175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.62178194329101	27.76672384219554
2	7.0174397911970585	4.774492987589547
3	2.515126349507652	2.5668449197860963
4	1.2824771621781945	1.7451316718797298
5	0.7177601138925139	1.2208657047724751
6	0.4638747182346661	0.9468267581475129
7	0.35472772570886224	0.8447179901119968
8	0.29778146873887773	0.8104126727878115
9	0.24913987424368253	0.762788820502472
>10	4.871277731640764	42.77267682373121
>50	0.5504804840431843	11.525375845020685
>100	0.05338711590936054	2.6382806982141056
>500	0.002372760707082691	0.6538189890021189
>1k	0.002372760707082691	0.9710422762587024
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGC	1282	0.5174049036424175	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1124	0.45363737261628495	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCG	957	0.38623751387347394	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTC	663	0.26758147512864494	No Hit
TTTTTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC	261	0.10533750378367471	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	257	0.1037231359095954	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08515790535768339	0.0
2	0.0	0.0	0.0	0.3612148118252447	0.0
3	0.0	0.0	0.0	0.4411260215921703	0.0
4	0.0	0.0	0.0	0.6635051962465947	0.0
5	0.0	0.0	0.0	1.205125618000202	0.0
6	0.0	0.0	0.0	1.2781757643022904	0.0
7	0.0	0.0	0.0	1.4480879830491373	0.0
8	0.0	0.0	0.0	1.6809605488850772	0.0
9	0.0	0.0	0.0	1.7600645747149632	0.0
10	0.0	0.0	0.0	2.376753102613258	0.0
11	0.0	0.0	0.0	2.5636161840379375	0.0
12	0.0	0.0	0.0	3.437796387851882	0.0
13	0.0	0.0	0.0	3.5806679447079004	0.0
14	0.0	0.0	0.0	3.6521037231359097	0.0
15	0.0	0.0	0.0	3.7873070325900513	0.0
16	0.0	0.0	0.0	3.944304308344264	0.0
17	0.0	0.0	0.0	4.16224397134497	0.0
18	0.0	0.0	0.0	4.477045706790435	0.0
19	0.0	0.0	0.0	4.671173443648471	0.0
20	0.0	0.0	0.0	4.828170719402684	0.0
21	0.0	0.0	0.0	4.933911815154878	0.0
22	0.0	0.0	0.0	5.014226616890324	0.0
23	0.0	0.0	0.0	5.079204923822016	0.0
24	0.0	0.0	0.0	5.1340934315407125	0.0
25	0.0	0.0	0.0	5.169205932801937	0.0
26	0.0	0.0	0.0	5.216829785087277	0.0
27	0.0	0.0	0.0	5.275754212491171	0.0
28	0.0	0.0	0.0	5.322570880839471	0.0
29	0.0	0.0	0.0	5.3584905660377355	0.0
30	0.0	0.0	0.0	5.421854505095348	0.0
31	0.0	0.0	0.0	5.486832812027041	0.0
32	0.0	0.0	0.0	5.539299767934618	0.0
33	0.0	0.0	0.0	5.5877308041569975	0.0
34	0.0	0.0	0.0	5.638179800221976	0.0
35	0.0	0.0	0.0	5.6922611240036325	0.0
36	0.0	0.0	0.0	5.753607103218646	0.0
37	0.0	0.0	0.0	5.808495610937342	0.0
38	0.0	0.0	0.0	5.856523055191202	0.0
39	0.0	0.0	0.0	5.909797195035819	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCC	20	7.022563E-4	45.000004	6
TCCATTG	20	7.022563E-4	45.000004	10
TCGTTCC	20	7.022563E-4	45.000004	20
ATGATCC	20	7.022563E-4	45.000004	43
CTCGTCG	20	7.022563E-4	45.000004	1
CTCGTCA	20	7.022563E-4	45.000004	20
CGTATAG	20	7.022563E-4	45.000004	36
CGAAAGA	20	7.022563E-4	45.000004	44
GGCACCG	75	0.0	45.000004	8
TATGGGT	20	7.022563E-4	45.000004	4
ATAGCTA	20	7.022563E-4	45.000004	19
GCACGAT	20	7.022563E-4	45.000004	45
ATACTCG	20	7.022563E-4	45.000004	44
CGTTAGC	20	7.022563E-4	45.000004	26
GTTCGCG	20	7.022563E-4	45.000004	1
TCGTCAA	20	7.022563E-4	45.000004	21
TACCTAC	20	7.022563E-4	45.000004	11
GCGATGC	20	7.022563E-4	45.000004	9
CCCCATG	20	7.022563E-4	45.000004	38
GTGAGTC	20	7.022563E-4	45.000004	45
>>END_MODULE