FastQCFastQC Report
Sat 18 Jun 2016
SRR3548165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963290
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61830.6418627827549336No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGC42870.44503732001785545No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCG31910.3312605757352407No Hit
GAATCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTC24330.25257191499963666No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA14310.14855339513542132No Hit
GTTTTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC10640.11045479554443625No Hit
TTGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA10130.10516043974296421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT502.1827873E-1145.029
TCTGCGA253.8903334E-545.026
TATCATC253.8903334E-545.011
TGTCACG207.032718E-445.032
AGCGTCC253.8903334E-545.037
AGCCGAG253.8903334E-545.013
GTACCGT207.032718E-445.038
GTACCCG207.032718E-445.024
CGAACTA253.8903334E-545.028
ACACGGT207.032718E-445.040
CGTATAA207.032718E-445.028
CACGGTG207.032718E-445.04
CACGGAC207.032718E-445.030
ACGTTGA207.032718E-445.025
CGACGAT253.8903334E-545.020
TCCGATC351.211647E-745.018
CGTGATT253.8903334E-545.036
CATGACG207.032718E-445.014
CGGTTGA207.032718E-445.034
CTATACG207.032718E-445.010