##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548165_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	963290
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.776933218449273	30.0	25.0	31.0	16.0	33.0
2	28.577243613034497	31.0	28.0	33.0	16.0	34.0
3	26.53688920262849	31.0	23.0	33.0	10.0	34.0
4	30.4048147494524	33.0	28.0	35.0	19.0	37.0
5	32.5139386892836	35.0	32.0	35.0	28.0	37.0
6	33.73560609992837	35.0	33.0	37.0	30.0	37.0
7	33.493329111690144	35.0	32.0	35.0	29.0	37.0
8	33.606523476834596	35.0	33.0	37.0	29.0	37.0
9	35.34110496319904	37.0	35.0	39.0	30.0	39.0
10	35.719628564606715	37.0	35.0	39.0	30.0	39.0
11	35.72828327917865	37.0	35.0	39.0	30.0	39.0
12	34.56638395498759	37.0	33.0	39.0	27.0	39.0
13	34.405581911989124	37.0	33.0	39.0	26.0	39.0
14	35.266664244412375	37.0	33.0	40.0	27.0	41.0
15	36.10246758504708	38.0	34.0	40.0	30.0	41.0
16	35.880290462892795	37.0	34.0	40.0	30.0	41.0
17	35.79882278441591	37.0	34.0	40.0	30.0	41.0
18	35.49985777906965	37.0	34.0	39.0	29.0	40.0
19	34.787215687902915	37.0	33.0	39.0	27.0	40.0
20	35.02949786668604	35.0	34.0	39.0	29.0	40.0
21	35.13256651683294	35.0	34.0	39.0	29.0	40.0
22	35.345244941813995	36.0	34.0	39.0	30.0	41.0
23	35.53074982611675	36.0	34.0	40.0	30.0	41.0
24	35.373569745351865	35.0	34.0	40.0	30.0	41.0
25	34.637848415326644	35.0	33.0	39.0	27.0	41.0
26	34.62275015831162	35.0	33.0	39.0	27.0	41.0
27	34.92218127459021	35.0	34.0	39.0	29.0	41.0
28	34.9033261011741	36.0	34.0	40.0	27.0	41.0
29	34.932115977535325	36.0	34.0	40.0	28.0	41.0
30	34.502307716264056	36.0	33.0	40.0	25.0	41.0
31	34.24845996532716	35.0	33.0	40.0	24.0	41.0
32	33.83537563973466	35.0	33.0	40.0	23.0	41.0
33	33.60051490205442	35.0	33.0	40.0	21.0	41.0
34	33.378686584517645	35.0	33.0	40.0	18.0	41.0
35	33.12891341132992	35.0	33.0	40.0	16.0	41.0
36	32.84730039759574	35.0	33.0	40.0	15.0	41.0
37	32.75024551277393	35.0	32.0	40.0	15.0	41.0
38	32.663437801700425	35.0	32.0	40.0	14.0	41.0
39	32.52582088467647	35.0	32.0	40.0	12.0	41.0
40	32.42129265330274	35.0	31.0	40.0	12.0	41.0
41	32.29699052206501	35.0	31.0	40.0	10.0	41.0
42	32.276833559987125	35.0	31.0	40.0	10.0	41.0
43	32.23413821382969	35.0	31.0	39.0	10.0	41.0
44	32.087107724568924	35.0	31.0	39.0	10.0	41.0
45	32.054217317734015	35.0	31.0	39.0	10.0	40.0
46	31.929647354379263	35.0	31.0	39.0	10.0	40.0
47	31.985761297220982	35.0	31.0	39.0	10.0	40.0
48	31.864081429268445	35.0	31.0	39.0	10.0	40.0
49	31.920521338330097	35.0	31.0	39.0	10.0	40.0
50	31.786064425043342	35.0	31.0	39.0	10.0	40.0
51	30.402208057801907	34.0	28.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	7.0
8	38.0
9	108.0
10	145.0
11	120.0
12	99.0
13	120.0
14	162.0
15	335.0
16	589.0
17	1170.0
18	2127.0
19	3530.0
20	5305.0
21	7666.0
22	10731.0
23	15013.0
24	20439.0
25	26407.0
26	30304.0
27	30375.0
28	29260.0
29	30106.0
30	34534.0
31	43039.0
32	55896.0
33	76785.0
34	105665.0
35	92313.0
36	84852.0
37	100907.0
38	108215.0
39	46928.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.251824476533546	3.880347558886732	47.1556852038327	30.712142760747025
2	27.227003290805467	5.358303314681975	50.54095858983276	16.873734804679795
3	20.729894424316665	4.7214234550343095	53.39378587964164	21.154896241007382
4	16.579846152249065	4.157626467626571	56.67971223619056	22.58281514393381
5	16.04532383809652	5.0126130241152715	57.72353081626509	21.218532321523114
6	18.85268195455159	7.429538352936291	59.71763435725482	14.000145335257294
7	76.26862107984095	1.4570897652835595	19.47077204164893	2.803517113226546
8	78.7537501686927	3.2550945198226913	16.446552959129647	1.5446023523549501
9	75.14414143196753	3.724527400886545	16.840411506399942	4.290919660745985
10	45.29113766363193	24.15388927529612	21.436742829262215	9.118230231809736
11	39.00227345866769	18.045033167581934	31.165484952610324	11.78720842114005
12	35.56924705955631	18.416053317277246	31.965244111326808	14.049455511839634
13	19.97134819213321	31.106416551609588	34.08527027167312	14.83696498458408
14	15.450487392166428	34.206106157024365	35.62551256630921	14.717893884499993
15	12.722648423631513	20.367075335568728	53.147857862118364	13.762418378681392
16	15.321658067663943	17.795264146830135	51.187077619408484	15.696000166097438
17	14.999429040060624	16.628325841646856	38.83191977493797	29.540325343354546
18	18.682639703516074	19.715558139293464	42.41007380954853	19.191728347641934
19	23.495001505258024	21.402589043797818	35.19324398675373	19.90916546419043
20	27.560547706298205	19.88051365632364	36.49202213248347	16.066916504894685
21	22.364189392602434	23.07519023347071	36.75995806039718	17.800662313529674
22	23.065847252644584	20.354098973310215	34.15461595158259	22.4254378224626
23	18.82392633578673	25.276292705208192	33.934640658576335	21.96514030042874
24	18.79901172025039	20.006228653884083	44.37760176063283	16.817157865232694
25	16.401291407571968	21.835584299639777	42.23546387899802	19.52766041379024
26	16.22024520134124	29.4315315221792	35.48038493081004	18.867838345669526
27	16.888268330409325	29.86701823957479	37.313581579794246	15.931131850221636
28	14.715194801150226	25.76544965690498	43.546284088903654	15.97307145304114
29	15.851924134995693	21.918529207196173	42.68122787530235	19.54831878250579
30	17.542796042728565	27.55598002678321	37.117171360649444	17.78405256983878
31	22.66119237197521	26.072626104288428	32.79126742725451	18.474914096481847
32	25.015831161955383	25.27338600006229	33.235578070985895	16.47520476699644
33	24.23984469889649	27.252021717239877	30.152809641956214	18.35532394190742
34	19.72926117783845	28.08572703962462	31.93669611435809	20.248315668178847
35	19.470460608954728	24.74571520518224	34.09731233584902	21.686511850014014
36	26.446656770027715	25.10832667213404	30.221324834681145	18.2236917231571
37	19.62970652659116	29.56980763840588	32.14888559000924	18.65160024499372
38	20.489987438881332	31.04755577240499	27.853086817054056	20.609369971659625
39	20.052113070830178	28.224210777647436	30.968867111669386	20.754809039853004
40	21.65754860945302	26.499288895348233	29.74597473242741	22.097187762771338
41	16.767224823261945	25.069501396256577	32.793655077910074	25.369618702571394
42	20.15073342399485	26.12027530650168	30.1200053981667	23.608985871336774
43	22.414018623675112	26.21017554422863	29.296058300200357	22.079747531895897
44	20.04982923107268	29.862346749161727	30.218625751331373	19.86919826843422
45	18.382937640793532	33.6088820604387	26.584829075356332	21.423351223411434
46	22.180444103021934	31.376013453892387	27.97765989473575	18.465882548349928
47	20.094675539038086	29.750957655534677	29.611331997633112	20.543034807794122
48	22.015592396889826	25.722575756002865	31.980192880648612	20.281638966458697
49	20.665012613024114	24.180464865201547	33.46686875188157	21.687653769892766
50	19.572714343551787	30.867859107849142	29.641748590767058	19.917677957832012
51	17.73443096056224	31.060116891071228	26.502714654984484	24.702737493382052
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	159.0
1	591.0
2	1023.0
3	9923.0
4	18823.0
5	16982.5
6	15142.0
7	14796.5
8	14451.0
9	14832.5
10	15214.0
11	14964.0
12	14714.0
13	13739.0
14	12764.0
15	11856.0
16	10948.0
17	9889.0
18	8830.0
19	8282.5
20	7735.0
21	7487.5
22	7240.0
23	7709.0
24	8178.0
25	8707.5
26	10258.0
27	11279.0
28	12012.5
29	12746.0
30	16070.0
31	19394.0
32	19959.0
33	20524.0
34	22741.5
35	24959.0
36	26327.5
37	27696.0
38	31677.5
39	35659.0
40	46731.5
41	57804.0
42	66069.0
43	74334.0
44	75662.0
45	76990.0
46	82009.5
47	87029.0
48	74407.5
49	61786.0
50	60196.5
51	58607.0
52	53953.0
53	49299.0
54	44327.0
55	39355.0
56	38786.0
57	38217.0
58	36051.5
59	33886.0
60	31431.0
61	28976.0
62	24819.5
63	20663.0
64	17552.0
65	14441.0
66	11800.0
67	9159.0
68	7303.0
69	5447.0
70	4694.0
71	3941.0
72	3270.5
73	2600.0
74	2425.0
75	1510.5
76	771.0
77	559.5
78	348.0
79	348.0
80	348.0
81	226.0
82	104.0
83	115.5
84	127.0
85	87.0
86	47.0
87	38.5
88	30.0
89	21.5
90	13.0
91	7.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	963290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.34302874933737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.06174786632882	22.71159759879241
2	7.625075389990397	4.169853112087446
3	2.6134086061007262	2.143755199511336
4	1.2691551241208054	1.3881018018481608
5	0.783454768518766	1.0711013129707039
6	0.5572542315672612	0.9142211084660117
7	0.40287525444300826	0.7711080765242249
8	0.3213091348855762	0.7028451930080823
9	0.2594391742289238	0.638446751968123
>10	1.6490387576214312	9.132161741702214
>50	0.5562610009849995	11.43906062558965
>100	0.8890139382412336	41.18758459448931
>500	0.009264582943026317	1.6611574265229327
>1k	0.0023161457357565793	1.4163852780856039
>5k	3.8602428929276314E-4	0.6526201784338094
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6183	0.6418627827549336	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGC	4287	0.44503732001785545	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCG	3191	0.3312605757352407	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTC	2433	0.25257191499963666	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1431	0.14855339513542132	No Hit
GTTTTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC	1064	0.11045479554443625	No Hit
TTGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1013	0.10516043974296421	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.034672839954738445	0.0
2	0.0	0.0	0.0	0.1596611612287058	0.0
3	0.0	0.0	0.0	0.18883202358583603	0.0
4	0.0	0.0	0.0	0.31008315252935253	0.0
5	0.0	0.0	0.0	0.7282334499475756	0.0
6	0.0	0.0	0.0	0.7815922515545682	0.0
7	0.0	0.0	0.0	0.8910089381183237	0.0
8	0.0	0.0	0.0	1.0326070030831838	0.0
9	0.0	0.0	0.0	1.0819171796655214	0.0
10	0.0	0.0	0.0	1.5767837307560548	0.0
11	0.0	0.0	0.0	1.6696944845269857	0.0
12	0.0	0.0	0.0	2.3659541778695927	0.0
13	0.0	0.0	0.0	2.449106707222124	0.0
14	0.0	0.0	0.0	2.488139604895722	0.0
15	0.0	0.0	0.0	2.5869675798565335	0.0
16	0.0	0.0	0.0	2.6649295643056607	0.0
17	0.0	0.0	0.0	2.7466287410852392	0.0
18	0.0	0.0	0.0	2.8488824756822972	0.0
19	0.0	0.0	0.0	2.9597525148190056	0.0
20	0.0	0.0	0.0	3.025672435092236	0.0
21	0.0	0.0	0.0	3.0893085156079687	0.0
22	0.0	0.0	0.0	3.154397948696654	0.0
23	0.0	0.0	0.0	3.214400647780004	0.0
24	0.0	0.0	0.0	3.2675518275908604	0.0
25	0.0	0.0	0.0	3.3055466162837774	0.0
26	0.0	0.0	0.0	3.3421918633018093	0.0
27	0.0	0.0	0.0	3.3855848186942663	0.0
28	0.0	0.0	0.0	3.4286663413925194	0.0
29	0.0	0.0	0.0	3.473824082052134	0.0
30	1.0381089806807918E-4	0.0	0.0	3.533100104849007	0.0
31	1.0381089806807918E-4	0.0	0.0	3.596217130874399	0.0
32	1.0381089806807918E-4	0.0	0.0	3.6553893427732045	0.0
33	1.0381089806807918E-4	0.0	0.0	3.703453788578725	0.0
34	1.0381089806807918E-4	0.0	0.0	3.7561897247973093	0.0
35	1.0381089806807918E-4	0.0	0.0	3.822421077764744	0.0
36	1.0381089806807918E-4	0.0	0.0	3.8826313986442296	0.0
37	1.0381089806807918E-4	0.0	0.0	3.94367220670826	0.0
38	1.0381089806807918E-4	0.0	0.0	4.0054396910587675	0.0
39	1.0381089806807918E-4	0.0	0.0	4.0666881209189345	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGT	50	2.1827873E-11	45.0	29
TCTGCGA	25	3.8903334E-5	45.0	26
TATCATC	25	3.8903334E-5	45.0	11
TGTCACG	20	7.032718E-4	45.0	32
AGCGTCC	25	3.8903334E-5	45.0	37
AGCCGAG	25	3.8903334E-5	45.0	13
GTACCGT	20	7.032718E-4	45.0	38
GTACCCG	20	7.032718E-4	45.0	24
CGAACTA	25	3.8903334E-5	45.0	28
ACACGGT	20	7.032718E-4	45.0	40
CGTATAA	20	7.032718E-4	45.0	28
CACGGTG	20	7.032718E-4	45.0	4
CACGGAC	20	7.032718E-4	45.0	30
ACGTTGA	20	7.032718E-4	45.0	25
CGACGAT	25	3.8903334E-5	45.0	20
TCCGATC	35	1.211647E-7	45.0	18
CGTGATT	25	3.8903334E-5	45.0	36
CATGACG	20	7.032718E-4	45.0	14
CGGTTGA	20	7.032718E-4	45.0	34
CTATACG	20	7.032718E-4	45.0	10
>>END_MODULE