FastQCFastQC Report
Sat 18 Jun 2016
SRR3548162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058792
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42770.4039509176495478No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC23840.2251622603873093No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG16410.1549879485300229No Hit
GTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA13760.129959425458447No Hit
TTTTCTGGGTGTGATGGCATTTAAAACCCGCATGTTCTTTTTAAACTGAAT11870.1121088939092853No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC11510.10870879266182594No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA11130.10511979690061883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGT850.045.00000436
CTTAACG207.0330343E-445.034
TGTCACG253.890596E-545.01
AACCGGT207.0330343E-445.013
AGCCGGT207.0330343E-445.028
GTCGCGA207.0330343E-445.014
GGTACGA207.0330343E-445.023
GTACCGC351.2118107E-745.025
GTACCGA406.8139343E-945.09
ACCGGAG253.890596E-545.017
CGAACAT207.0330343E-445.011
AATTTCG406.8139343E-945.033
ACGCCAA406.8139343E-945.043
TCCGCAA207.0330343E-445.012
GTATTAC207.0330343E-445.019
CACGGTA406.8139343E-945.042
CAAGTCG207.0330343E-445.034
GTCGAAA253.890596E-545.015
CGAAATA207.0330343E-445.022
GGTCGGA351.2118107E-745.026