##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548162_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1058792
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.798511889020695	30.0	25.0	31.0	16.0	33.0
2	28.65445809941896	31.0	28.0	33.0	16.0	34.0
3	26.77940804237282	31.0	25.0	33.0	16.0	34.0
4	30.565856183273013	33.0	28.0	35.0	19.0	37.0
5	32.545768196208506	35.0	32.0	35.0	28.0	37.0
6	33.782105456029136	35.0	33.0	37.0	30.0	37.0
7	33.4341645951235	35.0	32.0	36.0	29.0	37.0
8	33.64840308578078	35.0	35.0	37.0	29.0	37.0
9	35.38614855420139	37.0	35.0	39.0	30.0	39.0
10	35.661387694655794	37.0	35.0	39.0	30.0	39.0
11	35.66510702763149	37.0	35.0	39.0	30.0	39.0
12	34.359320810886366	35.0	33.0	39.0	27.0	39.0
13	34.139134976463744	35.0	33.0	39.0	25.0	39.0
14	34.842332582792466	37.0	33.0	40.0	25.0	41.0
15	35.837922840369025	37.0	34.0	40.0	30.0	41.0
16	35.49758215022403	37.0	34.0	40.0	30.0	41.0
17	35.578427113162924	36.0	34.0	40.0	30.0	41.0
18	35.227330769405135	36.0	34.0	39.0	29.0	40.0
19	34.60033132097711	36.0	33.0	39.0	27.0	40.0
20	35.03541299896486	35.0	34.0	39.0	29.0	40.0
21	35.05500797134848	35.0	34.0	39.0	29.0	40.0
22	35.230422972595186	35.0	34.0	39.0	29.0	41.0
23	35.514562822537386	36.0	34.0	40.0	30.0	41.0
24	35.37817437230353	35.0	34.0	40.0	30.0	41.0
25	34.59716072656386	35.0	33.0	39.0	27.0	41.0
26	34.61859175362111	35.0	33.0	39.0	27.0	41.0
27	34.8828854014764	35.0	34.0	39.0	29.0	41.0
28	34.853346077416525	35.0	34.0	40.0	27.0	41.0
29	34.92666926081799	36.0	34.0	40.0	29.0	41.0
30	34.55827584643632	36.0	34.0	40.0	26.0	41.0
31	34.384962296655054	35.0	33.0	39.0	25.0	41.0
32	34.06616408133042	35.0	33.0	40.0	23.0	41.0
33	33.97000732910713	35.0	33.0	40.0	23.0	41.0
34	33.82591481613008	35.0	33.0	40.0	22.0	41.0
35	33.65721123695683	35.0	33.0	40.0	20.0	41.0
36	33.38334819303508	35.0	33.0	40.0	18.0	41.0
37	33.29538946270844	35.0	33.0	40.0	18.0	41.0
38	33.16073034174796	35.0	33.0	40.0	18.0	41.0
39	33.05308974756137	35.0	33.0	40.0	18.0	41.0
40	32.90943830327392	35.0	32.0	40.0	18.0	41.0
41	32.83849896863595	35.0	32.0	40.0	17.0	41.0
42	32.85739880920899	35.0	32.0	39.0	17.0	41.0
43	32.79767414185223	35.0	32.0	39.0	17.0	41.0
44	32.63702785816289	35.0	32.0	39.0	16.0	41.0
45	32.54858366893592	35.0	32.0	39.0	17.0	40.0
46	32.46416954415976	35.0	32.0	39.0	16.0	40.0
47	32.55351570468987	35.0	32.0	39.0	17.0	40.0
48	32.43086555244089	35.0	32.0	39.0	15.0	40.0
49	32.481894460857276	35.0	32.0	39.0	15.0	40.0
50	32.29818699045705	35.0	32.0	39.0	15.0	40.0
51	30.909861426984715	34.0	29.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	65.0
9	132.0
10	192.0
11	169.0
12	153.0
13	163.0
14	230.0
15	359.0
16	604.0
17	1232.0
18	2097.0
19	3526.0
20	5191.0
21	7448.0
22	10521.0
23	14344.0
24	19495.0
25	25394.0
26	28906.0
27	29673.0
28	30200.0
29	32537.0
30	38474.0
31	48724.0
32	65686.0
33	90467.0
34	127391.0
35	105161.0
36	92274.0
37	109471.0
38	117551.0
39	50958.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.72076857399754	3.8991605527809052	48.16932882001375	29.210742053207806
2	23.156767334849526	4.22349243288578	53.392073230625094	19.227667001639603
3	22.706442814074908	3.995307860278506	52.949587832171005	20.348661493475582
4	17.458858774905742	4.1111946444627465	55.492391328986244	22.93755525164527
5	16.463856923739506	5.325597473346984	55.210182925447114	23.000362677466395
6	19.57910524446728	6.229741063400555	58.66903036668203	15.522123325450135
7	80.85176314139132	1.7437797036622869	14.94476724418016	2.459689910766232
8	83.93036592645203	2.4031160038987824	12.261804018164096	1.4047140514850887
9	80.5033472107836	3.2653250119003543	12.926523812042404	3.3048039652736323
10	48.909039735849916	23.188690507672895	18.07852722725521	9.823742529221981
11	43.32333451707229	18.0478318687712	27.02051016630273	11.60832344785378
12	38.627416905303406	20.186495553423146	28.109203696287842	13.076883844985607
13	20.81796991288185	35.5939599090284	30.21056071447461	13.377509463615139
14	14.958839885454367	37.84322133147965	32.5666419844502	14.63129679861578
15	14.303470370006574	22.325725921616332	50.64979712729224	12.72100658108486
16	16.06188939848431	16.856285276050443	50.1442209612464	16.937604364218846
17	17.25900837936063	16.93968220387007	34.77340214130821	31.027907275461093
18	20.432719552093328	20.179223114643857	38.91699219487869	20.47106513838412
19	25.19928371200387	21.897596506207073	32.940842016184476	19.962277765604576
20	29.424570642770252	21.442832964359383	31.726911423584614	17.40568496928575
21	23.22250262563374	23.8118535085267	34.76121844517148	18.204425420668084
22	22.872764433429797	19.84988552992467	33.75781078814347	23.519539248502067
23	19.90268154651716	27.005493052459784	30.817006550861738	22.274818850161317
24	20.003173427830962	22.088002176064798	41.076056487015386	16.832767909088847
25	18.06634353111848	22.913754542913058	39.52853818313701	19.491363742831453
26	16.91937604364219	31.181478515138007	33.219744765733026	18.679400675486782
27	18.582875578961687	30.599588965538082	33.950860981193664	16.866674474306567
28	15.720934801169633	26.413592093631234	41.563309885227696	16.302163219971437
29	15.23292582490234	21.818355257689895	41.93477094651263	21.013947970895135
30	17.39463464023151	26.403014000861358	37.6366651806965	18.56568617821064
31	25.772484113971394	24.91310852367604	30.87660276994915	18.437804592403417
32	25.869481446780863	25.682003641886226	32.30662868627643	16.141886225056478
33	24.144591194493348	26.812631753923338	30.506936206544815	18.535840845038496
34	21.07307195369818	26.86882787176329	31.572112369568337	20.485987804970193
35	18.662966852790728	26.673038708263757	32.9917490876395	21.672245351306017
36	27.2136548066098	22.779922779922778	31.127454684206153	18.87896772926127
37	20.123877022115767	28.206012134583563	33.205388782688196	18.46472206061247
38	20.58279624326591	29.761369560782473	28.082947358876908	21.572886837074705
39	20.792752495296526	27.69061345382285	31.328155105063125	20.188478945817497
40	22.78795079675706	26.251520600835672	28.865631776590682	22.09489682581659
41	17.84864260402421	25.689559422436137	30.71755358937355	25.744244384166105
42	22.57024986966279	24.724591798955792	28.3139653491904	24.391192982191026
43	23.626736885053912	25.530321347346785	27.415677489063007	23.427264278536295
44	19.394838646306354	31.178456202918042	29.98058164398673	19.44612350678887
45	17.78857414865243	33.9059040869217	27.132619060212015	21.172902704213858
46	22.302869685452855	30.447528881971152	28.24719113858057	19.00241029399542
47	21.752903308676302	27.63970638236783	29.892084564295914	20.71530574465995
48	23.882877845695848	24.84142305570877	30.643412492727563	20.63228660586782
49	22.467019017899645	22.93878306598463	32.53254652471874	22.06165139139699
50	19.308702748037383	31.61801373640904	29.399353225184928	19.673930290368645
51	19.24778426735374	31.643042259480612	26.36986301369863	22.739310459467017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	104.0
1	515.0
2	926.0
3	7101.0
4	13276.0
5	12287.0
6	11298.0
7	11379.5
8	11461.0
9	11703.0
10	11945.0
11	11928.0
12	11911.0
13	11146.0
14	10381.0
15	9814.5
16	9248.0
17	8402.0
18	7556.0
19	7315.0
20	7074.0
21	6742.0
22	6410.0
23	7115.0
24	7820.0
25	7986.5
26	9087.5
27	10022.0
28	11821.0
29	13620.0
30	14589.0
31	15558.0
32	19094.5
33	22631.0
34	25092.0
35	27553.0
36	30329.0
37	33105.0
38	36634.5
39	40164.0
40	52686.0
41	65208.0
42	79251.0
43	93294.0
44	95412.0
45	97530.0
46	97693.0
47	97856.0
48	86681.0
49	75506.0
50	70602.0
51	65698.0
52	63337.5
53	60977.0
54	55041.0
55	49105.0
56	45642.0
57	42179.0
58	40293.5
59	38408.0
60	34286.5
61	30165.0
62	25959.0
63	21753.0
64	18251.0
65	14749.0
66	12413.5
67	10078.0
68	7978.5
69	5879.0
70	4646.5
71	3414.0
72	3335.5
73	3257.0
74	2465.5
75	1285.0
76	896.0
77	643.0
78	390.0
79	375.5
80	361.0
81	228.0
82	95.0
83	63.0
84	31.0
85	26.0
86	21.0
87	18.0
88	15.0
89	14.0
90	13.0
91	13.0
92	13.0
93	9.5
94	6.0
95	4.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1058792.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.344583079434724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.54968343638383	20.096376265980822
2	7.88091935919375	3.837153921644354
3	2.648181094387848	1.9340659398514002
4	1.2788369570962945	1.2453101018832897
5	0.8157377655086879	0.9929397901729347
6	0.5402491637051219	0.7891284389648682
7	0.40639511417566765	0.6925463734088131
8	0.33790362433272886	0.6580898284328175
9	0.2750224859803314	0.6025776982794765
>10	1.7724609378278016	8.406841128177005
>50	0.4076209258856649	7.285185505443067
>100	1.0712356697900822	50.00655108589043
>500	0.012208935942460496	1.996848790593387
>1k	0.003544529789746596	1.4563851312773586
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4277	0.4039509176495478	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	2384	0.2251622603873093	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	1641	0.1549879485300229	No Hit
GTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1376	0.129959425458447	No Hit
TTTTCTGGGTGTGATGGCATTTAAAACCCGCATGTTCTTTTTAAACTGAAT	1187	0.1121088939092853	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC	1151	0.10870879266182594	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1113	0.10511979690061883	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01728384800791846	0.0
2	0.0	0.0	0.0	0.09303054802076328	0.0
3	0.0	0.0	0.0	0.10899213443244754	0.0
4	0.0	0.0	0.0	0.184266598160923	0.0
5	0.0	0.0	0.0	0.4098066475757278	0.0
6	0.0	0.0	0.0	0.44012421703224053	0.0
7	0.0	0.0	0.0	0.5007593559452659	0.0
8	0.0	0.0	0.0	0.5793394736643269	0.0
9	0.0	0.0	0.0	0.6065402836440018	0.0
10	0.0	0.0	0.0	0.8795873032663639	0.0
11	0.0	0.0	0.0	0.9446614632524613	0.0
12	0.0	0.0	0.0	1.345684515938919	0.0
13	0.0	0.0	0.0	1.39498598402708	0.0
14	0.0	0.0	0.0	1.4195422708142864	0.0
15	0.0	0.0	0.0	1.476399519452357	0.0
16	0.0	0.0	0.0	1.523434253375545	0.0
17	0.0	0.0	0.0	1.571885696151841	0.0
18	0.0	0.0	0.0	1.6351653582573347	0.0
19	0.0	0.0	0.0	1.713651028719522	0.0
20	0.0	0.0	0.0	1.7570023196246287	0.0
21	0.0	0.0	0.0	1.7974257455666458	0.0
22	0.0	0.0	0.0	1.8459716355998157	0.0
23	0.0	0.0	0.0	1.8896062682755441	0.0
24	0.0	0.0	0.0	1.9327686646669033	0.0
25	0.0	0.0	0.0	1.9640307066921547	0.0
26	0.0	0.0	0.0	1.9927426727818118	0.0
27	0.0	0.0	0.0	2.0296715502194953	0.0
28	0.0	0.0	0.0	2.0566834656854227	0.0
29	0.0	0.0	0.0	2.0965402080861963	0.0
30	0.0	0.0	0.0	2.137152528541961	0.0
31	0.0	0.0	0.0	2.179276005107708	0.0
32	0.0	0.0	0.0	2.2155437517472745	0.0
33	0.0	0.0	0.0	2.253889338038066	0.0
34	0.0	0.0	0.0	2.3006407301906324	0.0
35	0.0	0.0	0.0	2.352397826957514	0.0
36	0.0	0.0	0.0	2.397071379458855	0.0
37	9.444725687387136E-5	0.0	0.0	2.441178248418953	0.0
38	9.444725687387136E-5	0.0	0.0	2.4842461975534382	0.0
39	9.444725687387136E-5	0.0	0.0	2.5312809314766263	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGGT	85	0.0	45.000004	36
CTTAACG	20	7.0330343E-4	45.0	34
TGTCACG	25	3.890596E-5	45.0	1
AACCGGT	20	7.0330343E-4	45.0	13
AGCCGGT	20	7.0330343E-4	45.0	28
GTCGCGA	20	7.0330343E-4	45.0	14
GGTACGA	20	7.0330343E-4	45.0	23
GTACCGC	35	1.2118107E-7	45.0	25
GTACCGA	40	6.8139343E-9	45.0	9
ACCGGAG	25	3.890596E-5	45.0	17
CGAACAT	20	7.0330343E-4	45.0	11
AATTTCG	40	6.8139343E-9	45.0	33
ACGCCAA	40	6.8139343E-9	45.0	43
TCCGCAA	20	7.0330343E-4	45.0	12
GTATTAC	20	7.0330343E-4	45.0	19
CACGGTA	40	6.8139343E-9	45.0	42
CAAGTCG	20	7.0330343E-4	45.0	34
GTCGAAA	25	3.890596E-5	45.0	15
CGAAATA	20	7.0330343E-4	45.0	22
GGTCGGA	35	1.2118107E-7	45.0	26
>>END_MODULE