SRR3548160_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548160_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	973384
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC	30404	3.123536035110501	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG	22135	2.2740254616882956	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	19485	2.0017793594306053	No Hit
GAACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	5232	0.5375062667970708	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTC	4523	0.46466759264586227	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4272	0.4388812637150395	No Hit
GATTGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC	4204	0.4318953259967289	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTA	3881	0.3987121218347538	No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	3832	0.39367813730244183	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT	3831	0.3935754029242313	No Hit
GATTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	3316	0.34066719814584995	No Hit
GATTGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG	3276	0.336557823017432	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATTATGCT	3000	0.30820313463134796	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2560	0.26300000821875025	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1806	0.18553828704807146	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGATTATGCTCGT	1527	0.15687539552735613	No Hit
GTATGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC	1483	0.15235508288609634	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTAT	1238	0.12718516022453627	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTT	1125	0.11557617548675549	No Hit
GTATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG	1122	0.11526797235212413	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTT	1030	0.10581640955676279	No Hit
TTCTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	985	0.10119336253729258	No Hit
GTATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	985	0.10119336253729258	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTAAC	30	2.1651576E-6	45.000004	14
TCCATAC	55	1.8189894E-12	45.000004	31
CATATGG	30	2.1651576E-6	45.000004	33
ATAGGTG	30	2.1651576E-6	45.000004	13
GTCGACT	30	2.1651576E-6	45.000004	35
ACGTTGC	30	2.1651576E-6	45.000004	23
ACGTTAT	30	2.1651576E-6	45.000004	39
GACGTAT	30	2.1651576E-6	45.000004	21
GACGTAC	30	2.1651576E-6	45.000004	21
CGTTGCA	30	2.1651576E-6	45.000004	24
GCACGGT	35	1.2116652E-7	45.000004	31
ACACAAG	35	1.2116652E-7	45.000004	35
TATATCG	30	2.1651576E-6	45.000004	11
TTCGTGA	35	1.2116652E-7	45.000004	16
CTCCACG	30	2.1651576E-6	45.000004	26
TGACGTA	30	2.1651576E-6	45.000004	20
CGTTCTA	30	2.1651576E-6	45.000004	28
CGTTATT	30	2.1651576E-6	45.000004	40
TAACCGT	35	1.2116652E-7	45.000004	38
TCGTGAT	35	1.2116652E-7	45.000004	17

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content