##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548160_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	973384
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.58757797539306	30.0	25.0	31.0	16.0	33.0
2	28.1142591207581	31.0	27.0	31.0	16.0	34.0
3	26.165574942674215	30.0	22.0	31.0	10.0	34.0
4	30.70672930724154	33.0	28.0	35.0	19.0	37.0
5	32.517290195852816	35.0	32.0	35.0	28.0	37.0
6	33.589355280136104	35.0	33.0	36.0	29.0	37.0
7	33.45645089707659	35.0	32.0	35.0	29.0	37.0
8	33.385146047192066	35.0	33.0	37.0	28.0	37.0
9	35.246398132699944	37.0	35.0	39.0	30.0	39.0
10	35.42217048975533	37.0	35.0	39.0	30.0	39.0
11	35.387734953522965	37.0	35.0	39.0	30.0	39.0
12	34.27623425081982	35.0	33.0	39.0	27.0	39.0
13	33.748513433547295	35.0	32.0	39.0	25.0	39.0
14	34.704454768107965	37.0	33.0	39.0	25.0	41.0
15	35.36523201531975	37.0	33.0	40.0	27.0	41.0
16	35.365942937216964	36.0	34.0	40.0	30.0	41.0
17	35.06947104123347	36.0	33.0	39.0	28.0	41.0
18	34.97004368265762	36.0	33.0	39.0	27.0	40.0
19	34.343258159164314	36.0	32.0	39.0	27.0	40.0
20	34.24620293738134	35.0	32.0	39.0	27.0	40.0
21	34.830481084546285	35.0	33.0	39.0	29.0	40.0
22	35.04438947013717	35.0	33.0	39.0	29.0	40.0
23	35.191667420052106	35.0	34.0	39.0	29.0	41.0
24	35.09656928817404	35.0	34.0	39.0	29.0	41.0
25	34.519066473252074	35.0	33.0	39.0	27.0	40.0
26	34.30870756042836	35.0	33.0	39.0	25.0	40.0
27	34.54400832559401	35.0	33.0	39.0	27.0	40.0
28	34.4237166421474	35.0	33.0	39.0	25.0	40.0
29	34.42360260698758	35.0	33.0	39.0	25.0	41.0
30	34.11795858571745	35.0	33.0	39.0	24.0	41.0
31	34.07661827192557	35.0	33.0	39.0	24.0	40.0
32	33.650425731263304	35.0	33.0	39.0	23.0	40.0
33	33.42450050545314	35.0	33.0	39.0	21.0	41.0
34	33.400645582832674	35.0	33.0	40.0	18.0	41.0
35	33.04268613414644	35.0	33.0	40.0	17.0	41.0
36	32.9624896238278	35.0	33.0	40.0	16.0	41.0
37	32.74848569526518	35.0	32.0	40.0	15.0	41.0
38	32.69890094762191	35.0	32.0	40.0	15.0	41.0
39	32.745055394376735	35.0	32.0	40.0	15.0	41.0
40	32.4055583407987	35.0	31.0	39.0	15.0	40.0
41	32.37127690613365	35.0	31.0	39.0	15.0	40.0
42	32.538552102767255	35.0	32.0	39.0	15.0	40.0
43	32.56994464671702	35.0	32.0	39.0	15.0	40.0
44	32.47006628422082	35.0	32.0	39.0	14.0	40.0
45	32.38061032439407	35.0	31.0	39.0	14.0	40.0
46	32.22423729997617	35.0	31.0	39.0	13.0	40.0
47	32.147401231168786	35.0	31.0	39.0	13.0	40.0
48	32.085538697985584	35.0	31.0	39.0	12.0	40.0
49	32.09118497941203	35.0	31.0	39.0	12.0	40.0
50	31.88638913316841	35.0	31.0	39.0	11.0	40.0
51	30.03175005958594	34.0	27.0	37.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	39.0
9	108.0
10	123.0
11	127.0
12	123.0
13	122.0
14	196.0
15	339.0
16	623.0
17	1154.0
18	2258.0
19	3954.0
20	5946.0
21	8447.0
22	11492.0
23	15218.0
24	19715.0
25	24717.0
26	27843.0
27	28071.0
28	28324.0
29	31353.0
30	37574.0
31	48698.0
32	64546.0
33	87246.0
34	118209.0
35	100860.0
36	87431.0
37	92769.0
38	89909.0
39	35846.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.46949199904662	3.277432133669754	40.297662587426956	24.955413279856664
2	23.12078275377446	18.163129864472808	42.89540407485637	15.820683306896353
3	17.18705053709533	17.056064204877007	46.75903857059496	18.99784668743271
4	14.953810623556581	3.5185497193296786	62.137039441782484	19.390600215331258
5	25.5171648599114	4.838378276199321	49.40167498130235	20.242781882586932
6	17.17081850533808	16.931755607242362	52.61541180048162	13.28201408693794
7	67.91554001298562	1.931611779112868	27.238068429314637	2.914779778586868
8	67.97502321796948	12.915663294239476	15.705415334544229	3.403898153246817
9	65.2283168821349	3.3045540095173123	15.384164933880154	16.08296417446763
10	49.65655897364247	17.346288823321526	21.10390144074692	11.893250762289085
11	44.77883343058854	15.615214550475454	27.17314030228564	12.432811716650367
12	36.40711168459724	15.304340322010635	29.279297789978052	19.00925020341407
13	20.81357408792419	31.10725058147658	36.351635120363596	11.727540210235631
14	15.981154405661075	37.372506636640836	30.5853599401675	16.060979017530595
15	11.240476523139892	19.65185373912043	55.343831416994725	13.763838320744949
16	13.062881658215053	17.6055903939247	45.460476030014874	23.871051917845374
17	13.811404337856386	17.343309526353423	39.725740303929385	29.119545831860805
18	16.730807163462725	19.98132289004134	39.38836060588627	23.89950934060967
19	20.881276043164874	22.38417726200554	32.8033951657311	23.931151529098486
20	25.67671134927223	19.902628356332137	38.650419567200615	15.770240727195024
21	20.83206627600207	28.364550886392216	33.56619792394368	17.23718491366203
22	20.755529164235288	18.930863872839495	35.34997493281172	24.9636320301135
23	18.402911903215998	29.329226697788336	31.24666113270816	21.021200266287508
24	19.964577186393036	20.002794375087323	37.876624230519504	22.156004208000134
25	14.133476613546145	28.982600905706278	37.501438281294945	19.382484199452634
26	15.754625101707035	26.14528284829009	34.87554757423586	23.224544475767015
27	18.69159550598736	31.347032620219768	31.23248378851512	18.728888085277752
28	12.829263682164491	26.297843399932606	43.607456050233004	17.265436867669905
29	18.931788482243388	19.309850994057843	38.64600198893756	23.112358534761203
30	14.467877014621155	31.186458787076837	37.05844764245148	17.287216555850517
31	25.47350274917196	21.345635432676108	32.36266468320827	20.81819713494366
32	27.24053405439169	26.585807862056495	31.85669787052181	14.316960213030006
33	23.456107764253368	25.731982444749452	27.100815300025477	23.711094490971703
34	25.778623852456995	24.35564997986406	31.93487873233996	17.930847435338983
35	20.725325257041412	25.585380487043142	29.451172404724137	24.23812185119131
36	28.807233322101037	21.601238565663706	29.205329037666534	20.38619907456872
37	18.74738027335563	28.226064944564527	36.29369293105291	16.732861851026932
38	20.34284516696391	28.157952051811	28.748982929655714	22.75021985156937
39	21.080683471271357	24.653374207917945	35.858921042466285	18.407021278344416
40	21.97488349921511	28.347394245231072	27.598563362455103	22.079158893098715
41	15.071236017851126	24.31887107246472	33.3697697928053	27.240123116878845
42	23.36549604267175	22.481261249414413	31.998060374939385	22.155182332974448
43	23.835300354228135	23.14564447330139	26.165726989553967	26.85332818291651
44	21.252969023530284	26.773606305425197	30.659945098748288	21.313479572296238
45	17.409984137812003	30.21109860034683	26.06073245502289	26.318184806818273
46	27.898445012451408	29.187966927748967	25.187387505855856	17.726200553943766
47	18.375070886720966	25.726640257082504	36.29204918100154	19.60623967519499
48	23.91450855982839	25.52528087579003	27.717837975557437	22.842372588824144
49	21.020481125640035	20.63091236346601	35.78104838378276	22.56755812711119
50	22.5833792213556	25.787767212117725	29.00848996901531	22.62036359751136
51	19.130476769702398	26.948973889030437	25.657500020546877	28.26304932072029
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	100.0
1	360.5
2	621.0
3	6545.0
4	12469.0
5	11381.5
6	10294.0
7	10418.0
8	10542.0
9	10897.5
10	11253.0
11	11191.5
12	11130.0
13	10687.5
14	10245.0
15	9576.5
16	8908.0
17	8344.0
18	7780.0
19	7073.5
20	6367.0
21	5744.5
22	5122.0
23	5465.5
24	5809.0
25	5669.5
26	6753.5
27	7977.0
28	9122.5
29	10268.0
30	11089.5
31	11911.0
32	12126.0
33	12341.0
34	14811.5
35	17282.0
36	19097.5
37	20913.0
38	24772.5
39	28632.0
40	38794.5
41	48957.0
42	61820.5
43	74684.0
44	136314.0
45	197944.0
46	141463.5
47	84983.0
48	77168.0
49	69353.0
50	63149.0
51	56945.0
52	51369.0
53	45793.0
54	43916.0
55	42039.0
56	39019.0
57	35999.0
58	32594.0
59	29189.0
60	26038.5
61	22888.0
62	20070.5
63	17253.0
64	14516.0
65	11779.0
66	9561.5
67	7344.0
68	6051.0
69	4758.0
70	3960.5
71	3163.0
72	2635.5
73	2108.0
74	1737.0
75	911.5
76	457.0
77	371.5
78	286.0
79	252.5
80	219.0
81	152.0
82	85.0
83	101.0
84	117.0
85	82.5
86	48.0
87	40.5
88	33.0
89	31.0
90	29.0
91	28.0
92	27.0
93	26.5
94	26.0
95	20.5
96	15.0
97	9.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	973384.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.297236686991432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.53781830019314	20.297381435546768
2	7.478531098232898	3.6341528032958132
3	2.4069802660049455	1.7544890767211927
4	1.2520872954956606	1.2168904548573218
5	0.7469704922038242	0.9074659423637401
6	0.544813895950818	0.7942483308167368
7	0.37644835511868263	0.6402658349323065
8	0.2985929177808931	0.5803986237105386
9	0.2396877339181889	0.5241374641780975
>10	1.7581522853921692	8.642818774475966
>50	0.4851318095374106	8.822757158607773
>100	0.8532814805998032	37.1993691676251
>500	0.012472360351509218	2.1029156112648604
>1k	0.00731138365433299	4.809620051882154
>5k	4.300813914313524E-4	0.5467329794343943
>10k+	0.0012902441742940571	7.526356290287235
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC	30404	3.123536035110501	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG	22135	2.2740254616882956	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	19485	2.0017793594306053	No Hit
GAACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	5232	0.5375062667970708	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTC	4523	0.46466759264586227	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4272	0.4388812637150395	No Hit
GATTGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC	4204	0.4318953259967289	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTA	3881	0.3987121218347538	No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	3832	0.39367813730244183	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT	3831	0.3935754029242313	No Hit
GATTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	3316	0.34066719814584995	No Hit
GATTGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG	3276	0.336557823017432	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATTATGCT	3000	0.30820313463134796	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2560	0.26300000821875025	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1806	0.18553828704807146	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGATTATGCTCGT	1527	0.15687539552735613	No Hit
GTATGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC	1483	0.15235508288609634	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTAT	1238	0.12718516022453627	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTT	1125	0.11557617548675549	No Hit
GTATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG	1122	0.11526797235212413	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTT	1030	0.10581640955676279	No Hit
TTCTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	985	0.10119336253729258	No Hit
GTATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	985	0.10119336253729258	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0546875642089865E-4	0.0	0.0	0.07366054917689216	0.0
2	2.0546875642089865E-4	0.0	0.0	0.6175363474230109	0.0
3	2.0546875642089865E-4	0.0	0.0	0.6706500209578131	0.0
4	2.0546875642089865E-4	0.0	0.0	1.4468082483377578	0.0
5	2.0546875642089865E-4	0.0	0.0	4.337959119936222	0.0
6	2.0546875642089865E-4	0.0	0.0	4.552468501639641	0.0
7	2.0546875642089865E-4	0.0	0.0	5.132198597881207	0.0
8	2.0546875642089865E-4	0.0	0.0	5.800999400031231	0.0
9	2.0546875642089865E-4	0.0	0.0	5.88287869946496	0.0
10	2.0546875642089865E-4	0.0	0.0	8.91015262219227	0.0
11	2.0546875642089865E-4	0.0	0.0	9.014119812941244	0.0
12	2.0546875642089865E-4	0.0	0.0	13.191813302869166	0.0
13	2.0546875642089865E-4	0.0	0.0	13.418239872444996	0.0
14	2.0546875642089865E-4	0.0	0.0	13.61579808174369	0.0
15	2.0546875642089865E-4	0.0	0.0	14.222444585076394	0.0
16	2.0546875642089865E-4	0.0	0.0	14.503731312616603	0.0
17	2.0546875642089865E-4	0.0	0.0	14.601945378185793	0.0
18	3.0820313463134796E-4	0.0	0.0	14.666668036458375	0.0
19	3.0820313463134796E-4	0.0	0.0	15.176949693029679	0.0
20	3.0820313463134796E-4	0.0	0.0	15.269616102175503	0.0
21	3.0820313463134796E-4	0.0	0.0	15.318620400581887	0.0
22	3.0820313463134796E-4	0.0	0.0	15.42186845068339	0.0
23	3.0820313463134796E-4	0.0	0.0	15.46532509266641	0.0
24	3.0820313463134796E-4	0.0	0.0	15.509809078431534	0.0
25	3.0820313463134796E-4	0.0	0.0	15.5409375950293	0.0
26	3.0820313463134796E-4	0.0	0.0	15.590661034083157	0.0
27	3.0820313463134796E-4	0.0	0.0	15.674184083568253	0.0
28	3.0820313463134796E-4	0.0	0.0	15.705929006435282	0.0
29	3.0820313463134796E-4	0.0	0.0	15.7444543982642	0.0
30	3.0820313463134796E-4	0.0	0.0	15.78780830586901	0.0
31	3.0820313463134796E-4	0.0	0.0	15.830032135313504	0.0
32	3.0820313463134796E-4	0.0	0.0	15.880269246258415	0.0
33	3.0820313463134796E-4	0.0	0.0	15.91571260674102	0.0
34	3.0820313463134796E-4	0.0	0.0	15.992455187264225	0.0
35	3.0820313463134796E-4	0.0	0.0	16.038480188702504	0.0
36	3.0820313463134796E-4	0.0	0.0	16.081217690038052	0.0
37	3.0820313463134796E-4	0.0	0.0	16.134023160438225	0.0
38	3.0820313463134796E-4	0.0	0.0	16.173884099183876	0.0
39	3.0820313463134796E-4	0.0	0.0	16.217135272410477	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTAAC	30	2.1651576E-6	45.000004	14
TCCATAC	55	1.8189894E-12	45.000004	31
CATATGG	30	2.1651576E-6	45.000004	33
ATAGGTG	30	2.1651576E-6	45.000004	13
GTCGACT	30	2.1651576E-6	45.000004	35
ACGTTGC	30	2.1651576E-6	45.000004	23
ACGTTAT	30	2.1651576E-6	45.000004	39
GACGTAT	30	2.1651576E-6	45.000004	21
GACGTAC	30	2.1651576E-6	45.000004	21
CGTTGCA	30	2.1651576E-6	45.000004	24
GCACGGT	35	1.2116652E-7	45.000004	31
ACACAAG	35	1.2116652E-7	45.000004	35
TATATCG	30	2.1651576E-6	45.000004	11
TTCGTGA	35	1.2116652E-7	45.000004	16
CTCCACG	30	2.1651576E-6	45.000004	26
TGACGTA	30	2.1651576E-6	45.000004	20
CGTTCTA	30	2.1651576E-6	45.000004	28
CGTTATT	30	2.1651576E-6	45.000004	40
TAACCGT	35	1.2116652E-7	45.000004	38
TCGTGAT	35	1.2116652E-7	45.000004	17
>>END_MODULE