SRR3548159_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548159_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	819296
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC	17052	2.081299066515643	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG	12163	1.4845672382142718	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	10128	1.2361832597742453	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3097	0.37800746006327385	No Hit
GAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	2776	0.3388274811545522	TruSeq Adapter, Index 27 (100% over 22bp)
GATTGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC	2432	0.29684021403741745	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTC	2412	0.29439909385618873	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTA	2261	0.27596863648791153	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2199	0.2684011639261024	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT	2035	0.24838397844002655	No Hit
GATTGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG	1990	0.24289145803226184	No Hit
GATTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	1864	0.22751240089052063	TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	1762	0.21506268796625394	TruSeq Adapter, Index 27 (96% over 25bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACCTTTCT	1633	0.19931746279732843	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1537	0.18760008592743038	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1445	0.17637093309377808	No Hit
TGCTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1098	0.13401749794945905	No Hit
TTTTCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1026	0.1252294652970355	No Hit
TTTTTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC	910	0.11107096824590867	No Hit
GTATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC	905	0.11046068820060148	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGT	833	0.10167265554817795	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	30	2.1647356E-6	45.000004	1
CCCCTAA	30	2.1647356E-6	45.000004	32
GATCGAC	30	2.1647356E-6	45.000004	9
CCCTAAT	30	2.1647356E-6	45.000004	33
GCGCGAT	30	2.1647356E-6	45.000004	1
GCTATCG	30	2.1647356E-6	45.000004	33
GCCGGTC	30	2.1647356E-6	45.000004	9
CAATTGT	30	2.1647356E-6	45.000004	36
CGCGATT	30	2.1647356E-6	45.000004	2
TACGGGA	30	2.1647356E-6	45.000004	4
ATGTCAC	30	2.1647356E-6	45.000004	45
GGCTTCG	30	2.1647356E-6	45.000004	1
CGGATGA	30	2.1647356E-6	45.000004	19
TAATTCA	30	2.1647356E-6	45.000004	14
CGCTAGG	60	0.0	45.000004	2
CGCTAAG	30	2.1647356E-6	45.000004	40
GTCGTAA	30	2.1647356E-6	45.000004	24
CAGGATG	30	2.1647356E-6	45.000004	3
CGTACTC	30	2.1647356E-6	45.000004	23
TACTCTA	30	2.1647356E-6	45.000004	41

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content