##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548159_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	819296
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.660607399523492	30.0	25.0	31.0	16.0	33.0
2	28.18175848533375	31.0	27.0	33.0	16.0	34.0
3	26.294684704917394	30.0	22.0	31.0	10.0	34.0
4	30.67092967620982	33.0	28.0	35.0	19.0	37.0
5	32.511946842166935	35.0	32.0	35.0	28.0	37.0
6	33.5804727473343	35.0	33.0	36.0	28.0	37.0
7	33.34793652111081	35.0	32.0	35.0	28.0	37.0
8	33.361277242901224	35.0	33.0	37.0	28.0	37.0
9	35.20661763269929	37.0	35.0	39.0	30.0	39.0
10	35.35420775885638	37.0	34.0	39.0	30.0	39.0
11	35.34652921532633	37.0	35.0	39.0	30.0	39.0
12	34.111129555130255	35.0	33.0	38.0	26.0	39.0
13	33.60354499472718	35.0	32.0	39.0	24.0	39.0
14	34.44992774284263	36.0	33.0	39.0	25.0	41.0
15	35.24183567355388	36.0	33.0	40.0	27.0	41.0
16	35.147734152247786	36.0	34.0	39.0	29.0	41.0
17	35.01931048119361	36.0	34.0	39.0	29.0	41.0
18	34.81419169628559	36.0	33.0	39.0	27.0	40.0
19	34.15240157403429	36.0	32.0	39.0	26.0	40.0
20	34.296935417724484	35.0	33.0	39.0	27.0	40.0
21	34.73357614342069	35.0	33.0	39.0	29.0	40.0
22	34.994661270163654	35.0	33.0	39.0	29.0	40.0
23	35.20440841893528	35.0	34.0	39.0	30.0	41.0
24	35.09383910088661	35.0	34.0	39.0	29.0	41.0
25	34.4416608893489	35.0	33.0	39.0	27.0	40.0
26	34.28556345936023	35.0	33.0	39.0	25.0	40.0
27	34.534356325430615	35.0	33.0	39.0	27.0	40.0
28	34.44634784009686	35.0	33.0	39.0	26.0	40.0
29	34.47576822052103	35.0	33.0	39.0	27.0	41.0
30	34.205986603132445	35.0	33.0	39.0	25.0	41.0
31	34.20284732257939	35.0	33.0	39.0	25.0	40.0
32	33.748447447564736	35.0	33.0	39.0	23.0	40.0
33	33.61735197047221	35.0	33.0	39.0	23.0	41.0
34	33.59518293754638	35.0	33.0	39.0	22.0	41.0
35	33.41487935984065	35.0	33.0	40.0	20.0	41.0
36	33.231429178221305	35.0	33.0	40.0	18.0	41.0
37	33.08842591688474	35.0	33.0	40.0	18.0	41.0
38	33.04705381205328	35.0	32.0	40.0	18.0	41.0
39	32.80877729172363	35.0	32.0	39.0	18.0	40.0
40	32.48360787798305	35.0	31.0	39.0	15.0	40.0
41	32.3702922997305	35.0	31.0	39.0	15.0	40.0
42	32.560973079326644	35.0	32.0	39.0	15.0	40.0
43	32.53313088309964	35.0	32.0	39.0	15.0	40.0
44	32.37440802835605	35.0	31.0	39.0	15.0	40.0
45	32.35471185017381	35.0	31.0	39.0	15.0	40.0
46	32.04294784790845	35.0	31.0	38.0	15.0	40.0
47	32.03613346092255	35.0	31.0	38.0	15.0	40.0
48	31.934685388431042	35.0	31.0	38.0	14.0	40.0
49	32.03289775612233	35.0	31.0	38.0	14.0	40.0
50	31.83146994492833	35.0	31.0	38.0	13.0	40.0
51	30.024711459594577	34.0	27.0	37.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	35.0
9	87.0
10	141.0
11	116.0
12	113.0
13	111.0
14	178.0
15	268.0
16	551.0
17	928.0
18	1648.0
19	2749.0
20	4245.0
21	6256.0
22	8574.0
23	11648.0
24	15697.0
25	19511.0
26	22543.0
27	24173.0
28	25666.0
29	29041.0
30	34632.0
31	43334.0
32	57400.0
33	76931.0
34	103419.0
35	82921.0
36	67485.0
37	74406.0
38	74629.0
39	29858.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.71940299964848	3.4859196187946724	44.68507108541967	25.109606296137173
2	21.673363473030506	14.690295082607507	47.43609147365543	16.200249970706558
3	19.087240753036752	13.210610084755691	48.66958950123033	19.03255966097723
4	16.341834941217826	3.732106589071593	57.780582353630436	22.145476116080147
5	22.64053528883334	5.4079355934851385	50.707192516501976	21.24433660117955
6	17.404454556106703	14.162158731398664	54.64545170487833	13.787935007616294
7	72.90796000468696	2.452227278053353	21.86731047142913	2.772502245830567
8	73.73293656993322	10.03703179314924	13.417739132132953	2.8122925047845957
9	71.55667792836778	2.8114381127211656	13.176312346209428	12.455571612701636
10	51.92726438308011	17.308396476975354	19.241641604499474	11.522697535445065
11	47.64749248134984	17.303148068585713	23.18038901691208	11.868970433152365
12	39.653190055852825	16.813581416240282	27.6698531422099	15.86337538569699
13	21.173422059914852	33.78656798031481	33.08938405655587	11.950625903214467
14	15.927698902472368	40.240767683474594	28.377411826739053	15.454121587313988
15	11.977722337226107	19.784302620786626	54.55696109830879	13.681013943678474
16	13.157759832832088	16.31083271491622	48.20284732257939	22.328560129672304
17	14.930623364449477	15.751083857360465	36.710907901417805	32.60738487677226
18	17.120674335038863	20.160113072686794	38.93293754638128	23.78627504589306
19	23.545946764051088	21.385067179627388	31.473850720618678	23.595135335702846
20	28.0759334843573	18.82128070929188	35.47509080967074	17.627694996680077
21	20.574859391477563	27.525949107526458	33.55234249892591	18.34684900207007
22	20.25653731984533	19.012908643518337	33.322633089872284	27.40792094676405
23	18.84276256688669	29.32749580127329	29.955107799867204	21.874633831972815
24	20.80798636878491	19.29498008045932	39.30996855837206	20.587064992383706
25	15.971272897707301	26.525822169277035	38.37599597703394	19.12690895598172
26	15.216112369644183	28.557810608131863	33.341429715267736	22.884647306956214
27	17.920629418427527	32.342279225090806	31.090961020192946	18.646130336288717
28	12.322774674842792	27.55988067804554	42.788076592586805	17.32926805452486
29	16.39163379291489	20.574371167441317	38.171552161856035	24.86244287778776
30	15.109191305706362	30.240352693043782	36.03691461938054	18.61354138186931
31	25.318810295668477	23.950684490098816	27.877592469632468	22.852912744600243
32	27.569401046752333	26.089227824864274	29.099006952310276	17.242364176073117
33	24.673744287778774	23.9035708706011	28.17431062766082	23.248374213959302
34	21.314884974417062	25.37703101199078	30.203980002343478	23.104104011248683
35	18.132518650158186	25.095325743076984	32.19776491036207	24.574390696402766
36	25.401442213803072	21.998886849197362	26.952896144983008	25.646774792016558
37	18.612686989805884	28.904815841893527	33.033116236378554	19.449380931922043
38	19.342948092020467	28.07153946803109	26.757850642502834	25.82766179744561
39	21.19929793383588	26.03283794867789	29.703306253173455	23.064557864312775
40	19.753788618521266	23.84742510643284	33.57052884427606	22.82825743076983
41	15.542001913838222	24.635296644924424	31.039087216341837	28.78361422489552
42	20.280948521657617	23.248008045932117	33.07278443932351	23.39825899308675
43	22.677274147560833	23.355661445924305	26.39192672733664	27.57513767917822
44	19.744268249814475	27.382045072843024	30.64338163496465	22.230305042377847
45	17.228815959067298	31.700752841463892	25.628222278639225	25.44220892082959
46	25.439889856657423	31.092425692301685	25.554012225129867	17.913672225911025
47	19.453774948248252	25.493472444635394	34.12466312541499	20.928089481701363
48	23.54619087606921	23.721585361090497	29.570509315314613	23.16171444752568
49	21.364561770105066	19.275084950982308	36.26259618013514	23.09775709877749
50	20.83447252275124	27.88296293403117	28.597600867085887	22.6849636761317
51	18.751464672108735	29.838666367222594	23.998896613678085	27.410972346990587
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	236.0
2	463.0
3	4700.5
4	8938.0
5	8388.5
6	7839.0
7	7873.0
8	7907.0
9	8177.0
10	8447.0
11	8304.0
12	8161.0
13	7819.5
14	7478.0
15	6970.0
16	6462.0
17	5834.0
18	5206.0
19	5221.0
20	5236.0
21	4924.0
22	4612.0
23	4147.0
24	3682.0
25	4281.5
26	4604.5
27	4328.0
28	5091.5
29	5855.0
30	6994.5
31	8134.0
32	10334.0
33	12534.0
34	14081.5
35	15629.0
36	17168.5
37	18708.0
38	21652.5
39	24597.0
40	35261.5
41	45926.0
42	57678.0
43	69430.0
44	73928.0
45	78426.0
46	105855.5
47	133285.0
48	101795.0
49	70305.0
50	62240.5
51	54176.0
52	48618.0
53	43060.0
54	38546.5
55	34033.0
56	32408.0
57	30783.0
58	29479.0
59	28175.0
60	25423.5
61	22672.0
62	19723.5
63	16775.0
64	12734.5
65	8694.0
66	7108.5
67	5523.0
68	4369.0
69	3215.0
70	2681.0
71	2147.0
72	1623.5
73	1100.0
74	1046.0
75	731.0
76	470.0
77	474.0
78	478.0
79	382.5
80	287.0
81	215.5
82	144.0
83	97.5
84	51.0
85	40.5
86	30.0
87	17.5
88	5.0
89	6.0
90	7.0
91	4.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	819296.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.509338845527864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.60644379486041	20.246293217922865
2	7.770343746939219	3.8089197567992388
3	2.6138595496079673	1.9219190818788152
4	1.3103603172276503	1.2846426009866347
5	0.8028474983613796	0.9838630689311713
6	0.5559914602428124	0.8176189856586555
7	0.42205892279345736	0.724106960606633
8	0.3385343618893549	0.6637802709120603
9	0.2607072701382911	0.575078654098075
>10	1.8157472345544299	9.032849541849403
>50	0.5575874439241805	10.039804414870167
>100	0.922222284637534	39.10316702015654
>500	0.01418024380551947	2.244542044063748
>1k	0.0075965591815282865	3.669986983179386
>5k	0.0	0.0
>10k+	0.0015193118363056575	4.883427398086602
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC	17052	2.081299066515643	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG	12163	1.4845672382142718	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	10128	1.2361832597742453	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3097	0.37800746006327385	No Hit
GAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	2776	0.3388274811545522	TruSeq Adapter, Index 27 (100% over 22bp)
GATTGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC	2432	0.29684021403741745	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTC	2412	0.29439909385618873	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTA	2261	0.27596863648791153	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2199	0.2684011639261024	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT	2035	0.24838397844002655	No Hit
GATTGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG	1990	0.24289145803226184	No Hit
GATTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	1864	0.22751240089052063	TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	1762	0.21506268796625394	TruSeq Adapter, Index 27 (96% over 25bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACCTTTCT	1633	0.19931746279732843	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1537	0.18760008592743038	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1445	0.17637093309377808	No Hit
TGCTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1098	0.13401749794945905	No Hit
TTTTCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1026	0.1252294652970355	No Hit
TTTTTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC	910	0.11107096824590867	No Hit
GTATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC	905	0.11046068820060148	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGT	833	0.10167265554817795	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2205600906143811E-4	0.0	0.0	0.06786314103815959	0.0
2	1.2205600906143811E-4	0.0	0.0	0.4643010584697106	0.0
3	1.2205600906143811E-4	0.0	0.0	0.5012840292153263	0.0
4	1.2205600906143811E-4	0.0	0.0	1.109367066359411	0.0
5	1.2205600906143811E-4	0.0	0.0	3.296000468695075	0.0
6	1.2205600906143811E-4	0.0	0.0	3.4009686364879115	0.0
7	1.2205600906143811E-4	0.0	0.0	3.854650822169277	0.0
8	1.2205600906143811E-4	0.0	0.0	4.375097644807249	0.0
9	1.2205600906143811E-4	0.0	0.0	4.44479162598133	0.0
10	1.2205600906143811E-4	0.0	0.0	6.69050013670273	0.0
11	1.2205600906143811E-4	0.0	0.0	6.794613912432137	0.0
12	1.2205600906143811E-4	0.0	0.0	9.87237823692536	0.0
13	1.2205600906143811E-4	0.0	0.0	10.079873452329805	0.0
14	1.2205600906143811E-4	0.0	0.0	10.237935984064368	0.0
15	1.2205600906143811E-4	0.0	0.0	10.702725266570324	0.0
16	1.2205600906143811E-4	0.0	0.0	10.91791001054564	0.0
17	1.2205600906143811E-4	0.0	0.0	11.006522673124243	0.0
18	1.2205600906143811E-4	0.0	0.0	11.084028238878256	0.0
19	1.2205600906143811E-4	0.0	0.0	11.440431785337656	0.0
20	1.2205600906143811E-4	0.0	0.0	11.516106510955748	0.0
21	1.2205600906143811E-4	0.0	0.0	11.569078818888412	0.0
22	1.2205600906143811E-4	0.0	0.0	11.659644377611999	0.0
23	1.2205600906143811E-4	0.0	0.0	11.71737686989806	0.0
24	1.2205600906143811E-4	0.0	0.0	11.762659649259852	0.0
25	1.2205600906143811E-4	0.0	0.0	11.797811779869546	0.0
26	1.2205600906143811E-4	0.0	0.0	11.83979904698668	0.0
27	1.2205600906143811E-4	0.0	0.0	11.905343123852674	0.0
28	1.2205600906143811E-4	0.0	0.0	11.939152638362692	0.0
29	1.2205600906143811E-4	0.0	0.0	11.980407569425457	0.0
30	2.4411201812287622E-4	0.0	0.0	12.02739913291411	0.0
31	2.4411201812287622E-4	0.0	0.0	12.078906768738038	0.0
32	2.4411201812287622E-4	0.0	0.0	12.124189548099832	0.0
33	2.4411201812287622E-4	0.0	0.0	12.165810647189783	0.0
34	2.4411201812287622E-4	0.0	0.0	12.225496035620825	0.0
35	2.4411201812287622E-4	0.0	0.0	12.285913760106238	0.0
36	2.4411201812287622E-4	0.0	0.0	12.3404727961567	0.0
37	2.4411201812287622E-4	0.0	0.0	12.38624379955474	0.0
38	2.4411201812287622E-4	0.0	0.0	12.4338456430887	0.0
39	2.4411201812287622E-4	0.0	0.0	12.482790102722337	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	30	2.1647356E-6	45.000004	1
CCCCTAA	30	2.1647356E-6	45.000004	32
GATCGAC	30	2.1647356E-6	45.000004	9
CCCTAAT	30	2.1647356E-6	45.000004	33
GCGCGAT	30	2.1647356E-6	45.000004	1
GCTATCG	30	2.1647356E-6	45.000004	33
GCCGGTC	30	2.1647356E-6	45.000004	9
CAATTGT	30	2.1647356E-6	45.000004	36
CGCGATT	30	2.1647356E-6	45.000004	2
TACGGGA	30	2.1647356E-6	45.000004	4
ATGTCAC	30	2.1647356E-6	45.000004	45
GGCTTCG	30	2.1647356E-6	45.000004	1
CGGATGA	30	2.1647356E-6	45.000004	19
TAATTCA	30	2.1647356E-6	45.000004	14
CGCTAGG	60	0.0	45.000004	2
CGCTAAG	30	2.1647356E-6	45.000004	40
GTCGTAA	30	2.1647356E-6	45.000004	24
CAGGATG	30	2.1647356E-6	45.000004	3
CGTACTC	30	2.1647356E-6	45.000004	23
TACTCTA	30	2.1647356E-6	45.000004	41
>>END_MODULE