##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548158_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	322063
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.819047826046457	30.0	25.0	31.0	16.0	33.0
2	28.67950059460416	31.0	28.0	33.0	16.0	34.0
3	26.968472007029682	31.0	25.0	33.0	16.0	34.0
4	30.818501349114925	33.0	28.0	35.0	19.0	37.0
5	32.61930740258894	35.0	32.0	35.0	28.0	37.0
6	33.76634385197927	35.0	33.0	37.0	29.0	37.0
7	33.328137662507025	35.0	32.0	36.0	28.0	37.0
8	33.57786830526947	35.0	33.0	37.0	28.0	37.0
9	35.29906260576347	37.0	35.0	39.0	30.0	39.0
10	35.62888937878614	37.0	35.0	39.0	30.0	39.0
11	35.603285692550834	37.0	35.0	39.0	30.0	39.0
12	34.368108103073	35.0	33.0	39.0	27.0	39.0
13	34.146533442214725	35.0	33.0	39.0	25.0	39.0
14	34.86056454793006	37.0	33.0	40.0	25.0	41.0
15	35.786392724404855	37.0	34.0	40.0	30.0	41.0
16	35.471802100831205	36.0	34.0	40.0	30.0	41.0
17	35.52950199184632	36.0	34.0	40.0	30.0	41.0
18	35.22189136907996	36.0	34.0	39.0	29.0	40.0
19	34.681431893759914	36.0	33.0	39.0	27.0	40.0
20	35.037051756954384	35.0	34.0	39.0	29.0	40.0
21	35.07692283807826	35.0	34.0	39.0	29.0	40.0
22	35.235525347525176	35.0	34.0	39.0	30.0	41.0
23	35.45461602233104	35.0	34.0	40.0	30.0	41.0
24	35.3383344252522	35.0	34.0	40.0	30.0	41.0
25	34.58009147278638	35.0	33.0	39.0	27.0	41.0
26	34.574039861766174	35.0	33.0	39.0	27.0	41.0
27	34.84018654735254	35.0	34.0	39.0	28.0	41.0
28	34.79751166697199	35.0	34.0	40.0	27.0	41.0
29	34.91614373585292	36.0	34.0	40.0	29.0	41.0
30	34.53806242877946	36.0	33.0	40.0	27.0	41.0
31	34.38866619263933	35.0	33.0	39.0	26.0	41.0
32	34.068980292675654	35.0	33.0	39.0	24.0	41.0
33	33.978929588310365	35.0	33.0	40.0	23.0	41.0
34	33.87371104411249	35.0	33.0	40.0	23.0	41.0
35	33.705479983729894	35.0	33.0	40.0	21.0	41.0
36	33.446319508915956	35.0	33.0	40.0	19.0	41.0
37	33.326970189062386	35.0	33.0	40.0	18.0	41.0
38	33.24358898724784	35.0	33.0	40.0	18.0	41.0
39	33.13590198191037	35.0	33.0	40.0	18.0	41.0
40	33.001502811561714	35.0	32.0	40.0	18.0	41.0
41	32.969313457304935	35.0	32.0	40.0	18.0	41.0
42	32.98504019399931	35.0	32.0	39.0	18.0	41.0
43	32.946156497331266	35.0	32.0	39.0	18.0	41.0
44	32.798644985608405	35.0	32.0	39.0	18.0	41.0
45	32.67375948184051	35.0	32.0	39.0	18.0	40.0
46	32.59822146598647	35.0	32.0	39.0	18.0	40.0
47	32.63635686185622	35.0	32.0	39.0	18.0	40.0
48	32.538897669089586	35.0	32.0	39.0	17.0	40.0
49	32.54924347099791	35.0	32.0	39.0	17.0	40.0
50	32.35189077913328	35.0	32.0	39.0	15.0	40.0
51	30.84542775792314	34.0	29.0	37.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	18.0
9	52.0
10	62.0
11	56.0
12	60.0
13	56.0
14	65.0
15	109.0
16	226.0
17	351.0
18	683.0
19	1070.0
20	1649.0
21	2206.0
22	3081.0
23	4285.0
24	5730.0
25	7372.0
26	8383.0
27	8681.0
28	9082.0
29	9981.0
30	11880.0
31	15044.0
32	19941.0
33	27843.0
34	38913.0
35	32469.0
36	28255.0
37	33700.0
38	36119.0
39	14640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.423954319496495	3.4139283307924226	48.6476248435865	27.514492506124576
2	24.62033825680069	5.038144710817448	50.74038309274893	19.601133939632927
3	22.322651158313747	4.057591216625318	53.13929262287193	20.480465002189014
4	18.55196033074274	4.278976473547101	52.175195536277066	24.993867659433093
5	16.48217895256518	5.9631190170867185	55.380158540409795	22.1745434899383
6	19.36950224024492	7.090227688371529	57.92779673542133	15.612473335962218
7	80.8686499225307	1.9940198035788028	14.574788162564467	2.562542111326045
8	83.12410925812651	3.0195955449710152	11.91164461611548	1.944650580786989
9	78.87959809105672	4.582022771942135	12.798738135085372	3.7396410019157744
10	49.43225393789414	22.530374491947228	18.14334462511993	9.894026945038704
11	44.12552823515896	18.321881122637496	24.95039790351577	12.602192738687773
12	38.994234047375826	19.939577039274926	28.368673209899924	12.697515703449325
13	21.788283658787254	35.94079419244061	29.52372672427444	12.747195424497693
14	15.507214426990993	38.17576064310399	31.013807857468876	15.303217072436137
15	13.819035406116194	21.40109233286655	50.71864821479027	14.06122404622698
16	16.154603291902518	16.579364906866047	49.87999242384254	17.386039377388897
17	16.793608703887127	16.70946367636146	34.283354498964485	32.213573120786926
18	20.955527334714017	20.963910787640927	36.768582544409014	21.311979333236046
19	26.53859648578073	21.08034763384804	32.9621844173345	19.418871463036737
20	29.919612001378614	20.761776422625385	30.56793236105979	18.75067921493621
21	24.255502805351746	24.437144285434837	32.92150914572615	18.385843763487266
22	23.155407482386988	22.54279442220932	31.443847942793802	22.85795015260989
23	20.24728081151824	26.710923018167254	30.651456392072358	22.390339778242147
24	20.008818150486086	22.051275682087045	39.67174124317292	18.26816492425395
25	17.160617643131935	23.24979895237888	38.156509751197746	21.433073653291437
26	17.21961231187687	29.923958976970344	32.911573201516475	19.944855509636312
27	18.57183221916209	29.985127133511146	32.803830306492834	18.639210340833937
28	15.63855518951261	27.455808335636195	41.60614538149368	15.299491093357512
29	16.024194024150553	23.977917363994	39.62547700294663	20.372411608908816
30	17.919164883889177	26.245796629851924	37.64977659650441	18.18526188975449
31	26.572751294001485	24.9944886559462	29.84012444770123	18.592635602351095
32	26.91771485703108	25.248165731549417	30.193471463657733	17.640647947761774
33	25.726022548383394	25.31523335496471	29.4700105258909	19.488733570761
34	21.448909064375606	26.945349201864232	30.94736123056669	20.658380503193474
35	19.24716592716332	25.626663106286657	32.94293352542825	22.18323744112177
36	28.548762198700256	24.03722253099549	28.079288834793193	19.334726435511065
37	22.020225856431814	29.091202652897103	31.045478679637213	17.843092811033866
38	21.397366353787923	30.998282944641264	26.938518240220084	20.665832461350732
39	20.76798638775643	29.779266789416976	28.98935922474795	20.463387598078636
40	22.784983062320105	26.14333220518967	27.53498539105703	23.5366993414332
41	18.472162278808803	25.849911352747757	27.924350204773603	27.75357616366984
42	22.623834467169466	26.519966590387593	26.599143645808425	24.25705529663451
43	23.107590750877936	28.04761801262486	25.651502966810842	23.193288269686366
44	21.06016524717214	31.004182411515757	28.44878176009042	19.486870581221684
45	17.237621210756902	34.02067297392125	26.06073966894676	22.680966146375088
46	21.598258725777256	31.314370169811497	27.736188261302914	19.351182843108337
47	22.31768318620891	26.832327836479198	28.885652807059486	21.964336170252405
48	24.50949037921152	23.61711838988024	29.84602391457572	22.02736731633252
49	21.74916087846167	23.646615724252708	32.188733260262744	22.415490137022882
50	20.168414254353962	31.287046323234897	27.35862238133533	21.185917041075815
51	20.434821758475827	31.726401356256385	24.729944141363646	23.10883274390414
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	97.5
2	189.0
3	1966.5
4	3744.0
5	3350.0
6	2956.0
7	2974.0
8	2992.0
9	3110.0
10	3228.0
11	3171.0
12	3114.0
13	3034.5
14	2955.0
15	2823.5
16	2692.0
17	2499.0
18	2306.0
19	2198.0
20	2090.0
21	2061.0
22	2032.0
23	2175.0
24	2318.0
25	2250.0
26	2244.5
27	2307.0
28	2466.0
29	2625.0
30	3159.0
31	3693.0
32	4603.5
33	5514.0
34	6384.0
35	7254.0
36	8205.0
37	9156.0
38	10868.5
39	12581.0
40	16819.0
41	21057.0
42	24774.5
43	28492.0
44	28701.5
45	28911.0
46	28664.0
47	28417.0
48	26647.0
49	24877.0
50	23787.0
51	22697.0
52	20938.5
53	19180.0
54	18238.5
55	17297.0
56	15571.0
57	13845.0
58	12835.0
59	11825.0
60	10514.0
61	9203.0
62	8192.5
63	7182.0
64	6131.0
65	5080.0
66	4000.5
67	2921.0
68	2415.5
69	1910.0
70	1612.5
71	1315.0
72	1058.0
73	801.0
74	593.5
75	348.5
76	311.0
77	217.0
78	123.0
79	131.0
80	139.0
81	115.5
82	92.0
83	63.0
84	34.0
85	29.0
86	24.0
87	16.0
88	8.0
89	4.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	322063.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.870392735389895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.95874489431749	25.483166024107508
2	8.41779187427808	5.365566659960325
3	2.8577504645252736	2.7323288893249016
4	1.4704719555730106	1.874580749219546
5	0.9056584705756558	1.4431845570689354
6	0.629761761569015	1.2042452802561288
7	0.4768195817975963	1.0637499135069637
8	0.3728589163092023	0.9506528078132922
9	0.2848922013707024	0.8171663710440662
>10	3.309747644615133	26.03718252282651
>50	1.1735559452665834	25.64564354817075
>100	0.13794780276557794	6.309543851122642
>500	0.002998865277512564	0.647680012589363
>1k	9.996217591708547E-4	0.42530881298907997
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1335	0.4145151724973064	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGC	825	0.2561610616556388	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	658	0.20430785281140645	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	550	0.1707740411037592	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	497	0.15431763350648786	No Hit
TTCTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	443	0.13755072765266424	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG	435	0.1350667416002459	No Hit
TTTTCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	386	0.11985232702918372	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010556940722777842	0.0
2	0.0	0.0	0.0	0.14251869975750087	0.0
3	0.0	0.0	0.0	0.1530756404802787	0.0
4	0.0	0.0	0.0	0.24312013488044265	0.0
5	0.0	0.0	0.0	0.528778530908549	0.0
6	0.0	3.104982565522895E-4	0.0	0.5765952624176015	0.0
7	0.0	3.104982565522895E-4	0.0	0.6430418893197914	0.0
8	0.0	3.104982565522895E-4	0.0	0.7340178784896123	0.0
9	0.0	3.104982565522895E-4	0.0	0.7604102302965569	0.0
10	0.0	3.104982565522895E-4	0.0	0.9895579436321466	0.0
11	0.0	3.104982565522895E-4	0.0	1.069355995566085	0.0
12	0.0	3.104982565522895E-4	0.0	1.5298249100331303	0.0
13	0.0	3.104982565522895E-4	0.0	1.6198694044332942	0.0
14	0.0	3.104982565522895E-4	0.0	1.6558872021933597	0.0
15	0.0	3.104982565522895E-4	0.0	1.6816585574871996	0.0
16	0.0	3.104982565522895E-4	0.0	1.7263703064307294	0.0
17	0.0	3.104982565522895E-4	0.0	1.7856754734322167	0.0
18	0.0	3.104982565522895E-4	0.0	1.8772724591151422	0.0
19	0.0	3.104982565522895E-4	0.0	1.9567600127925282	0.0
20	0.0	3.104982565522895E-4	0.0	2.0008507652229532	0.0
21	0.0	3.104982565522895E-4	0.0	2.0430785281140644	0.0
22	0.0	3.104982565522895E-4	0.0	2.0744388520258457	0.0
23	0.0	3.104982565522895E-4	0.0	2.113251134094882	0.0
24	0.0	3.104982565522895E-4	0.0	2.1405749806714836	0.0
25	0.0	3.104982565522895E-4	0.0	2.1703828133005034	0.0
26	0.0	3.104982565522895E-4	0.0	2.194601677311582	0.0
27	0.0	3.104982565522895E-4	0.0	2.221615025631631	0.0
28	0.0	3.104982565522895E-4	0.0	2.248317875695128	0.0
29	0.0	3.104982565522895E-4	0.0	2.279367701350357	0.0
30	0.0	3.104982565522895E-4	0.0	2.31507500085387	0.0
31	0.0	3.104982565522895E-4	0.0	2.3551292759491154	0.0
32	0.0	3.104982565522895E-4	0.0	2.3827636207822693	0.0
33	0.0	3.104982565522895E-4	0.0	2.410397965615423	0.0
34	0.0	3.104982565522895E-4	0.0	2.4448632720927272	0.0
35	0.0	3.104982565522895E-4	0.0	2.4861595402141816	0.0
36	0.0	3.104982565522895E-4	0.0	2.527455808335636	0.0
37	0.0	3.104982565522895E-4	0.0	2.5640946026088063	0.0
38	0.0	3.104982565522895E-4	0.0	2.5976284143164534	0.0
39	0.0	3.104982565522895E-4	0.0	2.6361301981289373	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTA	25	3.8845315E-5	45.000004	25
CGAACAA	25	3.8845315E-5	45.000004	36
CGTATTA	25	3.8845315E-5	45.000004	34
TTAGCGA	25	3.8845315E-5	45.000004	22
GGTCATA	25	3.8845315E-5	45.000004	8
GTGATAA	25	3.8845315E-5	45.000004	28
ATAGACG	25	3.8845315E-5	45.000004	14
CGTTCGG	25	3.8845315E-5	45.000004	2
TATTGCG	25	3.8845315E-5	45.000004	1
CCGTATT	25	3.8845315E-5	45.000004	33
CCCGTAT	25	3.8845315E-5	45.000004	32
CCCCGTA	25	3.8845315E-5	45.000004	31
TAACCGT	25	3.8845315E-5	45.000004	39
CTACGAA	35	1.2082819E-7	45.000004	11
TACGAAT	35	1.2082819E-7	45.000004	12
CGAATAC	25	3.8845315E-5	45.000004	14
TAGGGCG	35	1.2082819E-7	45.000004	5
CCGACCT	25	3.8845315E-5	45.000004	21
ATGCGAA	25	3.8845315E-5	45.000004	33
CGCGTCG	25	3.8845315E-5	45.000004	15
>>END_MODULE