FastQCFastQC Report
Sat 18 Jun 2016
SRR3548157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929931
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC86070.9255525409949771No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG66540.7155369591937466No Hit
GAATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC50700.5452017407743155No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49200.5290715117573239No Hit
GAACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT14450.15538787286368558No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA13570.1459248051737172No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13260.1425912245102056No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTC13200.14194601534952592No Hit
TTTTTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC12380.1331281568202372No Hit
GATTGATACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC11790.12678360007355385No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCT11100.11936369472573773No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTA11010.11839588098471823No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC10740.11549243976165972TruSeq Adapter, Index 20 (95% over 21bp)
GATTGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG10110.10871774357452328No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAAGGTCCT9400.10108276850648058No Hit
GATTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC9390.10097523364636732No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTTA750.045.00000437
CGGAACA207.0325914E-445.029
AATCCCG207.0325914E-445.027
TCGTTCC207.0325914E-445.030
TCGTTAC207.0325914E-445.043
ACACGTA207.0325914E-445.08
TCACCGT207.0325914E-445.024
CTCAATC253.8902286E-545.027
CGTATCA207.0325914E-445.038
TACTTGC502.1827873E-1145.014
TACCATA207.0325914E-445.031
CACGGTA253.8902286E-545.034
GTCGAAG351.2115925E-745.016
CAAGTCG406.8121153E-945.015
TACCACC351.2115925E-745.042
TTTACGT253.8902286E-545.025
CACGGCA207.0325914E-445.038
ACGTTGC351.2115925E-745.012
TCACATG453.8380676E-1045.036
CCGATAG253.8902286E-545.014