FastQCFastQC Report
Sat 18 Jun 2016
SRR3548156_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548156_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225150
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGC11800.5240950477459472No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC8720.387297357317344No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCG7280.32333999555851656No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.3220075505218743No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA5270.23406617810348657No Hit
TTTTTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC3450.15323117921385743No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3450.15323117921385743No Hit
TTTTTTGGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAG3030.1345769487008661No Hit
TTCTTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC2980.13235620697312903No Hit
GTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA2870.12747057517210747No Hit
TTGCTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC2670.11858760826115923No Hit
TTTCTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA2320.10304241616699977No Hit
TTGCTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA2320.10304241616699977No Hit
TTGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA2310.10259826782145237No Hit
TTTTTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA2300.10215411947590496No Hit
TTCTTTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA2300.10215411947590496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTCG406.7739165E-945.0000041
CGGGTGC207.021192E-445.0000046
CGGGTAC207.021192E-445.0000046
AGCCGTG207.021192E-445.00000444
TAGAGGT207.021192E-445.00000439
CAGCGGT207.021192E-445.00000427
ACTATAT207.021192E-445.00000418
CTGTCCA207.021192E-445.00000426
GCCCAAT207.021192E-445.00000418
ATAGGTG207.021192E-445.00000445
GTTTCGG406.7739165E-945.0000042
CGTGCAC207.021192E-445.00000418
TAGACCA207.021192E-445.00000430
ACGCACA207.021192E-445.00000416
TCCTGGT207.021192E-445.00000436
GGTCCAC207.021192E-445.0000048
ATGCTAG207.021192E-445.00000441
TTCGTCC207.021192E-445.00000410
CTCCACA207.021192E-445.00000418
GTCTAAT207.021192E-445.00000418