##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548155_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	744369
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.69634146505295	30.0	25.0	31.0	16.0	33.0
2	28.470743676859193	31.0	28.0	33.0	16.0	34.0
3	26.33383711573158	30.0	19.0	33.0	10.0	34.0
4	30.310359512553585	33.0	28.0	35.0	19.0	37.0
5	32.446421062671874	35.0	32.0	35.0	28.0	37.0
6	33.67480107312368	35.0	33.0	37.0	29.0	37.0
7	33.48003073744339	35.0	32.0	35.0	29.0	37.0
8	33.577682574099676	35.0	33.0	37.0	28.0	37.0
9	35.345606815974335	37.0	35.0	39.0	30.0	39.0
10	35.65512803461724	37.0	35.0	39.0	30.0	39.0
11	35.630941105822515	37.0	35.0	39.0	30.0	39.0
12	34.42928305719341	35.0	33.0	39.0	27.0	39.0
13	34.17825836379538	37.0	33.0	39.0	25.0	39.0
14	35.02693825239901	37.0	33.0	40.0	25.0	41.0
15	35.90325497166056	38.0	34.0	40.0	30.0	41.0
16	35.692097602130126	37.0	34.0	40.0	30.0	41.0
17	35.61471796917927	37.0	34.0	40.0	30.0	41.0
18	35.312711840498466	37.0	34.0	39.0	29.0	40.0
19	34.58508212996511	36.0	33.0	39.0	27.0	40.0
20	34.77414964889726	35.0	34.0	39.0	29.0	40.0
21	34.921576529919975	35.0	34.0	39.0	29.0	40.0
22	35.06720725876548	35.0	33.0	39.0	29.0	40.0
23	35.329814917063985	35.0	34.0	39.0	30.0	41.0
24	35.19411071659352	35.0	34.0	40.0	29.0	41.0
25	34.4954411051508	35.0	33.0	39.0	27.0	40.0
26	34.41818641023471	35.0	33.0	39.0	25.0	40.0
27	34.72043166762721	35.0	33.0	39.0	27.0	41.0
28	34.61612049937598	35.0	33.0	39.0	27.0	41.0
29	34.6542588420528	35.0	34.0	40.0	27.0	41.0
30	34.261418731838646	35.0	33.0	39.0	24.0	41.0
31	34.05841860690061	35.0	33.0	39.0	24.0	41.0
32	33.573264066612126	35.0	33.0	39.0	22.0	41.0
33	33.327074340817525	35.0	33.0	40.0	19.0	41.0
34	33.107317741603964	35.0	33.0	40.0	18.0	41.0
35	32.858036807013725	35.0	33.0	40.0	15.0	41.0
36	32.58698172546143	35.0	32.0	40.0	13.0	41.0
37	32.450501028387805	35.0	32.0	40.0	12.0	41.0
38	32.37239191852428	35.0	31.0	40.0	12.0	41.0
39	32.18744869815911	35.0	31.0	40.0	10.0	41.0
40	32.086623704103744	35.0	31.0	39.0	10.0	41.0
41	31.991195227098387	35.0	31.0	39.0	10.0	41.0
42	31.995909286926242	35.0	31.0	39.0	10.0	41.0
43	31.95417595305554	35.0	31.0	39.0	10.0	41.0
44	31.79052862222903	35.0	31.0	39.0	10.0	41.0
45	31.749061285464602	35.0	30.0	39.0	10.0	40.0
46	31.565998852719552	35.0	30.0	39.0	10.0	40.0
47	31.630179386836367	35.0	30.0	39.0	10.0	40.0
48	31.525810451536806	35.0	30.0	39.0	10.0	40.0
49	31.57531949879697	35.0	30.0	39.0	10.0	40.0
50	31.42614079844808	35.0	30.0	39.0	10.0	40.0
51	29.94343128206575	34.0	26.0	37.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	33.0
9	86.0
10	106.0
11	103.0
12	102.0
13	114.0
14	153.0
15	275.0
16	510.0
17	993.0
18	1844.0
19	2978.0
20	4602.0
21	6495.0
22	9109.0
23	12500.0
24	16869.0
25	21776.0
26	24375.0
27	24422.0
28	23213.0
29	24307.0
30	28026.0
31	34608.0
32	45093.0
33	61130.0
34	82938.0
35	70662.0
36	62495.0
37	73110.0
38	77799.0
39	33539.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.724357140074346	3.5037729943079308	47.2674171009271	29.50445276469063
2	27.480187917551646	7.176951216399393	49.220480702447304	16.122380163601655
3	18.24175912752949	6.90907332250537	54.87359092063211	19.975576629333034
4	16.80013541670865	3.5204313989432663	59.07298665043815	20.606446533909928
5	17.34892237586466	4.7837833117714466	57.87143204512816	19.99586226723574
6	17.914367739656004	8.857703638921018	59.60766770244328	13.6202609189797
7	74.86125832752305	1.7735827257717611	21.237047754541095	2.1281111921641016
8	77.23467796213974	4.6945802417886835	16.466161272164744	1.6045805239068258
9	73.79485174691584	3.185785544535036	16.779178068941615	6.240184639607507
10	47.256266717179244	21.97418215965469	21.61199620080901	9.157554922357058
11	40.18746078893667	16.743577446132228	30.948091605104455	12.120870159826644
12	35.52364485893421	17.493339996695187	32.689029231469874	14.293985912900725
13	18.86738969516463	32.3014526397526	36.211878785924725	12.619278879158053
14	14.712998526268558	34.68320147668697	36.327412882589144	14.27638711445533
15	12.59147009077487	20.239961631932548	54.03314753838486	13.135420738907719
16	14.162465121465296	16.54098975105089	51.659996587713884	17.636548539769926
17	15.627061309646157	16.063001011595055	38.68847305570221	29.621464623056575
18	19.09160644787733	18.945845407318146	42.41579109285851	19.54675705194601
19	22.868631014993905	21.127827730601357	35.20283622773114	20.8007050266736
20	26.589634979425526	19.908136958954497	36.36072969185982	17.141498369760157
21	20.523154510733253	24.01510541142901	37.42270298736245	18.039037090475286
22	20.922015828171244	19.82914387890952	37.3787731622354	21.87006713068384
23	18.72391246814416	24.51861912572931	35.0803163484777	21.677152057648826
24	18.812712512208325	19.78131813656936	42.94456109805755	18.46140825316476
25	16.183237077309776	22.2561659606996	42.18552895136686	19.375068010623764
26	15.885535265439588	28.07505417340056	35.99814070709554	20.041269854064314
27	16.206881264534122	30.142308451856536	36.32042710000014	17.330383183609204
28	14.11302727545075	25.7506693588798	44.252917571795706	15.883385793873735
29	15.018357830592086	21.661568388796418	42.457974472338314	20.862099308273184
30	14.770631232627904	26.76293612442216	39.23591659512957	19.230516047820366
31	24.203721541332325	24.723356292376497	31.64290828876538	19.4300138775258
32	24.85783260721497	24.854877083811928	33.74925608132526	16.538034227647845
33	22.42275000705295	25.62573132411479	31.600053199421257	20.351465469411004
34	19.2475774783743	25.86002372479241	32.934472015895345	21.957926780937946
35	18.42379250076239	25.609341603425186	33.01870443288208	22.948161462930347
36	25.19006030611162	22.9757015673678	30.856873405528713	20.977364720991872
37	19.602240286739505	29.248799990327377	34.08994732451244	17.059012398420677
38	18.391550427274645	28.04294644188568	29.49263067107846	24.07287245976122
39	19.870252522606396	27.578794925635002	31.988570184948596	20.56238236681001
40	20.277040016443458	24.942333708147437	31.100838428252654	23.67978784715645
41	16.503911366539985	25.58032373728621	31.0354138874671	26.880351008706704
42	19.529561279419212	25.86365095805978	30.34731430244946	24.25947346007155
43	22.11967451626814	25.516511300175047	30.18946248433237	22.17435169922444
44	19.588403063534347	29.11109946814013	30.59571261027797	20.704784858047553
45	16.904787813570955	33.23687579681583	26.608577197599576	23.249759192013638
46	22.345637714628094	29.96390231189101	29.295416654911744	18.39504331856915
47	19.763853680096837	27.80059352283612	32.60775233788618	19.827800459180864
48	20.96285578792239	25.647494723719017	30.63547783424619	22.754171654112408
49	19.617017903754725	23.01694455303754	34.5475160840927	22.818521459115036
50	19.930437726450187	28.508307035892145	30.00057767048332	21.560677567174345
51	17.67429863414516	29.30025296593491	28.363352047170153	24.662096352749778
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	29.0
1	283.5
2	538.0
3	7179.0
4	13820.0
5	12701.0
6	11582.0
7	11491.0
8	11400.0
9	11728.5
10	12057.0
11	11741.5
12	11426.0
13	10772.5
14	10119.0
15	9182.0
16	8245.0
17	7628.0
18	7011.0
19	6512.5
20	6014.0
21	5441.5
22	4869.0
23	4985.0
24	5101.0
25	5441.5
26	6529.5
27	7277.0
28	8803.0
29	10329.0
30	11893.0
31	13457.0
32	14715.5
33	15974.0
34	17035.0
35	18096.0
36	20014.5
37	21933.0
38	25823.0
39	29713.0
40	35721.5
41	41730.0
42	49776.0
43	57822.0
44	59015.0
45	60208.0
46	56692.0
47	53176.0
48	64841.5
49	76507.0
50	63928.0
51	51349.0
52	45537.5
53	39726.0
54	33949.0
55	28172.0
56	27303.5
57	26435.0
58	27355.0
59	28275.0
60	24046.5
61	19818.0
62	16730.0
63	13642.0
64	11595.0
65	9548.0
66	7613.0
67	5678.0
68	4463.5
69	3249.0
70	2560.0
71	1871.0
72	1477.0
73	1083.0
74	875.5
75	510.0
76	352.0
77	245.5
78	139.0
79	104.0
80	69.0
81	48.5
82	28.0
83	28.5
84	29.0
85	19.0
86	9.0
87	9.0
88	9.0
89	5.0
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	744369.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.653619187733963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.67555112116065	23.97607377149503
2	7.196034827291944	4.123848832057886
3	2.511565323582887	2.158963090411856
4	1.209554004256559	1.3863239929986477
5	0.7544134840547017	1.0808338341097516
6	0.5445906903962633	0.9362696553479793
7	0.39120587185573363	0.7846624853311751
8	0.2951284167793639	0.6765197812699782
9	0.23118041541460313	0.5961740028256963
>10	1.6883825624556643	10.603379524593818
>50	0.7545480604259583	16.09206926329434
>100	0.7363546427252765	32.835635418611595
>500	0.00813916055027939	1.6926023697199084
>1k	0.002872644900098608	2.198681972726615
>5k	4.7877415001643467E-4	0.857962005205731
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGC	6254	0.8401746983015145	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4465	0.5998369088449412	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCG	4410	0.5924481003373328	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC	3246	0.4360740439217646	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1359	0.1825707411243617	No Hit
TTTTTTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA	1355	0.18203337323289928	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1192	0.16013563165580513	No Hit
GAACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCT	911	0.12238553728056917	No Hit
TTCCTTGGGGTCAGAAACAAATTCTCAACAATGTATTTTTCTAATGAACCC	893	0.11996738176898822	No Hit
GATTGATACGGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGC	887	0.11916132993179458	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTC	812	0.10908568196687396	No Hit
TGTTTTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA	795	0.1068018684281586	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTA	792	0.10639884250956179	No Hit
TTCTTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC	746	0.10021911175774381	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03640667464657985	0.0
2	0.0	0.0	0.0	0.23953173761937963	0.0
3	0.0	0.0	0.0	0.27163946913426	0.0
4	0.0	0.0	0.0	0.5188286992069794	0.0
5	0.0	0.0	0.0	1.3612872110472092	0.0
6	0.0	0.0	0.0	1.4205320210809425	0.0
7	0.0	0.0	0.0	1.6149248558174776	0.0
8	0.0	0.0	0.0	1.8482768626850392	0.0
9	0.0	0.0	0.0	1.9009389160483576	0.0
10	0.0	0.0	0.0	2.83448128549147	0.0
11	0.0	0.0	0.0	2.9338943454120203	0.0
12	0.0	0.0	0.0	4.248161865956266	0.0
13	0.0	0.0	0.0	4.376995817934385	0.0
14	0.0	0.0	0.0	4.451555612874797	0.0
15	0.0	0.0	0.0	4.642858582235424	0.0
16	0.0	0.0	0.0	4.761482544275756	0.0
17	0.0	0.0	0.0	4.85283508582437	0.0
18	0.0	0.0	0.0	4.9581591925510065	0.0
19	0.0	0.0	0.0	5.134684544896416	0.0
20	0.0	0.0	0.0	5.215155386642915	0.0
21	0.0	0.0	0.0	5.287162684098881	0.0
22	0.0	0.0	0.0	5.365349712306665	0.0
23	0.0	0.0	0.0	5.431848988875141	0.0
24	0.0	0.0	0.0	5.490825114963143	0.0
25	0.0	0.0	0.0	5.537307437574643	0.0
26	0.0	0.0	0.0	5.588760413182172	0.0
27	0.0	0.0	0.0	5.641959834436952	0.0
28	0.0	0.0	0.0	5.687904789156991	0.0
29	0.0	0.0	0.0	5.745268811570606	0.0
30	0.0	0.0	0.0	5.8084095388174415	0.0
31	0.0	0.0	0.0	5.8741027635487235	0.0
32	0.0	0.0	0.0	5.932407179772397	0.0
33	0.0	0.0	0.0	5.985069233135716	0.0
34	0.0	0.0	0.0	6.049956406029805	0.0
35	0.0	0.0	0.0	6.117799102326937	0.0
36	0.0	0.0	0.0	6.186447850461263	0.0
37	0.0	0.0	0.0	6.247976474033711	0.0
38	0.0	0.0	0.0	6.316625222168038	0.0
39	0.0	0.0	0.0	6.385542654248095	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGAAC	30	2.1644664E-6	45.000004	13
TCGTTAC	35	1.2111559E-7	45.000004	23
GTACCAT	35	1.2111559E-7	45.000004	10
CGAACCC	35	1.2111559E-7	45.000004	32
GCGAACC	30	2.1644664E-6	45.000004	32
GACGTCA	30	2.1644664E-6	45.000004	38
CTCCCGC	30	2.1644664E-6	45.000004	38
TACGGGA	30	2.1644664E-6	45.000004	4
TCGATGT	30	2.1644664E-6	45.000004	16
TCTAGCG	60	0.0	45.000004	1
ACGGGAT	30	2.1644664E-6	45.000004	5
CGTTACA	35	1.2111559E-7	45.000004	24
ATTCCGA	30	2.1644664E-6	45.000004	12
AACGGAT	35	1.2111559E-7	45.000004	35
CCACTAA	35	1.2111559E-7	45.000004	34
TCTACGA	35	1.2111559E-7	45.000004	28
TGATTAC	70	0.0	45.000004	19
CACTAAG	35	1.2111559E-7	45.000004	35
AACGCTA	30	2.1644664E-6	45.000004	10
TCGCTAT	30	2.1644664E-6	45.000004	23
>>END_MODULE