FastQCFastQC Report
Mon 15 Aug 2016
SRR2921712_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921712_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3436286
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.046.2359731
GTATCAA44550.043.0413171
TCAACGC52850.035.5643424
CAACGCA55050.034.054365
ATCAACG56550.033.321343
TATCAAC56800.032.924472
AACGCAG61300.030.95186
GTACATG92200.028.518771
TACATGG95950.027.0096262
ACATGGG100300.025.5539153
ACGCAGA71450.025.4299877
CGTATAC2301.364242E-1024.763843
CGCAGAG74500.024.0722528
CATGGGG85850.023.1100314
GCAGAGT85050.019.5251279
ATGGGGG51100.018.6684465
AGAGTAC70250.018.23589510-11
GAGTACA68400.017.5556281
TAAGGTA9300.017.352444
TAATACA11800.017.2962134
Produced by FastQC (version 0.11.5)