##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921712_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3436286
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.290284045041652	31.0	31.0	34.0	26.0	34.0
2	30.346461266611684	31.0	31.0	34.0	26.0	34.0
3	30.40775011160305	33.0	31.0	34.0	26.0	34.0
4	33.85463666295529	37.0	35.0	37.0	30.0	37.0
5	33.84789711915714	37.0	35.0	37.0	30.0	37.0
6	33.89131929065276	37.0	35.0	37.0	30.0	37.0
7	33.80012635735209	37.0	35.0	37.0	29.0	37.0
8	33.7672562761074	37.0	35.0	37.0	29.0	37.0
9	35.31569694722732	39.0	37.0	39.0	29.0	39.0
10-11	35.21171855311229	39.0	35.0	39.0	29.0	39.0
12-13	35.09855888013978	39.0	35.0	39.0	28.0	39.0
14-15	36.23154417298211	40.0	36.5	41.0	27.0	41.0
16-17	36.164939559745605	40.0	36.0	41.0	27.0	41.0
18-19	36.12738637005185	40.0	36.5	41.0	27.0	41.0
20-21	36.04678801473452	40.0	36.0	41.0	27.0	41.0
22-23	35.94600594944659	40.0	36.0	41.0	27.0	41.0
24-25	35.831902379487616	40.0	36.0	41.0	27.0	41.0
26-27	35.68212482895778	40.0	36.0	41.0	26.5	41.0
28-29	35.545166496618734	40.0	36.0	41.0	25.5	41.0
30-31	35.39675350072724	40.0	35.5	41.0	25.0	41.0
32-33	35.22046156809998	39.0	35.0	41.0	24.5	41.0
34-35	35.04219584749349	39.0	35.0	41.0	24.0	41.0
36-37	35.010908434280495	39.0	35.0	41.0	24.0	41.0
38-39	35.1909525283984	39.5	35.0	41.0	24.0	41.0
40-41	35.25046299987836	40.0	35.0	41.0	24.0	41.0
42-43	35.089428528358816	40.0	35.0	41.0	23.5	41.0
44-45	34.96854307237523	40.0	35.0	41.0	23.0	41.0
46-47	34.803663170062094	39.5	35.0	41.0	22.0	41.0
48-49	34.63836261009706	39.0	35.0	41.0	20.5	41.0
50-51	32.94983624762316	37.0	32.5	39.5	17.0	40.0
52-53	33.063453827766374	37.5	32.5	39.5	16.0	40.0
54-55	33.96073144086377	38.5	34.0	40.5	18.0	41.0
56-57	33.97365411377284	38.5	34.0	41.0	17.5	41.0
58-59	33.77506106884002	38.0	34.0	40.5	14.5	41.0
60-61	33.51431210906193	37.5	34.0	40.0	13.0	41.0
62-63	33.21422998551343	37.0	33.0	40.0	10.0	41.0
64-65	32.92033666580721	36.5	33.0	40.0	8.0	41.0
66-67	32.57786429883892	36.0	33.0	39.0	7.0	41.0
68-69	32.217329552895194	35.5	32.5	39.0	2.0	41.0
70-71	31.811185681284968	35.0	32.0	38.5	2.0	40.5
72-73	31.455269148144247	35.0	32.0	37.0	2.0	40.0
74-75	31.063913917526072	35.0	32.0	37.0	2.0	39.0
76-77	30.698277151552578	35.0	31.0	36.5	2.0	39.0
78-79	30.365584238331735	35.0	31.0	36.0	2.0	38.0
80-81	30.05796752656793	35.0	31.0	35.5	2.0	37.0
82-83	29.7579441874163	35.0	31.0	35.0	2.0	37.0
84-85	29.508882991695103	35.0	31.0	35.0	2.0	36.0
86-87	29.28231381206337	35.0	30.5	35.0	2.0	36.0
88-89	29.07227949012393	34.5	30.0	35.0	2.0	36.0
90-91	28.85251038475843	34.0	30.0	35.0	2.0	35.0
92-93	28.66785389807484	34.0	30.0	35.0	2.0	35.0
94-95	28.442893577542733	34.0	30.0	35.0	2.0	35.0
96-97	28.212188100757622	34.0	29.0	35.0	2.0	35.0
98-99	27.945021456304858	34.0	29.0	35.0	2.0	35.0
100-101	26.8783443811138	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	125512.0
3	21555.0
4	14524.0
5	11075.0
6	10097.0
7	10449.0
8	10962.0
9	11821.0
10	12722.0
11	13392.0
12	13433.0
13	13502.0
14	14205.0
15	14802.0
16	14668.0
17	15376.0
18	14863.0
19	14277.0
20	14389.0
21	15266.0
22	16686.0
23	19138.0
24	22310.0
25	26394.0
26	31273.0
27	37221.0
28	43882.0
29	51986.0
30	62235.0
31	75884.0
32	94147.0
33	121926.0
34	169443.0
35	246687.0
36	413025.0
37	739548.0
38	753754.0
39	123847.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.365059376232914	17.637412631698478	12.729996462320806	25.267531529747806
2	16.225093046147396	19.469768010763648	38.64024486134813	25.664894081740826
3	19.300146191166792	23.429580705095724	28.948151642757715	28.322121460979773
4	13.571328517982142	15.358287231437101	36.0798385732287	34.99054567735206
5	14.700877101023869	36.781400333135316	33.96783380508089	14.549888760759922
6	34.13548194256114	34.58628125348344	16.95720215128684	14.321034652668576
7	29.44668069538188	31.267648676731252	20.89530561934449	18.390365008542382
8	27.247960448015984	33.90244903412162	19.91232596972769	18.937264548134706
9	27.272023508272685	15.300778477253207	18.92725025443334	38.49994776004077
10-11	26.02541055641019	24.769216866076004	27.612744482414932	21.592628095098874
12-13	26.588321452798358	22.21894114183247	27.291475253465887	23.901262151903286
14-15	23.74623500771776	23.690193174017317	24.519510418540793	28.04406139972413
16-17	24.14595456297945	26.628467002321877	25.31546451831033	23.910113916388347
18-19	24.154516330648242	26.180362185394273	26.361403950438707	23.303717533518775
20-21	24.67336467674109	25.40255370426833	26.253118998951418	23.670962620039163
22-23	24.523178174616405	25.22246299464383	26.00470246152158	24.249656369218194
24-25	24.327522985989493	25.363901167231806	25.989771584007155	24.318804262771547
26-27	24.10467393417791	25.534002568428356	25.989517296141184	24.371806201252546
28-29	24.251580223673173	25.802175612487655	25.773493221293016	24.17275094254616
30-31	24.510504566402414	25.52645070353259	25.658802812238562	24.304241917826438
32-33	24.271626101204593	25.58669794325395	25.574227083157496	24.567448872383956
34-35	24.620047300464172	25.600019324129832	25.634724180787128	24.145209194618868
36-37	24.6690332904136	25.42721235110445	25.722111499437105	24.181642859044842
38-39	24.434343379960456	25.56413602794863	25.673138471518293	24.328382120572623
40-41	24.636008451678514	25.400317585454218	25.724698256713985	24.23897570615328
42-43	24.48841789638635	25.381137144036348	25.61510533527104	24.515339624306264
44-45	24.365419401342386	25.426174945279755	25.751777341788042	24.456628311589814
46-47	24.51469818695363	25.445417388209517	25.563503279912553	24.476381144924297
48-49	24.318219034005534	25.465560012779175	25.63855427308509	24.5776666801302
50-51	24.33288562578842	25.57482207853331	25.712487774551356	24.379804521126918
52-53	24.580844134019646	25.373553049235444	25.65689563436325	24.388707182381665
54-55	24.105578560597223	25.29601656363	25.92590108480112	24.672503790971657
56-57	24.156864300412003	25.461009639835947	25.813506968584214	24.56861909116784
58-59	24.324676271581893	25.277166938870742	25.79189512365842	24.60626166588894
60-61	24.246912730468804	25.335523319546997	26.02518523033865	24.39237871964555
62-63	24.31232978658566	25.37712687484453	25.907010219147537	24.40353311942227
64-65	24.15073111924146	25.22345343973946	25.86494342873373	24.760872012285347
66-67	24.15345779569434	25.29206633735071	25.822345806835035	24.73213006011992
68-69	24.227606312933673	25.24485304151975	25.85450708053061	24.673033565015963
70-71	24.16607319752427	25.082919241302243	25.830333185846836	24.92067437532665
72-73	24.16763261146274	25.283069211220415	25.715870801056194	24.83342737626065
74-75	24.15646534334869	25.336213192384704	25.804073647525488	24.70324781674112
76-77	24.252627895788077	25.052878657549826	25.86499686547798	24.82949658118412
78-79	24.159695296311796	25.106207375240785	25.894549426128748	24.83954790231867
80-81	24.334569375600566	25.21182056382136	25.760973915944945	24.69263614463313
82-83	24.246406375598937	25.217393713767716	25.858651940224675	24.67754797040867
84-85	24.248696064796686	25.258989741321596	25.779120150153606	24.71319404372811
86-87	24.224164788081602	25.328402339246793	25.746985512725374	24.70044735994623
88-89	24.162019055969527	25.137014640611753	25.825110413112682	24.875855890306035
90-91	24.15867617969983	25.11716998639787	25.903456656804174	24.82069717709813
92-93	24.319165345308978	24.909678055428184	25.992462135664056	24.778694463598782
94-95	24.23618891823074	24.866102070170946	26.012019237080064	24.885689774518248
96-97	24.207153114217764	24.73184026301453	26.10118737232703	24.959819250440678
98-99	24.09425841151763	24.700295769916995	26.029065348685155	25.17638046988023
100-101	24.36960007794762	24.61948754690495	26.034578171223966	24.97633420392346
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	48.0
1	39.0
2	71.5
3	109.5
4	92.5
5	99.0
6	164.5
7	318.0
8	508.5
9	587.5
10	529.5
11	502.0
12	549.0
13	670.0
14	794.0
15	848.5
16	959.0
17	1060.0
18	1155.5
19	1332.0
20	1439.0
21	1559.0
22	1716.5
23	1865.5
24	2099.0
25	2415.0
26	2908.5
27	3662.5
28	4866.0
29	6778.0
30	9752.5
31	14397.0
32	21075.5
33	27139.0
34	32845.5
35	40374.0
36	49621.0
37	59174.5
38	68470.5
39	77998.0
40	86923.0
41	95361.5
42	104223.0
43	112845.0
44	120851.5
45	130561.0
46	138458.5
47	144173.0
48	150214.5
49	153646.0
50	153765.5
51	154081.5
52	154139.0
53	153466.0
54	151171.0
55	144595.5
56	134944.0
57	122286.5
58	111053.5
59	98201.0
60	81934.0
61	68757.0
62	57037.5
63	43880.5
64	31864.5
65	23425.5
66	17722.0
67	13742.5
68	10480.5
69	7972.5
70	6110.0
71	4577.0
72	3332.5
73	2538.5
74	1975.0
75	1486.0
76	1113.0
77	735.5
78	490.0
79	324.5
80	237.5
81	183.0
82	143.0
83	102.0
84	81.0
85	69.0
86	66.0
87	60.0
88	53.0
89	43.0
90	35.5
91	35.5
92	31.5
93	25.5
94	21.5
95	19.0
96	15.0
97	12.0
98	8.0
99	8.0
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05328427261293152
2	0.07292757354888388
3	0.07059947862314138
4	0.07004655607827755
5	0.06536126504022075
6	0.011320361576422917
7	0.013852164808167889
8	0.01760621787592767
9	0.006373159859220099
10-11	0.01252806081915184
12-13	0.022247857134126785
14-15	0.039344804245048284
16-17	0.02664213630646576
18-19	0.009850751654547962
20-21	0.020036166954671412
22-23	0.0371476646588788
24-25	0.03346636455754847
26-27	0.021011056704826083
28-29	0.011058450897276886
30-31	0.01092749555770387
32-33	0.008031927493811633
34-35	0.004874448750773364
36-37	7.711814441522039E-4
38-39	0.003623097728186769
40-41	0.0013241039890160482
42-43	0.22781863907718972
44-45	0.19778621453511147
46-47	0.2037810589688984
48-49	0.21158017697013579
50-51	0.2026606632858848
52-53	0.2101542188281185
54-55	0.20621100804764214
56-57	0.20900472195853315
58-59	0.21785148267635465
60-61	0.22260952668084086
62-63	0.2076369661896594
64-65	0.20836449585395395
66-67	0.20535252304377458
68-69	0.20027436598699877
70-71	0.19187867366103986
72-73	0.20373740718904074
74-75	0.20229689845373758
76-77	0.1960255927475187
78-79	0.21479585808631763
80-81	0.2251122287260141
82-83	0.22448655321472077
84-85	0.21914648547879892
86-87	0.23787309903774015
88-89	0.23023403756264757
90-91	0.23708736700030208
92-93	0.23263488545481956
94-95	0.23615612903000507
96-97	0.24266024422879817
98-99	0.23614157843671918
100-101	0.24279119956837122
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3436286.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.22466110025276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.84733511125351	45.32634704410543
2	11.990185047125387	13.962489217963878
3	4.075170299990074	7.118262289282121
4	1.8833807452839535	4.386368224675985
5	1.0347853777309413	3.012501396494055
6	0.6879622971960743	2.4033822962395672
7	0.466230527043938	1.9002280102207862
8	0.3265160672574619	1.5209029887882444
9	0.2543312013984836	1.3327513207782153
>10	1.3512687627826734	13.936845113783242
>50	0.063799316519534	2.4981476783258723
>100	0.017280942087029523	1.6349398142212015
>500	0.0011027055832278773	0.4345779879383771
>1k	6.515987477199881E-4	0.5322566171830319
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	2.9101186571781277E-5
3	0.0	0.0	0.0	0.0	2.9101186571781277E-5
4	0.0	0.0	0.0	0.0	2.9101186571781277E-5
5	0.0	0.0	0.0	0.0	5.8202373143562554E-5
6	0.0	0.0	0.0	0.0	5.8202373143562554E-5
7	0.0	0.0	0.0	0.0	5.8202373143562554E-5
8	0.0	0.0	0.0	0.0	5.8202373143562554E-5
9	0.0	0.0	0.0	0.0	8.730355971534383E-5
10-11	0.0	0.0	0.0	0.0	8.730355971534383E-5
12-13	1.4550593285890638E-5	0.0	0.0	0.0	1.1640474628712511E-4
14-15	2.9101186571781277E-5	1.4550593285890638E-5	0.0	0.0	2.1825889928835958E-4
16-17	2.9101186571781277E-5	2.9101186571781277E-5	0.0	0.0	2.619106791460315E-4
18-19	2.9101186571781277E-5	2.9101186571781277E-5	0.0	0.0	2.619106791460315E-4
20-21	2.9101186571781277E-5	2.9101186571781277E-5	0.0	0.0	2.9101186571781274E-4
22-23	2.9101186571781277E-5	2.9101186571781277E-5	0.0	7.275296642945319E-5	3.6376483214726595E-4
24-25	2.9101186571781277E-5	2.9101186571781277E-5	0.0	2.4736008586014085E-4	4.074166120049379E-4
26-27	2.9101186571781277E-5	2.9101186571781277E-5	0.0	4.656189851485005E-4	4.2196720529082853E-4
28-29	2.9101186571781277E-5	2.9101186571781277E-5	0.0	0.0025609044183167525	4.3651779857671916E-4
30-31	2.9101186571781277E-5	2.9101186571781277E-5	0.0	0.011494968695853605	4.510683918626098E-4
32-33	5.8202373143562554E-5	2.9101186571781277E-5	0.0	0.03301529616568586	5.383719515779536E-4
34-35	5.8202373143562554E-5	2.9101186571781277E-5	0.0	0.0631059230809077	5.529225448638443E-4
36-37	5.8202373143562554E-5	5.8202373143562554E-5	0.0	0.1105117560063394	5.529225448638443E-4
38-39	5.8202373143562554E-5	8.730355971534383E-5	0.0	0.19110749221688766	5.529225448638443E-4
40-41	5.8202373143562554E-5	8.730355971534383E-5	0.0	0.2837220184815816	5.529225448638443E-4
42-43	5.8202373143562554E-5	1.1640474628712511E-4	0.0	0.38167661248219736	5.529225448638443E-4
44-45	5.8202373143562554E-5	1.1640474628712511E-4	0.0	0.49921630504562187	5.674731381497349E-4
46-47	5.8202373143562554E-5	1.3095533957301574E-4	0.0	0.6368503669368615	6.111249180074069E-4
48-49	5.8202373143562554E-5	1.8915771271657832E-4	0.0	0.7813232076724697	6.402261045791881E-4
50-51	5.8202373143562554E-5	2.1825889928835958E-4	0.0	0.9363743297269203	6.402261045791881E-4
52-53	5.8202373143562554E-5	2.3280949257425021E-4	0.0	1.0953104601887038	7.566308508663133E-4
54-55	5.8202373143562554E-5	2.4736008586014085E-4	0.0	1.2763052900718974	8.875861904393289E-4
56-57	5.8202373143562554E-5	2.619106791460315E-4	0.0	1.483767649142126	9.166873770111103E-4
58-59	5.8202373143562554E-5	2.619106791460315E-4	0.0	1.7048784647145203	9.312379702970009E-4
60-61	5.8202373143562554E-5	2.910118657178128E-4	0.0	1.9400597039943708	9.312379702970009E-4
62-63	5.8202373143562554E-5	2.910118657178128E-4	0.0	2.185935629339351	9.603391568687821E-4
64-65	5.8202373143562554E-5	2.910118657178128E-4	0.0	2.446158439664219	9.894403434405634E-4
66-67	5.8202373143562554E-5	3.7831542543315664E-4	0.0	2.749363702555608	0.001003990936726454
68-69	5.8202373143562554E-5	4.074166120049379E-4	0.0	3.0797931254848985	0.0010185415300123448
70-71	5.8202373143562554E-5	4.3651779857671916E-4	0.0	3.425107805345655	0.0010185415300123448
72-73	5.8202373143562554E-5	4.6561898514850043E-4	0.0	3.784842123152729	0.0011349462762994698
74-75	5.8202373143562554E-5	4.6561898514850043E-4	0.0	4.1683230092023775	0.0011640474628712512
76-77	5.8202373143562554E-5	4.6561898514850043E-4	0.0	4.589926449661059	0.0011640474628712512
78-79	8.730355971534383E-5	4.6561898514850043E-4	0.0	5.061904044075494	0.0011640474628712512
80-81	8.730355971534383E-5	4.6561898514850043E-4	0.0	5.551298698653139	0.0011785980561571418
82-83	8.730355971534383E-5	4.8016957843439106E-4	0.0	6.058081312207424	0.0012222498360148137
84-85	8.730355971534383E-5	4.947201717202817E-4	0.0	6.592146288172755	0.0012222498360148137
86-87	8.730355971534383E-5	4.947201717202817E-4	0.0	7.158440828266332	0.0012222498360148137
88-89	8.730355971534383E-5	4.947201717202817E-4	0.0	7.761577470559784	0.0012368004293007043
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2515	0.0	46.235973	1
GTATCAA	4455	0.0	43.041317	1
TCAACGC	5285	0.0	35.564342	4
CAACGCA	5505	0.0	34.05436	5
ATCAACG	5655	0.0	33.32134	3
TATCAAC	5680	0.0	32.92447	2
AACGCAG	6130	0.0	30.9518	6
GTACATG	9220	0.0	28.51877	1
TACATGG	9595	0.0	27.009626	2
ACATGGG	10030	0.0	25.553915	3
ACGCAGA	7145	0.0	25.429987	7
CGTATAC	230	1.364242E-10	24.76384	3
CGCAGAG	7450	0.0	24.072252	8
CATGGGG	8585	0.0	23.110031	4
GCAGAGT	8505	0.0	19.525127	9
ATGGGGG	5110	0.0	18.668446	5
AGAGTAC	7025	0.0	18.235895	10-11
GAGTACA	6840	0.0	17.555628	1
TAAGGTA	930	0.0	17.35244	4
TAATACA	1180	0.0	17.296213	4
>>END_MODULE