FastQCFastQC Report
Mon 15 Aug 2016
SRR2921712_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921712_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3436286
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24550.048.7967261
GTATCAA46900.044.902711
TCAACGC58700.035.0087474
CAACGCA60800.033.7504845
TATCAAC61150.033.6061062
ATCAACG62950.032.645173
AACGCAG64700.031.7843936
ACGCAGA78600.025.8613687
CGCAGAG82250.024.3095268
GTACATG99850.024.1380141
TACATGG104950.022.5654952
ACATGGG109250.021.1994763
GCAGAGT96250.019.9347559
CATGGGG93800.019.125634
AGAGTAC81400.018.40794210-11
TAAGGTG16800.017.8127565
GAGTACA79550.017.1507591
GTATACG1700.001778978416.7781451
ATGGGGG52150.016.4863365
CAGAGTA89000.016.27571710-11
Produced by FastQC (version 0.11.5)