##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921712_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3436286
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.313963389543247	33.0	31.0	34.0	28.0	34.0
2	31.453460218386944	34.0	31.0	34.0	28.0	34.0
3	31.52644133811912	34.0	31.0	34.0	28.0	34.0
4	35.00050927076501	37.0	35.0	37.0	32.0	37.0
5	34.73258977861563	37.0	35.0	37.0	32.0	37.0
6	34.70354213822714	37.0	35.0	37.0	32.0	37.0
7	34.58828368767908	37.0	35.0	37.0	32.0	37.0
8	34.53170865288861	37.0	35.0	37.0	32.0	37.0
9	36.11698240484058	39.0	37.0	39.0	32.0	39.0
10-11	36.00768285876089	39.0	37.0	39.0	31.0	39.0
12-13	35.92503374282583	39.0	37.0	39.0	31.0	39.0
14-15	37.16039671901582	40.0	38.0	41.0	31.0	41.0
16-17	37.123996227322166	40.0	38.0	41.0	31.0	41.0
18-19	37.0782392676279	40.0	38.0	41.0	31.0	41.0
20-21	37.01676897673826	40.0	38.0	41.0	31.0	41.0
22-23	36.92488619980991	40.0	37.0	41.0	30.0	41.0
24-25	36.83673332196447	40.0	37.0	41.0	30.0	41.0
26-27	36.70565182874766	40.0	37.0	41.0	30.0	41.0
28-29	36.58322342785205	40.0	37.0	41.0	30.0	41.0
30-31	36.42697842961849	40.0	37.0	41.0	30.0	41.0
32-33	36.37849599829583	40.0	36.5	41.0	29.5	41.0
34-35	36.50801330273441	40.0	37.0	41.0	30.0	41.0
36-37	36.50250095597398	40.0	37.0	41.0	30.0	41.0
38-39	36.394100927571216	40.0	37.0	41.0	29.5	41.0
40-41	36.31059943788148	40.0	37.0	41.0	29.0	41.0
42-43	36.18498576660965	40.0	36.0	41.0	29.0	41.0
44-45	36.05119582595861	40.0	36.0	41.0	28.0	41.0
46-47	35.89022683210885	40.0	36.0	41.0	28.0	41.0
48-49	35.74225733830071	40.0	35.0	41.0	27.0	41.0
50-51	35.580165475167085	39.5	35.0	41.0	27.0	41.0
52-53	35.39682538065807	39.0	35.0	41.0	26.5	41.0
54-55	35.17497583146455	39.0	35.0	41.0	26.0	41.0
56-57	34.94142003896067	39.0	35.0	41.0	26.0	41.0
58-59	34.69537227110898	38.0	35.0	40.0	26.0	41.0
60-61	34.409674573071044	38.0	34.0	40.0	25.0	41.0
62-63	34.100467481461095	37.0	34.0	40.0	24.5	41.0
64-65	33.757152053117814	37.0	34.0	40.0	23.0	41.0
66-67	33.404780335513394	36.0	34.0	39.0	22.5	41.0
68-69	33.040070442332215	36.0	33.0	39.0	22.0	40.5
70-71	32.64329598875065	35.0	33.0	38.5	21.5	40.0
72-73	32.28618412437149	35.0	33.0	37.0	20.0	39.5
74-75	31.886569685992377	35.0	33.0	37.0	19.5	39.0
76-77	29.209494494928535	33.0	29.0	34.5	12.5	36.5
78-79	30.775226072567882	34.5	31.5	35.5	14.0	37.0
80-81	30.961715497487695	35.0	32.0	35.0	16.5	37.0
82-83	30.793066409489782	35.0	32.0	35.0	13.5	37.0
84-85	30.603358538841064	35.0	32.0	35.0	10.5	36.0
86-87	30.403316254816975	35.0	32.0	35.0	7.0	36.0
88-89	30.144740280640203	35.0	31.5	35.0	2.0	35.5
90-91	29.950986326516478	34.0	31.0	35.0	2.0	35.0
92-93	29.795771219275693	34.0	31.0	35.0	2.0	35.0
94-95	29.691694754161908	34.0	31.0	35.0	2.0	35.0
96-97	29.487102208605457	34.0	31.0	35.0	2.0	35.0
98-99	29.28651864251113	34.0	31.0	35.0	2.0	35.0
100-101	28.176272871350058	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69726.0
3	23515.0
4	12770.0
5	8965.0
6	7795.0
7	7758.0
8	8141.0
9	8776.0
10	9754.0
11	10463.0
12	10890.0
13	11670.0
14	12340.0
15	13328.0
16	14009.0
17	14625.0
18	14008.0
19	13176.0
20	12568.0
21	12402.0
22	13289.0
23	14786.0
24	17695.0
25	20925.0
26	25746.0
27	30872.0
28	37091.0
29	44723.0
30	54770.0
31	67668.0
32	86214.0
33	113569.0
34	162650.0
35	245164.0
36	434874.0
37	835453.0
38	838274.0
39	95841.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.06642640149663	19.251402210773804	13.348008863616093	26.33416252411348
2	15.23108406120336	20.80795464400186	37.161472777845354	26.79948851694942
3	17.842136588333048	24.635988061508552	28.840799256099853	28.68107609405855
4	13.208208299974128	16.61665332298434	35.0445259907866	35.13061238625493
5	14.387971334260913	37.12344198447951	34.08914672270962	14.399439958549948
6	34.03774087871531	35.18656105064255	16.759108334417373	14.016589736224766
7	29.302042961499712	31.41778652882793	20.930359114462533	18.349811395209827
8	27.0943105434181	34.40845727043674	19.727781680570246	18.769450505574913
9	26.93476441411711	15.315148573284395	18.906790009207622	38.84329700339087
10-11	25.904828342393433	25.099318505457752	27.58818420587558	21.407668946273237
12-13	26.417153943911874	22.392980646753415	27.409435984530905	23.780429424803806
14-15	23.555339678395093	23.86700020270012	24.55570040602594	28.021959712878854
16-17	24.006805603491678	26.866375499594618	25.306522798160575	23.82029609875313
18-19	24.028369430021353	26.355466478708962	26.421017893426352	23.195146197843336
20-21	24.590050868962948	25.53496804684135	26.285488958990538	23.589492125205165
22-23	24.384640859346398	25.324798343327654	26.06884293100167	24.221717866324283
24-25	24.222288767264725	25.54126292208206	26.010420022413843	24.22602828823937
26-27	24.000790410079066	25.733683022858738	26.04936687163199	24.2161596954302
28-29	24.17204690362749	25.91842605714399	25.825981389708332	24.08354564952019
30-31	24.39156466774688	25.663634519385788	25.682407773116644	24.26239303975069
32-33	24.20556384710131	25.732880157024546	25.565238295920484	24.496317699953664
34-35	24.477824009741745	25.746605472252167	25.710239819763736	24.065330698242356
36-37	24.57201460818001	25.582591249468518	25.760828157735517	24.084565984615953
38-39	24.379984669494913	25.69368498431155	25.688606827254773	24.23772351893876
40-41	24.527908183807583	25.527218607813147	25.72038428255649	24.22448892582278
42-43	24.402837404542606	25.513517776852275	25.58651054131353	24.497134277291597
44-45	24.330507952939655	25.525336966956157	25.720342491457863	24.423812588646314
46-47	24.469692229564064	25.571225479568067	25.548569408046472	24.4105128828214
48-49	24.24556478868218	25.612402220898005	25.62199186699059	24.520041123429223
50-51	24.26801761655573	25.67254898907936	25.68354951451451	24.3758838798504
52-53	24.520424362270695	25.508764730961246	25.586524708145447	24.38428619862261
54-55	24.065934001972565	25.443907438972822	25.801751562002984	24.688406997051626
56-57	24.076698261691558	25.572514307400734	25.710175018277155	24.64061241263056
58-59	24.212616730963987	25.443067946374736	25.687615577643363	24.65669974501791
60-61	24.154503467463094	25.469908660137687	25.88867884250164	24.486909029897582
62-63	24.15350458771675	25.5761079250451	25.768567172628988	24.501820314609162
64-65	23.97479063768711	25.377012287344225	25.715005233148027	24.93319184182064
66-67	23.977071706969777	25.50693978338383	25.655126325460042	24.86086218418635
68-69	24.055274179906043	25.465706220528954	25.64999716117123	24.82902243839378
70-71	24.01778790165255	25.31996352842404	25.628125630659188	25.034122939264225
72-73	23.970731544010032	25.53469006338248	25.486918493579875	25.00765989902761
74-75	23.906912446018232	25.629482227698368	25.505126402951007	24.958478923332393
76-77	23.973520403006948	25.283747989114275	25.66156335673649	25.081168251142287
78-79	23.831901052475217	25.383759522446837	25.67513802177766	25.10920140330029
80-81	24.024343348554357	25.542679413673415	25.417328379086925	25.015648858685307
82-83	23.926065878060484	25.568250705214666	25.504835601765958	25.00084781495889
84-85	23.856255156356525	25.578760302340285	25.450167121636802	25.114817419666384
86-87	23.769909138641545	25.724923724043098	25.389795839323824	25.115371297991533
88-89	23.665466145597716	25.513149791757268	25.464627921162542	25.356756141482478
90-91	23.595062892994566	25.53093805879528	25.49959116233504	25.374407885875115
92-93	23.69413007468686	25.404538047030794	25.565144929076457	25.336186949205892
94-95	23.5358831413192	25.349649077619112	25.579833204547803	25.534634576513888
96-97	23.431967887195963	25.25972756855936	25.650950654781678	25.657353889462996
98-99	23.313436184659782	25.288610025828994	25.544775568864296	25.853178220646928
100-101	23.25452131637778	25.30413720892198	25.57784905645003	25.86349241825021
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	43.0
1	39.0
2	56.0
3	82.0
4	84.0
5	90.5
6	137.0
7	299.5
8	459.0
9	477.5
10	412.0
11	350.5
12	347.0
13	382.0
14	423.5
15	485.5
16	565.0
17	621.5
18	696.0
19	795.5
20	899.0
21	1023.0
22	1212.0
23	1470.5
24	1796.5
25	2245.0
26	2885.0
27	3797.0
28	5214.0
29	7414.5
30	10714.5
31	15768.0
32	22791.0
33	28716.5
34	33890.0
35	41036.5
36	50259.5
37	59850.5
38	69230.0
39	78229.5
40	86740.0
41	95836.5
42	104874.5
43	113790.5
44	121863.5
45	131017.0
46	139170.0
47	145487.5
48	151847.0
49	155151.0
50	155227.0
51	155394.0
52	155475.5
53	154730.0
54	151952.0
55	144392.0
56	133919.5
57	121470.0
58	110354.5
59	97122.5
60	80567.5
61	67246.5
62	55672.0
63	42717.5
64	30502.5
65	22049.5
66	16435.5
67	12842.0
68	9968.0
69	7434.5
70	5697.5
71	4192.0
72	2902.5
73	2170.0
74	1631.0
75	1159.5
76	797.0
77	492.0
78	279.5
79	165.0
80	106.0
81	59.5
82	29.0
83	20.5
84	17.0
85	11.0
86	8.5
87	7.0
88	4.5
89	4.0
90	4.0
91	4.5
92	3.0
93	1.0
94	1.5
95	1.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16389788277227216
2	1.1640474628712511E-4
3	0.009923504620977417
4	0.0059075408740716
5	0.024037580108291337
6	0.0018915771271657833
7	0.0
8	0.0
9	5.8202373143562554E-5
10-11	0.047405832925431705
12-13	4.3651779857671916E-4
14-15	0.0051072582433476146
16-17	0.0
18-19	0.0015132617017326266
20-21	1.7460711943068766E-4
22-23	0.0
24-25	4.947201717202817E-4
26-27	0.0027646127243192214
28-29	0.005136359429919395
30-31	0.01591834905476436
32-33	0.011349462762994698
34-35	0.010229067079981119
36-37	0.003754053067759785
38-39	0.0
40-41	0.003390288235612519
42-43	0.009574290382116041
44-45	0.006678722318223803
46-47	0.003521243575185535
48-49	0.008395692325958898
50-51	0.0025172526384590805
52-53	0.0020661842465964707
54-55	0.004452481545482536
56-57	0.008788558344677946
58-59	0.01613660795405272
60-61	0.007930073340810398
62-63	0.021927744081837192
64-65	0.042225821715654636
66-67	0.06114159298731246
68-69	0.05153820141862465
70-71	0.06923172285426765
72-73	0.03458676024056205
74-75	0.028591915806775104
76-77	0.22581065720373683
78-79	0.08587760157332655
80-81	0.06779121411896448
82-83	0.02865011817991867
84-85	0.05271679947478178
86-87	0.06905711573483697
88-89	0.2908954609715257
90-91	0.2941984456474228
92-93	0.052760451254639455
94-95	0.009923504620977417
96-97	0.015205369983755719
98-99	9.166873770111102E-4
100-101	2.3280949257425021E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3436286.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.2506091002321
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.96461091476901	44.06270854032897
2	12.51018303564178	14.32431197491765
3	4.197617974408526	7.209485574149116
4	1.9320960971698005	4.424547136126091
5	1.0884153939349839	3.1156222128423443
6	0.7274528808765003	2.498827231313893
7	0.47245378525930576	1.8933786884463857
8	0.3445021784912806	1.5778367643986153
9	0.2533516763500513	1.3054084008844773
>10	1.421197839167696	14.370306143815615
>50	0.06816243181311765	2.592825037948174
>100	0.018119309623637864	1.6493083475471841
>500	0.0012753350700358906	0.511504842157247
>1k	5.61147424210533E-4	0.4639291051242684
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.730355971534383E-5
2	0.0	0.0	0.0	2.9101186571781277E-5	8.730355971534383E-5
3	0.0	0.0	0.0	2.9101186571781277E-5	8.730355971534383E-5
4	0.0	0.0	0.0	5.8202373143562554E-5	8.730355971534383E-5
5	0.0	2.9101186571781277E-5	0.0	5.8202373143562554E-5	8.730355971534383E-5
6	0.0	2.9101186571781277E-5	0.0	5.8202373143562554E-5	1.1640474628712511E-4
7	0.0	2.9101186571781277E-5	0.0	5.8202373143562554E-5	1.1640474628712511E-4
8	0.0	2.9101186571781277E-5	0.0	5.8202373143562554E-5	1.1640474628712511E-4
9	0.0	2.9101186571781277E-5	0.0	8.730355971534383E-5	1.1640474628712511E-4
10-11	0.0	2.9101186571781277E-5	0.0	1.1640474628712511E-4	1.1640474628712511E-4
12-13	0.0	2.9101186571781277E-5	0.0	1.1640474628712511E-4	1.8915771271657832E-4
14-15	0.0	7.275296642945319E-5	0.0	1.1640474628712511E-4	2.3280949257425021E-4
16-17	0.0	8.730355971534383E-5	0.0	1.1640474628712511E-4	3.346636455754847E-4
18-19	0.0	8.730355971534383E-5	0.0	1.1640474628712511E-4	3.492142388613753E-4
20-21	0.0	8.730355971534383E-5	0.0	1.1640474628712511E-4	4.074166120049379E-4
22-23	0.0	8.730355971534383E-5	0.0	1.8915771271657832E-4	4.8016957843439106E-4
24-25	0.0	1.0185415300123448E-4	0.0	3.7831542543315664E-4	5.23821358292063E-4
26-27	0.0	1.1640474628712511E-4	0.0	6.256755112932974E-4	5.23821358292063E-4
28-29	0.0	1.3095533957301574E-4	0.0	0.0026773091646038775	5.23821358292063E-4
30-31	0.0	1.455059328589064E-4	0.0	0.011902385307858542	5.23821358292063E-4
32-33	0.0	1.455059328589064E-4	0.0	0.034324849561416015	5.529225448638443E-4
34-35	1.4550593285890638E-5	1.455059328589064E-4	0.0	0.06467738715578389	5.529225448638443E-4
36-37	2.9101186571781277E-5	2.0370830600246895E-4	0.0	0.11259249084622176	5.529225448638443E-4
38-39	2.9101186571781277E-5	2.9101186571781274E-4	0.0	0.19427952155321182	5.820237314356256E-4
40-41	2.9101186571781277E-5	3.346636455754847E-4	0.0	0.28712685731047993	5.820237314356256E-4
42-43	2.9101186571781277E-5	3.7831542543315664E-4	0.0	0.38576532919553264	5.820237314356256E-4
44-45	2.9101186571781277E-5	3.7831542543315664E-4	0.0	0.5033195723522431	5.820237314356256E-4
46-47	2.9101186571781277E-5	3.7831542543315664E-4	0.0	0.64156475916149	5.820237314356256E-4
48-49	2.9101186571781277E-5	3.7831542543315664E-4	0.0	0.786736028374821	6.111249180074069E-4
50-51	2.9101186571781277E-5	3.9286601871904727E-4	0.0	0.9415252397501255	6.984284777227506E-4
52-53	2.9101186571781277E-5	4.074166120049379E-4	0.0	1.0987589507974598	7.420802575804226E-4
54-55	2.9101186571781277E-5	4.074166120049379E-4	0.0	1.279710128900796	7.857320374380945E-4
56-57	2.9101186571781277E-5	4.074166120049379E-4	0.0	1.4866777677993042	8.148332240098758E-4
58-59	2.9101186571781277E-5	4.074166120049379E-4	0.0	1.7081232470172738	8.148332240098758E-4
60-61	2.9101186571781277E-5	4.074166120049379E-4	0.0	1.9436828017225576	8.439344105816571E-4
62-63	2.9101186571781277E-5	4.074166120049379E-4	0.0	2.190402661478119	8.730355971534383E-4
64-65	2.9101186571781277E-5	4.074166120049379E-4	0.0	2.4495341773065453	8.730355971534383E-4
66-67	2.9101186571781277E-5	4.3651779857671916E-4	0.0	2.7516772468880646	8.730355971534383E-4
68-69	2.9101186571781277E-5	4.3651779857671916E-4	0.0	3.082630491175647	8.730355971534383E-4
70-71	2.9101186571781277E-5	4.3651779857671916E-4	0.0	3.4294293315515647	8.875861904393289E-4
72-73	4.3651779857671915E-5	4.3651779857671916E-4	0.0	3.791258934791807	9.894403434405634E-4
74-75	5.8202373143562554E-5	4.3651779857671916E-4	0.0	4.1792505047600805	0.0010185415300123448
76-77	5.8202373143562554E-5	4.947201717202817E-4	0.0	4.603327546077364	0.001047642716584126
78-79	5.8202373143562554E-5	4.947201717202817E-4	0.0	5.078244360335548	0.0010912944964417979
80-81	5.8202373143562554E-5	4.947201717202817E-4	0.0	5.572309755357965	0.0011058450897276887
82-83	5.8202373143562554E-5	4.947201717202817E-4	0.0	6.083384793931588	0.0011640474628712512
84-85	5.8202373143562554E-5	4.947201717202817E-4	0.0	6.621451183050538	0.0011931486494430323
86-87	5.8202373143562554E-5	5.23821358292063E-4	0.0	7.1912087643461575	0.0012368004293007043
88-89	5.8202373143562554E-5	5.23821358292063E-4	0.0	7.799816429715105	0.0012804522091583762
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2455	0.0	48.796726	1
GTATCAA	4690	0.0	44.90271	1
TCAACGC	5870	0.0	35.008747	4
CAACGCA	6080	0.0	33.750484	5
TATCAAC	6115	0.0	33.606106	2
ATCAACG	6295	0.0	32.64517	3
AACGCAG	6470	0.0	31.784393	6
ACGCAGA	7860	0.0	25.861368	7
CGCAGAG	8225	0.0	24.309526	8
GTACATG	9985	0.0	24.138014	1
TACATGG	10495	0.0	22.565495	2
ACATGGG	10925	0.0	21.199476	3
GCAGAGT	9625	0.0	19.934755	9
CATGGGG	9380	0.0	19.12563	4
AGAGTAC	8140	0.0	18.407942	10-11
TAAGGTG	1680	0.0	17.812756	5
GAGTACA	7955	0.0	17.150759	1
GTATACG	170	0.0017789784	16.778145	1
ATGGGGG	5215	0.0	16.486336	5
CAGAGTA	8900	0.0	16.275717	10-11
>>END_MODULE