FastQCFastQC Report
Mon 15 Aug 2016
SRR2921711_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921711_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2909394
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.054.1011961
GTATCAA47600.046.963851
TCAACGC59750.036.546384
CAACGCA61300.035.619225
ATCAACG62650.035.080793
TATCAAC64200.034.5289882
AACGCAG65300.033.5636676
GTACATG101550.030.8003181
TACATGG106650.028.7078632
ACGCAGA76800.027.8009197
ACATGGG109850.026.9646023
CGCAGAG77900.026.495658
CATGGGG89800.024.9511224
ATGGGGG58600.021.8702245
GCAGAGT91900.021.6332249
GAGTACA72950.020.4943891
GTATAGG8450.020.2206671
AGAGTAC78350.018.89478910-11
TAAGGTG14450.018.7238035
TAAGGTA7950.018.5105364
Produced by FastQC (version 0.11.5)