##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921711_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2909394
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.283116690279833	31.0	31.0	34.0	26.0	34.0
2	30.33277273549062	31.0	31.0	34.0	26.0	34.0
3	30.389709334658694	33.0	31.0	34.0	26.0	34.0
4	33.8515168450887	37.0	35.0	37.0	30.0	37.0
5	33.84912975004417	37.0	35.0	37.0	30.0	37.0
6	33.88844687244148	37.0	35.0	37.0	30.0	37.0
7	33.80119261949395	37.0	35.0	37.0	29.0	37.0
8	33.77411790909035	37.0	35.0	37.0	29.0	37.0
9	35.32083829141051	39.0	35.0	39.0	29.0	39.0
10-11	35.21431988929653	39.0	35.0	39.0	29.0	39.0
12-13	35.09536573595739	39.0	35.0	39.0	28.0	39.0
14-15	36.20804624605674	40.0	36.0	41.0	27.0	41.0
16-17	36.1500027153421	40.0	36.0	41.0	27.0	41.0
18-19	36.118764938677955	40.0	36.0	41.0	27.0	41.0
20-21	36.042775058998544	40.0	36.0	41.0	27.0	41.0
22-23	35.94568078438328	40.0	36.0	41.0	27.0	41.0
24-25	35.831040931547946	40.0	36.0	41.0	27.0	41.0
26-27	35.675994210478194	40.0	36.0	41.0	26.5	41.0
28-29	35.537994166482775	40.0	36.0	41.0	25.5	41.0
30-31	35.38276493317852	39.5	35.5	41.0	25.0	41.0
32-33	35.197708698100016	39.0	35.0	41.0	24.5	41.0
34-35	35.02801030042683	39.0	35.0	41.0	24.0	41.0
36-37	34.99105861908012	39.0	35.0	41.0	24.0	41.0
38-39	35.16774816336323	39.5	35.0	41.0	24.0	41.0
40-41	35.22442732060353	40.0	35.0	41.0	24.0	41.0
42-43	35.06096475760931	40.0	35.0	41.0	23.0	41.0
44-45	34.93832667558949	40.0	35.0	41.0	23.0	41.0
46-47	34.77635016089261	39.5	35.0	41.0	21.5	41.0
48-49	34.60791027272346	39.0	35.0	41.0	20.0	41.0
50-51	32.91942480117853	37.0	32.5	39.5	16.5	40.0
52-53	33.03410469671691	37.5	32.5	39.5	14.5	40.0
54-55	33.94297369142852	38.5	34.0	40.5	17.0	41.0
56-57	33.96841679057563	38.5	34.0	41.0	16.0	41.0
58-59	33.77993338131583	38.0	34.0	41.0	13.5	41.0
60-61	33.525385527020404	38.0	34.0	40.0	12.5	41.0
62-63	33.23228084611435	37.0	33.0	40.0	8.5	41.0
64-65	32.94843341946811	37.0	33.0	40.0	7.5	41.0
66-67	32.61198414515188	36.0	33.0	39.5	4.5	41.0
68-69	32.25244810431313	36.0	32.5	39.0	2.0	41.0
70-71	31.848225781726367	35.0	32.0	39.0	2.0	40.5
72-73	31.496137339940894	35.0	32.0	37.5	2.0	40.0
74-75	31.097415991096426	35.0	32.0	37.0	2.0	39.0
76-77	30.725928492325206	35.0	31.0	36.5	2.0	39.0
78-79	30.381511579387322	35.0	31.0	36.0	2.0	38.5
80-81	30.070534448067193	35.0	31.0	36.0	2.0	37.0
82-83	29.756116565855294	35.0	31.0	35.0	2.0	37.0
84-85	29.49138119484676	35.0	31.0	35.0	2.0	36.5
86-87	29.25626281624283	35.0	30.0	35.0	2.0	36.0
88-89	29.030219007807126	34.5	30.0	35.0	2.0	36.0
90-91	28.805370121750443	34.0	30.0	35.0	2.0	35.5
92-93	28.6079635484228	34.0	30.0	35.0	2.0	35.0
94-95	28.37117128171709	34.0	29.5	35.0	2.0	35.0
96-97	28.13022333860591	34.0	29.0	35.0	2.0	35.0
98-99	27.856778765612358	34.0	29.0	35.0	2.0	35.0
100-101	26.782887948486866	33.0	26.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	105717.0
3	18031.0
4	12473.0
5	9469.0
6	8546.0
7	8646.0
8	9251.0
9	9714.0
10	10625.0
11	11388.0
12	11358.0
13	11464.0
14	12102.0
15	13285.0
16	13303.0
17	13840.0
18	13446.0
19	12268.0
20	12432.0
21	13038.0
22	14299.0
23	16405.0
24	19358.0
25	22476.0
26	26794.0
27	31663.0
28	37237.0
29	44340.0
30	52878.0
31	64319.0
32	79869.0
33	103141.0
34	146152.0
35	208106.0
36	340577.0
37	610126.0
38	646805.0
39	114439.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.09546572209708	18.190449988823357	12.851866499303611	24.86221778977595
2	16.04430598537374	19.874678259804757	39.07290668081068	25.00810907401082
3	18.87318963581012	24.46187379121312	29.40010463111341	27.26483194186335
4	13.29249895265592	15.740463665587335	36.8992561621323	34.06778121962444
5	14.186030754393741	37.56388248683485	34.159430463489606	14.0906562952818
6	33.179964284548	35.227604366291345	17.49472775991241	14.097703589248239
7	28.82248014022587	31.993581345751714	21.338106531372585	17.84583198264983
8	27.04762350026006	34.21883921907814	20.27593736646681	18.45759991419499
9	27.53128312725403	15.264832416483229	19.337138685208075	37.86674577105467
10-11	25.75309700411238	25.168656396774036	27.894057829077205	21.18418877003638
12-13	26.30195105508035	22.652766583143087	27.722144096134453	23.323138265642108
14-15	23.586127953120737	24.117257293679987	25.000885416147305	27.295729337051966
16-17	23.70936757863903	27.08012126014978	25.803709487910513	23.406801673300677
18-19	23.71832485821231	26.653972031928742	26.99333780893973	22.634365300919217
20-21	24.232601927972862	25.837951063434435	26.96076552359189	22.968681485000808
22-23	24.200928706876248	25.485559656780747	26.61520555504935	23.69830608129366
24-25	23.947935152917665	25.755605766240436	26.535928633207504	23.760530447634387
26-27	23.613643430786986	26.018256079128584	26.587944208311065	23.780156281773365
28-29	23.740123693493278	26.28578686720438	26.352148840573307	23.621940598729033
30-31	24.2033065720655	25.925635644564988	26.213495447834433	23.65756233553508
32-33	23.933621023916313	26.00871596593362	26.07648504164799	23.981177968502074
34-35	24.26412886668988	26.128533956818263	26.19652424568762	23.41081293080424
36-37	24.250792696025776	25.92793984962406	26.30245327604726	23.5188141783029
38-39	24.087321528865765	26.09287682853641	26.13372866406764	23.686072978530188
40-41	24.236063885604946	25.808542198248997	26.255067056924787	23.700326859221274
42-43	23.998050976371964	25.848383323136957	26.074236511847086	24.079329188643992
44-45	23.801353145582215	25.901547492592965	26.318945019614638	23.978154342210185
46-47	24.03165568663632	25.83342072242452	26.0151555930482	24.119767997890953
48-49	23.845605300259464	26.00081178433952	26.057364600864375	24.096218314536642
50-51	23.88554488643134	25.942226933442985	26.278211361618563	23.894016818507108
52-53	24.161299310592803	25.748792237254992	26.22872875544729	23.86117969670492
54-55	23.47069716599274	25.6574449817418	26.662274458806174	24.209583393459283
56-57	23.680802715025006	25.77019616703162	26.321074300183295	24.227926817760075
58-59	23.903911516592704	25.676862107019403	26.369172024892844	24.050054351495046
60-61	23.87910149350948	25.79280313709497	26.498284390424274	23.829810978971274
62-63	23.8969089065591	25.683331680194847	26.566400373609294	23.85335903963675
64-65	23.60890766104931	25.506403192874476	26.489272505885385	24.395416640190824
66-67	23.69689195767595	25.696241395942238	26.280059421874817	24.326807224506993
68-69	23.714893145884414	25.65032558250056	26.422280770116213	24.212500501498816
70-71	23.704959947658626	25.43012469815471	26.329638732615646	24.53527662157102
72-73	23.651944430047063	25.74546917260322	26.143634749077165	24.458951648272553
74-75	23.614702055107653	25.85477955578652	26.26862849730681	24.26188989179901
76-77	23.68428529046684	25.397327737120328	26.34933365612114	24.569053316291697
78-79	23.615139463363207	25.440547031579413	26.411260810249022	24.53305269480836
80-81	23.89714246937782	25.690063685732774	26.06161689803273	24.35117694685668
82-83	23.874698084689225	25.64432137429507	26.278326304143253	24.202654236872455
84-85	23.817208819475205	25.621430357572173	26.224674525764115	24.33668629718851
86-87	23.66448123840093	25.850558322891942	26.133052859895457	24.35190757881167
88-89	23.65235807410485	25.517931642427456	26.249396732486126	24.580313550981572
90-91	23.781917798969562	25.373202200189855	26.37239279517575	24.472487205664837
92-93	24.030770084031936	25.151017892720155	26.41565656504763	24.402555458200283
94-95	23.87244575430667	25.124665620537662	26.40923395452267	24.593654670633
96-97	23.800764704787984	24.942636805190457	26.50083133567012	24.75576715435144
98-99	23.57958509157316	24.943269528562634	26.43777768820788	25.039367691656324
100-101	23.874882538730997	24.95081484314237	26.485257372605663	24.68904524552097
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	63.5
2	100.0
3	150.5
4	125.0
5	137.5
6	222.0
7	425.5
8	694.0
9	754.0
10	630.0
11	542.5
12	585.0
13	689.5
14	818.5
15	955.0
16	1026.5
17	1039.0
18	1179.5
19	1328.0
20	1419.0
21	1548.5
22	1670.5
23	1907.5
24	2182.0
25	2522.5
26	3238.0
27	4285.5
28	5694.0
29	7820.0
30	11272.5
31	16557.5
32	23995.5
33	30528.5
34	36107.0
35	43412.0
36	52974.5
37	62246.0
38	69672.5
39	77473.5
40	83362.5
41	88045.0
42	93310.0
43	99085.0
44	104554.5
45	110825.0
46	115836.0
47	119271.0
48	123039.0
49	124926.0
50	125453.0
51	124576.5
52	123347.0
53	122917.0
54	121411.0
55	115941.5
56	107341.0
57	96779.0
58	86844.0
59	75136.5
60	61010.5
61	50088.5
62	41911.0
63	32784.0
64	23533.0
65	17119.5
66	12934.5
67	9995.0
68	7522.5
69	5769.0
70	4486.0
71	3215.0
72	2252.0
73	1685.5
74	1352.0
75	1015.0
76	718.5
77	486.5
78	306.5
79	215.5
80	166.5
81	140.0
82	115.5
83	84.5
84	72.5
85	57.5
86	46.0
87	40.5
88	38.0
89	38.5
90	36.5
91	28.5
92	23.0
93	19.0
94	16.5
95	12.0
96	8.0
97	7.5
98	5.5
99	5.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05306947082450847
2	0.07413915062724402
3	0.07001458035590917
4	0.07011769461269253
5	0.06554629589529641
6	0.009933340070131443
7	0.012820539260065841
8	0.01625768115284489
9	0.005430684190590893
10-11	0.012820539260065841
12-13	0.021808665309683048
14-15	0.039767731699453565
16-17	0.02651754970279034
18-19	0.010002082907987023
20-21	0.020193208620076897
22-23	0.036055618455252195
24-25	0.03376991909655413
26-27	0.020949379836488286
28-29	0.011978439496334975
30-31	0.011651911016520966
32-33	0.008592854731947616
34-35	0.005327569933807522
36-37	6.530569596280187E-4
38-39	0.0034371418927790463
40-41	0.0012889282097921424
42-43	0.22004582397571454
44-45	0.19136287488047338
46-47	0.1963811020439308
48-49	0.2032553858294889
50-51	0.19550463086127215
52-53	0.20227580039004683
54-55	0.19821997295656757
56-57	0.20100405788971862
58-59	0.2086345128916881
60-61	0.21305124022390917
62-63	0.20024788667330723
64-65	0.19947452974743196
66-67	0.19761847312533123
68-69	0.1924283888672349
70-71	0.18496979095990437
72-73	0.1960030164357251
74-75	0.19514373096253035
76-77	0.1899192752855062
78-79	0.20674408485065965
80-81	0.2169008391438217
82-83	0.21523382532582389
84-85	0.20939068410809947
86-87	0.22970419269442363
88-89	0.22179876634103182
90-91	0.22838089306570372
92-93	0.223740751510452
94-95	0.22784813607232296
96-97	0.2349630197903756
98-99	0.22798562174803413
100-101	0.23515206259447843
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2909394.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.62506685581072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.75901662912094	44.03109515266071
2	11.889864846104603	13.465287836344537
3	4.115284155081808	6.9908472123649785
4	1.875615264355957	4.248273589597407
5	1.0768283191846897	3.048773778303167
6	0.6856115194605877	2.3293678875941852
7	0.46301589903417034	1.8352814366679244
8	0.3408532039549433	1.5440668369572763
9	0.25605315147936053	1.3049124139043837
>10	1.4295061411031056	14.500896801407567
>50	0.07867333712454676	2.9978010947657454
>100	0.02760458137623033	2.586963787122032
>500	0.0012803530884560827	0.5164077071873087
>1k	7.92599530754454E-4	0.6000244651228483
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.437141892779046E-5
5	0.0	0.0	0.0	0.0	3.437141892779046E-5
6	0.0	0.0	0.0	0.0	6.874283785558092E-5
7	0.0	0.0	0.0	0.0	6.874283785558092E-5
8	0.0	0.0	0.0	0.0	6.874283785558092E-5
9	0.0	0.0	0.0	0.0	1.0311425678337138E-4
10-11	0.0	0.0	0.0	0.0	1.0311425678337138E-4
12-13	1.718570946389523E-5	0.0	0.0	0.0	2.5778564195842843E-4
14-15	3.437141892779046E-5	0.0	0.0	0.0	3.952713176695903E-4
16-17	3.437141892779046E-5	0.0	0.0	5.155712839168569E-5	5.155712839168569E-4
18-19	3.437141892779046E-5	0.0	0.0	6.874283785558092E-5	5.843141217724378E-4
20-21	3.437141892779046E-5	0.0	0.0	1.0311425678337138E-4	6.186855407002283E-4
22-23	3.437141892779046E-5	0.0	0.0	2.0622851356674277E-4	6.874283785558092E-4
24-25	3.437141892779046E-5	0.0	0.0	5.155712839168569E-4	7.561712164113901E-4
26-27	3.437141892779046E-5	0.0	0.0	8.936568921225519E-4	8.936568921225519E-4
28-29	3.437141892779046E-5	0.0	0.0	0.0032309133792123032	9.623997299781329E-4
30-31	3.437141892779046E-5	0.0	0.0	0.015587438483752974	9.967711489059232E-4
32-33	3.437141892779046E-5	0.0	0.0	0.04392667338971621	0.0010311425678337137
34-35	3.437141892779046E-5	0.0	0.0	0.08063534880459641	0.0010655139867615042
36-37	3.437141892779046E-5	0.0	0.0	0.14047598915787962	0.0010998854056892948
38-39	3.437141892779046E-5	0.0	0.0	0.24345276026553986	0.0011342568246170853
40-41	3.437141892779046E-5	0.0	0.0	0.35615664292976473	0.0011686282435448756
42-43	3.437141892779046E-5	0.0	0.0	0.4749442667442086	0.001202999662472666
44-45	3.437141892779046E-5	0.0	0.0	0.6209712400589263	0.0013232996287199329
46-47	3.437141892779046E-5	0.0	0.0	0.789339635676708	0.001409228176039409
48-49	3.437141892779046E-5	0.0	0.0	0.9612482874440519	0.0014607853044310945
50-51	3.437141892779046E-5	0.0	0.0	1.1436230362749082	0.0015295281422866755
52-53	3.437141892779046E-5	0.0	0.0	1.3299676839919239	0.0017013852369256277
54-55	3.437141892779046E-5	0.0	0.0	1.5417128102965774	0.0018044994937089992
56-57	3.437141892779046E-5	0.0	0.0	1.7847359278255195	0.0018388709126367898
58-59	3.437141892779046E-5	0.0	0.0	2.043054326777329	0.0018560566221006848
60-61	3.437141892779046E-5	0.0	0.0	2.317578849753591	0.0019076137504923706
62-63	3.437141892779046E-5	0.0	0.0	2.605869126010434	0.0019247994599562658
64-65	3.437141892779046E-5	0.0	0.0	2.9071002414935894	0.0019591708788840563
66-67	3.437141892779046E-5	3.437141892779046E-5	0.0	3.2519143161771833	0.0019763565883479514
68-69	3.437141892779046E-5	3.437141892779046E-5	0.0	3.627972010666139	0.001993542297811847
70-71	3.437141892779046E-5	3.437141892779046E-5	0.0	4.016558087354274	0.002096656554595218
72-73	3.437141892779046E-5	3.437141892779046E-5	0.0	4.428207386142956	0.0021310279735230085
74-75	3.437141892779046E-5	3.437141892779046E-5	0.0	4.852419438549746	0.0021825851019146945
76-77	3.437141892779046E-5	3.437141892779046E-5	0.0	5.317670965156317	0.00223414223030638
78-79	3.437141892779046E-5	3.437141892779046E-5	0.0	5.838363590493415	0.00223414223030638
80-81	3.437141892779046E-5	3.437141892779046E-5	0.0	6.380624281207702	0.0022513279397702755
82-83	3.437141892779046E-5	3.437141892779046E-5	0.0	6.943181294798848	0.002302885068161961
84-85	3.437141892779046E-5	3.437141892779046E-5	0.0	7.526550202550771	0.002320070777625856
86-87	3.437141892779046E-5	3.437141892779046E-5	0.0	8.148071385312543	0.0023888136154814367
88-89	3.437141892779046E-5	3.437141892779046E-5	0.0	8.810528928017312	0.0024403707438731227
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2395	0.0	54.101196	1
GTATCAA	4760	0.0	46.96385	1
TCAACGC	5975	0.0	36.54638	4
CAACGCA	6130	0.0	35.61922	5
ATCAACG	6265	0.0	35.08079	3
TATCAAC	6420	0.0	34.528988	2
AACGCAG	6530	0.0	33.563667	6
GTACATG	10155	0.0	30.800318	1
TACATGG	10665	0.0	28.707863	2
ACGCAGA	7680	0.0	27.800919	7
ACATGGG	10985	0.0	26.964602	3
CGCAGAG	7790	0.0	26.49565	8
CATGGGG	8980	0.0	24.951122	4
ATGGGGG	5860	0.0	21.870224	5
GCAGAGT	9190	0.0	21.633224	9
GAGTACA	7295	0.0	20.494389	1
GTATAGG	845	0.0	20.220667	1
AGAGTAC	7835	0.0	18.894789	10-11
TAAGGTG	1445	0.0	18.723803	5
TAAGGTA	795	0.0	18.510536	4
>>END_MODULE