FastQCFastQC Report
Mon 15 Aug 2016
SRR2921711_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921711_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2909394
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24550.054.2163161
GTATCAA47950.042.8270951
TCAACGC60450.032.9730534
CAACGCA61600.032.383095
ATCAACG64300.031.2212663
AACGCAG65050.030.7333436
TATCAAC66800.030.2659442
GTACATG97550.028.5070361
TACATGG104850.025.981522
ACATGGG106150.024.9032653
ACGCAGA80250.024.4979827
CATGGGG86550.023.4135364
CGCAGAG82500.023.3118178
ATGGGGG55050.021.5691185
GTTATAT5550.019.6996421
TAAGGCG8900.019.2115425
GCAGAGT93950.019.1572159
AGAGTAC79900.018.69234510-11
GAGTACA73050.016.9841481
TCTATAC6750.016.8744093
Produced by FastQC (version 0.11.5)