##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921711_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2909394
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.189719921055726	33.0	31.0	34.0	28.0	34.0
2	31.327862778296787	33.0	31.0	34.0	28.0	34.0
3	31.396301085380667	34.0	31.0	34.0	28.0	34.0
4	34.881765412316106	37.0	35.0	37.0	32.0	37.0
5	34.60856487639694	37.0	35.0	37.0	32.0	37.0
6	34.57821560091208	37.0	35.0	37.0	32.0	37.0
7	34.4605639524932	37.0	35.0	37.0	31.0	37.0
8	34.39682696809026	37.0	35.0	37.0	31.0	37.0
9	35.961699584174575	39.0	37.0	39.0	31.0	39.0
10-11	35.84333438509876	39.0	37.0	39.0	30.0	39.0
12-13	35.75880458267254	39.0	37.0	39.0	30.0	39.0
14-15	36.96063028933173	40.0	37.0	41.0	31.0	41.0
16-17	36.91996855702597	40.0	37.0	41.0	31.0	41.0
18-19	36.881929707698575	40.0	37.0	41.0	30.0	41.0
20-21	36.82531087229849	40.0	37.0	41.0	30.0	41.0
22-23	36.73399615177594	40.0	37.0	41.0	30.0	41.0
24-25	36.64087315090359	40.0	37.0	41.0	30.0	41.0
26-27	36.50766087370772	40.0	37.0	41.0	30.0	41.0
28-29	36.380175562333605	40.0	37.0	41.0	29.0	41.0
30-31	36.21215174019057	40.0	36.0	41.0	28.5	41.0
32-33	36.152134602601095	40.0	36.0	41.0	28.5	41.0
34-35	36.277962180440326	40.0	37.0	41.0	29.0	41.0
36-37	36.26404948934383	40.0	36.5	41.0	29.0	41.0
38-39	36.15898912282077	40.0	36.0	41.0	28.5	41.0
40-41	36.07130780499307	40.0	36.0	41.0	28.0	41.0
42-43	35.94172549335016	40.0	36.0	41.0	27.5	41.0
44-45	35.804473027716426	40.0	35.5	41.0	27.0	41.0
46-47	35.64031581834567	40.0	35.0	41.0	26.5	41.0
48-49	35.48751561321705	40.0	35.0	41.0	26.0	41.0
50-51	35.32814032750463	39.0	35.0	41.0	26.0	41.0
52-53	35.15746560967679	39.0	35.0	41.0	25.5	41.0
54-55	34.93886940029436	39.0	35.0	41.0	24.0	41.0
56-57	34.71297338896004	39.0	35.0	41.0	24.0	41.0
58-59	34.47503260129085	38.0	34.0	40.0	23.5	41.0
60-61	34.19188325816304	38.0	34.0	40.0	23.0	41.0
62-63	33.88236313129126	37.0	34.0	40.0	22.0	41.0
64-65	33.548883031999104	37.0	34.0	40.0	22.0	41.0
66-67	33.205026029475555	36.0	33.5	39.0	21.0	41.0
68-69	32.84187995850682	36.0	33.0	39.0	20.0	41.0
70-71	32.44769409024697	35.0	33.0	38.5	18.5	40.0
72-73	32.08638878061892	35.0	33.0	37.5	18.0	39.5
74-75	31.682165426889583	35.0	32.0	37.0	14.0	39.0
76-77	28.989720539741263	33.0	28.0	34.5	7.5	36.5
78-79	30.554775839917177	34.5	31.0	36.0	8.0	37.0
80-81	30.741096771355135	35.0	32.0	35.5	7.0	37.0
82-83	30.55859055184688	35.0	32.0	35.0	6.0	37.0
84-85	30.360422307875798	35.0	32.0	35.0	2.0	36.0
86-87	30.14803168632368	35.0	31.0	35.0	2.0	36.0
88-89	29.880977103822996	34.5	31.0	35.0	2.0	36.0
90-91	29.679131977312114	34.0	31.0	35.0	2.0	35.0
92-93	29.510780595546702	34.0	31.0	35.0	2.0	35.0
94-95	29.395991055181938	34.0	31.0	35.0	2.0	35.0
96-97	29.185185815327866	34.0	31.0	35.0	2.0	35.0
98-99	28.979029309883778	34.0	31.0	35.0	2.0	35.0
100-101	27.843957195209725	33.0	28.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	64848.0
3	21384.0
4	11663.0
5	8083.0
6	6826.0
7	6959.0
8	7141.0
9	7680.0
10	8470.0
11	9183.0
12	9902.0
13	10459.0
14	11043.0
15	12161.0
16	13124.0
17	14007.0
18	12997.0
19	11588.0
20	11235.0
21	11271.0
22	11903.0
23	13232.0
24	15950.0
25	18720.0
26	22474.0
27	26942.0
28	32428.0
29	38805.0
30	47439.0
31	58278.0
32	74645.0
33	97999.0
34	143548.0
35	208785.0
36	359303.0
37	684424.0
38	699747.0
39	84746.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.58631679613997	19.840845263397785	13.506533564946299	26.066304375515948
2	14.990850309412151	21.38755642080053	37.41436343313439	26.207229836652935
3	17.420590342278622	25.637862260801654	29.23242028784239	27.709127109077336
4	13.003246239875265	17.049290323290293	35.65010043956921	34.29736299726524
5	13.929456596516115	37.91004002201709	34.21574758590672	13.944755795560068
6	33.0934960006297	35.96246974822064	17.177579878274503	13.766454372875156
7	28.558603117557514	32.15512651608085	21.39264100793533	17.89362935842631
8	27.05257520982033	34.62153974332799	20.007602957866826	18.318282088984855
9	27.18288660211941	15.16728747199158	19.23274030012446	38.41708562576455
10-11	25.67635924351615	25.47036089342865	27.843975635764796	21.009304227290407
12-13	26.148897714346308	22.854474646115257	27.73026203290625	23.26636560663219
14-15	23.369986939993577	24.36819490568661	24.93407695796548	27.32774119635433
16-17	23.610432275587286	27.332856945466993	25.722796568632507	23.333914210313214
18-19	23.622343329776413	26.829896628166104	26.968638887394665	22.57912115466282
20-21	24.139496123837834	25.97502118574524	26.883976014866366	23.00150667555056
22-23	24.015447890522907	25.668163198246784	26.619443774201773	23.696945137028536
24-25	23.80642054541536	25.931702263610205	26.500242835326716	23.76163435564772
26-27	23.480442459944754	26.15169736078002	26.618472219763657	23.749387959511576
28-29	23.625075470702587	26.41375767532534	26.364982075224763	23.59618477874731
30-31	24.10314252054122	26.039522748578765	26.182686486711333	23.674648244168687
32-33	23.866603186934636	26.16070799380557	26.01866922877282	23.95401959048698
34-35	24.128737566652642	26.25734244676726	26.22516768003432	23.388752306545776
36-37	24.114459797403725	26.07712517532238	26.311598714941326	23.496816312332573
38-39	24.01115146315693	26.229396912209207	26.113376187618453	23.64607543701541
40-41	24.160267914072538	25.97149958529415	26.192773428302633	23.675459072330682
42-43	23.92868240929928	25.970823559499618	26.004063760687053	24.096430270514052
44-45	23.703298856382705	26.00038154950347	26.25168861435657	24.044630979757255
46-47	23.922481390209324	25.95581359497348	26.006324226696663	24.115380788120532
48-49	23.692011753366305	26.151377528080616	26.117965408143483	24.038645310409596
50-51	23.7912990753998	26.070893405535983	26.16950780279114	23.96829971627308
52-53	24.10006004812787	25.87543261634196	26.098868709769558	23.925638625760612
54-55	23.373046590349748	25.83373091275068	26.520655339205135	24.272567157694432
56-57	23.58188689881805	25.896554509627336	26.173678701620567	24.347879889934056
58-59	23.720250853486945	25.825100405679162	26.275383308542022	24.179265432291867
60-61	23.76537374792897	26.00885816621866	26.304577234822187	23.92119085103019
62-63	23.724376354420013	25.905715607336244	26.33639689166892	24.033511146574824
64-65	23.37800817619427	25.671541046537737	26.360231882343676	24.590218894924316
66-67	23.440244957704007	25.90970090837679	26.124035460124556	24.526018673794646
68-69	23.53404717665989	25.79607561553426	26.17980337154964	24.49007383625621
70-71	23.459470187035805	25.640743424201375	26.093366735634728	24.806419653128096
72-73	23.420280327511215	25.94881859374138	25.88899228601651	24.741908792730896
74-75	23.383796649573192	26.057664224667793	25.8915198284269	24.667019297332114
76-77	23.401654895075325	25.58564721878177	26.070789384709798	24.941908501433105
78-79	23.289021779213705	25.54829872252831	26.200261301028693	24.9624181972293
80-81	23.610278465391232	25.829478513269077	25.798127467196625	24.76211555414307
82-83	23.55927538695829	25.78093453085653	25.95852767700911	24.701262405176074
84-85	23.41638892915316	25.79979892509005	25.869351517080446	24.914460628676334
86-87	23.27089192213577	25.985904120988685	25.76094719574279	24.982256761132753
88-89	23.177561491674343	25.675922799546285	25.871221463790288	25.275294244989084
90-91	23.227309988410504	25.556361435167407	26.014960174456082	25.201368401966008
92-93	23.415310600016507	25.337973534347576	26.005856419708934	25.240859445926983
94-95	23.177141922156547	25.317160590945726	26.092030496366014	25.41366699053172
96-97	23.00537558171013	25.13108083138893	26.171563129783127	25.691980457117815
98-99	22.766963402945688	25.126543566853826	26.081302383485657	26.025190646714837
100-101	22.78682520851883	25.20954997603449	26.11274633389582	25.890878481550857
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	81.0
1	68.5
2	80.5
3	115.0
4	112.0
5	127.0
6	209.0
7	395.0
8	571.5
9	607.5
10	516.0
11	423.5
12	432.5
13	479.0
14	513.5
15	541.0
16	599.0
17	678.5
18	750.5
19	848.0
20	978.5
21	1146.0
22	1326.0
23	1565.0
24	1915.0
25	2352.0
26	3073.0
27	4158.5
28	5628.5
29	8128.0
30	11932.0
31	17134.5
32	24738.5
33	31363.0
34	36901.0
35	44032.5
36	53306.0
37	62516.5
38	69414.0
39	77213.0
40	83736.5
41	87935.0
42	92615.5
43	98460.0
44	104309.5
45	110798.5
46	116028.0
47	119644.0
48	123205.5
49	125306.5
50	126084.0
51	125523.5
52	124859.0
53	124871.5
54	123416.5
55	117266.5
56	108209.5
57	97680.5
58	87016.5
59	74579.5
60	60624.0
61	50214.0
62	41807.5
63	31820.0
64	22379.0
65	16238.5
66	12226.0
67	9404.5
68	7182.0
69	5427.5
70	4011.0
71	2846.5
72	1991.5
73	1497.5
74	1130.5
75	789.5
76	536.5
77	322.0
78	177.0
79	102.0
80	61.5
81	43.5
82	30.0
83	18.5
84	10.0
85	5.0
86	5.0
87	4.0
88	1.5
89	2.5
90	3.5
91	2.0
92	1.0
93	1.0
94	0.5
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15632121328359103
2	2.0622851356674277E-4
3	0.01038016851619272
4	0.006255598244857864
5	0.025606707101203892
6	0.002096656554595218
7	3.437141892779046E-5
8	0.0
9	3.437141892779046E-5
10-11	0.04677950116072282
12-13	2.5778564195842843E-4
14-15	0.004485470170076655
16-17	0.0
18-19	0.0014607853044310945
20-21	2.921570608862189E-4
22-23	0.0
24-25	5.155712839168569E-4
26-27	0.0023544421965536466
28-29	0.005241641386488045
30-31	0.016738881017833954
32-33	0.011308196827243062
34-35	0.00969274013763691
36-37	0.003798041791520846
38-39	0.0
40-41	0.003540256149562418
42-43	0.009142797434792262
44-45	0.007011769461269254
46-47	0.0036261846968818935
48-49	0.009280283110503424
50-51	0.002835642061542713
52-53	0.002096656554595218
54-55	0.004193313109190436
56-57	0.007871054934464015
58-59	0.015295281422866755
60-61	0.007355483650547159
62-63	0.021671179633971884
64-65	0.0410738456187096
66-67	0.061524839880744926
68-69	0.050629100080635356
70-71	0.06906936633539493
72-73	0.0334949477451318
74-75	0.026792521054212665
76-77	0.22102540941515655
78-79	0.08256014826455269
80-81	0.06857098076094197
82-83	0.026792521054212665
84-85	0.052330485317560974
86-87	0.06697270978079971
88-89	0.2905244184871489
90-91	0.2918820895347966
92-93	0.05066347149956314
94-95	0.008953754630689415
96-97	0.013971981794146823
98-99	7.046140880197045E-4
100-101	1.5467138517505708E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2909394.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.92771750675222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.1353206278088	43.14002421864847
2	12.255798572544816	13.708776807698872
3	4.174038082454853	7.003332682138824
4	1.920800206443626	4.297038853315619
5	1.1179240489560627	3.126147020200966
6	0.7043633397116866	2.3636060331304654
7	0.4670056228864523	1.8282990985600853
8	0.3664536159716784	1.6395931450713515
9	0.2825538578671392	1.4222333108912744
>10	1.4637966391631623	14.722032031098678
>50	0.08324209140422291	3.1502945066577652
>100	0.02667289803422272	2.519157245300448
>500	0.0012920706612780364	0.5248326714087741
>1k	7.383260920537197E-4	0.5546323758785143
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3748567571116184E-4
2	0.0	0.0	0.0	0.0	1.3748567571116184E-4
3	0.0	0.0	0.0	0.0	1.3748567571116184E-4
4	0.0	0.0	0.0	0.0	1.3748567571116184E-4
5	0.0	0.0	0.0	0.0	1.3748567571116184E-4
6	0.0	0.0	0.0	0.0	1.3748567571116184E-4
7	0.0	0.0	0.0	0.0	1.3748567571116184E-4
8	0.0	0.0	0.0	0.0	1.3748567571116184E-4
9	0.0	0.0	0.0	0.0	1.3748567571116184E-4
10-11	0.0	0.0	0.0	0.0	1.3748567571116184E-4
12-13	0.0	0.0	0.0	0.0	3.0934277035011415E-4
14-15	0.0	0.0	0.0	0.0	4.46828446061276E-4
16-17	0.0	0.0	0.0	5.155712839168569E-5	5.155712839168569E-4
18-19	0.0	0.0	0.0	6.874283785558092E-5	5.499427028446474E-4
20-21	0.0	0.0	0.0	8.592854731947615E-5	6.70242669091914E-4
22-23	0.0	0.0	0.0	1.718570946389523E-4	7.733569258752853E-4
24-25	3.437141892779046E-5	0.0	0.0	4.1245702713348553E-4	8.249140542669711E-4
26-27	3.437141892779046E-5	0.0	0.0	7.217997974835997E-4	8.249140542669711E-4
28-29	3.437141892779046E-5	0.0	0.0	0.0032480990886761987	8.420997637308663E-4
30-31	3.437141892779046E-5	0.0	0.0	0.0158452241257114	8.936568921225519E-4
32-33	3.437141892779046E-5	0.0	0.0	0.04476877315344707	9.623997299781329E-4
34-35	3.437141892779046E-5	0.0	0.0	0.08264607681187217	0.0010139568583698187
36-37	3.437141892779046E-5	0.0	0.0	0.14243516003676368	0.0010311425678337137
38-39	3.437141892779046E-5	0.0	0.0	0.24616810236083525	0.0010655139867615042
40-41	3.437141892779046E-5	0.0	0.0	0.3599374990118217	0.0010655139867615042
42-43	3.437141892779046E-5	0.0	0.0	0.4800656081644493	0.0010655139867615042
44-45	5.155712839168569E-5	0.0	0.0	0.6264019242495172	0.0011342568246170853
46-47	6.874283785558092E-5	0.0	0.0	0.7963170337190495	0.001202999662472666
48-49	6.874283785558092E-5	0.0	0.0	0.9675898142362293	0.0012717425003282471
50-51	6.874283785558092E-5	0.0	0.0	1.1494318060737045	0.0012717425003282471
52-53	6.874283785558092E-5	0.0	0.0	1.3355014824392983	0.0014779710138949898
54-55	6.874283785558092E-5	0.0	0.0	1.5462670233045095	0.0016154566896061516
56-57	6.874283785558092E-5	0.0	0.0	1.789118283738813	0.0016841995274617327
58-59	6.874283785558092E-5	0.0	0.0	2.048278782454353	0.0017529423653173135
60-61	6.874283785558092E-5	0.0	0.0	2.322528334079193	0.001787313784245104
62-63	6.874283785558092E-5	0.0	0.0	2.6124856241540333	0.0018904280410284753
64-65	6.874283785558092E-5	0.0	0.0	2.9154696820025063	0.0019076137504923706
66-67	6.874283785558092E-5	0.0	0.0	3.259785371111647	0.001941985169420161
68-69	1.0311425678337138E-4	0.0	0.0	3.6360492941141693	0.0019591708788840563
70-71	1.0311425678337138E-4	0.0	0.0	4.025271242052469	0.0019763565883479514
72-73	1.0311425678337138E-4	0.0	0.0	4.438192283341479	0.0021825851019146945
74-75	1.0311425678337138E-4	3.437141892779046E-5	0.0	4.8643635066271536	0.0022685136492341705
76-77	1.2029996624726661E-4	3.437141892779046E-5	0.0	5.331694504078856	0.0022685136492341705
78-79	1.3748567571116184E-4	3.437141892779046E-5	0.0	5.856185171207475	0.0022685136492341705
80-81	1.3748567571116184E-4	3.437141892779046E-5	0.0	6.404048403206991	0.0023372564870897516
82-83	1.3748567571116184E-4	3.437141892779046E-5	0.0	6.971125258387142	0.0025434850006564942
84-85	1.3748567571116184E-4	3.437141892779046E-5	0.0	7.55870466495772	0.0025434850006564942
86-87	1.3748567571116184E-4	3.437141892779046E-5	0.0	8.184711317889567	0.0025606707101203897
88-89	1.3748567571116184E-4	3.437141892779046E-5	0.0	8.850520073939796	0.0025778564195842847
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2455	0.0	54.216316	1
GTATCAA	4795	0.0	42.827095	1
TCAACGC	6045	0.0	32.973053	4
CAACGCA	6160	0.0	32.38309	5
ATCAACG	6430	0.0	31.221266	3
AACGCAG	6505	0.0	30.733343	6
TATCAAC	6680	0.0	30.265944	2
GTACATG	9755	0.0	28.507036	1
TACATGG	10485	0.0	25.98152	2
ACATGGG	10615	0.0	24.903265	3
ACGCAGA	8025	0.0	24.497982	7
CATGGGG	8655	0.0	23.413536	4
CGCAGAG	8250	0.0	23.311817	8
ATGGGGG	5505	0.0	21.569118	5
GTTATAT	555	0.0	19.699642	1
TAAGGCG	890	0.0	19.211542	5
GCAGAGT	9395	0.0	19.157215	9
AGAGTAC	7990	0.0	18.692345	10-11
GAGTACA	7305	0.0	16.984148	1
TCTATAC	675	0.0	16.874409	3
>>END_MODULE