FastQCFastQC Report
Mon 15 Aug 2016
SRR2921710_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921710_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3297116
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24450.047.3649251
GTATCAA41750.041.948441
TCAACGC49750.033.5836944
CAACGCA51500.031.9792445
ATCAACG52700.031.6127453
TATCAAC56050.029.9782662
AACGCAG55450.029.6876746
GTACATG75000.027.5912381
TACATGG79900.025.3070622
ACGCAGA63650.025.2698257
ACATGGG83200.024.0743663
CGCAGAG66700.023.8297758
CATGGGG69650.022.1483384
TAAGGCG10800.018.8969045
GCAGAGT81650.018.5952229
AGAGTAC65600.018.00996810-11
CAGAGTA74600.016.5686210-11
ATGGGGG38800.016.5138365
GAGTACA56300.016.1859721
TCTATAC8800.016.180963
Produced by FastQC (version 0.11.5)