##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921710_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3297116
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.301144697365817	31.0	31.0	34.0	26.0	34.0
2	30.35297544884681	31.0	31.0	34.0	26.0	34.0
3	30.41536603504396	33.0	31.0	34.0	26.0	34.0
4	33.85892307095049	37.0	35.0	37.0	30.0	37.0
5	33.854743357528214	37.0	35.0	37.0	30.0	37.0
6	33.89949216224118	37.0	35.0	37.0	30.0	37.0
7	33.80994481237542	37.0	35.0	37.0	29.0	37.0
8	33.78516800743438	37.0	35.0	37.0	29.0	37.0
9	35.339705063455455	39.0	37.0	39.0	29.0	39.0
10-11	35.231801368226044	39.0	35.0	39.0	29.0	39.0
12-13	35.12649281978554	39.0	35.0	39.0	28.0	39.0
14-15	36.263388215640575	40.0	37.0	41.0	27.0	41.0
16-17	36.20503479404425	40.0	37.0	41.0	27.0	41.0
18-19	36.173070647195914	40.0	36.5	41.0	27.0	41.0
20-21	36.098340792377336	40.0	36.5	41.0	27.0	41.0
22-23	35.997691770626204	40.0	36.0	41.0	27.0	41.0
24-25	35.89046412076493	40.0	36.0	41.0	27.0	41.0
26-27	35.74274365839722	40.0	36.0	41.0	27.0	41.0
28-29	35.607921286360565	40.0	36.0	41.0	26.0	41.0
30-31	35.46166407248032	40.0	36.0	41.0	25.0	41.0
32-33	35.28527628387961	39.5	35.0	41.0	25.0	41.0
34-35	35.11266528080905	39.0	35.0	41.0	24.0	41.0
36-37	35.07616095399737	39.0	35.0	41.0	24.0	41.0
38-39	35.24999347914965	39.5	35.0	41.0	24.5	41.0
40-41	35.31307497218781	40.0	35.0	41.0	24.0	41.0
42-43	35.15476449721514	40.0	35.0	41.0	24.0	41.0
44-45	35.03216053059704	40.0	35.0	41.0	23.0	41.0
46-47	34.87718312003581	40.0	35.0	41.0	22.0	41.0
48-49	34.71739423180743	39.0	35.0	41.0	21.0	41.0
50-51	33.036920599699855	37.0	32.5	39.5	17.0	40.0
52-53	33.15304420590601	37.5	33.0	39.5	16.0	40.0
54-55	34.06218525523518	39.0	34.0	40.5	18.0	41.0
56-57	34.08362399139126	39.0	34.0	41.0	17.5	41.0
58-59	33.903690983271446	38.5	34.0	41.0	15.0	41.0
60-61	33.65738587905309	38.0	34.0	40.5	13.0	41.0
62-63	33.36935870621477	37.0	33.5	40.0	11.5	41.0
64-65	33.08654882024169	37.0	33.0	40.0	8.0	41.0
66-67	32.74724698797373	36.0	33.0	39.5	7.0	41.0
68-69	32.39374122718157	36.0	33.0	39.0	3.5	41.0
70-71	31.987366079931675	35.0	32.0	39.0	2.0	40.5
72-73	31.629301183215876	35.0	32.0	37.5	2.0	40.0
74-75	31.232874427226704	35.0	32.0	37.0	2.0	39.0
76-77	30.853072958306594	35.0	32.0	37.0	2.0	39.0
78-79	30.508247662502626	35.0	31.0	36.0	2.0	39.0
80-81	30.19050406476448	35.0	31.0	36.0	2.0	37.0
82-83	29.87185634354387	35.0	31.0	35.0	2.0	37.0
84-85	29.606603164705156	35.0	31.0	35.0	2.0	36.5
86-87	29.367830400871547	35.0	31.0	35.0	2.0	36.0
88-89	29.142276765512648	35.0	30.0	35.0	2.0	36.0
90-91	28.919082313148824	34.0	30.0	35.0	2.0	35.5
92-93	28.72753749034004	34.0	30.0	35.0	2.0	35.0
94-95	28.500762484547103	34.0	30.0	35.0	2.0	35.0
96-97	28.26728829073651	34.0	30.0	35.0	2.0	35.0
98-99	28.003590410528474	34.0	29.0	35.0	2.0	35.0
100-101	26.939380962028636	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	121534.0
3	19949.0
4	13555.0
5	10232.0
6	9317.0
7	9682.0
8	10172.0
9	10769.0
10	11836.0
11	12657.0
12	12453.0
13	12817.0
14	13712.0
15	14691.0
16	14837.0
17	15196.0
18	14369.0
19	13513.0
20	13624.0
21	14550.0
22	16071.0
23	18158.0
24	20968.0
25	24948.0
26	29425.0
27	35011.0
28	41193.0
29	48622.0
30	58505.0
31	70461.0
32	88379.0
33	114067.0
34	156719.0
35	228822.0
36	381921.0
37	692703.0
38	762649.0
39	139007.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.7546505177627	18.178077353055777	12.95071331204261	25.116558817138912
2	15.98646269653372	19.818187336854244	38.918685121107266	25.276664845504765
3	18.841323539142063	23.848088864014237	29.536849926337176	27.77373767050652
4	13.216929752357087	15.989922130520611	37.066218194797536	33.72692992232477
5	14.323300626121055	36.956104756152705	34.60907647903269	14.111518138693551
6	33.25084848277115	35.503024629887406	17.395587859257265	13.85053902808418
7	28.80392200281313	31.57334205320924	21.458933115498212	18.163802828479415
8	26.616086422629277	34.16960307522059	20.4154714755817	18.79883902656843
9	26.77836159273402	15.413038381241511	19.481470000427674	38.327130025596794
10-11	25.378647585576587	25.145745687993358	28.313308562860612	21.16229816356945
12-13	26.095216628538715	22.666249438395177	27.743778965688488	23.494754967377624
14-15	23.2866069843755	24.18536099897293	25.12597150602967	27.4020605106219
16-17	23.6189325951521	27.088175068969374	25.851648689198914	23.44124364667962
18-19	23.56410527460451	26.603687823394335	26.943854262135787	22.888352639865374
20-21	24.18576283608717	25.86947876019778	26.763946682910504	23.18081172080454
22-23	23.92631140280281	25.78093580464882	26.4885942844521	23.804158508096272
24-25	23.796953609351554	25.85861019197407	26.55540136032957	23.789034838344815
26-27	23.673618528645072	26.091642749385624	26.449529809663847	23.78520891230546
28-29	23.808175431965786	26.181177657503753	26.31007807431058	23.70056883621988
30-31	24.043822178475203	25.99158409121759	26.16059992735231	23.803993802954896
32-33	23.763364133051752	26.041517059909687	26.11881841310043	24.07630039393813
34-35	24.17105519575304	26.06583817303733	26.125984717682037	23.63712191352759
36-37	24.22070863529079	25.83403647692607	26.13457349463073	23.810681393152404
38-39	23.926686373554514	26.027059259578294	26.17898589802929	23.867268468837903
40-41	24.2337613888653	25.824729760879322	26.214635374331223	23.72687347592415
42-43	24.02732214938417	25.832347767509113	26.03444132667369	24.105888756433032
44-45	24.012622171491227	25.849878394522026	26.159187542299044	23.978311891687703
46-47	24.12361073395735	25.875393934678602	25.964137983014936	24.036857348349113
48-49	23.93281150791902	25.913029840580137	26.03314714947827	24.121011502022572
50-51	23.98642786005543	26.002175408162554	26.089474373612827	23.921922358169194
52-53	24.205334757377987	25.832702918004873	26.050929453561363	23.911032871055777
54-55	23.79130285727832	25.74749285703214	26.27381280546236	24.187391480227177
56-57	23.823341152499268	25.911249933894716	26.185016986778336	24.080391926827684
58-59	23.887993896489572	25.668772094069443	26.29030885343368	24.152925156007306
60-61	23.819046841745855	25.73881718424868	26.392297816700477	24.04983815730499
62-63	23.861318386507495	25.76247062715609	26.334267560603074	24.04194342573334
64-65	23.718609077543203	25.685974903218206	26.226813210192194	24.36860280904639
66-67	23.71148668383013	25.720104074888695	26.206424110945946	24.361985130335228
68-69	23.86917607329466	25.60263656059461	26.254832473737267	24.273354892373465
70-71	23.7835232683861	25.44357345718667	26.228495180769023	24.54440809365821
72-73	23.794279451848297	25.578487989103948	26.167236993204252	24.4599955658435
74-75	23.808816850030034	25.68467416956916	26.18107044535865	24.325438535042153
76-77	23.86138392711301	25.454878908390903	26.243254138229215	24.440483026266865
78-79	23.808045310417945	25.496821292054506	26.231149027846673	24.463984369680876
80-81	23.950465478587983	25.526161796557357	26.152676976859063	24.37069574799559
82-83	23.919847681534396	25.5379197987407	26.220431642954427	24.321800876770478
84-85	23.895125257360704	25.54811541370804	26.213837468253082	24.34292186067817
86-87	23.865553959599183	25.584231255142754	26.168762733849455	24.381452051408612
88-89	23.860720214246662	25.430041439686736	26.201318476068934	24.507919869997668
90-91	23.807520892677523	25.4730370946208	26.238789049359422	24.480652963342255
92-93	23.92131248611065	25.287842035197393	26.35586648105987	24.434978997632083
94-95	23.87464097304191	25.189399783690767	26.439417834794774	24.49654140847254
96-97	23.909736396968672	25.044211020458608	26.432633927885984	24.613418654686733
98-99	23.86864592604303	25.007953874369065	26.356076676412982	24.767323523174923
100-101	24.004874363968014	24.89932866520961	26.44170670235687	24.654090268465506
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	24.5
2	60.0
3	93.5
4	80.5
5	90.5
6	140.0
7	280.0
8	451.0
9	535.0
10	505.5
11	468.5
12	514.0
13	603.5
14	707.5
15	802.0
16	911.0
17	1001.5
18	1114.0
19	1226.5
20	1373.0
21	1540.0
22	1691.5
23	1916.5
24	2245.5
25	2701.0
26	3457.0
27	4560.0
28	6285.0
29	8974.5
30	13094.5
31	19191.5
32	27121.0
33	33937.5
34	40160.5
35	48332.0
36	57971.0
37	67781.5
38	76930.0
39	85681.0
40	93419.5
41	100983.5
42	108674.5
43	115703.5
44	122701.5
45	129896.0
46	134997.0
47	138695.5
48	142146.5
49	143485.5
50	143017.0
51	142407.0
52	140805.5
53	138520.0
54	135006.0
55	128110.0
56	118284.0
57	106113.0
58	95484.0
59	83426.0
60	68696.0
61	57155.0
62	47270.5
63	36509.5
64	26665.5
65	19695.5
66	14936.5
67	11688.5
68	9073.0
69	6961.0
70	5386.5
71	4019.0
72	2999.5
73	2291.0
74	1793.5
75	1409.5
76	1094.0
77	769.5
78	508.0
79	359.5
80	272.5
81	210.0
82	167.5
83	126.5
84	95.5
85	80.0
86	71.0
87	62.5
88	41.0
89	31.0
90	35.5
91	33.5
92	35.5
93	32.5
94	21.0
95	19.0
96	18.0
97	13.5
98	8.5
99	6.5
100	8.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05410789307989164
2	0.07630911378307588
3	0.07200231960294996
4	0.07194166052999046
5	0.0675135482039455
6	0.0105850082314362
7	0.01313268929573603
8	0.017348494866422655
9	0.006642168489067415
10-11	0.01361796187941219
12-13	0.023020118188137755
14-15	0.04036861305456041
16-17	0.027493724818902335
18-19	0.010539513926716561
20-21	0.02083639156159504
22-23	0.037805767222020704
24-25	0.03487896695172387
26-27	0.020775732488635523
28-29	0.011676871544707557
30-31	0.011631377239987917
32-33	0.008446775909613128
34-35	0.005110526896839541
36-37	5.45931656635678E-4
38-39	0.0039428397423687854
40-41	0.001091863313271356
42-43	0.22900316519042702
44-45	0.19887077069778558
46-47	0.20486085415253819
48-49	0.21227642582183948
50-51	0.2031775648779115
52-53	0.21100258528968951
54-55	0.20675645018252314
56-57	0.20957709707514083
58-59	0.21855463987314974
60-61	0.2228462692850358
62-63	0.20850039853010935
64-65	0.20786347826403437
66-67	0.20602854130700893
68-69	0.20069054288657118
70-71	0.1923499203546372
72-73	0.20470920647013935
74-75	0.20322305918263114
76-77	0.1967628679124423
78-79	0.2153397090062952
80-81	0.2271985577698813
82-83	0.22533329127637608
84-85	0.21926738398042409
86-87	0.23961850295834297
88-89	0.2316115053276864
90-91	0.2385569691815514
92-93	0.23429566930614512
94-95	0.23788971937899667
96-97	0.24447122879510458
98-99	0.23773807169659789
100-101	0.24480485369638194
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3297116.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.28881815618497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.7181220957863	52.69990795420527
2	11.202126377623662	14.627471840625486
3	3.5376260168514015	6.9290226515640025
4	1.513700826845503	3.953109521071315
5	0.8381420940086614	2.736065338238779
6	0.5060229764582296	1.9822585255699687
7	0.33812950449431184	1.545325301251904
8	0.239065034866407	1.2486618871115893
9	0.1871027002098184	1.0994142753477023
>10	0.8735512906355032	9.692212547514579
>50	0.035258321884386955	1.5283822836662933
>100	0.009663859724316213	1.0608113451553003
>500	0.0010236191734564561	0.4763827057784513
>1k	4.652814380209536E-4	0.4209738228994035
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.098860943927966E-5
2	0.0	0.0	0.0	0.0	9.098860943927966E-5
3	0.0	0.0	0.0	0.0	9.098860943927966E-5
4	0.0	0.0	0.0	0.0	9.098860943927966E-5
5	0.0	0.0	0.0	0.0	9.098860943927966E-5
6	0.0	0.0	0.0	0.0	9.098860943927966E-5
7	0.0	0.0	0.0	0.0	9.098860943927966E-5
8	0.0	0.0	0.0	0.0	9.098860943927966E-5
9	3.0329536479759888E-5	0.0	0.0	3.0329536479759888E-5	1.5164768239879945E-4
10-11	3.0329536479759888E-5	0.0	0.0	6.0659072959519777E-5	1.5164768239879945E-4
12-13	3.0329536479759888E-5	0.0	0.0	6.0659072959519777E-5	1.5164768239879945E-4
14-15	3.0329536479759888E-5	0.0	0.0	6.0659072959519777E-5	2.2747152359819917E-4
16-17	3.0329536479759888E-5	0.0	0.0	1.0615337767915962E-4	3.6395443775711863E-4
18-19	3.0329536479759888E-5	0.0	0.0	1.5164768239879945E-4	3.6395443775711863E-4
20-21	3.0329536479759888E-5	0.0	0.0	1.5164768239879945E-4	3.7911920599699857E-4
22-23	3.0329536479759888E-5	0.0	0.0	1.8197721887855932E-4	4.246135107166384E-4
24-25	3.0329536479759888E-5	0.0	0.0	3.1846013303747883E-4	4.852725836761582E-4
26-27	3.0329536479759888E-5	0.0	0.0	6.217554978350777E-4	5.156021201559181E-4
28-29	3.0329536479759888E-5	0.0	0.0	0.002320209540701631	5.30766888395798E-4
30-31	3.0329536479759888E-5	0.0	0.0	0.011813354458866476	5.45931656635678E-4
32-33	3.0329536479759888E-5	0.0	0.0	0.03440885913628759	6.217554978350777E-4
34-35	3.0329536479759888E-5	0.0	0.0	0.06705860515674911	6.369202660749577E-4
36-37	3.0329536479759888E-5	0.0	0.0	0.11867947624530044	6.369202660749577E-4
38-39	3.0329536479759888E-5	0.0	0.0	0.201721747126883	6.369202660749577E-4
40-41	3.0329536479759888E-5	0.0	0.0	0.29868527525267535	6.369202660749577E-4
42-43	3.0329536479759888E-5	0.0	0.0	0.4000465861680329	6.672498025547175E-4
44-45	3.0329536479759888E-5	0.0	0.0	0.5268240486534292	7.430736437541172E-4
46-47	3.0329536479759888E-5	0.0	0.0	0.6761060272068073	8.03732716713637E-4
48-49	3.0329536479759888E-5	0.0	0.0	0.8318027027256548	8.795565579130367E-4
50-51	4.549430471963983E-5	0.0	0.0	0.9970835117721063	9.402156308725564E-4
52-53	6.0659072959519777E-5	0.0	0.0	1.164077939629664	0.0010160394720719562
54-55	6.0659072959519777E-5	0.0	0.0	1.3564885190572609	0.001046369008551716
56-57	6.0659072959519777E-5	0.0	0.0	1.57766666383591	0.001076698545031476
58-59	6.0659072959519777E-5	1.5164768239879944E-5	0.0	1.812204969433893	0.001091863313271356
60-61	6.0659072959519777E-5	3.0329536479759888E-5	0.0	2.051975120074635	0.001091863313271356
62-63	6.0659072959519777E-5	3.0329536479759888E-5	0.0	2.3044685112686363	0.0011221928497511158
64-65	6.0659072959519777E-5	3.0329536479759888E-5	0.0	2.580831247672208	0.0011221928497511158
66-67	6.0659072959519777E-5	3.0329536479759888E-5	0.0	2.9020665332975852	0.0011676871544707558
68-69	7.582384119939972E-5	3.0329536479759888E-5	0.0	3.2484450046646827	0.0012131814591903956
70-71	9.098860943927966E-5	6.0659072959519777E-5	0.0	3.609063193409028	0.0012435109956701555
72-73	9.098860943927966E-5	6.0659072959519777E-5	0.0	3.9807061686637653	0.0013041700686296752
74-75	9.098860943927966E-5	6.0659072959519777E-5	0.0	4.373898279587372	0.001334499605109435
76-77	9.098860943927966E-5	6.0659072959519777E-5	0.0	4.813054803046056	0.001334499605109435
78-79	9.098860943927966E-5	6.0659072959519777E-5	0.0	5.298388045795174	0.001334499605109435
80-81	9.098860943927966E-5	6.0659072959519777E-5	0.0	5.803526475865574	0.001364829141589195
82-83	9.098860943927966E-5	6.0659072959519777E-5	0.0	6.322980447154423	0.0015468063604677543
84-85	9.098860943927966E-5	6.0659072959519777E-5	0.0	6.864650803914694	0.0015468063604677543
86-87	9.098860943927966E-5	6.0659072959519777E-5	0.0	7.449161630952627	0.0015468063604677543
88-89	9.098860943927966E-5	6.0659072959519777E-5	0.0	8.077604791581491	0.0015468063604677543
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2445	0.0	47.364925	1
GTATCAA	4175	0.0	41.94844	1
TCAACGC	4975	0.0	33.583694	4
CAACGCA	5150	0.0	31.979244	5
ATCAACG	5270	0.0	31.612745	3
TATCAAC	5605	0.0	29.978266	2
AACGCAG	5545	0.0	29.687674	6
GTACATG	7500	0.0	27.591238	1
TACATGG	7990	0.0	25.307062	2
ACGCAGA	6365	0.0	25.269825	7
ACATGGG	8320	0.0	24.074366	3
CGCAGAG	6670	0.0	23.829775	8
CATGGGG	6965	0.0	22.148338	4
TAAGGCG	1080	0.0	18.896904	5
GCAGAGT	8165	0.0	18.595222	9
AGAGTAC	6560	0.0	18.009968	10-11
CAGAGTA	7460	0.0	16.56862	10-11
ATGGGGG	3880	0.0	16.513836	5
GAGTACA	5630	0.0	16.185972	1
TCTATAC	880	0.0	16.18096	3
>>END_MODULE