FastQCFastQC Report
Mon 15 Aug 2016
SRR2921710_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921710_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3297116
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40750.045.1401671
GGTATCA24400.041.1028981
TCAACGC50150.035.772394
CAACGCA51500.034.953335
ATCAACG53200.033.899953
TATCAAC55300.033.0422252
AACGCAG56250.032.082266
ACGCAGA67700.026.234557
CGCAGAG69000.025.1896828
GTACATG78850.024.3533921
TACATGG82150.023.1670362
ACATGGG85200.021.6689113
GCAGAGT86800.019.422249
GTATAGC11600.019.2583771
CATGGGG72400.019.0101934
TAAGGTG14250.018.3317745
AGAGTAC70850.018.03030810-11
GTAAGGT13850.016.1055364
TCTATAC8300.016.0105783
ATTATAC10900.015.6748383
Produced by FastQC (version 0.11.5)