##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921710_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3297116
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.254480582424154	33.0	31.0	34.0	28.0	34.0
2	31.39455148074863	34.0	31.0	34.0	28.0	34.0
3	31.46661688578746	34.0	31.0	34.0	28.0	34.0
4	34.93799854175589	37.0	35.0	37.0	32.0	37.0
5	34.6685664077333	37.0	35.0	37.0	32.0	37.0
6	34.639726961380795	37.0	35.0	37.0	32.0	37.0
7	34.52250117981897	37.0	35.0	37.0	31.0	37.0
8	34.46381201025381	37.0	35.0	37.0	31.0	37.0
9	36.0439808608493	39.0	37.0	39.0	31.0	39.0
10-11	35.93135349196085	39.0	37.0	39.0	31.0	39.0
12-13	35.85568387645445	39.0	37.0	39.0	30.0	39.0
14-15	37.08512242214105	40.0	38.0	41.0	31.0	41.0
16-17	37.051527152820825	40.0	38.0	41.0	31.0	41.0
18-19	37.00883453903351	40.0	38.0	41.0	31.0	41.0
20-21	36.95192268030606	40.0	38.0	41.0	30.5	41.0
22-23	36.859181175305935	40.0	37.0	41.0	30.0	41.0
24-25	36.772383046274385	40.0	37.0	41.0	30.0	41.0
26-27	36.64413778587105	40.0	37.0	41.0	30.0	41.0
28-29	36.520899173702105	40.0	37.0	41.0	30.0	41.0
30-31	36.36134670421059	40.0	37.0	41.0	29.5	41.0
32-33	36.309650464223886	40.0	36.5	41.0	29.5	41.0
34-35	36.4371368796245	40.0	37.0	41.0	30.0	41.0
36-37	36.42812915893769	40.0	37.0	41.0	30.0	41.0
38-39	36.31989138386396	40.0	37.0	41.0	29.0	41.0
40-41	36.237354099764765	40.0	37.0	41.0	29.0	41.0
42-43	36.111537476995046	40.0	36.0	41.0	28.0	41.0
44-45	35.976781526643286	40.0	36.0	41.0	28.0	41.0
46-47	35.822844115887946	40.0	36.0	41.0	27.0	41.0
48-49	35.67732466798256	40.0	35.5	41.0	27.0	41.0
50-51	35.520660935193064	39.5	35.0	41.0	26.5	41.0
52-53	35.346052428849944	39.0	35.0	41.0	26.0	41.0
54-55	35.135914386997605	39.0	35.0	41.0	26.0	41.0
56-57	34.911731646687585	39.0	35.0	41.0	25.5	41.0
58-59	34.67623401785075	38.5	35.0	40.5	25.0	41.0
60-61	34.39997136891756	38.0	34.0	40.0	24.0	41.0
62-63	34.09958384842996	37.5	34.0	40.0	23.0	41.0
64-65	33.77078998737078	37.0	34.0	40.0	22.5	41.0
66-67	33.422003654102554	36.5	34.0	39.0	22.0	41.0
68-69	33.06423204400452	36.0	33.0	39.0	21.5	41.0
70-71	32.667912351279114	35.5	33.0	39.0	20.0	40.0
72-73	32.30515653680368	35.0	33.0	37.5	19.5	40.0
74-75	31.899026603856218	35.0	32.5	37.0	18.0	39.0
76-77	29.19763969481207	33.0	29.0	34.5	10.5	36.5
78-79	30.755958237441448	34.5	31.5	36.0	11.5	37.5
80-81	30.93394848710206	35.0	32.0	36.0	11.5	37.0
82-83	30.751701032053468	35.0	32.0	35.0	9.0	37.0
84-85	30.543829516462267	35.0	32.0	35.0	7.0	36.5
86-87	30.328054578607485	35.0	32.0	35.0	2.0	36.0
88-89	30.051924621396395	35.0	31.0	35.0	2.0	36.0
90-91	29.849716085209014	34.0	31.0	35.0	2.0	35.0
92-93	29.688019924079104	34.0	31.0	35.0	2.0	35.0
94-95	29.57718351431979	34.0	31.0	35.0	2.0	35.0
96-97	29.37177384720465	34.0	31.0	35.0	2.0	35.0
98-99	29.159595537433322	34.0	31.0	35.0	2.0	35.0
100-101	28.039993436688306	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	71652.0
3	23168.0
4	12313.0
5	8651.0
6	7639.0
7	7399.0
8	7783.0
9	8274.0
10	9265.0
11	10074.0
12	10647.0
13	11505.0
14	12066.0
15	13208.0
16	14415.0
17	14832.0
18	14169.0
19	12686.0
20	12296.0
21	12222.0
22	12843.0
23	14565.0
24	17194.0
25	20172.0
26	24313.0
27	29538.0
28	35808.0
29	42357.0
30	52399.0
31	64833.0
32	82183.0
33	107793.0
34	152967.0
35	229367.0
36	402636.0
37	778146.0
38	831615.0
39	104120.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.523619218611636	19.77625577018597	13.53301708385168	26.16710792735072
2	15.04298307124538	21.257997908472625	37.27007150500196	26.428947515280036
3	17.481999069393293	25.01229990287475	29.31307999881096	28.192621028921
4	12.865145160253183	17.32829669496389	35.91950397160006	33.887054173182875
5	14.077211163476457	37.25927824808838	34.64136141140708	14.022149177028078
6	33.19520990121172	36.138082183138806	17.070497241322624	13.596210674326844
7	28.506124746596722	31.81889869813498	21.51358945211512	18.161387103153178
8	26.598184595264467	34.56878678214537	20.136234211959785	18.696794410630382
9	26.570220762630132	15.351567855058784	19.396072203707725	38.68213917860336
10-11	25.301644999812623	25.357570229328335	28.26000771058102	21.080777060278024
12-13	25.992745457266846	22.809302641573755	27.780115686284322	23.417836214875074
14-15	23.12190291170848	24.362823490516607	25.062046028940404	27.453227568834503
16-17	23.50182705127754	27.333205747083205	25.76037664431582	23.40459055732343
18-19	23.43400162447461	26.7987962631018	26.91412607451595	22.853076037907645
20-21	24.08970534913123	25.986717743790727	26.74077308111615	23.18280382596189
22-23	23.835891730833854	25.86787968636833	26.504208526481932	23.79202005631588
24-25	23.718521773904072	25.98709077870086	26.53028016586088	23.764107281534184
26-27	23.557771560387252	26.2642573629647	26.462906174946532	23.715064901701517
28-29	23.704103401476917	26.330603070639384	26.352896500401663	23.612397027482036
30-31	23.92084904225206	26.119928294982515	26.177488270733008	23.781734392032426
32-33	23.7402774516959	26.160396491298314	26.078117691595093	24.02120836541069
34-35	24.06364605771691	26.180676897205394	26.135481055890118	23.62019598918758
36-37	24.095663997656033	25.96680231618237	26.196285263743118	23.741248422418476
38-39	23.86417402360123	26.134627959707817	26.201959530692886	23.799238485998067
40-41	24.064646599449045	25.983298465803408	26.189500917879194	23.762554016868354
42-43	23.946564459456837	25.96333389144488	26.04559467998369	24.044506969114593
44-45	23.93399343137338	25.952399295819934	26.145278390140508	23.968328882666178
46-47	24.039429201958875	26.015560215687117	25.94062841409064	24.004382168263373
48-49	23.844119839451714	26.063435226744495	25.991123284430255	24.101321649373528
50-51	23.896073644528844	26.135151456145188	26.016453611086593	23.952321288239375
52-53	24.11310841907415	25.94947785125426	25.94457953157505	23.992834198096542
54-55	23.700922373436203	25.880384296736697	26.15571172861496	24.26298160121214
56-57	23.738294568752696	26.02332760162906	26.064214904635218	24.174162924983023
58-59	23.7215225928688	25.86221096020432	26.140283104561586	24.275983342365294
60-61	23.74943526160563	25.968548147256172	26.210155327134245	24.071861264003957
62-63	23.75280245464767	25.930970087280507	26.13740487656307	24.17882258150875
64-65	23.545208252611445	25.817719684651376	26.093648212669468	24.543423850067708
66-67	23.55607416719712	25.908581547423502	26.004996779330664	24.530347506048713
68-69	23.6737702972977	25.79900292858246	26.045585262836557	24.481641511283282
70-71	23.58677679428829	25.65267126455759	26.036725866232207	24.72382607492191
72-73	23.575290821679328	25.799240049927093	25.924861787266163	24.700607341127412
74-75	23.571378839009572	25.889464313803895	25.848659893335117	24.690496953851415
76-77	23.602571606573193	25.66785456825854	25.965754570827173	24.763819254341094
78-79	23.53902904275121	25.7523109501335	25.952458719807087	24.7562012873082
80-81	23.618016560128343	25.81636722427556	25.831193158400463	24.73442305719563
82-83	23.589221934383488	25.820358089722962	25.87804550252062	24.71237447337293
84-85	23.535300523166832	25.812618545513967	25.85429771061975	24.797783220699447
86-87	23.508024896032584	25.86838305534079	25.763296486011345	24.86029556261528
88-89	23.38977668290871	25.758341063475992	25.783375622879152	25.06850663073614
90-91	23.294857288700534	25.7850440949191	25.84342056290551	25.076678053474854
92-93	23.386832842318896	25.632744060374062	25.936850844748655	25.043572252558388
94-95	23.23746628536624	25.595306704586402	25.955354120434798	25.211872889612557
96-97	23.195679820786275	25.483612394200428	25.99947370376802	25.321234081245276
98-99	23.07705139539927	25.48907595648884	25.876160305239328	25.55771234287257
100-101	23.01184507576866	25.50025272143759	25.88763760731562	25.60026459547813
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	23.0
2	44.5
3	74.5
4	70.0
5	72.0
6	123.5
7	236.0
8	350.5
9	369.0
10	316.0
11	288.5
12	298.5
13	347.0
14	391.5
15	405.5
16	460.5
17	542.0
18	613.0
19	722.5
20	859.0
21	1019.5
22	1232.5
23	1540.5
24	1934.5
25	2469.5
26	3328.5
27	4635.5
28	6665.5
29	9616.5
30	13941.0
31	19994.0
32	28158.5
33	35206.5
34	40922.5
35	48528.5
36	58286.0
37	68089.5
38	76995.0
39	85393.5
40	93177.5
41	101195.0
42	108848.0
43	115629.0
44	122292.0
45	129996.5
46	135306.0
47	139145.0
48	143598.0
49	145357.5
50	144520.5
51	142890.5
52	141372.5
53	139986.0
54	136579.5
55	129028.0
56	118951.5
57	106620.5
58	95161.5
59	83053.0
60	68398.0
61	56407.5
62	46547.0
63	35710.5
64	25654.5
65	18722.5
66	14127.5
67	11148.0
68	8535.0
69	6358.5
70	4855.5
71	3613.5
72	2635.5
73	2040.0
74	1590.5
75	1194.0
76	874.5
77	568.0
78	324.0
79	189.5
80	122.5
81	75.5
82	41.5
83	20.5
84	12.5
85	15.5
86	12.5
87	6.0
88	5.5
89	5.5
90	4.0
91	2.0
92	2.0
93	2.5
94	2.0
95	2.0
96	1.0
97	0.5
98	1.0
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16411312189198074
2	1.2131814591903955E-4
3	0.010372701476077882
4	0.006308543587790057
5	0.025203844814680465
6	0.0019714198711843927
7	0.0
8	0.0
9	0.0
10-11	0.049876922740965134
12-13	2.881305965577189E-4
14-15	0.005110526896839541
16-17	0.0
18-19	0.0015164768239879945
20-21	3.1846013303747883E-4
22-23	0.0
24-25	4.701078154362782E-4
26-27	0.0027751525878980297
28-29	0.005474481334596659
30-31	0.01681772797802686
32-33	0.012071155518944434
34-35	0.01069116160911536
36-37	0.004215805570686624
38-39	0.0
40-41	0.0034424023904527476
42-43	0.00861358836025181
44-45	0.007036452463304294
46-47	0.0035788853046116666
48-49	0.008750071274410728
50-51	0.00269932874669863
52-53	0.0020320789441439125
54-55	0.0040186635835681855
56-57	0.007552054583460212
58-59	0.014846308106842464
60-61	0.007142605840983454
62-63	0.0204876018920778
64-65	0.04048993120047945
66-67	0.06046193097240134
68-69	0.050498678238800214
70-71	0.06890870688201448
72-73	0.033665785492533476
74-75	0.02743306574594282
76-77	0.225090654984538
78-79	0.08492270214332769
80-81	0.0671495937661884
82-83	0.027387571441223175
84-85	0.05169669492975072
86-87	0.06713442899794851
88-89	0.2929984871627204
90-91	0.2982151674372391
92-93	0.05239427426878521
94-95	0.009948087965361244
96-97	0.014967626252761506
98-99	9.402156308725564E-4
100-101	2.2747152359819915E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3297116.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.56531699265493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.05527772125872	51.687943830080954
2	11.616677671813763	15.000689525643043
3	3.6202666316473446	7.012309880107257
4	1.5723574062025971	4.060790174288774
5	0.8747670738238829	2.823980670808808
6	0.5294866633558168	2.0511884557770887
7	0.35661600840888463	1.611751793930245
8	0.24596866392895753	1.270483580546636
9	0.18280913511441182	1.0622816782033575
>10	0.8998246724125035	9.982083643603573
>50	0.03490501789609826	1.5009738184536738
>100	0.009537055477150272	1.0407616534949216
>500	0.0011767802037224843	0.5782016117262478
>1k	3.2949845610732775E-4	0.31655968333534096
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.0329536479759888E-5	0.0
5	0.0	0.0	0.0	3.0329536479759888E-5	0.0
6	0.0	0.0	0.0	3.0329536479759888E-5	3.0329536479759888E-5
7	0.0	0.0	0.0	3.0329536479759888E-5	3.0329536479759888E-5
8	0.0	0.0	0.0	3.0329536479759888E-5	3.0329536479759888E-5
9	0.0	0.0	0.0	3.0329536479759888E-5	3.0329536479759888E-5
10-11	0.0	0.0	0.0	6.0659072959519777E-5	3.0329536479759888E-5
12-13	0.0	0.0	0.0	6.0659072959519777E-5	7.582384119939972E-5
14-15	0.0	0.0	0.0	6.0659072959519777E-5	1.364829141589195E-4
16-17	0.0	0.0	0.0	7.582384119939972E-5	1.8197721887855932E-4
18-19	0.0	3.0329536479759888E-5	0.0	9.098860943927966E-5	1.8197721887855932E-4
20-21	0.0	3.0329536479759888E-5	0.0	9.098860943927966E-5	2.2747152359819917E-4
22-23	0.0	3.0329536479759888E-5	0.0	1.2131814591903955E-4	2.426362918380791E-4
24-25	0.0	3.0329536479759888E-5	0.0	2.426362918380791E-4	3.1846013303747883E-4
26-27	0.0	3.0329536479759888E-5	0.0	5.914259613553178E-4	3.3362490127735876E-4
28-29	0.0	3.0329536479759888E-5	0.0	0.0023657038454212714	3.3362490127735876E-4
30-31	1.5164768239879944E-5	3.0329536479759888E-5	0.0	0.011965002141265276	3.487896695172387E-4
32-33	3.0329536479759888E-5	3.0329536479759888E-5	0.0	0.035000285097642914	3.6395443775711863E-4
34-35	3.0329536479759888E-5	3.0329536479759888E-5	0.0	0.06818079800650023	4.246135107166384E-4
36-37	3.0329536479759888E-5	3.0329536479759888E-5	0.0	0.1203021064469676	4.246135107166384E-4
38-39	3.0329536479759888E-5	3.0329536479759888E-5	0.0	0.20355668408390848	4.549430471963983E-4
40-41	3.0329536479759888E-5	3.0329536479759888E-5	0.0	0.30062636558738	4.549430471963983E-4
42-43	3.0329536479759888E-5	3.0329536479759888E-5	0.0	0.40326151703488744	4.549430471963983E-4
44-45	3.0329536479759888E-5	3.0329536479759888E-5	0.0	0.5304635930310004	5.156021201559181E-4
46-47	9.098860943927966E-5	3.0329536479759888E-5	0.0	0.6809132587388493	6.217554978350777E-4
48-49	9.098860943927966E-5	3.0329536479759888E-5	0.0	0.8362763093564194	6.520850343148376E-4
50-51	9.098860943927966E-5	3.0329536479759888E-5	0.0	1.0009353629050357	6.975793390344774E-4
52-53	9.098860943927966E-5	3.0329536479759888E-5	0.0	1.1677174840072353	7.279088755142373E-4
54-55	9.098860943927966E-5	3.0329536479759888E-5	0.0	1.3592788364133987	7.885679484737571E-4
56-57	9.098860943927966E-5	3.0329536479759888E-5	0.0	1.580335663046129	8.03732716713637E-4
58-59	9.098860943927966E-5	3.0329536479759888E-5	0.0	1.8145403437428347	8.18897484953517E-4
60-61	9.098860943927966E-5	3.0329536479759888E-5	0.0	2.0550838975638106	8.18897484953517E-4
62-63	9.098860943927966E-5	3.0329536479759888E-5	0.0	2.3074711353801325	8.18897484953517E-4
64-65	9.098860943927966E-5	3.0329536479759888E-5	0.0	2.5842129909897014	8.18897484953517E-4
66-67	9.098860943927966E-5	3.0329536479759888E-5	0.0	2.9069344239025865	8.18897484953517E-4
68-69	9.098860943927966E-5	3.0329536479759888E-5	0.0	3.2523726796388113	8.18897484953517E-4
70-71	9.098860943927966E-5	3.0329536479759888E-5	0.0	3.6139310840140295	8.18897484953517E-4
72-73	9.098860943927966E-5	3.0329536479759888E-5	0.0	3.9871815247021942	8.947213261529166E-4
74-75	9.098860943927966E-5	3.0329536479759888E-5	0.0	4.381480663707313	9.250508626326765E-4
76-77	9.098860943927966E-5	3.0329536479759888E-5	0.0	4.8220778401487845	9.402156308725564E-4
78-79	9.098860943927966E-5	3.0329536479759888E-5	0.0	5.3081238270051765	9.402156308725564E-4
80-81	9.098860943927966E-5	3.0329536479759888E-5	0.0	5.817174767281466	9.705451673523164E-4
82-83	9.098860943927966E-5	3.0329536479759888E-5	0.0	6.340283447716125	0.0011676871544707556
84-85	9.098860943927966E-5	3.0329536479759888E-5	0.0	6.8865942235578	0.0011828519227106355
86-87	9.098860943927966E-5	3.0329536479759888E-5	0.0	7.476412719479691	0.0011980166909505157
88-89	9.098860943927966E-5	3.0329536479759888E-5	0.0	8.110330361443152	0.0012131814591903956
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4075	0.0	45.140167	1
GGTATCA	2440	0.0	41.102898	1
TCAACGC	5015	0.0	35.77239	4
CAACGCA	5150	0.0	34.95333	5
ATCAACG	5320	0.0	33.89995	3
TATCAAC	5530	0.0	33.042225	2
AACGCAG	5625	0.0	32.08226	6
ACGCAGA	6770	0.0	26.23455	7
CGCAGAG	6900	0.0	25.189682	8
GTACATG	7885	0.0	24.353392	1
TACATGG	8215	0.0	23.167036	2
ACATGGG	8520	0.0	21.668911	3
GCAGAGT	8680	0.0	19.42224	9
GTATAGC	1160	0.0	19.258377	1
CATGGGG	7240	0.0	19.010193	4
TAAGGTG	1425	0.0	18.331774	5
AGAGTAC	7085	0.0	18.030308	10-11
GTAAGGT	1385	0.0	16.105536	4
TCTATAC	830	0.0	16.010578	3
ATTATAC	1090	0.0	15.674838	3
>>END_MODULE