FastQCFastQC Report
Mon 15 Aug 2016
SRR2921709_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921709_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3496282
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24350.045.2213131
GTATCAA44400.044.790431
TCAACGC55400.034.957624
CAACGCA56850.034.0625845
GTACATG108100.032.4468771
ATCAACG59950.032.144263
TATCAAC61800.031.5664792
AACGCAG61950.031.3175356
TACATGG115100.029.8563042
ACATGGG118650.028.4025423
CATGGGG100650.025.8439084
ACGCAGA74350.025.2693657
CGCAGAG79050.023.7082868
ATGGGGG61450.022.2442915
GAGTACA79450.020.849021
GTATAGG9400.019.6920491
GCAGAGT91100.019.6880749
AGAGTAC76450.019.11626610-11
AGTACAT81550.018.9743292
CAGAGTA83750.016.26023910-11
Produced by FastQC (version 0.11.5)