##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921709_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3496282
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.28199698994532	31.0	31.0	34.0	26.0	34.0
2	30.336616153960122	31.0	31.0	34.0	26.0	34.0
3	30.398599140458348	33.0	31.0	34.0	26.0	34.0
4	33.84556480283913	37.0	35.0	37.0	30.0	37.0
5	33.83530733504906	37.0	35.0	37.0	30.0	37.0
6	33.87450154192368	37.0	35.0	37.0	30.0	37.0
7	33.77943283751139	37.0	35.0	37.0	29.0	37.0
8	33.75489906134574	37.0	35.0	37.0	29.0	37.0
9	35.30434844786547	39.0	37.0	39.0	29.0	39.0
10-11	35.195331354850666	39.0	35.0	39.0	28.5	39.0
12-13	35.07748845773882	39.0	35.0	39.0	27.5	39.0
14-15	36.2076495831858	40.0	37.0	41.0	27.0	41.0
16-17	36.14315264043346	40.0	36.0	41.0	27.0	41.0
18-19	36.10005400022081	40.0	36.5	41.0	27.0	41.0
20-21	36.02653003962495	40.0	36.0	41.0	27.0	41.0
22-23	35.922352230169075	40.0	36.0	41.0	27.0	41.0
24-25	35.810155330719894	40.0	36.0	41.0	27.0	41.0
26-27	35.662995147416595	40.0	36.0	41.0	26.5	41.0
28-29	35.52001755007176	40.0	36.0	41.0	25.0	41.0
30-31	35.37124608369691	40.0	35.5	41.0	25.0	41.0
32-33	35.19670624394714	39.0	35.0	41.0	24.5	41.0
34-35	35.02275774665773	39.0	35.0	41.0	24.0	41.0
36-37	34.992763312570325	39.0	35.0	41.0	24.0	41.0
38-39	35.185416536766766	39.5	35.0	41.0	24.0	41.0
40-41	35.245181881781846	40.0	35.0	41.0	24.0	41.0
42-43	35.08615423469846	40.0	35.0	41.0	23.0	41.0
44-45	34.966888254437144	40.0	35.0	41.0	23.0	41.0
46-47	34.80595286650219	40.0	35.0	41.0	21.5	41.0
48-49	34.64194607299983	39.0	35.0	41.0	20.5	41.0
50-51	32.95381679738648	37.0	32.5	39.5	17.0	40.0
52-53	33.06578330924107	37.5	32.5	39.5	15.5	40.0
54-55	33.970666982812034	38.5	34.0	40.5	17.5	41.0
56-57	33.989278610821444	39.0	34.0	41.0	16.0	41.0
58-59	33.795915632663494	38.0	34.0	41.0	14.0	41.0
60-61	33.537793576147465	38.0	34.0	40.0	13.0	41.0
62-63	33.24199749905757	37.0	33.0	40.0	9.5	41.0
64-65	32.95260551065389	37.0	33.0	40.0	7.5	41.0
66-67	32.61415926976142	36.0	33.0	39.0	6.5	41.0
68-69	32.25756761039298	36.0	33.0	39.0	2.0	41.0
70-71	31.851155455995826	35.0	32.0	39.0	2.0	40.5
72-73	31.497940240518357	35.0	32.0	37.5	2.0	40.0
74-75	31.10975015745297	35.0	32.0	37.0	2.0	39.0
76-77	30.745791243383685	35.0	31.5	36.5	2.0	39.0
78-79	30.411864374784415	35.0	31.0	36.0	2.0	38.5
80-81	30.104801042936465	35.0	31.0	35.5	2.0	37.0
82-83	29.798192193879096	35.0	31.0	35.0	2.0	37.0
84-85	29.543876752504517	35.0	31.0	35.0	2.0	36.0
86-87	29.316305292307657	35.0	30.5	35.0	2.0	36.0
88-89	29.098530810729798	35.0	30.0	35.0	2.0	36.0
90-91	28.879868671920626	34.0	30.0	35.0	2.0	35.5
92-93	28.689113749977835	34.0	30.0	35.0	2.0	35.0
94-95	28.465355912366334	34.0	30.0	35.0	2.0	35.0
96-97	28.230052667376373	34.0	29.0	35.0	2.0	35.0
98-99	27.969172681151008	34.0	29.0	35.0	2.0	35.0
100-101	26.905736150573667	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	131520.0
3	22442.0
4	15108.0
5	11246.0
6	10506.0
7	10314.0
8	11049.0
9	11637.0
10	12677.0
11	13279.0
12	13239.0
13	13562.0
14	14214.0
15	14678.0
16	14819.0
17	15657.0
18	14923.0
19	14345.0
20	14398.0
21	15175.0
22	16739.0
23	18985.0
24	22168.0
25	26291.0
26	31574.0
27	36812.0
28	44048.0
29	51464.0
30	61667.0
31	76195.0
32	94744.0
33	123077.0
34	173912.0
35	250606.0
36	416405.0
37	746584.0
38	773942.0
39	136256.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.67373963557279	17.566393143560283	12.283414770247743	25.47645245061918
2	16.091816506661154	19.336099727577523	39.018494847213866	25.553588918547455
3	19.042092486181108	23.74714112884876	29.033137003430788	28.177629381539344
4	13.27698839853404	15.100051374445291	36.106904639584656	35.516055587436014
5	14.35368602893049	37.20621539343905	34.13294815058188	14.307150427048576
6	34.228357628321014	34.69527334721618	16.94097691022678	14.135392114236023
7	29.487776167925546	31.348247271395817	20.988662222330287	18.17531433834835
8	27.284666479772525	34.556746211102656	19.717770772425865	18.440816536698954
9	27.501774135464718	14.977622009176592	18.806722636195108	38.71388121916358
10-11	26.122743324999103	24.81594107940518	27.650952629616455	21.41036296597926
12-13	26.49247620146979	22.190438877638844	27.540625679883934	23.776459241007437
14-15	23.662642018528306	23.710668834573585	24.509999864088545	28.11668928280956
16-17	24.1196063663991	26.776955025666005	25.323995299513	23.779443308421897
18-19	24.0989404852486	26.342528761935274	26.41461237893099	23.143918373885136
20-21	24.565899648171133	25.565987902190518	26.287897198514187	23.580215251124166
22-23	24.454140350360174	25.294554986959454	26.057603294706915	24.193701367973457
24-25	24.261518266486302	25.48585981006791	26.008556442881897	24.244065480563894
26-27	24.034521083264554	25.692016552497538	26.083643609222595	24.189818755015313
28-29	24.13541080555207	25.97996882590248	25.840503499720096	24.04411686882536
30-31	24.471917305470996	25.687929186974056	25.772357770089943	24.06779573746501
32-33	24.252692780269616	25.702062246842328	25.69594091022273	24.349304062665325
34-35	24.482028642847112	25.77498697831378	25.841389712109898	23.901594666729213
36-37	24.522968965989666	25.621013419764715	25.85399087768333	24.002026736562286
38-39	24.391000485105877	25.74466326329808	25.797135358268996	24.067200893327048
40-41	24.443856829806954	25.57671524402827	25.86674072708588	24.1126871990789
42-43	24.2514710030108	25.54927573452695	25.74496041018397	24.45429285227828
44-45	24.17134498826784	25.616627931983416	25.92332466942989	24.28870241031886
46-47	24.358611740843237	25.573858623060257	25.747575853706135	24.319953782390368
48-49	24.14755251058193	25.721311642195094	25.77569185748343	24.355443989739552
50-51	24.20239932496687	25.719389850976032	25.88773261397811	24.190478210078986
52-53	24.379440360374456	25.586205305512845	25.868620142229094	24.165734191883608
54-55	23.887276985772857	25.418889829649967	26.204505728126865	24.489327456450315
56-57	23.993066416842925	25.531370996134427	25.990204314875925	24.48535827214672
58-59	24.16162501458112	25.524454580133966	25.98613511044822	24.327785294836694
60-61	24.158454435822787	25.533122047376683	26.12411604915802	24.18430746764251
62-63	24.184374429978792	25.490452874640685	26.110807895849593	24.21436479953093
64-65	23.967878027751944	25.352193805928415	26.09239652271798	24.58753164360166
66-67	24.019044324852235	25.487517206763577	25.947520069602227	24.54591839878196
68-69	24.052308726999662	25.402738254772	26.088111471902565	24.45684154632577
70-71	23.998999992120282	25.288193531610926	26.044087739946377	24.668718736322422
72-73	23.971441847682883	25.551230140347776	25.864204633134374	24.61312337883497
74-75	23.95106340451353	25.58482412470708	26.000843932035533	24.46326853874386
76-77	23.99709154470496	25.20700894744006	26.05526065060569	24.74063885724929
78-79	23.976225468560667	25.274031610720638	26.070117585827795	24.679625334890904
80-81	24.17084766011504	25.426621845193942	25.84890122804262	24.5536292666484
82-83	24.106580479879295	25.467069224543913	25.95366132141004	24.47268897416675
84-85	24.038782182047314	25.465864677945582	25.947052178420275	24.54830096158683
86-87	24.012187766596643	25.562920248043813	25.888454817315363	24.536437168044177
88-89	23.928338684718675	25.341101548860944	25.994575855116885	24.735983911303492
90-91	24.09703208111214	25.22989765238446	26.000310452666074	24.67275981383733
92-93	24.175131177827115	25.134932931260096	26.116821003358094	24.573114887554695
94-95	24.14070322660725	25.00234343892998	26.15226247203998	24.704690862422794
96-97	24.05537308473727	24.89731895469019	26.15575124224929	24.89155671832325
98-99	23.892416402700338	24.907638062749932	26.167911249982083	25.032034284567644
100-101	24.19187709499609	24.84701018928695	26.164070669230533	24.797042046486425
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	59.0
1	50.5
2	83.0
3	125.0
4	117.5
5	127.5
6	193.5
7	372.0
8	570.0
9	645.5
10	614.5
11	555.5
12	589.5
13	686.0
14	780.5
15	908.0
16	1035.5
17	1114.0
18	1208.5
19	1339.5
20	1474.0
21	1668.0
22	1840.0
23	2008.5
24	2318.0
25	2867.0
26	3678.0
27	4842.0
28	6580.0
29	9355.5
30	13240.5
31	18648.5
32	26414.5
33	32865.5
34	38756.0
35	46037.0
36	55244.5
37	65156.0
38	74825.5
39	83745.0
40	91362.0
41	99493.0
42	106830.0
43	114452.5
44	122332.5
45	130748.5
46	136837.5
47	142225.5
48	148850.5
49	152453.0
50	152424.0
51	151658.5
52	151117.0
53	150369.5
54	149117.5
55	143056.5
56	133228.0
57	122017.0
58	110214.0
59	96559.5
60	80641.0
61	67507.5
62	56295.0
63	43605.5
64	32306.0
65	24387.5
66	18748.0
67	14991.0
68	11932.5
69	9147.5
70	7013.0
71	5300.5
72	3821.0
73	2910.5
74	2391.0
75	1867.0
76	1443.0
77	1020.5
78	665.5
79	465.5
80	349.5
81	264.0
82	195.5
83	156.5
84	127.0
85	103.5
86	80.5
87	69.5
88	59.5
89	55.5
90	54.5
91	53.0
92	47.0
93	37.0
94	30.0
95	21.5
96	18.0
97	16.5
98	13.0
99	10.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05025338345133487
2	0.06990282820436108
3	0.06672802708705991
4	0.06655641621585444
5	0.062437755306923186
6	0.010353855896063304
7	0.01278500990480745
8	0.015502182032227377
9	0.006092185927794154
10-11	0.013113930741284599
12-13	0.021179641687941647
14-15	0.03888416323397255
16-17	0.02572732977488658
18-19	0.00978181965871174
20-21	0.01974955109456274
22-23	0.034937113196246754
24-25	0.032548861905303976
26-27	0.021079535346405123
28-29	0.0123273809149262
30-31	0.012241575479323466
32-33	0.008966668020485761
34-35	0.005462946066707434
36-37	6.006380492191419E-4
38-39	0.00394705003772579
40-41	0.001072567945034182
42-43	0.21468519987804185
44-45	0.18635510522320567
46-47	0.1917179449483766
48-49	0.19911151331614557
50-51	0.1909313951220182
52-53	0.19768142272276662
54-55	0.19422060348678968
56-57	0.19702358104981232
58-59	0.20538961102107897
60-61	0.20963698008341433
62-63	0.19530747233775766
64-65	0.19542187958522797
66-67	0.1927333092696756
68-69	0.18812841755899554
70-71	0.18046313197848457
72-73	0.19164644041870765
74-75	0.19061677519147482
76-77	0.18468189922895237
78-79	0.20267243889365905
80-81	0.21388434914574966
82-83	0.21238275402270185
84-85	0.20609035541183465
86-87	0.22498185215037003
88-89	0.21798870914874713
90-91	0.22388068239346826
92-93	0.2201481459447493
94-95	0.22363756699259385
96-97	0.23033039096960717
98-99	0.22400939054687238
100-101	0.2305163027467464
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3496282.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.84136819664403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.62909758341482	44.90173216095506
2	11.983698120887045	13.863069907353173
3	4.139633197748595	7.183261439700823
4	1.9633685011795594	4.542556815296801
5	1.0954627181475871	3.168153121303553
6	0.6910512946120703	2.398281142465457
7	0.4525818331176289	1.8324566713928212
8	0.3444076639615467	1.59368084007567
9	0.25002873949052207	1.301580394255267
>10	1.3608947252200665	13.863198263383067
>50	0.06860459759078634	2.6839200537081482
>100	0.019634290622611215	1.8375337512978078
>500	8.922971675724367E-4	0.36945367332834694
>1k	6.444368395250031E-4	0.4611217654841088
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.720362373515637E-5
2	0.0	0.0	2.8601811867578187E-5	0.0	5.720362373515637E-5
3	0.0	2.8601811867578187E-5	2.8601811867578187E-5	0.0	5.720362373515637E-5
4	0.0	2.8601811867578187E-5	2.8601811867578187E-5	0.0	5.720362373515637E-5
5	0.0	2.8601811867578187E-5	2.8601811867578187E-5	0.0	5.720362373515637E-5
6	0.0	2.8601811867578187E-5	2.8601811867578187E-5	0.0	8.580543560273456E-5
7	0.0	2.8601811867578187E-5	2.8601811867578187E-5	0.0	8.580543560273456E-5
8	0.0	2.8601811867578187E-5	2.8601811867578187E-5	0.0	8.580543560273456E-5
9	0.0	2.8601811867578187E-5	2.8601811867578187E-5	2.8601811867578187E-5	8.580543560273456E-5
10-11	0.0	2.8601811867578187E-5	2.8601811867578187E-5	4.290271780136728E-5	1.0010634153652365E-4
12-13	0.0	2.8601811867578187E-5	2.8601811867578187E-5	5.720362373515637E-5	1.859117771392582E-4
14-15	0.0	2.8601811867578187E-5	2.8601811867578187E-5	5.720362373515637E-5	2.8601811867578185E-4
16-17	0.0	2.8601811867578187E-5	2.8601811867578187E-5	5.720362373515637E-5	3.1461993054336006E-4
18-19	0.0	2.8601811867578187E-5	2.8601811867578187E-5	5.720362373515637E-5	3.432217424109382E-4
20-21	0.0	2.8601811867578187E-5	2.8601811867578187E-5	8.580543560273456E-5	4.147262720798837E-4
22-23	0.0	2.8601811867578187E-5	2.8601811867578187E-5	1.1440724747031275E-4	4.719298958150401E-4
24-25	0.0	2.8601811867578187E-5	2.8601811867578187E-5	2.145135890068364E-4	5.291335195501965E-4
26-27	0.0	2.8601811867578187E-5	2.8601811867578187E-5	7.007443907556656E-4	5.720362373515638E-4
28-29	0.0	2.8601811867578187E-5	2.8601811867578187E-5	0.003046092963897077	5.720362373515638E-4
30-31	0.0	2.8601811867578187E-5	2.8601811867578187E-5	0.013256939800622489	5.720362373515638E-4
32-33	0.0	2.8601811867578187E-5	2.8601811867578187E-5	0.0360668847650161	5.720362373515638E-4
34-35	0.0	2.8601811867578187E-5	2.8601811867578187E-5	0.06585567182509877	6.006380492191419E-4
36-37	0.0	2.8601811867578187E-5	2.8601811867578187E-5	0.11569432900435378	6.006380492191419E-4
38-39	0.0	2.8601811867578187E-5	2.8601811867578187E-5	0.19818195443044925	6.14938955152931E-4
40-41	0.0	2.8601811867578187E-5	2.8601811867578187E-5	0.2913952593068866	6.292398610867201E-4
42-43	0.0	2.8601811867578187E-5	2.8601811867578187E-5	0.3877118607709561	6.292398610867201E-4
44-45	0.0	2.8601811867578187E-5	2.8601811867578187E-5	0.5003743977173466	6.721425788880873E-4
46-47	0.0	2.8601811867578187E-5	2.8601811867578187E-5	0.630469738997026	7.72248920424611E-4
48-49	0.0	2.8601811867578187E-5	2.8601811867578187E-5	0.7738649227951293	8.866561678949238E-4
50-51	0.0	2.8601811867578187E-5	2.8601811867578187E-5	0.9263697836730562	8.866561678949238E-4
52-53	0.0	2.8601811867578187E-5	2.8601811867578187E-5	1.0814059049012636	9.867625094314475E-4
54-55	0.0	2.8601811867578187E-5	2.8601811867578187E-5	1.255590939174815	0.001072567945034182
56-57	0.0	2.8601811867578187E-5	2.8601811867578187E-5	1.4544450361841523	0.0011154706628355494
58-59	0.0	4.290271780136728E-5	2.8601811867578187E-5	1.6642822289506396	0.0011583733806369166
60-61	0.0	5.720362373515637E-5	2.8601811867578187E-5	1.8936687601286166	0.0011726742865707056
62-63	0.0	5.720362373515637E-5	2.8601811867578187E-5	2.1301342397438194	0.0012012760984382838
64-65	0.0	5.720362373515637E-5	2.8601811867578187E-5	2.3767533625720123	0.0012012760984382838
66-67	0.0	8.580543560273456E-5	2.8601811867578187E-5	2.66045473448652	0.0012012760984382838
68-69	0.0	8.580543560273456E-5	2.8601811867578187E-5	2.976647764682597	0.0012012760984382838
70-71	2.8601811867578187E-5	1.0010634153652365E-4	2.8601811867578187E-5	3.306912886317522	0.001229877910305862
72-73	2.8601811867578187E-5	1.1440724747031275E-4	2.8601811867578187E-5	3.647703474719717	0.0014014887815113311
74-75	2.8601811867578187E-5	1.4300905933789095E-4	2.8601811867578187E-5	3.9983616882162254	0.0014157896874451201
76-77	2.8601811867578187E-5	1.4300905933789095E-4	2.8601811867578187E-5	4.3858876372100415	0.0014300905933789093
78-79	2.8601811867578187E-5	1.4300905933789095E-4	2.8601811867578187E-5	4.8259122118867985	0.0014300905933789093
80-81	2.8601811867578187E-5	1.4300905933789095E-4	2.8601811867578187E-5	5.2830120682485	0.0015015951230478548
82-83	2.8601811867578187E-5	1.4300905933789095E-4	2.8601811867578187E-5	5.755900124761103	0.0016446041823857459
84-85	2.8601811867578187E-5	1.4300905933789095E-4	2.8601811867578187E-5	6.249710406654841	0.0016589050883195349
86-87	2.8601811867578187E-5	1.4300905933789095E-4	2.8601811867578187E-5	6.766616651631647	0.001687506900187113
88-89	2.8601811867578187E-5	1.4300905933789095E-4	2.8601811867578187E-5	7.3321459767833375	0.0017161087120546913
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2435	0.0	45.221313	1
GTATCAA	4440	0.0	44.79043	1
TCAACGC	5540	0.0	34.95762	4
CAACGCA	5685	0.0	34.062584	5
GTACATG	10810	0.0	32.446877	1
ATCAACG	5995	0.0	32.14426	3
TATCAAC	6180	0.0	31.566479	2
AACGCAG	6195	0.0	31.317535	6
TACATGG	11510	0.0	29.856304	2
ACATGGG	11865	0.0	28.402542	3
CATGGGG	10065	0.0	25.843908	4
ACGCAGA	7435	0.0	25.269365	7
CGCAGAG	7905	0.0	23.708286	8
ATGGGGG	6145	0.0	22.244291	5
GAGTACA	7945	0.0	20.84902	1
GTATAGG	940	0.0	19.692049	1
GCAGAGT	9110	0.0	19.688074	9
AGAGTAC	7645	0.0	19.116266	10-11
AGTACAT	8155	0.0	18.974329	2
CAGAGTA	8375	0.0	16.260239	10-11
>>END_MODULE