FastQCFastQC Report
Mon 15 Aug 2016
SRR2921709_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921709_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3496282
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25000.048.1083031
GTATCAA46800.041.443251
TCAACGC56100.033.2485584
CAACGCA56900.032.639015
ATCAACG58200.032.1304173
TATCAAC61050.030.9414422
AACGCAG61100.030.545646
GTACATG101750.029.1534331
TACATGG106100.027.4213092
ACATGGG109400.025.9434073
ACGCAGA74600.024.5723347
CATGGGG94450.023.115274
CGCAGAG79650.022.597038
ATGGGGG57550.020.7157635
GAGTACA74400.018.7212221
GCAGAGT90950.018.4321569
AGAGTAC75550.017.78918610-11
AGTACAT77600.017.0030252
CAGAGTA82800.016.2315610-11
TAAGGCG10150.015.9105575
Produced by FastQC (version 0.11.5)