##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921709_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3496282
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21723219122485	33.0	31.0	34.0	28.0	34.0
2	31.35078749368615	34.0	31.0	34.0	28.0	34.0
3	31.42064084075598	34.0	31.0	34.0	28.0	34.0
4	34.900179390564034	37.0	35.0	37.0	32.0	37.0
5	34.619990321146865	37.0	35.0	37.0	32.0	37.0
6	34.58812161032777	37.0	35.0	37.0	32.0	37.0
7	34.46503542906436	37.0	35.0	37.0	31.0	37.0
8	34.40886404471951	37.0	35.0	37.0	31.0	37.0
9	35.98489252297154	39.0	37.0	39.0	31.0	39.0
10-11	35.86708609316983	39.0	37.0	39.0	30.0	39.0
12-13	35.774786044146325	39.0	37.0	39.0	30.0	39.0
14-15	36.99686967470015	40.0	38.0	41.0	31.0	41.0
16-17	36.95670729649382	40.0	38.0	41.0	31.0	41.0
18-19	36.91035763133523	40.0	37.5	41.0	30.5	41.0
20-21	36.849631980486706	40.0	37.0	41.0	30.0	41.0
22-23	36.755915569739514	40.0	37.0	41.0	30.0	41.0
24-25	36.66831050813407	40.0	37.0	41.0	30.0	41.0
26-27	36.53382421669648	40.0	37.0	41.0	30.0	41.0
28-29	36.41088047245617	40.0	37.0	41.0	29.5	41.0
30-31	36.24764249565682	40.0	36.5	41.0	29.0	41.0
32-33	36.2001414645615	40.0	36.5	41.0	28.5	41.0
34-35	36.33382490313997	40.0	37.0	41.0	30.0	41.0
36-37	36.329981963697435	40.0	37.0	41.0	29.0	41.0
38-39	36.2237824351697	40.0	36.5	41.0	29.0	41.0
40-41	36.1367874502114	40.0	36.0	41.0	28.5	41.0
42-43	36.01495073909942	40.0	36.0	41.0	28.0	41.0
44-45	35.884274781038826	40.0	36.0	41.0	27.5	41.0
46-47	35.72850001801914	40.0	35.5	41.0	27.0	41.0
48-49	35.580142419862014	40.0	35.0	41.0	26.5	41.0
50-51	35.42065385458038	39.5	35.0	41.0	26.0	41.0
52-53	35.24204669417398	39.0	35.0	41.0	26.0	41.0
54-55	35.01925259461336	39.0	35.0	41.0	25.5	41.0
56-57	34.79412916349425	39.0	35.0	41.0	25.0	41.0
58-59	34.54918682188679	38.0	34.5	40.0	24.0	41.0
60-61	34.26617918691913	38.0	34.0	40.0	23.5	41.0
62-63	33.95721912591719	37.0	34.0	40.0	23.0	41.0
64-65	33.621629920012175	37.0	34.0	40.0	22.0	41.0
66-67	33.27299299656035	36.0	34.0	39.0	22.0	41.0
68-69	32.91606383581187	36.0	33.0	39.0	21.0	40.5
70-71	32.52277733889886	35.0	33.0	38.5	20.0	40.0
72-73	32.16931743492087	35.0	33.0	37.0	19.0	39.5
74-75	31.777569429468215	35.0	32.5	37.0	17.5	39.0
76-77	29.10770183869608	33.0	28.5	34.5	9.5	36.5
78-79	30.674777520806387	34.5	31.0	35.5	11.0	37.0
80-81	30.864569419743603	35.0	32.0	35.5	11.0	37.0
82-83	30.698649451045426	35.0	32.0	35.0	8.0	37.0
84-85	30.505965193883103	35.0	32.0	35.0	7.0	36.0
86-87	30.30651188891514	35.0	32.0	35.0	2.0	36.0
88-89	30.047425808330104	35.0	31.0	35.0	2.0	36.0
90-91	29.853320613154203	34.0	31.0	35.0	2.0	35.0
92-93	29.69746905426965	34.0	31.0	35.0	2.0	35.0
94-95	29.593469720119828	34.0	31.0	35.0	2.0	35.0
96-97	29.392742204433166	34.0	31.0	35.0	2.0	35.0
98-99	29.197822715673393	34.0	31.0	35.0	2.0	35.0
100-101	28.093569397434187	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79216.0
3	26406.0
4	14106.0
5	9611.0
6	8381.0
7	8352.0
8	8358.0
9	9085.0
10	10001.0
11	10600.0
12	11213.0
13	12107.0
14	12691.0
15	13275.0
16	14398.0
17	15413.0
18	14197.0
19	12770.0
20	12636.0
21	12481.0
22	13103.0
23	15315.0
24	17970.0
25	21468.0
26	25911.0
27	31199.0
28	38050.0
29	45405.0
30	55258.0
31	69086.0
32	87735.0
33	116014.0
34	167937.0
35	251408.0
36	439909.0
37	841171.0
38	844211.0
39	99832.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.200165802467616	19.3042999500702	12.958746180427344	26.536788067034845
2	15.169126948964884	20.77986339190894	37.30271525813586	26.748294400990307
3	17.569610867939726	24.9866559265707	28.904922118094905	28.538811087394674
4	12.942157067176268	16.46044945883536	35.019197264586445	35.57819620940192
5	14.08292511415499	37.53878952368883	34.232793500839804	14.145491861316383
6	34.28882377084933	35.33933124822129	16.59551829621462	13.776326684714757
7	29.290829515468147	31.528206248809447	21.026478985390767	18.154485250331636
8	27.212278643427506	35.04245366935505	19.438763806809632	18.306503880407814
9	27.193674419668906	14.897662658597138	18.783364032628967	39.12529888910499
10-11	26.002208482714	25.111790487729014	27.620470042266774	21.26553098729021
12-13	26.306714386107018	22.371905594220582	27.66278853257573	23.658591487096672
14-15	23.492652579641746	23.886946261932852	24.538910937108945	28.081490221316457
16-17	23.99374878710424	27.034608054299973	25.295174315912494	23.676468842683292
18-19	24.0291336418368	26.50855935516884	26.407479135400873	23.05482786759349
20-21	24.4758880996513	25.700750070467926	26.2885047685799	23.534857061300873
22-23	24.35556175897164	25.4147299066165	26.073501232666764	24.156207101745096
24-25	24.11344038843704	25.651480378506452	26.080008740748706	24.155070492307797
26-27	23.94029049875379	25.86705714843055	26.109477906546374	24.083174446269286
28-29	24.0460176348208	26.109920655748866	25.911012988922355	23.933048720507983
30-31	24.393396890165437	25.807055577876525	25.800862283883717	23.998685248074324
32-33	24.139252784772506	25.819884910467643	25.75666776414684	24.28419454061301
34-35	24.31588945419471	25.931432835961783	25.881088787307693	23.871588922535814
36-37	24.397717609525195	25.75400603919902	25.927138897286994	23.92113745398879
38-39	24.321279004382372	25.876502524681932	25.84740018110667	23.954818289829024
40-41	24.383913614974595	25.66792180509968	25.87983993302369	24.068324646902038
42-43	24.167623840075308	25.716235305761302	25.745325940250353	24.370814913913033
44-45	24.09114968701119	25.694129505815344	25.929981294611938	24.28473951256153
46-47	24.293177379216292	25.75765701563989	25.675552567512355	24.273613037631467
48-49	24.047021146415045	25.86683653898687	25.78917544513537	24.296966869462715
50-51	24.08344909024577	25.857222065782153	25.866060254422564	24.193268589549515
52-53	24.370287857988362	25.68798220821599	25.71435354263268	24.227376391162974
54-55	23.797357997514972	25.606026195777282	26.10111563165735	24.4955001750504
56-57	23.92993006478936	25.701372259867394	25.849712308691466	24.518985366651776
58-59	24.02119085644781	25.646420098902865	25.904032693385375	24.428356351263947
60-61	24.079256139531903	25.7055856468133	25.997745443977628	24.217412769677164
62-63	24.062482567046246	25.60343409521003	25.997322298303406	24.33676103944031
64-65	23.793962979913655	25.51759549272916	25.968582956842805	24.71985857051438
66-67	23.84823247160344	25.679109191216348	25.798493663593646	24.674164673586567
68-69	23.89148221834475	25.611578625814396	25.888524749663226	24.608414406177634
70-71	23.777627420574476	25.440023790020444	25.895345662242384	24.887003127162696
72-73	23.709556421017215	25.725326426934043	25.692795317809193	24.87232183423955
74-75	23.620616997043786	25.726071988805693	25.792903655686455	24.860407358464062
76-77	23.611354563600724	25.35088331570204	25.936478320673245	25.101283800023992
78-79	23.512291788976764	25.43293838204603	25.987480789383515	25.067289039593692
80-81	23.676691557168496	25.563785607775678	25.718308972315164	25.041213862740662
82-83	23.553585064037954	25.56605935487415	25.826634193037663	25.05372138805024
84-85	23.451639174909616	25.552525080826964	25.804993100590583	25.190842643672834
86-87	23.358847098010806	25.673174427314105	25.78991972079586	25.178058753879228
88-89	23.21971877165296	25.45046405015118	25.88186309656394	25.447954081631924
90-91	23.221332903415696	25.408548457101514	25.915743218685122	25.454375420797664
92-93	23.29343006038931	25.201730510615665	26.030658087083825	25.4741813419112
94-95	23.13614667058359	25.129686038931414	26.11116657222386	25.623000718261142
96-97	22.94366257416611	25.04653819478861	26.16638697265235	25.843412258392927
98-99	22.746696107692372	25.024347480375432	26.135865375952267	26.093091035979928
100-101	22.762368520782832	25.04532681210717	26.12486145969639	26.067443207413604
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	60.0
1	52.0
2	69.5
3	99.0
4	89.0
5	101.5
6	173.0
7	359.0
8	525.5
9	539.0
10	464.0
11	401.0
12	405.0
13	440.5
14	461.0
15	516.0
16	594.0
17	636.5
18	749.0
19	874.0
20	970.5
21	1140.0
22	1369.5
23	1680.5
24	2084.5
25	2688.0
26	3681.5
27	5145.0
28	7230.0
29	10200.5
30	14312.5
31	19993.5
32	27848.0
33	34203.0
34	39402.0
35	46532.0
36	55905.0
37	65849.5
38	74917.5
39	83547.0
40	91700.5
41	99802.0
42	107030.0
43	114908.0
44	123483.5
45	131599.5
46	137988.0
47	143960.0
48	150091.0
49	153725.0
50	154109.5
51	153102.0
52	152334.0
53	151601.0
54	149213.5
55	142562.0
56	132978.0
57	121953.0
58	109996.0
59	95661.0
60	79628.5
61	66372.5
62	54800.5
63	42166.5
64	30815.5
65	22938.5
66	17604.0
67	14103.5
68	11257.5
69	8465.0
70	6410.5
71	4851.0
72	3483.5
73	2649.0
74	2091.5
75	1554.5
76	1136.0
77	717.5
78	424.5
79	275.0
80	170.0
81	102.0
82	56.5
83	30.5
84	26.0
85	18.0
86	10.0
87	9.5
88	7.0
89	4.5
90	5.5
91	5.5
92	3.0
93	3.0
94	2.0
95	1.0
96	1.5
97	1.5
98	1.5
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15390634965943822
2	8.580543560273456E-5
3	0.00995343052991721
4	0.006034982304058997
5	0.02331047667207622
6	0.0019163213951277386
7	0.0
8	0.0
9	5.720362373515637E-5
10-11	0.045047853691435644
12-13	3.7182355427851644E-4
14-15	0.0044904844632097755
16-17	1.4300905933789093E-5
18-19	0.0014014887815113311
20-21	3.0031902460957095E-4
22-23	1.4300905933789093E-5
24-25	4.004253661460946E-4
26-27	0.0025169594443468805
28-29	0.005362839725170911
30-31	0.016174324611115464
32-33	0.011297715687693385
34-35	0.009653111505307638
36-37	0.003518022859712117
38-39	0.0
40-41	0.0034465183300431716
42-43	0.008909464396750605
44-45	0.0065927176354767724
46-47	0.003589527389381063
48-49	0.009238385233227755
50-51	0.002588463974015826
52-53	0.0017590114298560585
54-55	0.004175864532666415
56-57	0.007450771991504118
58-59	0.015130358477948863
60-61	0.007150452966894548
62-63	0.021365553465080907
64-65	0.03991382846120536
66-67	0.060321221228722396
68-69	0.04975285174365226
70-71	0.06807231224483609
72-73	0.03334971263759617
74-75	0.026685490472450446
76-77	0.22004803960321279
78-79	0.07925562068505916
80-81	0.06612738903784077
82-83	0.026685490472450446
84-85	0.050911225124289174
86-87	0.06724285970067632
88-89	0.2912379493416149
90-91	0.29582854014636123
92-93	0.04880899195202218
94-95	0.009696014223109006
96-97	0.014229401404120148
98-99	7.722489204246111E-4
100-101	2.002126830730473E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3496282.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.12547882530259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.1332330333906	44.062728703760854
2	12.236325612618296	13.980119193662684
3	4.197837239125234	7.194103869471458
4	2.005712711371722	4.583091960924222
5	1.1298873550941269	3.227267808920335
6	0.6889362768040866	2.3613488815532797
7	0.4790929256492714	1.9157888945670807
8	0.34758801099868986	1.5884905249787762
9	0.26305215046585806	1.3524282046248919
>10	1.427205897145331	14.409341733881684
>50	0.07042188719314985	2.7321669913134046
>100	0.019147959980152483	1.7820601517573014
>500	0.0010057678500665298	0.4208576545683797
>1k	5.53172313439992E-4	0.39020542601567815
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.8601811867578187E-5
2	0.0	0.0	0.0	0.0	2.8601811867578187E-5
3	0.0	0.0	0.0	0.0	2.8601811867578187E-5
4	0.0	0.0	0.0	0.0	2.8601811867578187E-5
5	0.0	0.0	0.0	0.0	2.8601811867578187E-5
6	0.0	0.0	0.0	0.0	2.8601811867578187E-5
7	0.0	0.0	0.0	0.0	2.8601811867578187E-5
8	0.0	0.0	0.0	0.0	2.8601811867578187E-5
9	0.0	0.0	0.0	0.0	5.720362373515637E-5
10-11	0.0	0.0	0.0	1.4300905933789093E-5	5.720362373515637E-5
12-13	0.0	0.0	0.0	2.8601811867578187E-5	1.7161087120546914E-4
14-15	0.0	1.4300905933789093E-5	0.0	2.8601811867578187E-5	2.717172127419928E-4
16-17	0.0	2.8601811867578187E-5	0.0	2.8601811867578187E-5	3.2892083647714917E-4
18-19	0.0	2.8601811867578187E-5	0.0	2.8601811867578187E-5	4.004253661460946E-4
20-21	0.0	5.720362373515637E-5	0.0	5.720362373515637E-5	4.004253661460946E-4
22-23	0.0	5.720362373515637E-5	0.0	1.1440724747031275E-4	4.290271780136728E-4
24-25	0.0	5.720362373515637E-5	0.0	2.4311540087441457E-4	4.862308017488292E-4
26-27	0.0	5.720362373515637E-5	0.0	7.57948014490822E-4	4.862308017488292E-4
28-29	0.0	5.720362373515637E-5	0.0	0.003131898399499812	5.148326136164074E-4
30-31	0.0	5.720362373515637E-5	0.0	0.013428550671827959	5.434344254839856E-4
32-33	0.0	5.720362373515637E-5	0.0	0.036796230967639336	5.434344254839856E-4
34-35	2.8601811867578187E-5	5.720362373515637E-5	0.0	0.06705694792353706	5.720362373515638E-4
36-37	2.8601811867578187E-5	5.720362373515637E-5	0.0	0.11715302140960025	5.720362373515638E-4
38-39	2.8601811867578187E-5	5.720362373515637E-5	0.0	0.19999816948404048	6.006380492191419E-4
40-41	2.8601811867578187E-5	5.720362373515637E-5	0.0	0.2936977051622266	6.006380492191419E-4
42-43	2.8601811867578187E-5	5.720362373515637E-5	0.0	0.39037182927464087	6.14938955152931E-4
44-45	2.8601811867578187E-5	5.720362373515637E-5	0.0	0.502519533607415	6.292398610867201E-4
46-47	2.8601811867578187E-5	5.720362373515637E-5	0.0	0.6324146622040213	6.435407670205092E-4
48-49	2.8601811867578187E-5	5.720362373515637E-5	0.0	0.7753379161063095	6.864434848218765E-4
50-51	2.8601811867578187E-5	5.720362373515637E-5	0.0	0.9275567588655607	6.864434848218765E-4
52-53	5.720362373515637E-5	5.720362373515637E-5	0.0	1.0814774094309327	7.007443907556656E-4
54-55	5.720362373515637E-5	5.720362373515637E-5	0.0	1.2556767446104176	7.436471085570329E-4
56-57	5.720362373515637E-5	5.720362373515637E-5	0.0	1.4545451425256888	7.436471085570329E-4
58-59	5.720362373515637E-5	7.150452966894546E-5	0.0	1.6642107244209705	7.436471085570329E-4
60-61	5.720362373515637E-5	8.580543560273456E-5	0.0	1.8933541401980734	7.436471085570329E-4
62-63	5.720362373515637E-5	8.580543560273456E-5	0.0	2.1285611400911026	8.294525441597674E-4
64-65	5.720362373515637E-5	8.580543560273456E-5	0.0	2.3743651112810698	8.294525441597674E-4
66-67	5.720362373515637E-5	8.580543560273456E-5	0.0	2.6569796143446096	8.294525441597674E-4
68-69	5.720362373515637E-5	8.580543560273456E-5	0.0	2.971771155759175	8.580543560273456E-4
70-71	5.720362373515637E-5	8.580543560273456E-5	0.0	3.301449940250815	9.438597916300802E-4
72-73	5.720362373515637E-5	8.580543560273456E-5	0.0	3.641611288791922	0.0010296652272328147
74-75	7.150452966894546E-5	8.580543560273456E-5	0.0	3.9924268122537026	0.001043966133166604
76-77	8.580543560273456E-5	8.580543560273456E-5	0.0	4.381025329192553	0.001058267039100393
78-79	8.580543560273456E-5	8.580543560273456E-5	0.0	4.821893657319404	0.001058267039100393
80-81	8.580543560273456E-5	8.580543560273456E-5	0.0	5.282726050129824	0.0011011697569017604
82-83	8.580543560273456E-5	8.580543560273456E-5	0.0	5.756886887270534	0.0012012760984382838
84-85	8.580543560273456E-5	8.580543560273456E-5	0.0	6.253185526796751	0.0012012760984382838
86-87	8.580543560273456E-5	8.580543560273456E-5	0.0	6.772694536653508	0.0012155770043720728
88-89	8.580543560273456E-5	8.580543560273456E-5	0.0	7.341298556580963	0.001229877910305862
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2500	0.0	48.108303	1
GTATCAA	4680	0.0	41.44325	1
TCAACGC	5610	0.0	33.248558	4
CAACGCA	5690	0.0	32.63901	5
ATCAACG	5820	0.0	32.130417	3
TATCAAC	6105	0.0	30.941442	2
AACGCAG	6110	0.0	30.54564	6
GTACATG	10175	0.0	29.153433	1
TACATGG	10610	0.0	27.421309	2
ACATGGG	10940	0.0	25.943407	3
ACGCAGA	7460	0.0	24.572334	7
CATGGGG	9445	0.0	23.11527	4
CGCAGAG	7965	0.0	22.59703	8
ATGGGGG	5755	0.0	20.715763	5
GAGTACA	7440	0.0	18.721222	1
GCAGAGT	9095	0.0	18.432156	9
AGAGTAC	7555	0.0	17.789186	10-11
AGTACAT	7760	0.0	17.003025	2
CAGAGTA	8280	0.0	16.23156	10-11
TAAGGCG	1015	0.0	15.910557	5
>>END_MODULE