FastQCFastQC Report
Mon 15 Aug 2016
SRR2921708_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921708_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2889908
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41000.045.2644921
GGTATCA21600.045.046891
TCAACGC49950.035.8249554
CAACGCA51800.034.449685
ATCAACG52350.034.3632933
TATCAAC53250.034.0516782
AACGCAG55400.032.2810946
GTACATG74900.028.389711
ACGCAGA63700.027.9298487
TACATGG76750.027.0272
CGCAGAG64750.026.8921538
ACATGGG80150.025.4646193
CATGGGG68650.023.1624264
GCAGAGT75600.021.6534299
AGAGTAC63550.019.67442110-11
TAAGGCG12550.019.2865075
ATGGGGG42750.018.5399045
GAGTACA57650.018.35991
GTATTAA21800.018.2888981
GTATAGG8650.017.5589521
Produced by FastQC (version 0.11.5)