##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921708_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2889908
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.49218660248008	31.0	31.0	34.0	27.0	34.0
2	30.5534809412618	33.0	31.0	34.0	26.0	34.0
3	30.610557844747998	33.0	31.0	34.0	26.0	34.0
4	34.0713863555518	37.0	35.0	37.0	30.0	37.0
5	34.06884406008773	37.0	35.0	37.0	30.0	37.0
6	34.111740927392844	37.0	35.0	37.0	30.0	37.0
7	34.02447655773125	37.0	35.0	37.0	30.0	37.0
8	33.99519431068394	37.0	35.0	37.0	30.0	37.0
9	35.566387926536066	39.0	37.0	39.0	30.0	39.0
10-11	35.46479939845836	39.0	37.0	39.0	30.0	39.0
12-13	35.363859160914465	39.0	37.0	39.0	30.0	39.0
14-15	36.516176639533164	40.0	37.0	41.0	29.0	41.0
16-17	36.45785540577762	40.0	37.0	41.0	29.0	41.0
18-19	36.428501530152516	40.0	37.0	41.0	29.0	41.0
20-21	36.35066081688413	40.0	37.0	41.0	28.0	41.0
22-23	36.25738708637091	40.0	37.0	41.0	28.0	41.0
24-25	36.145974716150135	40.0	36.5	41.0	27.0	41.0
26-27	35.99962836187173	40.0	36.0	41.0	27.0	41.0
28-29	35.85964086053951	40.0	36.0	41.0	27.0	41.0
30-31	35.71023991075148	40.0	36.0	41.0	26.5	41.0
32-33	35.53071862495277	40.0	35.5	41.0	25.5	41.0
34-35	35.35198058208081	39.0	35.0	41.0	25.0	41.0
36-37	35.31662495830317	39.0	35.0	41.0	25.0	41.0
38-39	35.485920313034185	40.0	35.5	41.0	25.5	41.0
40-41	35.54167883545082	40.0	35.0	41.0	25.0	41.0
42-43	35.37390688561712	40.0	35.0	41.0	24.5	41.0
44-45	35.245189639254946	40.0	35.0	41.0	24.0	41.0
46-47	35.08118182308918	40.0	35.0	41.0	23.5	41.0
48-49	34.910699060316105	39.5	35.0	41.0	23.0	41.0
50-51	33.21881457818034	37.0	32.5	39.5	18.5	40.0
52-53	33.33546881077183	37.5	33.5	39.5	19.0	40.0
54-55	34.240304362630226	39.0	34.0	40.5	20.5	41.0
56-57	34.25351862412229	39.0	34.0	41.0	20.0	41.0
58-59	34.060303476788874	38.0	34.0	41.0	19.5	41.0
60-61	33.80904963064568	38.0	34.0	40.0	18.0	41.0
62-63	33.511049486696464	37.0	34.0	40.0	16.5	41.0
64-65	33.2177597003088	37.0	33.5	40.0	13.5	41.0
66-67	32.87941761467839	36.0	33.0	39.5	12.0	41.0
68-69	32.520816579628146	36.0	33.0	39.0	8.0	41.0
70-71	32.10930417162069	35.0	33.0	39.0	7.0	40.5
72-73	31.754045457502453	35.0	32.0	37.5	2.0	40.0
74-75	31.362176408383935	35.0	32.0	37.0	2.0	39.0
76-77	30.991223423029382	35.0	32.0	36.5	2.0	39.0
78-79	30.650802378484023	35.0	32.0	36.0	2.0	38.5
80-81	30.338757496778445	35.0	31.0	36.0	2.0	37.0
82-83	30.02826664378243	35.0	31.0	35.0	2.0	37.0
84-85	29.7719353003625	35.0	31.0	35.0	2.0	36.5
86-87	29.537843419236875	35.0	31.0	35.0	2.0	36.0
88-89	29.31551246614079	35.0	31.0	35.0	2.0	36.0
90-91	29.092204492322942	35.0	30.5	35.0	2.0	35.5
92-93	28.901195297566566	34.0	30.0	35.0	2.0	35.0
94-95	28.674845012367175	34.0	30.0	35.0	2.0	35.0
96-97	28.44329923305517	34.0	30.0	35.0	2.0	35.0
98-99	28.1755557616367	34.0	30.0	35.0	2.0	35.0
100-101	27.109486876398833	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	93867.0
3	16632.0
4	11211.0
5	8678.0
6	7949.0
7	8343.0
8	8591.0
9	9260.0
10	10048.0
11	10906.0
12	10895.0
13	11003.0
14	12049.0
15	12835.0
16	12964.0
17	13323.0
18	12631.0
19	11977.0
20	12154.0
21	12577.0
22	13672.0
23	16028.0
24	18304.0
25	21510.0
26	25790.0
27	30222.0
28	35352.0
29	42369.0
30	50413.0
31	61985.0
32	76253.0
33	99595.0
34	138620.0
35	203169.0
36	343261.0
37	621677.0
38	664530.0
39	119256.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.856557572853646	18.097416549014667	13.013880743454475	25.032145134677208
2	15.945330611637157	19.77357288414958	39.22095775311168	25.06013875110158
3	18.77644567374747	23.92115501545016	29.60648144942029	27.69591786138208
4	13.480288448553706	15.810307610590296	36.35340280235263	34.356001138503366
5	14.422564880992365	36.94617355011921	34.351903339664986	14.279358229223435
6	33.5638358986001	35.04835224464879	17.190032765463236	14.197779091287874
7	28.820934251864138	31.7094196935016	21.387749338822555	18.08189671581171
8	26.571714537269298	34.37664390805744	20.291224368877742	18.76041718579553
9	26.977400396782965	15.408785770074203	19.482991728324443	38.13082210481839
10-11	25.55187257900913	25.01089262039244	28.095087471462026	21.342147329136402
12-13	26.11132213749441	22.687607021381346	27.71716850086569	23.483902340258556
14-15	23.372273911451146	24.255515870351	24.94795499656264	27.424255221635214
16-17	23.55522897573674	27.244550271381023	25.858562749214446	23.341658003667792
18-19	23.64677452119352	26.637015842496258	27.0499056454574	22.666303990852818
20-21	24.11492093146563	25.937556078585555	26.858919127606264	23.08860386234255
22-23	24.093865121459224	25.586181054766772	26.634422922653954	23.68553090112005
24-25	23.91279892253412	25.82797652198805	26.514286426345997	23.744938129131835
26-27	23.606791817963302	26.035625220314955	26.566094313576905	23.79148864814484
28-29	23.758163109398687	26.278771061951296	26.415364338426713	23.547701490223304
30-31	23.981756543502456	25.984155504737018	26.25460966007564	23.779478291684892
32-33	23.779044165039465	26.02691135414134	26.110190687699035	24.083853793120156
34-35	24.132794605524104	26.085457982656756	26.163872500623754	23.617874911195386
36-37	24.26630896004227	25.848202259496595	26.247404098043127	23.638084682418008
38-39	23.952202937157264	25.907891235417246	26.27779559907412	23.862110228351362
40-41	24.06923470870763	25.826106966379225	26.355108170581477	23.749550154331665
42-43	24.00714998354512	25.852003871491718	26.069610558588565	24.071235586374595
44-45	23.893342504235157	25.894513943647834	26.234141235932718	23.97800231618429
46-47	24.11746707820535	25.815506202116804	26.069935573566873	23.997091146110968
48-49	23.85434741642091	25.94733313697001	26.06515113243793	24.13316831417115
50-51	23.916435462663696	26.02250241171561	26.16672863887365	23.894333486747048
52-53	24.152871758670475	25.817206142802263	26.106996292849622	23.92292580567764
54-55	23.656562105398923	25.70595671237018	26.40246553513887	24.235015647092027
56-57	23.738384476136883	25.83383347566153	26.26688565446181	24.16089639373978
58-59	23.811752076108338	25.613262266242227	26.365982111634374	24.20900354601506
60-61	23.709005290702564	25.747473197559152	26.511508556755636	24.032012954982648
62-63	23.836140639987214	25.770314047477843	26.416483154339833	23.97706215819511
64-65	23.59401260822872	25.6104450845157	26.40953253514156	24.386009772114022
66-67	23.648566774278716	25.671722982560123	26.28801186292387	24.39169838023729
68-69	23.76396482568767	25.595346332184004	26.38853473705158	24.252154105076748
70-71	23.63580740157835	25.461710325844862	26.310009342538365	24.59247293003843
72-73	23.639975626943148	25.649452689660436	26.241114711982753	24.469456971413663
74-75	23.696412830844785	25.763843324946194	26.282430091068175	24.257313753140842
76-77	23.75186230673828	25.4402497462756	26.295346464888965	24.512541482097156
78-79	23.685239783179377	25.5483708113922	26.323584634487073	24.442804770941347
80-81	23.905924439037086	25.629129967165724	26.19478733145278	24.27015826234441
82-83	23.77590113442409	25.612586926624115	26.284853007572767	24.32665893137903
84-85	23.70052695654767	25.676154566060976	26.314296622061857	24.3090218553295
86-87	23.799827008340223	25.696700673099865	26.198664343464053	24.30480797509586
88-89	23.67874492298473	25.45909881591797	26.304557106711645	24.557599154385652
90-91	23.69327604898949	25.459735991682024	26.350387772187915	24.496600187140572
92-93	23.86728542290144	25.266648321074907	26.444580967090126	24.421485288933535
94-95	23.7468522829857	25.147367431736896	26.5326230241042	24.573157261173204
96-97	23.8405710519119	24.917917877471545	26.54752525847219	24.693985812144355
98-99	23.703188756024296	24.986556802084742	26.443832402988317	24.86642203890265
100-101	23.92634557860691	24.854985617784937	26.551786986102655	24.666881817505498
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	100.0
1	82.0
2	105.5
3	155.0
4	136.0
5	134.5
6	222.0
7	483.5
8	744.0
9	796.0
10	703.5
11	641.5
12	678.5
13	741.0
14	846.5
15	933.5
16	1001.0
17	1072.5
18	1233.5
19	1416.0
20	1536.0
21	1691.5
22	1874.5
23	2017.0
24	2333.5
25	2839.5
26	3499.0
27	4648.5
28	6379.0
29	8851.0
30	12503.5
31	17847.0
32	25318.5
33	30816.5
34	34619.0
35	40680.0
36	48923.5
37	57342.5
38	65026.5
39	72317.5
40	78949.5
41	86209.0
42	93386.0
43	100217.5
44	106851.0
45	114083.0
46	119509.0
47	122903.0
48	125461.5
49	126303.0
50	125736.0
51	124596.0
52	123713.0
53	121923.5
54	119200.0
55	113326.5
56	103549.0
57	92336.5
58	83544.0
59	73443.5
60	60081.5
61	49561.0
62	41780.5
63	32628.0
64	23513.0
65	17420.0
66	13309.0
67	10531.0
68	8165.5
69	6148.0
70	4674.0
71	3485.0
72	2537.5
73	1902.5
74	1458.5
75	1113.0
76	808.0
77	560.0
78	392.5
79	286.0
80	213.0
81	148.5
82	122.5
83	104.5
84	73.0
85	63.5
86	63.5
87	48.5
88	42.0
89	33.5
90	28.5
91	27.0
92	20.5
93	20.0
94	19.5
95	14.0
96	10.0
97	5.5
98	4.5
99	5.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05003619492385225
2	0.06931016489106227
3	0.06616127572227212
4	0.06595365665619805
5	0.06138603720256838
6	0.009688889750123534
7	0.012664763030518618
8	0.015640636310913704
9	0.005778730672395108
10-11	0.011661270877827253
12-13	0.020433176419456953
14-15	0.0367485746951114
16-17	0.02437793867486439
18-19	0.00951587386172847
20-21	0.01894523977925941
22-23	0.03375539982587681
24-25	0.03138508215486444
26-27	0.0200179382873088
28-29	0.01064047713629638
30-31	0.011211429568000088
32-33	0.008149048343407472
34-35	0.004740635342024729
36-37	5.709524317037083E-4
38-39	0.00370254001165435
40-41	9.688889750123533E-4
42-43	0.21678890815901405
44-45	0.18763573096444594
46-47	0.1941411283681003
48-49	0.20064652577175468
50-51	0.19206493770735955
52-53	0.19903747800968058
54-55	0.1960616047292855
56-57	0.19824160492306328
58-59	0.20645985962182878
60-61	0.2102316059888412
62-63	0.19649414445027316
64-65	0.1965633508056312
66-67	0.1947293823886435
68-69	0.18940049302607556
70-71	0.18177049234785328
72-73	0.19293001714933486
74-75	0.1913728741537793
76-77	0.1861823975019274
78-79	0.202947637087409
80-81	0.21396874917817454
82-83	0.21294795543664366
84-85	0.2072384311196066
86-87	0.22602795659931044
88-89	0.2188304956420758
90-91	0.2254050994010882
92-93	0.22082017835861903
94-95	0.22384795640553262
96-97	0.23071668717481666
98-99	0.22421128977116225
100-101	0.23043986175338452
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2889908.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.83995380188992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9444577833657	44.504638835013424
2	12.541092626303657	14.507524362612518
3	4.221919901381568	7.325869561535686
4	1.9944976004095654	4.614465962626784
5	1.1206364488349116	3.2408780214662634
6	0.6848484741323352	2.3766962463065444
7	0.4626487362823902	1.8731707073153345
8	0.3546046660268732	1.6408254000743159
9	0.2657047602474971	1.3831515951891724
>10	1.3355694160142575	13.412467818275307
>50	0.054542517525780806	2.1307386330840123
>100	0.01707882644660691	1.6540319037219764
>500	0.001438945817501766	0.561654063030235
>1k	9.592972112496341E-4	0.773886889748449
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.460317767901262E-5
2	0.0	0.0	0.0	0.0	3.460317767901262E-5
3	0.0	0.0	0.0	0.0	3.460317767901262E-5
4	0.0	0.0	0.0	0.0	3.460317767901262E-5
5	0.0	0.0	0.0	3.460317767901262E-5	3.460317767901262E-5
6	0.0	0.0	0.0	3.460317767901262E-5	3.460317767901262E-5
7	0.0	0.0	0.0	3.460317767901262E-5	3.460317767901262E-5
8	0.0	0.0	0.0	3.460317767901262E-5	3.460317767901262E-5
9	0.0	0.0	0.0	3.460317767901262E-5	3.460317767901262E-5
10-11	0.0	0.0	0.0	3.460317767901262E-5	3.460317767901262E-5
12-13	0.0	0.0	0.0	3.460317767901262E-5	2.0761906607407571E-4
14-15	0.0	0.0	0.0	3.460317767901262E-5	2.24920654913582E-4
16-17	0.0	0.0	0.0	5.190476651851893E-5	2.4222224375308832E-4
18-19	0.0	0.0	0.0	6.920635535802523E-5	2.5952383259259464E-4
20-21	0.0	0.0	0.0	6.920635535802523E-5	2.941270102716073E-4
22-23	0.0	0.0	0.0	6.920635535802523E-5	3.1142859911111357E-4
24-25	0.0	0.0	0.0	2.941270102716073E-4	3.1142859911111357E-4
26-27	0.0	0.0	0.0	9.342857973333406E-4	3.1142859911111357E-4
28-29	0.0	3.460317767901262E-5	0.0	0.0034603177679012618	3.1142859911111357E-4
30-31	0.0	3.460317767901262E-5	0.0	0.013253017051061834	3.1142859911111357E-4
32-33	0.0	3.460317767901262E-5	0.0	0.03998397180809908	3.1142859911111357E-4
34-35	0.0	3.460317767901262E-5	0.0	0.07604048294963023	3.1142859911111357E-4
36-37	0.0	3.460317767901262E-5	0.0	0.1318727101347171	3.1142859911111357E-4
38-39	0.0	3.460317767901262E-5	0.0	0.2201973211603968	3.1142859911111357E-4
40-41	0.0	3.460317767901262E-5	0.0	0.3187471711902248	3.1142859911111357E-4
42-43	0.0	3.460317767901262E-5	0.0	0.4252903552639046	3.2873018795061986E-4
44-45	0.0	3.460317767901262E-5	0.0	0.556799732032992	3.8063495446913885E-4
46-47	0.0	3.460317767901262E-5	0.0	0.7138808571068698	4.49841309827164E-4
48-49	0.0	3.460317767901262E-5	0.0	0.8717059505008464	4.8444448750617665E-4
50-51	0.0	3.460317767901262E-5	0.0	1.0351540602676625	4.8444448750617665E-4
52-53	0.0	3.460317767901262E-5	0.0	1.2044674086510714	5.190476651851893E-4
54-55	0.0	3.460317767901262E-5	0.0	1.4032799660058384	5.882540205432146E-4
56-57	0.0	3.460317767901262E-5	0.0	1.6308996687783832	6.055556093827209E-4
58-59	0.0	3.460317767901262E-5	0.0	1.86827746765641	6.228571982222271E-4
60-61	0.0	3.460317767901262E-5	0.0	2.1187871724636214	6.228571982222271E-4
62-63	0.0	3.460317767901262E-5	0.0	2.376632750938784	6.920635535802524E-4
64-65	0.0	3.460317767901262E-5	0.0	2.6665900783000707	7.26666731259265E-4
66-67	0.0	3.460317767901262E-5	0.0	3.0010816953342463	7.26666731259265E-4
68-69	0.0	3.460317767901262E-5	0.0	3.361802521049113	7.26666731259265E-4
70-71	0.0	3.460317767901262E-5	0.0	3.7382850941967702	7.439683200987713E-4
72-73	0.0	3.460317767901262E-5	0.0	4.133020843570106	8.304762642963029E-4
74-75	0.0	3.460317767901262E-5	0.0	4.551580880775443	8.304762642963029E-4
76-77	0.0	3.460317767901262E-5	0.0	5.017616477756386	8.304762642963029E-4
78-79	0.0	3.460317767901262E-5	0.0	5.543602080066217	8.304762642963029E-4
80-81	0.0	3.460317767901262E-5	0.0	6.07548752417032	8.477778531358091E-4
82-83	0.0	3.460317767901262E-5	0.0	6.631162652928744	8.650794419753154E-4
84-85	0.0	6.920635535802523E-5	0.0	7.2159736572928965	8.650794419753154E-4
86-87	0.0	6.920635535802523E-5	0.0	7.851426412190284	8.650794419753154E-4
88-89	0.0	6.920635535802523E-5	0.0	8.532520758446289	8.650794419753154E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4100	0.0	45.264492	1
GGTATCA	2160	0.0	45.04689	1
TCAACGC	4995	0.0	35.824955	4
CAACGCA	5180	0.0	34.44968	5
ATCAACG	5235	0.0	34.363293	3
TATCAAC	5325	0.0	34.051678	2
AACGCAG	5540	0.0	32.281094	6
GTACATG	7490	0.0	28.38971	1
ACGCAGA	6370	0.0	27.929848	7
TACATGG	7675	0.0	27.027	2
CGCAGAG	6475	0.0	26.892153	8
ACATGGG	8015	0.0	25.464619	3
CATGGGG	6865	0.0	23.162426	4
GCAGAGT	7560	0.0	21.653429	9
AGAGTAC	6355	0.0	19.674421	10-11
TAAGGCG	1255	0.0	19.286507	5
ATGGGGG	4275	0.0	18.539904	5
GAGTACA	5765	0.0	18.3599	1
GTATTAA	2180	0.0	18.288898	1
GTATAGG	865	0.0	17.558952	1
>>END_MODULE