FastQCFastQC Report
Mon 15 Aug 2016
SRR2921708_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921708_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2889908
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23750.047.0290261
GTATCAA43800.042.8632161
TCAACGC54600.033.985334
CAACGCA55550.033.4284175
ATCAACG56750.032.614153
TATCAAC57450.032.0515482
AACGCAG59550.031.1000356
GTACATG77950.026.584721
ACGCAGA70000.025.914087
TACATGG79550.025.354562
CGCAGAG74050.024.0478768
ACATGGG83150.023.914383
CATGGGG72750.021.7881724
GCAGAGT85050.019.877139
AGAGTAC72350.019.59222810-11
ATGGGGG43550.016.9031125
CAGAGTA78450.016.82789810-11
GTATATG7650.016.775061
GAGTACT36900.016.39460612-13
TTAATAC24400.015.9489223
Produced by FastQC (version 0.11.5)