##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921708_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2889908
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13252567209752	33.0	31.0	34.0	28.0	34.0
2	31.27951443436954	33.0	31.0	34.0	28.0	34.0
3	31.349527043767484	34.0	31.0	34.0	28.0	34.0
4	34.83363345822774	37.0	35.0	37.0	32.0	37.0
5	34.55304840154081	37.0	35.0	37.0	32.0	37.0
6	34.520073303371596	37.0	35.0	37.0	31.0	37.0
7	34.39323293336674	37.0	35.0	37.0	31.0	37.0
8	34.334838686906295	37.0	35.0	37.0	30.0	37.0
9	35.89813793380274	39.0	37.0	39.0	30.0	39.0
10-11	35.783232026763486	39.0	37.0	39.0	30.0	39.0
12-13	35.69680038257273	39.0	37.0	39.0	30.0	39.0
14-15	36.90203286748229	40.0	37.0	41.0	30.0	41.0
16-17	36.86366642121479	40.0	37.0	41.0	30.0	41.0
18-19	36.82379906211547	40.0	37.0	41.0	30.0	41.0
20-21	36.76156455499621	40.0	37.0	41.0	30.0	41.0
22-23	36.66541322422721	40.0	37.0	41.0	30.0	41.0
24-25	36.57290232076592	40.0	37.0	41.0	30.0	41.0
26-27	36.43847312786428	40.0	37.0	41.0	29.5	41.0
28-29	36.305242243005665	40.0	37.0	41.0	29.0	41.0
30-31	36.13924024571024	40.0	36.0	41.0	28.5	41.0
32-33	36.07517367334877	40.0	36.0	41.0	28.0	41.0
34-35	36.19800422712419	40.0	37.0	41.0	29.0	41.0
36-37	36.18047910175687	40.0	36.5	41.0	28.5	41.0
38-39	36.06770699966919	40.0	36.0	41.0	28.0	41.0
40-41	35.97135929586686	40.0	36.0	41.0	27.5	41.0
42-43	35.83485079801848	40.0	36.0	41.0	27.0	41.0
44-45	35.693159609233234	40.0	35.5	41.0	26.5	41.0
46-47	35.524641960920555	40.0	35.0	41.0	26.0	41.0
48-49	35.36996160431404	40.0	35.0	41.0	25.5	41.0
50-51	35.206316948498014	39.0	35.0	41.0	24.5	41.0
52-53	35.02523661652897	39.0	35.0	41.0	24.0	41.0
54-55	34.8047839239173	39.0	35.0	41.0	24.0	41.0
56-57	34.57390563990272	39.0	34.5	41.0	23.0	41.0
58-59	34.33410942493671	38.0	34.0	40.0	23.0	41.0
60-61	34.051049375966294	38.0	34.0	40.0	22.0	41.0
62-63	33.746925853695	37.0	34.0	40.0	22.0	41.0
64-65	33.40633110119769	37.0	34.0	40.0	20.5	41.0
66-67	33.056877935214544	36.0	33.0	39.0	20.0	41.0
68-69	32.699349252640566	36.0	33.0	39.0	18.5	40.5
70-71	32.306809766954515	35.0	33.0	38.5	17.0	40.0
72-73	31.94678117780912	35.0	32.5	37.5	13.5	39.5
74-75	31.550161112395273	35.0	32.0	37.0	9.5	39.0
76-77	28.871722560026132	32.0	28.0	34.5	6.0	36.5
78-79	30.432168774922935	34.5	31.0	35.5	7.0	37.0
80-81	30.617643191409552	35.0	31.5	35.5	3.5	37.0
82-83	30.444248225203015	35.0	32.0	35.0	2.0	37.0
84-85	30.248920034824636	35.0	31.0	35.0	2.0	36.0
86-87	30.04225221010496	35.0	31.0	35.0	2.0	36.0
88-89	29.773362335409985	34.0	31.0	35.0	2.0	36.0
90-91	29.578104562498183	34.0	31.0	35.0	2.0	35.0
92-93	29.416585406871086	34.0	31.0	35.0	2.0	35.0
94-95	29.307366878115154	34.0	31.0	35.0	2.0	35.0
96-97	29.098479778595028	34.0	31.0	35.0	2.0	35.0
98-99	28.889805488617633	34.0	31.0	35.0	2.0	35.0
100-101	27.76980305255392	33.0	27.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	67916.0
3	21906.0
4	11529.0
5	8364.0
6	7095.0
7	7200.0
8	7544.0
9	8059.0
10	8756.0
11	9335.0
12	10016.0
13	10709.0
14	11432.0
15	12306.0
16	13384.0
17	13918.0
18	13247.0
19	11745.0
20	11370.0
21	11266.0
22	11807.0
23	13302.0
24	15674.0
25	18627.0
26	22531.0
27	26708.0
28	32285.0
29	38627.0
30	47278.0
31	58656.0
32	74185.0
33	96896.0
34	138247.0
35	207768.0
36	362539.0
37	683781.0
38	684470.0
39	79430.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.12497825304926	19.972018345762308	13.75478172992748	26.148221671260963
2	14.899882418158125	21.444775635990425	37.378360927117946	26.276981018733508
3	17.214978116567906	25.29816111341629	29.418032557120654	28.068828212895152
4	13.072963437823883	17.242826764391893	35.204404556127116	34.47980524165711
5	14.080375131479805	37.44171018753459	34.33856183678195	14.139352844203657
6	33.45693840750849	35.82315200552001	16.845516841883434	13.874392745088066
7	28.52969713914768	32.002679670079466	21.408363172806887	18.05926001796597
8	26.462088066471317	35.02533644669658	19.965583679480453	18.546991807351652
9	26.698531579552014	15.484056931916172	19.32961187691788	38.48779961161393
10-11	25.43852603876553	25.391080860362315	28.039165726475517	21.131227374396637
12-13	25.98968372786537	22.888071751951323	27.78403616318278	23.338208357000525
14-15	23.20607326929612	24.509547100157867	24.90589242495444	27.378487205591572
16-17	23.454604783266454	27.54598070249987	25.76671990942272	23.23269460481095
18-19	23.518239862720254	26.883309967193973	27.047833195005094	22.550616975080672
20-21	24.00614276885514	26.04870338163716	26.855685763738702	23.089468085769
22-23	23.909433087391204	25.80913056905801	26.639295548386933	23.64214079516386
24-25	23.769147521668156	26.047653939283315	26.55997314783656	23.62322539121197
26-27	23.50869920059436	26.247022104553047	26.586089964667526	23.658188730185067
28-29	23.638584952638084	26.457062830973687	26.43628262328235	23.468069593105884
30-31	23.947801346375442	26.12099174880856	26.254338394887768	23.676868509928234
32-33	23.707136711939462	26.187831231653995	26.070133035990523	24.034899020416017
34-35	24.030515346612088	26.257930086502483	26.162589094513205	23.54896547237222
36-37	24.137065924656202	25.981151333142893	26.281309838565313	23.600472903635588
38-39	23.91143593498478	26.07027628561186	26.261787572476358	23.756500206927004
40-41	24.03375852151268	26.053297118832898	26.238533665695613	23.67441069395881
42-43	23.98633333079555	26.044552677168024	25.956566486414655	24.01254750562177
44-45	23.81650339254205	26.022339180841335	26.198273120484643	23.962884306131976
46-47	24.025647307648654	26.03096529329203	25.974819850121943	23.968567548937372
48-49	23.76104150481111	26.185076816862857	26.010696741663825	24.043184936662207
50-51	23.845348902393702	26.19475720750742	26.055285528511323	23.904608361587552
52-53	24.163700692023262	25.97430774748738	25.9862633460208	23.87572821446856
54-55	23.581154814617054	25.919509334333636	26.23465350491913	24.264682346130176
56-57	23.669464370158543	25.98189114651166	26.108825111607935	24.23981937172186
58-59	23.690253297294678	25.843124565234692	26.16402894628464	24.302593191185988
60-61	23.629222896688333	25.976219619177538	26.30796766300088	24.08658982113325
62-63	23.66900706862189	25.99017726844583	26.24378378499982	24.09703187793246
64-65	23.41190229711278	25.77734845368034	26.23157157947555	24.57917766973134
66-67	23.49170785990111	25.916664156467466	26.108270258086563	24.48335772554486
68-69	23.59292642015998	25.799231087469426	26.126598799720963	24.48124369264963
70-71	23.464321546269755	25.66402324113806	26.10228766967944	24.769367542912743
72-73	23.408579167351743	25.90640278991684	25.982710130755716	24.7023079119757
74-75	23.45823465226334	26.02985828864743	25.92484461246786	24.587062446621367
76-77	23.470346189851732	25.643697216134708	26.095574872613014	24.790381721400546
78-79	23.35846377614628	25.766016761324284	26.139112065383017	24.736407397146422
80-81	23.567618027318883	25.856922292254364	25.93541846214681	24.640041218279947
82-83	23.42402258933116	25.84236236832856	26.044117104970095	24.689497937370195
84-85	23.286522124770034	25.858253574022616	26.066511752539917	24.788712548667434
86-87	23.37157255404777	25.921548368153292	25.91098759819352	24.795891479605423
88-89	23.142836074035838	25.74358129063881	26.04217110485519	25.07141153047016
90-91	23.034373765310153	25.728416038140672	26.081803033686633	25.15540716286254
92-93	23.20327521968317	25.557560071428618	26.15318517226027	25.08597953662794
94-95	22.978042323464777	25.475991902133476	26.25496167355908	25.291004100842663
96-97	22.9786049277034	25.266977687128367	26.30095546994735	25.45346191522088
98-99	22.799394162953142	25.312486374895066	26.207919620358727	25.680199841793065
100-101	22.739967396829584	25.33953561763146	26.235984391171606	25.684512594367355
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	93.0
1	79.0
2	95.5
3	119.5
4	111.5
5	133.0
6	208.5
7	435.5
8	654.5
9	689.0
10	579.5
11	477.0
12	491.0
13	522.0
14	576.5
15	615.0
16	640.5
17	748.0
18	845.0
19	914.5
20	1051.5
21	1223.0
22	1447.0
23	1723.0
24	2070.5
25	2636.0
26	3521.5
27	4828.0
28	6758.0
29	9615.0
30	13482.0
31	18828.0
32	26375.5
33	31807.0
34	35500.5
35	41375.0
36	49260.0
37	57713.0
38	65407.0
39	72685.5
40	79831.5
41	87142.5
42	94217.5
43	101446.0
44	108156.0
45	114995.5
46	120080.0
47	123771.0
48	126835.5
49	127553.0
50	126763.5
51	125299.0
52	123796.5
53	121913.0
54	118914.0
55	112566.0
56	102904.0
57	91901.5
58	82837.5
59	72413.0
60	59095.0
61	48883.0
62	41089.5
63	31530.5
64	22478.5
65	16690.0
66	12650.5
67	9860.5
68	7560.0
69	5618.5
70	4212.0
71	3079.5
72	2179.0
73	1583.0
74	1219.0
75	895.0
76	631.0
77	406.0
78	225.0
79	142.5
80	83.0
81	48.5
82	35.5
83	25.5
84	19.5
85	12.0
86	8.0
87	6.0
88	4.5
89	3.0
90	2.5
91	2.5
92	1.5
93	0.0
94	0.5
95	0.5
96	0.5
97	0.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1538457279608901
2	2.0761906607407571E-4
3	0.00965428657244452
4	0.005986349738469183
5	0.023564763999407594
6	0.0020415874830617448
7	0.0
8	0.0
9	0.0
10-11	0.045364765937185546
12-13	2.5952383259259464E-4
14-15	0.0044984130982716405
16-17	0.0
18-19	0.0014706350513580362
20-21	2.0761906607407571E-4
22-23	1.730158883950631E-5
24-25	3.633333656296325E-4
26-27	0.0025433335594074276
28-29	0.0048098416973827544
30-31	0.015450318833679135
32-33	0.010865397791209963
34-35	0.009688889750123534
36-37	0.003512222534419781
38-39	0.0
40-41	0.0030969844022716297
42-43	0.008650794419753156
44-45	0.006470794225975359
46-47	0.003183492346469161
48-49	0.008408572176000067
50-51	0.0025606351482469337
52-53	0.0016782541174321121
54-55	0.004135079732642008
56-57	0.007491587967506232
58-59	0.014464128269827273
60-61	0.007439683200987713
62-63	0.02039857324177794
64-65	0.039084289188444754
66-67	0.058513973455210345
68-69	0.04889429006044484
70-71	0.06662841862093881
72-73	0.032284764774518776
74-75	0.026315716624889098
76-77	0.21708303516928565
78-79	0.07986413408316112
80-81	0.06467333908207458
82-83	0.026177303914173045
84-85	0.04934413137027199
86-87	0.06439651366064249
88-89	0.28359034266834793
90-91	0.2894382796961011
92-93	0.04948254408098804
94-95	0.008927619841185256
96-97	0.01340873135061739
98-99	7.612699089382776E-4
100-101	1.730158883950631E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2889908.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.41133849461729
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.51961670234934	43.93093615976949
2	12.67581586937149	14.554711111438563
3	4.3954092462770555	7.5703898418114814
4	1.9903429832983703	4.570730189381165
5	1.1299440575910589	3.243580038517081
6	0.7246614712402332	2.496227101162824
7	0.49209849581119297	1.9776423320995884
8	0.34812987510342736	1.5989281679721368
9	0.27626165341282505	1.4274496167449708
>10	1.3722149660216347	13.578097644499218
>50	0.05674049073536596	2.187093764778378
>100	0.01659064697308938	1.6219006250884065
>500	0.001267899392159943	0.5184162754443322
>1k	9.056424228894602E-4	0.7238971312924976
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	6.920635535802523E-5
14-15	0.0	0.0	0.0	0.0	6.920635535802523E-5
16-17	0.0	0.0	0.0	1.730158883950631E-5	6.920635535802523E-5
18-19	0.0	0.0	0.0	3.460317767901262E-5	6.920635535802523E-5
20-21	3.460317767901262E-5	0.0	0.0	3.460317767901262E-5	1.2111112187654416E-4
22-23	3.460317767901262E-5	0.0	0.0	3.460317767901262E-5	1.9031747723456942E-4
24-25	3.460317767901262E-5	0.0	0.0	3.2873018795061986E-4	2.0761906607407571E-4
26-27	3.460317767901262E-5	3.460317767901262E-5	0.0	9.688889750123534E-4	2.24920654913582E-4
28-29	3.460317767901262E-5	3.460317767901262E-5	0.0	0.003494920945580274	2.4222224375308832E-4
30-31	3.460317767901262E-5	3.460317767901262E-5	0.0	0.013062699573827264	2.4222224375308832E-4
32-33	3.460317767901262E-5	3.460317767901262E-5	0.0	0.03968984479782747	2.7682542143210093E-4
34-35	3.460317767901262E-5	3.460317767901262E-5	0.0	0.0756944511728401	3.1142859911111357E-4
36-37	3.460317767901262E-5	3.460317767901262E-5	0.0	0.13147477359140847	3.1142859911111357E-4
38-39	3.460317767901262E-5	3.460317767901262E-5	0.0	0.21947065442913755	3.460317767901262E-4
40-41	3.460317767901262E-5	3.460317767901262E-5	0.0	0.3173111393165457	3.460317767901262E-4
42-43	3.460317767901262E-5	3.460317767901262E-5	0.0	0.42454638694380586	3.806349544691388E-4
44-45	3.460317767901262E-5	3.460317767901262E-5	0.0	0.5552425890374365	3.806349544691388E-4
46-47	3.460317767901262E-5	5.190476651851893E-5	0.0	0.711320221958623	3.806349544691388E-4
48-49	5.190476651851893E-5	6.920635535802523E-5	0.0	0.8687300772204514	3.806349544691388E-4
50-51	6.920635535802523E-5	8.650794419753154E-5	0.0	1.0313823139006502	4.1523813214815143E-4
52-53	6.920635535802523E-5	1.0380953303703786E-4	0.0	1.1998997891974414	4.844444875061767E-4
54-55	6.920635535802523E-5	1.0380953303703786E-4	0.0	1.3967572670133444	5.190476651851893E-4
56-57	6.920635535802523E-5	1.0380953303703786E-4	0.0	1.6236676046434697	5.53650842864202E-4
58-59	6.920635535802523E-5	1.0380953303703786E-4	0.0	1.8584155620178913	5.882540205432146E-4
60-61	6.920635535802523E-5	1.0380953303703786E-4	0.0	2.107800663550535	6.228571982222271E-4
62-63	6.920635535802523E-5	1.0380953303703786E-4	0.0	2.363570051364957	6.574603759012397E-4
64-65	6.920635535802523E-5	1.0380953303703786E-4	0.0	2.6518491246088107	6.574603759012397E-4
66-67	6.920635535802523E-5	1.0380953303703786E-4	0.0	2.984143439860369	6.747619647407461E-4
68-69	6.920635535802523E-5	1.0380953303703786E-4	0.0	3.343030297158249	7.26666731259265E-4
70-71	6.920635535802523E-5	1.0380953303703786E-4	0.0	3.716710012913906	7.439683200987713E-4
72-73	6.920635535802523E-5	1.0380953303703786E-4	0.0	4.110615286022946	8.477778531358091E-4
74-75	6.920635535802523E-5	1.0380953303703786E-4	0.0	4.530040402670258	8.823810308148218E-4
76-77	6.920635535802523E-5	1.0380953303703786E-4	0.0	4.995556951986015	8.996826196543281E-4
78-79	6.920635535802523E-5	1.0380953303703786E-4	0.0	5.520867792331105	9.342857973333407E-4
80-81	6.920635535802523E-5	1.0380953303703786E-4	0.0	6.054500696907999	9.51587386172847E-4
82-83	6.920635535802523E-5	1.0380953303703786E-4	0.0	6.610158524077583	0.001003492152691366
84-85	6.920635535802523E-5	1.2111112187654416E-4	0.0	7.195782703117192	0.001003492152691366
86-87	6.920635535802523E-5	1.3841271071605047E-4	0.0	7.8331905375534445	0.0010380953303703786
88-89	6.920635535802523E-5	1.3841271071605047E-4	0.0	8.51636107447019	0.0010726985080493912
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2375	0.0	47.029026	1
GTATCAA	4380	0.0	42.863216	1
TCAACGC	5460	0.0	33.98533	4
CAACGCA	5555	0.0	33.428417	5
ATCAACG	5675	0.0	32.61415	3
TATCAAC	5745	0.0	32.051548	2
AACGCAG	5955	0.0	31.100035	6
GTACATG	7795	0.0	26.58472	1
ACGCAGA	7000	0.0	25.91408	7
TACATGG	7955	0.0	25.35456	2
CGCAGAG	7405	0.0	24.047876	8
ACATGGG	8315	0.0	23.91438	3
CATGGGG	7275	0.0	21.788172	4
GCAGAGT	8505	0.0	19.87713	9
AGAGTAC	7235	0.0	19.592228	10-11
ATGGGGG	4355	0.0	16.903112	5
CAGAGTA	7845	0.0	16.827898	10-11
GTATATG	765	0.0	16.77506	1
GAGTACT	3690	0.0	16.394606	12-13
TTAATAC	2440	0.0	15.948922	3
>>END_MODULE